Restriction map for
pF1KB8342
(Circular) (Six-base) MAPSORT of: pF1KB8342.seq Check: 8678 from: 1 to: 4157
pF1KB8342 4157 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1195 1195
Size: 4157
Acc65I G'GTAC_C
Cuts at: 1173 1173
Size: 4157
AclI AA'CG_TT
Cuts at: 3997 3997
Size: 4157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1982 2414 3161 3429 1982
Size: 432 747 268 2710
Fragments arranged by size:
2710 747 432 268
AflIII A'CryG_T
Cuts at: 236 725 1509 2613 3590 236
Size: 489 784 1104 977 803
Fragments arranged by size:
1104 977 803 784 489
AgeI A'CCGG_T
Cuts at: 2545 2545
Size: 4157
AlwNI CAG_nnn'CTG
Cuts at: 1462 3029 1462
Size: 1567 2590
ApaI G_GGCC'C
Cuts at: 428 428
Size: 4157
ApaLI G'TGCA_C
Cuts at: 2927 2927
Size: 4157
ApoI r'AATT_y
Cuts at: 1161 3510 3766 1161
Size: 2349 256 1552
Fragments arranged by size:
2349 1552 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4157
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4157
AvaI C'yCGr_G
Cuts at: 646 1177 646
Size: 531 3626
AvrII C'CTAG_G
Cuts at: 369 369
Size: 4157
BamHI G'GATC_C
Cuts at: 1182 1182
Size: 4157
BanI G'GyrC_C
Cuts at: 903 1173 1587 1835 1870 903
Size: 270 414 248 35 3190
Fragments arranged by size:
3190 414 270 248 35
BanII G_rGCy'C
Cuts at: 428 949 1171 2201 428
Size: 521 222 1030 2384
Fragments arranged by size:
2384 1030 521 222
BbeI G_GCGC'C
Cuts at: 1591 1839 1591
Size: 248 3909
BbsI GAAGACnn'nnnn_
Cuts at: 411 780 411
Size: 369 3788
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 159 193 159
Size: 34 4123
BciVI GTATCCnnnnn_n'
Cuts at: 403 2055 2816 4157 403
Size: 1652 761 1341 403
Fragments arranged by size:
1652 1341 761 403
BclI T'GATC_A
Cuts at: 1677 1677
Size: 4157
BglI GCCn_nnn'nGGC
Cuts at: 919 919
Size: 4157
BglII A'GATC_T
Cuts at: 1672 3709 1672
Size: 2037 2120
BlpI GC'TnA_GC
Cuts at: 203 1269 203
Size: 1066 3091
Bme1580I G_kGCm'C
Cuts at: 143 428 908 1782 1875 2931 143
Size: 285 480 874 93 1056 1369
Fragments arranged by size:
1369 1056 874 480 285 93
BmrI ACTGGGnnnn_n'
Cuts at: 1099 1369 1450 1785 3929 1099
Size: 270 81 335 2144 1327
Fragments arranged by size:
2144 1327 335 270 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2524 2524
Size: 4157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 1333 2212 2704 3002 673
Size: 660 879 492 298 1828
Fragments arranged by size:
1828 879 660 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3861 3861
Size: 4157
BsaAI yAC'GTr
Cuts at: 138 516 1510 138
Size: 378 994 2785
Fragments arranged by size:
2785 994 378
BsaBI GATnn'nnATC
Cuts at: 6 760 1671 3708 6
Size: 754 911 2037 455
Fragments arranged by size:
2037 911 754 455
BsaHI Gr'CG_yC
Cuts at: 1588 1836 2538 4034 1588
Size: 248 702 1496 1711
Fragments arranged by size:
1711 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1046 1346 1867 2545 2819 2966 3377 1046
Size: 300 521 678 274 147 411 1826
Fragments arranged by size:
1826 678 521 411 300 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 931 961 931
Size: 30 4127
BseYI C'CCAG_C
Cuts at: 255 812 899 978 1582 2340 2917 3413 255
Size: 557 87 79 604 758 577 496 999
Fragments arranged by size:
999 758 604 577 557 496 87 79
BsiEI CG_ry'CG
Cuts at: 104 1151 1699 1745 2953 104
Size: 1047 548 46 1208 1308
Fragments arranged by size:
1308 1208 1047 548 46
BsiHKAI G_wGCw'C
Cuts at: 664 1171 1949 2139 2931 664
Size: 507 778 190 792 1890
Fragments arranged by size:
1890 792 778 507 190
BsmI GAATG_Cn'
Cuts at: 922 922
Size: 4157
BsmBI CGTCTCn'nnnn_
Cuts at: 1123 1123
Size: 4157
Bsp1286I G_dGCh'C
Cuts at: 143 428 664 908 949 1171 1782 1875 1949
Size: 285 236 244 41 222 611 93 74
Cuts at: 1949 2139 2201 2931 143
Size: 190 62 730 1369
Fragments arranged by size:
1369 730 611 285 244 236 222 190
93 74 62 41
BspEI T'CCGG_A
Cuts at: 1046 3377 1046
Size: 2331 1826
BspHI T'CATG_A
Cuts at: 968 1028 3333 968
Size: 60 2305 1792
Fragments arranged by size:
2305 1792 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1207 1421 1723 2104 1207
Size: 214 302 381 3260
Fragments arranged by size:
3260 381 302 214
BsrBI CCG'CTC
Cuts at: 50 510 2449 2503 3987 4152 50
Size: 460 1939 54 1484 165 55
Fragments arranged by size:
1939 1484 460 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2069 2069
Size: 4157
BsrFI r'CCGG_y
Cuts at: 625 2155 2336 2545 625
Size: 1530 181 209 2237
Fragments arranged by size:
2237 1530 209 181
BssSI C'ACGA_G
Cuts at: 663 2428 2786 663
Size: 1765 358 2034
Fragments arranged by size:
2034 1765 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1534 1534
Size: 4157
BstBI TT'CG_AA
Cuts at: 2518 2518
Size: 4157
BstEII G'GTnAC_C
Cuts at: 855 855
Size: 4157
BstYI r'GATC_y
Cuts at: 1 1182 1649 1672 2007 2253 3265 3351 3363
Size: 1181 467 23 335 246 1012 86 12
Cuts at: 3363 3709 1
Size: 346 449
Fragments arranged by size:
1181 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 208 347 208
Size: 139 4018
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 867 2107 2288 2451 867
Size: 1240 181 163 2573
Fragments arranged by size:
2573 1240 181 163
BtsI GCAGTG_nn'
Cuts at: 664 1400 664
Size: 736 3421
DraI TTT'AAA
Cuts at: 1156 1156
Size: 4157
DraIII CAC_nnn'GTG
Cuts at: 1093 1093
Size: 4157
DrdI GACnn_nn'nnGTC
Cuts at: 1863 2721 1863
Size: 858 3299
EaeI y'GGCC_r
Cuts at: 227 500 1148 1742 1916 2307 2334 3679 227
Size: 273 648 594 174 391 27 1345 705
Fragments arranged by size:
1345 705 648 594 391 273 174 27
EagI C'GGCC_G
Cuts at: 1148 1742 1148
Size: 594 3563
EarI CTCTTCn'nnn_
Cuts at: 364 2180 2390 364
Size: 1816 210 2131
Fragments arranged by size:
2131 1816 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2365 2675 2821 3967 2365
Size: 310 146 1146 2555
Fragments arranged by size:
2555 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1169 1169
Size: 4157
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1982 2414 3161 3429 1982
Size: 432 747 268 2710
Fragments arranged by size:
2710 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 124 651 1296 124
Size: 527 645 2985
Fragments arranged by size:
2985 645 527
EcoRI G'AATT_C
Cuts at: 1161 1161
Size: 4157
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 4157
FspI TGC'GCA
Cuts at: 313 1938 313
Size: 1625 2532
HaeII r_GCGC'y
Cuts at: 601 1001 1591 1839 2861 3840 601
Size: 400 590 248 1022 979 918
Fragments arranged by size:
1022 979 918 590 400 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 401 433 1090 1122 1999 2029 2031 2061 401
Size: 32 657 32 877 30 2 30 2497
Fragments arranged by size:
2497 877 657 32 32 30 30 2
HincII GTy'rAC
Cuts at: 702 1196 702
Size: 494 3663
KasI G'GCGC_C
Cuts at: 1587 1835 1587
Size: 248 3909
KpnI G_GTAC'C
Cuts at: 1177 1177
Size: 4157
MluI A'CGCG_T
Cuts at: 3590 3590
Size: 4157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 974 2828 3012 974
Size: 1854 184 2119
Fragments arranged by size:
2119 1854 184
MscI TGG'CCA
Cuts at: 502 1918 502
Size: 1416 2741
MslI CAynn'nnrTG
Cuts at: 724 730 751 862 874 967 2146 2273 724
Size: 6 21 111 12 93 1179 127 2608
Fragments arranged by size:
2608 1179 127 111 93 21 12 6
MspA1I CmG'CkG
Cuts at: 808 1268 1582 1942 2955 3200 808
Size: 460 314 360 1013 245 1765
Fragments arranged by size:
1765 1013 460 360 314 245
NaeI GCC'GGC
Cuts at: 627 2338 627
Size: 1711 2446
NarI GG'CG_CC
Cuts at: 1588 1836 1588
Size: 248 3909
NcoI C'CATG_G
Cuts at: 208 208
Size: 4157
NgoMIV G'CCGG_C
Cuts at: 625 2336 625
Size: 1711 2446
NspI r_CATG'y
Cuts at: 240 729 1059 1210 1710 2617 240
Size: 489 330 151 500 907 1780
Fragments arranged by size:
1780 907 500 489 330 151
PasI CC'CwG_GG
Cuts at: 462 462
Size: 4157
PciI A'CATG_T
Cuts at: 236 725 2613 236
Size: 489 1888 1780
Fragments arranged by size:
1888 1780 489
PmeI GTTT'AAAC
Cuts at: 1156 1156
Size: 4157
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3296 3328 3296
Size: 32 4125
PspOMI G'GGCC_C
Cuts at: 424 424
Size: 4157
PstI C_TGCA'G
Cuts at: 659 1204 1889 659
Size: 545 685 2927
Fragments arranged by size:
2927 685 545
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4157
PvuII CAG'CTG
Cuts at: 1582 1942 1582
Size: 360 3797
RsrII CG'GwC_CG
Cuts at: 2352 2352
Size: 4157
SacI G_AGCT'C
Cuts at: 1171 1171
Size: 4157
SalI G'TCGA_C
Cuts at: 1194 1194
Size: 4157
SapI GCTCTTCn'nnn_
Cuts at: 2180 2390 2180
Size: 210 3947
SbfI CC_TGCA'GG
Cuts at: 1204 1204
Size: 4157
ScaI AGT'ACT
Cuts at: 193 3674 193
Size: 3481 676
SfcI C'TryA_G
Cuts at: 33 655 1200 1885 2878 3069 33
Size: 622 545 685 993 191 1121
Fragments arranged by size:
1121 993 685 622 545 191
SfoI GGC'GCC
Cuts at: 1589 1837 1589
Size: 248 3909
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4157
SmaI CCC'GGG
Cuts at: 1179 1179
Size: 4157
SmlI C'TyrA_G
Cuts at: 688 1312 2227 2719 2981 688
Size: 624 915 492 262 1864
Fragments arranged by size:
1864 915 624 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4157
SphI G_CATG'C
Cuts at: 1059 1210 1710 1059
Size: 151 500 3506
Fragments arranged by size:
3506 500 151
StuI AGG'CCT
Cuts at: 1105 1105
Size: 4157
StyI C'CwwG_G
Cuts at: 208 369 488 1291 1645 208
Size: 161 119 803 354 2720
Fragments arranged by size:
2720 803 354 161 119
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 891 946 952 1081 1740 1873 2332 2541 891
Size: 55 6 129 659 133 459 209 2507
Fragments arranged by size:
2507 659 459 209 133 129 55 6
TatI w'GTAC_w
Cuts at: 191 3672 191
Size: 3481 676
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 931 963 3674 3706 931
Size: 32 2711 32 1382
Fragments arranged by size:
2711 1382 32 32
Tth111I GACn'n_nGTC
Cuts at: 1954 1954
Size: 4157
XbaI T'CTAG_A
Cuts at: 1188 1188
Size: 4157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 626 1652 626
Size: 1026 3131
XmaI C'CCGG_G
Cuts at: 1177 1177
Size: 4157
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BbvCI BfrBI BmgBI BmtI BpmI Bpu10I
BseRI BsgI BsiWI BsrGI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI