Restriction map for
pF1KB8345
(Circular) (Six-base) MAPSORT of: pF1KB8345.seq Check: 9145 from: 1 to: 4997
pF1KB8345 4997 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 2035 2035
Size: 4997
Acc65I G'GTAC_C
Cuts at: 2013 2013
Size: 4997
AclI AA'CG_TT
Cuts at: 916 4837 916
Size: 3921 1076
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1053 1473 1553 1916 2822 3254 4001 4269 1053
Size: 420 80 363 906 432 747 268 1781
Fragments arranged by size:
1781 906 747 432 420 363 268 80
AflII C'TTAA_G
Cuts at: 1842 1842
Size: 4997
AflIII A'CryG_T
Cuts at: 545 1515 1683 1851 2349 3453 4430 545
Size: 970 168 168 498 1104 977 1112
Fragments arranged by size:
1112 1104 977 970 498 168 168
AgeI A'CCGG_T
Cuts at: 3385 3385
Size: 4997
AlwNI CAG_nnn'CTG
Cuts at: 225 2302 3869 225
Size: 2077 1567 1353
Fragments arranged by size:
2077 1567 1353
ApaLI G'TGCA_C
Cuts at: 1357 3767 1357
Size: 2410 2587
ApoI r'AATT_y
Cuts at: 365 512 1915 2001 4350 4606 365
Size: 147 1403 86 2349 256 756
Fragments arranged by size:
2349 1403 756 256 147 86
AseI AT'TA_AT
Cuts at: 20 20
Size: 4997
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4997
AvaI C'yCGr_G
Cuts at: 2017 2017
Size: 4997
BamHI G'GATC_C
Cuts at: 2022 2022
Size: 4997
BanI G'GyrC_C
Cuts at: 2013 2427 2675 2710 2013
Size: 414 248 35 4300
Fragments arranged by size:
4300 414 248 35
BanII G_rGCy'C
Cuts at: 2011 3041 2011
Size: 1030 3967
BbeI G_GCGC'C
Cuts at: 2431 2679 2431
Size: 248 4749
BbsI GAAGACnn'nnnn_
Cuts at: 552 930 1014 1098 1182 1237 1266 1541 1731
Size: 378 84 84 84 55 29 275 190
Cuts at: 1731 552
Size: 3818
Fragments arranged by size:
3818 378 275 190 84 84 84 55
29
BbvCI CC'TCA_GC
Cuts at: 478 478
Size: 4997
BciVI GTATCCnnnnn_n'
Cuts at: 2895 3656 4997 2895
Size: 761 1341 2895
Fragments arranged by size:
2895 1341 761
BclI T'GATC_A
Cuts at: 459 1961 2517 459
Size: 1502 556 2939
Fragments arranged by size:
2939 1502 556
BfrBI ATG'CAT
Cuts at: 567 675 567
Size: 108 4889
BglII A'GATC_T
Cuts at: 1956 2512 4549 1956
Size: 556 2037 2404
Fragments arranged by size:
2404 2037 556
BlpI GC'TnA_GC
Cuts at: 2109 2109
Size: 4997
Bme1580I G_kGCm'C
Cuts at: 1361 2622 2715 3771 1361
Size: 1261 93 1056 2587
Fragments arranged by size:
2587 1261 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1242 2209 2290 2625 4769 1242
Size: 967 81 335 2144 1470
Fragments arranged by size:
2144 1470 967 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 493 3364 493
Size: 2871 2126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 229 542 1049 1133 1147 1217 1385 1805
Size: 57 313 507 84 14 70 168 420
Cuts at: 1805 172
Size: 3364
Fragments arranged by size:
3364 507 420 313 168 84 70 57
14
Bpu10I CC'TnA_GC
Cuts at: 478 478
Size: 4997
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 813 2173 3052 3544 3842 568
Size: 245 1360 879 492 298 1723
Fragments arranged by size:
1723 1360 879 492 298 245
BsaI GGTCTCn'nnnn_
Cuts at: 205 693 4701 205
Size: 488 4008 501
Fragments arranged by size:
4008 501 488
BsaAI yAC'GTr
Cuts at: 548 2350 548
Size: 1802 3195
BsaBI GATnn'nnATC
Cuts at: 6 2511 4548 6
Size: 2505 2037 455
Fragments arranged by size:
2505 2037 455
BsaHI Gr'CG_yC
Cuts at: 2428 2676 3378 4874 2428
Size: 248 702 1496 2551
Fragments arranged by size:
2551 1496 702 248
BsaWI w'CCGG_w
Cuts at: 859 2186 2707 3385 3659 3806 4217 859
Size: 1327 521 678 274 147 411 1639
Fragments arranged by size:
1639 1327 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1318 1348 1318
Size: 30 4967
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 1323 169
Size: 1154 3843
BseYI C'CCAG_C
Cuts at: 2422 3180 3757 4253 2422
Size: 758 577 496 3166
Fragments arranged by size:
3166 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1762 1944 1762
Size: 182 4815
BsiEI CG_ry'CG
Cuts at: 104 2539 2585 3793 104
Size: 2435 46 1208 1308
Fragments arranged by size:
2435 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1361 2011 2789 2979 3771 1361
Size: 650 778 190 792 2587
Fragments arranged by size:
2587 792 778 650 190
BsiWI C'GTAC_G
Cuts at: 548 548
Size: 4997
BsmI GAATG_Cn'
Cuts at: 677 1135 1219 1303 1318 1529 1555 1822 677
Size: 458 84 84 15 211 26 267 3852
Fragments arranged by size:
3852 458 267 211 84 84 26 15
Bsp1286I G_dGCh'C
Cuts at: 1361 2011 2622 2715 2789 2979 3041 3771 1361
Size: 650 611 93 74 190 62 730 2587
Fragments arranged by size:
2587 730 650 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4217 4217
Size: 4997
BspHI T'CATG_A
Cuts at: 4173 4173
Size: 4997
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2047 2261 2563 2944 2047
Size: 214 302 381 4100
Fragments arranged by size:
4100 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3289 3343 4827 4992 50
Size: 3239 54 1484 165 55
Fragments arranged by size:
3239 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 808 1396 2909 808
Size: 588 1513 2896
Fragments arranged by size:
2896 1513 588
BsrFI r'CCGG_y
Cuts at: 2995 3176 3385 2995
Size: 181 209 4607
Fragments arranged by size:
4607 209 181
BsrGI T'GTAC_A
Cuts at: 503 503
Size: 4997
BssSI C'ACGA_G
Cuts at: 3268 3626 3268
Size: 358 4639
BstAPI GCAn_nnn'nTGC
Cuts at: 1307 2374 1307
Size: 1067 3930
BstBI TT'CG_AA
Cuts at: 921 1005 1089 1173 1257 3358 921
Size: 84 84 84 84 2101 2560
Fragments arranged by size:
2560 2101 84 84 84 84
BstXI CCAn_nnnn'nTGG
Cuts at: 637 637
Size: 4997
BstYI r'GATC_y
Cuts at: 1 1956 2022 2489 2512 2847 3093 4105 4191
Size: 1955 66 467 23 335 246 1012 86
Cuts at: 4191 4203 4549 1
Size: 12 346 449
Fragments arranged by size:
1955 1012 467 449 346 335 246 86
66 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4997
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 553 2947 3128 3291 553
Size: 2394 181 163 2259
Fragments arranged by size:
2394 2259 181 163
BtsI GCAGTG_nn'
Cuts at: 773 1480 1648 1932 2240 773
Size: 707 168 284 308 3530
Fragments arranged by size:
3530 707 308 284 168
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1122 1157 1206 1241 1374 1409 1542 1577 1626
Size: 35 49 35 133 35 133 35 49
Cuts at: 1626 1661 1122
Size: 35 4458
Fragments arranged by size:
4458 133 133 49 49 35 35 35
35 35
DraI TTT'AAA
Cuts at: 1996 1996
Size: 4997
DrdI GACnn_nn'nnGTC
Cuts at: 118 2703 3561 118
Size: 2585 858 1554
Fragments arranged by size:
2585 1554 858
EaeI y'GGCC_r
Cuts at: 1747 2582 2756 3147 3174 4519 1747
Size: 835 174 391 27 1345 2225
Fragments arranged by size:
2225 1345 835 391 174 27
EagI C'GGCC_G
Cuts at: 2582 2582
Size: 4997
EarI CTCTTCn'nnn_
Cuts at: 3020 3230 3020
Size: 210 4787
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3205 3515 3661 4807 3205
Size: 310 146 1146 3395
Fragments arranged by size:
3395 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2009 2009
Size: 4997
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 229 542 1049 1053 1133 1147 1217 1385
Size: 57 313 507 4 80 14 70 168
Cuts at: 1385 1473 1553 1805 1916 2822 3254 4001 4269
Size: 88 80 252 111 906 432 747 268
Cuts at: 4269 172
Size: 900
Fragments arranged by size:
906 900 747 507 432 313 268 252
168 111 88 80 80 70 57 14
4
EcoO109I rG'GnC_Cy
Cuts at: 186 2136 186
Size: 1950 3047
EcoRI G'AATT_C
Cuts at: 1915 2001 1915
Size: 86 4911
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3016 3016
Size: 4997
FspI TGC'GCA
Cuts at: 2778 2778
Size: 4997
HaeII r_GCGC'y
Cuts at: 2431 2679 3701 4680 2431
Size: 248 1022 979 2748
Fragments arranged by size:
2748 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 477 509 1946 1978 2839 2869 2871 2901 477
Size: 32 1437 32 861 30 2 30 2573
Fragments arranged by size:
2573 1437 861 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2036 2036
Size: 4997
HindIII A'AGCT_T
Cuts at: 896 1568 1652 896
Size: 672 84 4241
Fragments arranged by size:
4241 672 84
KasI G'GCGC_C
Cuts at: 2427 2675 2427
Size: 248 4749
KpnI G_GTAC'C
Cuts at: 2017 2017
Size: 4997
MfeI C'AATT_G
Cuts at: 1407 1827 1407
Size: 420 4577
MluI A'CGCG_T
Cuts at: 4430 4430
Size: 4997
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 416 3668 3852 416
Size: 3252 184 1561
Fragments arranged by size:
3252 1561 184
MscI TGG'CCA
Cuts at: 1749 2758 1749
Size: 1009 3988
MslI CAynn'nnrTG
Cuts at: 624 876 960 1044 1354 2986 3113 624
Size: 252 84 84 310 1632 127 2508
Fragments arranged by size:
2508 1632 310 252 127 84 84
MspA1I CmG'CkG
Cuts at: 2108 2422 2782 3795 4040 2108
Size: 314 360 1013 245 3065
Fragments arranged by size:
3065 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3178 3178
Size: 4997
NarI GG'CG_CC
Cuts at: 2428 2676 2428
Size: 248 4749
NcoI C'CATG_G
Cuts at: 107 107
Size: 4997
NdeI CA'TA_TG
Cuts at: 1013 1433 1601 1769 1013
Size: 420 168 168 4241
Fragments arranged by size:
4241 420 168 168
NgoMIV G'CCGG_C
Cuts at: 3176 3176
Size: 4997
NsiI A_TGCA'T
Cuts at: 569 677 569
Size: 108 4889
NspI r_CATG'y
Cuts at: 679 763 1519 1687 1855 2050 2550 3457 679
Size: 84 756 168 168 195 500 907 2219
Fragments arranged by size:
2219 907 756 500 195 168 168 84
PciI A'CATG_T
Cuts at: 1515 1683 1851 3453 1515
Size: 168 168 1602 3059
Fragments arranged by size:
3059 1602 168 168
PmeI GTTT'AAAC
Cuts at: 1996 1996
Size: 4997
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 214 246 4136 4168 214
Size: 32 3890 32 1043
Fragments arranged by size:
3890 1043 32 32
PpuMI rG'GwC_Cy
Cuts at: 186 186
Size: 4997
PstI C_TGCA'G
Cuts at: 2044 2729 2044
Size: 685 4312
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4997
PvuII CAG'CTG
Cuts at: 2422 2782 2422
Size: 360 4637
RsrII CG'GwC_CG
Cuts at: 3192 3192
Size: 4997
SacI G_AGCT'C
Cuts at: 2011 2011
Size: 4997
SalI G'TCGA_C
Cuts at: 2034 2034
Size: 4997
SapI GCTCTTCn'nnn_
Cuts at: 3020 3230 3020
Size: 210 4787
SbfI CC_TGCA'GG
Cuts at: 2044 2044
Size: 4997
ScaI AGT'ACT
Cuts at: 4514 4514
Size: 4997
SfcI C'TryA_G
Cuts at: 33 192 648 1700 1868 2040 2725 3718 3909
Size: 159 456 1052 168 172 685 993 191
Cuts at: 3909 33
Size: 1121
Fragments arranged by size:
1121 1052 993 685 456 191 172 168
159
SfoI GGC'GCC
Cuts at: 2429 2677 2429
Size: 248 4749
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4997
SmaI CCC'GGG
Cuts at: 2019 2019
Size: 4997
SmlI C'TyrA_G
Cuts at: 583 828 1842 2152 3067 3559 3821 583
Size: 245 1014 310 915 492 262 1759
Fragments arranged by size:
1759 1014 915 492 310 262 245
SpeI A'CTAG_T
Cuts at: 67 838 67
Size: 771 4226
SphI G_CATG'C
Cuts at: 2050 2550 2050
Size: 500 4497
StuI AGG'CCT
Cuts at: 646 1066 646
Size: 420 4577
StyI C'CwwG_G
Cuts at: 107 305 2131 2485 107
Size: 198 1826 354 2619
Fragments arranged by size:
2619 1826 354 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2580 2713 3172 3381 2580
Size: 133 459 209 4196
Fragments arranged by size:
4196 459 209 133
TatI w'GTAC_w
Cuts at: 503 4512 503
Size: 4009 988
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1263 1295 1318 1350 4514 4546 1263
Size: 32 23 32 3164 32 1714
Fragments arranged by size:
3164 1714 32 32 32 23
Tth111I GACn'n_nGTC
Cuts at: 2794 2794
Size: 4997
XbaI T'CTAG_A
Cuts at: 2028 2028
Size: 4997
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2492 114
Size: 2378 2619
XmaI C'CCGG_G
Cuts at: 2017 2017
Size: 4997
XmnI GAAnn'nnTTC
Cuts at: 339 898 1150 1187 1234 1318 1402 1486 1570
Size: 559 252 37 47 84 84 84 84
Cuts at: 1570 1654 1738 1822 339
Size: 84 84 84 3514
Fragments arranged by size:
3514 559 252 84 84 84 84 84
84 84 47 37
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BglI BmgBI BmtI BsmBI
BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV
FseI FspAI HpaI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI