Restriction map for
pF1KB8344
(Circular) (Six-base) MAPSORT of: pF1KB8344.seq Check: 7934 from: 1 to: 4016
pF1KB8344 4016 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1054 1054
Size: 4016
Acc65I G'GTAC_C
Cuts at: 1032 1032
Size: 4016
AclI AA'CG_TT
Cuts at: 3856 3856
Size: 4016
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 1841 2273 3020 3288 490
Size: 1351 432 747 268 1218
Fragments arranged by size:
1351 1218 747 432 268
AflIII A'CryG_T
Cuts at: 976 1368 2472 3449 976
Size: 392 1104 977 1543
Fragments arranged by size:
1543 1104 977 392
AgeI A'CCGG_T
Cuts at: 2404 2404
Size: 4016
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 247 247
Size: 4016
AlwNI CAG_nnn'CTG
Cuts at: 244 1321 2888 244
Size: 1077 1567 1372
Fragments arranged by size:
1567 1372 1077
ApaI G_GGCC'C
Cuts at: 575 575
Size: 4016
ApaLI G'TGCA_C
Cuts at: 2786 2786
Size: 4016
ApoI r'AATT_y
Cuts at: 916 1020 3369 3625 916
Size: 104 2349 256 1307
Fragments arranged by size:
2349 1307 256 104
AseI AT'TA_AT
Cuts at: 20 20
Size: 4016
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
AvaI C'yCGr_G
Cuts at: 1036 1036
Size: 4016
BamHI G'GATC_C
Cuts at: 1041 1041
Size: 4016
BanI G'GyrC_C
Cuts at: 305 775 1032 1446 1694 1729 305
Size: 470 257 414 248 35 2592
Fragments arranged by size:
2592 470 414 257 248 35
BanII G_rGCy'C
Cuts at: 575 766 1030 2060 575
Size: 191 264 1030 2531
Fragments arranged by size:
2531 1030 264 191
BbeI G_GCGC'C
Cuts at: 309 779 1450 1698 309
Size: 470 671 248 2627
Fragments arranged by size:
2627 671 470 248
BciVI GTATCCnnnnn_n'
Cuts at: 1914 2675 4016 1914
Size: 761 1341 1914
Fragments arranged by size:
1914 1341 761
BclI T'GATC_A
Cuts at: 293 368 1536 293
Size: 75 1168 2773
Fragments arranged by size:
2773 1168 75
BglII A'GATC_T
Cuts at: 596 1531 3568 596
Size: 935 2037 1044
Fragments arranged by size:
2037 1044 935
BlpI GC'TnA_GC
Cuts at: 245 1128 245
Size: 883 3133
Bme1580I G_kGCm'C
Cuts at: 216 575 1641 1734 2790 216
Size: 359 1066 93 1056 1442
Fragments arranged by size:
1442 1066 1056 359 93
BmrI ACTGGGnnnn_n'
Cuts at: 1228 1309 1644 3788 1228
Size: 81 335 2144 1456
Fragments arranged by size:
2144 1456 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 4016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 585 895 424
Size: 161 310 3545
Fragments arranged by size:
3545 310 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1192 2071 2563 2861 1192
Size: 879 492 298 2347
Fragments arranged by size:
2347 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3720 3720
Size: 4016
BsaAI yAC'GTr
Cuts at: 977 1369 977
Size: 392 3624
BsaBI GATnn'nnATC
Cuts at: 6 1530 3567 6
Size: 1524 2037 455
Fragments arranged by size:
2037 1524 455
BsaHI Gr'CG_yC
Cuts at: 306 776 1447 1695 2397 3893 306
Size: 470 671 248 702 1496 429
Fragments arranged by size:
1496 702 671 470 429 248
BsaWI w'CCGG_w
Cuts at: 527 1205 1726 2404 2678 2825 3236 527
Size: 678 521 678 274 147 411 1307
Fragments arranged by size:
1307 678 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 331 361 331
Size: 30 3986
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 355 427 430 355
Size: 72 3 3941
Fragments arranged by size:
3941 72 3
BseYI C'CCAG_C
Cuts at: 132 216 735 870 1441 2199 2776 3272 132
Size: 84 519 135 571 758 577 496 876
Fragments arranged by size:
876 758 577 571 519 496 135 84
BsiEI CG_ry'CG
Cuts at: 104 1558 1604 2812 104
Size: 1454 46 1208 1308
Fragments arranged by size:
1454 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 766 1030 1808 1998 2790 766
Size: 264 778 190 792 1992
Fragments arranged by size:
1992 792 778 264 190
BsmI GAATG_Cn'
Cuts at: 680 804 680
Size: 124 3892
Bsp1286I G_dGCh'C
Cuts at: 216 575 766 1030 1641 1734 1808 1998 2060
Size: 359 191 264 611 93 74 190 62
Cuts at: 2060 2790 216
Size: 730 1442
Fragments arranged by size:
1442 730 611 359 264 191 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3236 3236
Size: 4016
BspHI T'CATG_A
Cuts at: 3192 3192
Size: 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 383 576 1066 1280 1582 1963 383
Size: 193 490 214 302 381 2436
Fragments arranged by size:
2436 490 381 302 214 193
BsrBI CCG'CTC
Cuts at: 50 2308 2362 3846 4011 50
Size: 2258 54 1484 165 55
Fragments arranged by size:
2258 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1928 1928
Size: 4016
BsrFI r'CCGG_y
Cuts at: 2014 2195 2404 2014
Size: 181 209 3626
Fragments arranged by size:
3626 209 181
BssSI C'ACGA_G
Cuts at: 187 2287 2645 187
Size: 2100 358 1558
Fragments arranged by size:
2100 1558 358
BstAPI GCAn_nnn'nTGC
Cuts at: 716 1393 716
Size: 677 3339
BstBI TT'CG_AA
Cuts at: 2377 2377
Size: 4016
BstXI CCAn_nnnn'nTGG
Cuts at: 919 919
Size: 4016
BstYI r'GATC_y
Cuts at: 1 596 1041 1508 1531 1866 2112 3124 3210
Size: 595 445 467 23 335 246 1012 86
Cuts at: 3210 3222 3568 1
Size: 12 346 449
Fragments arranged by size:
1012 595 467 449 445 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1966 2147 2310 1966
Size: 181 163 3672
Fragments arranged by size:
3672 181 163
BtsI GCAGTG_nn'
Cuts at: 171 352 1259 171
Size: 181 907 2928
Fragments arranged by size:
2928 907 181
DraI TTT'AAA
Cuts at: 1015 1015
Size: 4016
DrdI GACnn_nn'nnGTC
Cuts at: 393 1722 2580 393
Size: 1329 858 1829
Fragments arranged by size:
1829 1329 858
EaeI y'GGCC_r
Cuts at: 1601 1775 2166 2193 3538 1601
Size: 174 391 27 1345 2079
Fragments arranged by size:
2079 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1601 1601
Size: 4016
EarI CTCTTCn'nnn_
Cuts at: 404 503 539 2039 2249 404
Size: 99 36 1500 210 2171
Fragments arranged by size:
2171 1500 210 99 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2224 2534 2680 3826 2224
Size: 310 146 1146 2414
Fragments arranged by size:
2414 1146 310 146
EcoICRI GAG'CTC
Cuts at: 764 1028 764
Size: 264 3752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 490 585 895 1841 2273 3020 3288 424
Size: 66 95 310 946 432 747 268 1152
Fragments arranged by size:
1152 946 747 432 310 268 95 66
EcoO109I rG'GnC_Cy
Cuts at: 395 571 572 1155 395
Size: 176 1 583 3256
Fragments arranged by size:
3256 583 176 1
EcoRI G'AATT_C
Cuts at: 1020 1020
Size: 4016
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 960 2035 960
Size: 1075 2941
FspI TGC'GCA
Cuts at: 1797 1797
Size: 4016
HaeII r_GCGC'y
Cuts at: 309 779 1450 1698 2720 3699 309
Size: 470 671 248 1022 979 626
Fragments arranged by size:
1022 979 671 626 470 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1858 1888 1890 1920 1858
Size: 30 2 30 3954
Fragments arranged by size:
3954 30 30 2
HincII GTy'rAC
Cuts at: 273 1055 273
Size: 782 3234
HindIII A'AGCT_T
Cuts at: 902 902
Size: 4016
HpaI GTT'AAC
Cuts at: 273 273
Size: 4016
KasI G'GCGC_C
Cuts at: 305 775 1446 1694 305
Size: 470 671 248 2627
Fragments arranged by size:
2627 671 470 248
KpnI G_GTAC'C
Cuts at: 1036 1036
Size: 4016
MluI A'CGCG_T
Cuts at: 3449 3449
Size: 4016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 302 2687 2871 302
Size: 2385 184 1447
Fragments arranged by size:
2385 1447 184
MscI TGG'CCA
Cuts at: 1777 1777
Size: 4016
MslI CAynn'nnrTG
Cuts at: 404 668 2005 2132 404
Size: 264 1337 127 2288
Fragments arranged by size:
2288 1337 264 127
MspA1I CmG'CkG
Cuts at: 739 870 1127 1441 1801 2814 3059 739
Size: 131 257 314 360 1013 245 1696
Fragments arranged by size:
1696 1013 360 314 257 245 131
NaeI GCC'GGC
Cuts at: 2197 2197
Size: 4016
NarI GG'CG_CC
Cuts at: 306 776 1447 1695 306
Size: 470 671 248 2627
Fragments arranged by size:
2627 671 470 248
NgoMIV G'CCGG_C
Cuts at: 2195 2195
Size: 4016
NspI r_CATG'y
Cuts at: 1069 1569 2476 1069
Size: 500 907 2609
Fragments arranged by size:
2609 907 500
PciI A'CATG_T
Cuts at: 2472 2472
Size: 4016
PfoI T'CCnGG_A
Cuts at: 440 911 440
Size: 471 3545
PmeI GTTT'AAAC
Cuts at: 1015 1015
Size: 4016
PmlI CAC'GTG
Cuts at: 977 977
Size: 4016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3155 3187 3155
Size: 32 3984
PpuMI rG'GwC_Cy
Cuts at: 395 395
Size: 4016
PspOMI G'GGCC_C
Cuts at: 571 571
Size: 4016
PstI C_TGCA'G
Cuts at: 149 240 521 1063 1748 149
Size: 91 281 542 685 2417
Fragments arranged by size:
2417 685 542 281 91
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4016
PvuII CAG'CTG
Cuts at: 739 870 1441 1801 739
Size: 131 571 360 2954
Fragments arranged by size:
2954 571 360 131
RsrII CG'GwC_CG
Cuts at: 2211 2211
Size: 4016
SacI G_AGCT'C
Cuts at: 766 1030 766
Size: 264 3752
SalI G'TCGA_C
Cuts at: 1053 1053
Size: 4016
SapI GCTCTTCn'nnn_
Cuts at: 2039 2249 2039
Size: 210 3806
SbfI CC_TGCA'GG
Cuts at: 1063 1063
Size: 4016
ScaI AGT'ACT
Cuts at: 151 3533 151
Size: 3382 634
SfcI C'TryA_G
Cuts at: 33 145 156 236 517 1059 1744 2737 2928
Size: 112 11 80 281 542 685 993 191
Cuts at: 2928 33
Size: 1121
Fragments arranged by size:
1121 993 685 542 281 191 112 80
11
SfoI GGC'GCC
Cuts at: 307 777 1448 1696 307
Size: 470 671 248 2627
Fragments arranged by size:
2627 671 470 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
SmaI CCC'GGG
Cuts at: 1038 1038
Size: 4016
SmlI C'TyrA_G
Cuts at: 1171 2086 2578 2840 1171
Size: 915 492 262 2347
Fragments arranged by size:
2347 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4016
SphI G_CATG'C
Cuts at: 1069 1569 1069
Size: 500 3516
StyI C'CwwG_G
Cuts at: 1150 1504 1150
Size: 354 3662
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 215 706 1599 1732 2191 2400 215
Size: 491 893 133 459 209 1831
Fragments arranged by size:
1831 893 491 459 209 133
TatI w'GTAC_w
Cuts at: 149 3531 149
Size: 3382 634
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 331 363 3533 3565 331
Size: 32 3170 32 782
Fragments arranged by size:
3170 782 32 32
Tth111I GACn'n_nGTC
Cuts at: 1813 1813
Size: 4016
XbaI T'CTAG_A
Cuts at: 1047 1047
Size: 4016
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1511 1511
Size: 4016
XmaI C'CCGG_G
Cuts at: 1036 1036
Size: 4016
XmnI GAAnn'nnTTC
Cuts at: 512 905 512
Size: 393 3623
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI