Restriction map for
pF1KB8339
(Circular) (Six-base) MAPSORT of: pF1KB8339.seq Check: 4311 from: 1 to: 4043
pF1KB8339 4043 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1081 1081
Size: 4043
Acc65I G'GTAC_C
Cuts at: 496 1059 496
Size: 563 3480
AclI AA'CG_TT
Cuts at: 1037 3883 1037
Size: 2846 1197
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 914 1868 2300 3047 3315 914
Size: 954 432 747 268 1642
Fragments arranged by size:
1642 954 747 432 268
AfeI AGC'GCT
Cuts at: 634 634
Size: 4043
AflIII A'CryG_T
Cuts at: 1395 2499 3476 1395
Size: 1104 977 1962
Fragments arranged by size:
1962 1104 977
AgeI A'CCGG_T
Cuts at: 2431 2431
Size: 4043
AleI CACnn'nnGTG
Cuts at: 475 475
Size: 4043
AlwNI CAG_nnn'CTG
Cuts at: 442 520 1348 2915 442
Size: 78 828 1567 1570
Fragments arranged by size:
1570 1567 828 78
ApaLI G'TGCA_C
Cuts at: 2813 2813
Size: 4043
ApoI r'AATT_y
Cuts at: 1047 3396 3652 1047
Size: 2349 256 1438
Fragments arranged by size:
2349 1438 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4043
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4043
AvaI C'yCGr_G
Cuts at: 692 1063 692
Size: 371 3672
AvrII C'CTAG_G
Cuts at: 646 646
Size: 4043
BamHI G'GATC_C
Cuts at: 1068 1068
Size: 4043
BanI G'GyrC_C
Cuts at: 343 496 998 1059 1473 1721 1756 343
Size: 153 502 61 414 248 35 2630
Fragments arranged by size:
2630 502 414 248 153 61 35
BanII G_rGCy'C
Cuts at: 541 714 990 1057 2087 541
Size: 173 276 67 1030 2497
Fragments arranged by size:
2497 1030 276 173 67
BbeI G_GCGC'C
Cuts at: 347 1477 1725 347
Size: 1130 248 2665
Fragments arranged by size:
2665 1130 248
BbsI GAAGACnn'nnnn_
Cuts at: 191 249 191
Size: 58 3985
BciVI GTATCCnnnnn_n'
Cuts at: 1941 2702 4043 1941
Size: 761 1341 1941
Fragments arranged by size:
1941 1341 761
BclI T'GATC_A
Cuts at: 1563 1563
Size: 4043
BglII A'GATC_T
Cuts at: 957 1558 3595 957
Size: 601 2037 1405
Fragments arranged by size:
2037 1405 601
BlpI GC'TnA_GC
Cuts at: 713 1155 713
Size: 442 3601
Bme1580I G_kGCm'C
Cuts at: 1668 1761 2817 1668
Size: 93 1056 2894
Fragments arranged by size:
2894 1056 93
BmgBI CAC'GTC
Cuts at: 936 936
Size: 4043
BmrI ACTGGGnnnn_n'
Cuts at: 188 1255 1336 1671 3815 188
Size: 1067 81 335 2144 416
Fragments arranged by size:
2144 1067 416 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 4043
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1219 2098 2590 2888 1219
Size: 879 492 298 2374
Fragments arranged by size:
2374 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3747 3747
Size: 4043
BsaAI yAC'GTr
Cuts at: 1396 1396
Size: 4043
BsaBI GATnn'nnATC
Cuts at: 6 1557 3594 6
Size: 1551 2037 455
Fragments arranged by size:
2037 1551 455
BsaHI Gr'CG_yC
Cuts at: 344 1474 1722 2424 3920 344
Size: 1130 248 702 1496 467
Fragments arranged by size:
1496 1130 702 467 248
BsaWI w'CCGG_w
Cuts at: 1232 1753 2431 2705 2852 3263 1232
Size: 521 678 274 147 411 2012
Fragments arranged by size:
2012 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 466 552 1468 2226 2803 3299 466
Size: 86 916 758 577 496 1210
Fragments arranged by size:
1210 916 758 577 496 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 188 787 172
Size: 16 599 3428
Fragments arranged by size:
3428 599 16
BsiEI CG_ry'CG
Cuts at: 104 1585 1631 2839 104
Size: 1481 46 1208 1308
Fragments arranged by size:
1481 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 808 990 1057 1835 2025 2817 808
Size: 182 67 778 190 792 2034
Fragments arranged by size:
2034 792 778 190 182 67
BsmBI CGTCTCn'nnnn_
Cuts at: 439 439
Size: 4043
Bsp1286I G_dGCh'C
Cuts at: 541 714 808 990 1057 1668 1761 1835 2025
Size: 173 94 182 67 611 93 74 190
Cuts at: 2025 2087 2817 541
Size: 62 730 1767
Fragments arranged by size:
1767 730 611 190 182 173 94 93
74 67 62
BspEI T'CCGG_A
Cuts at: 3263 3263
Size: 4043
BspHI T'CATG_A
Cuts at: 3219 3219
Size: 4043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1093 1307 1609 1990 1093
Size: 214 302 381 3146
Fragments arranged by size:
3146 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2335 2389 3873 4038 50
Size: 2285 54 1484 165 55
Fragments arranged by size:
2285 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1955 1955
Size: 4043
BsrFI r'CCGG_y
Cuts at: 2041 2222 2431 2041
Size: 181 209 3653
Fragments arranged by size:
3653 209 181
BsrGI T'GTAC_A
Cuts at: 287 287
Size: 4043
BssSI C'ACGA_G
Cuts at: 2314 2672 2314
Size: 358 3685
BstAPI GCAn_nnn'nTGC
Cuts at: 1420 1420
Size: 4043
BstBI TT'CG_AA
Cuts at: 2404 2404
Size: 4043
BstYI r'GATC_y
Cuts at: 1 957 1068 1535 1558 1893 2139 3151 3237
Size: 956 111 467 23 335 246 1012 86
Cuts at: 3237 3249 3595 1
Size: 12 346 449
Fragments arranged by size:
1012 956 467 449 346 335 246 111
86 23 12
BtgI C'CryG_G
Cuts at: 1002 1002
Size: 4043
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1993 2174 2337 1993
Size: 181 163 3699
Fragments arranged by size:
3699 181 163
BtsI GCAGTG_nn'
Cuts at: 176 482 547 1286 176
Size: 306 65 739 2933
Fragments arranged by size:
2933 739 306 65
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 179 214 179
Size: 35 4008
DraI TTT'AAA
Cuts at: 1042 1042
Size: 4043
DrdI GACnn_nn'nnGTC
Cuts at: 244 1749 2607 244
Size: 1505 858 1680
Fragments arranged by size:
1680 1505 858
EaeI y'GGCC_r
Cuts at: 324 816 1628 1802 2193 2220 3565 324
Size: 492 812 174 391 27 1345 802
Fragments arranged by size:
1345 812 802 492 391 174 27
EagI C'GGCC_G
Cuts at: 1628 1628
Size: 4043
EarI CTCTTCn'nnn_
Cuts at: 134 2066 2276 134
Size: 1932 210 1901
Fragments arranged by size:
1932 1901 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2251 2561 2707 3853 2251
Size: 310 146 1146 2441
Fragments arranged by size:
2441 1146 310 146
EcoICRI GAG'CTC
Cuts at: 988 1055 988
Size: 67 3976
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 914 1868 2300 3047 3315 914
Size: 954 432 747 268 1642
Fragments arranged by size:
1642 954 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1182 1182
Size: 4043
EcoRI G'AATT_C
Cuts at: 1047 1047
Size: 4043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 273 2062 273
Size: 1789 2254
FspI TGC'GCA
Cuts at: 1824 1824
Size: 4043
HaeII r_GCGC'y
Cuts at: 347 636 1477 1725 2747 3726 347
Size: 289 841 248 1022 979 664
Fragments arranged by size:
1022 979 841 664 289 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 734 766 998 1030 1885 1915 1917 1947 734
Size: 32 232 32 855 30 2 30 2830
Fragments arranged by size:
2830 855 232 32 32 30 30 2
HincII GTy'rAC
Cuts at: 423 1082 423
Size: 659 3384
HindIII A'AGCT_T
Cuts at: 845 845
Size: 4043
KasI G'GCGC_C
Cuts at: 343 1473 1721 343
Size: 1130 248 2665
Fragments arranged by size:
2665 1130 248
KpnI G_GTAC'C
Cuts at: 500 1063 500
Size: 563 3480
MfeI C'AATT_G
Cuts at: 638 638
Size: 4043
MluI A'CGCG_T
Cuts at: 3476 3476
Size: 4043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2714 2898 2714
Size: 184 3859
MscI TGG'CCA
Cuts at: 326 818 1804 326
Size: 492 986 2565
Fragments arranged by size:
2565 986 492
MslI CAynn'nnrTG
Cuts at: 475 2032 2159 475
Size: 1557 127 2359
Fragments arranged by size:
2359 1557 127
MspA1I CmG'CkG
Cuts at: 130 223 318 439 517 924 1154 1468 1828
Size: 93 95 121 78 407 230 314 360
Cuts at: 1828 2841 3086 130
Size: 1013 245 1087
Fragments arranged by size:
1087 1013 407 360 314 245 230 121
95 93 78
NaeI GCC'GGC
Cuts at: 2224 2224
Size: 4043
NarI GG'CG_CC
Cuts at: 344 1474 1722 344
Size: 1130 248 2665
Fragments arranged by size:
2665 1130 248
NgoMIV G'CCGG_C
Cuts at: 2222 2222
Size: 4043
NspI r_CATG'y
Cuts at: 1096 1596 2503 1096
Size: 500 907 2636
Fragments arranged by size:
2636 907 500
PasI CC'CwG_GG
Cuts at: 534 534
Size: 4043
PciI A'CATG_T
Cuts at: 2499 2499
Size: 4043
PfoI T'CCnGG_A
Cuts at: 860 860
Size: 4043
PmeI GTTT'AAAC
Cuts at: 1042 1042
Size: 4043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3182 3214 3182
Size: 32 4011
PstI C_TGCA'G
Cuts at: 620 905 923 1090 1775 620
Size: 285 18 167 685 2888
Fragments arranged by size:
2888 685 285 167 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4043
PvuII CAG'CTG
Cuts at: 130 223 318 439 517 924 1468 1828 130
Size: 93 95 121 78 407 544 360 2345
Fragments arranged by size:
2345 544 407 360 121 95 93 78
RsrII CG'GwC_CG
Cuts at: 2238 2238
Size: 4043
SacI G_AGCT'C
Cuts at: 990 1057 990
Size: 67 3976
SalI G'TCGA_C
Cuts at: 1080 1080
Size: 4043
SapI GCTCTTCn'nnn_
Cuts at: 2066 2276 2066
Size: 210 3833
SbfI CC_TGCA'GG
Cuts at: 1090 1090
Size: 4043
ScaI AGT'ACT
Cuts at: 614 3560 614
Size: 2946 1097
SfcI C'TryA_G
Cuts at: 33 616 901 919 1086 1771 2764 2955 33
Size: 583 285 18 167 685 993 191 1121
Fragments arranged by size:
1121 993 685 583 285 191 167 18
SfoI GGC'GCC
Cuts at: 345 1475 1723 345
Size: 1130 248 2665
Fragments arranged by size:
2665 1130 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4043
SmaI CCC'GGG
Cuts at: 1065 1065
Size: 4043
SmlI C'TyrA_G
Cuts at: 1198 2113 2605 2867 1198
Size: 915 492 262 2374
Fragments arranged by size:
2374 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4043
SphI G_CATG'C
Cuts at: 1096 1596 1096
Size: 500 3543
StyI C'CwwG_G
Cuts at: 646 1177 1531 646
Size: 531 354 3158
Fragments arranged by size:
3158 531 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1626 1759 2218 2427 1626
Size: 133 459 209 3242
Fragments arranged by size:
3242 459 209 133
TatI w'GTAC_w
Cuts at: 287 511 612 3558 287
Size: 224 101 2946 772
Fragments arranged by size:
2946 772 224 101
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 646 678 3560 3592 646
Size: 32 2882 32 1097
Fragments arranged by size:
2882 1097 32 32
Tth111I GACn'n_nGTC
Cuts at: 1840 1840
Size: 4043
XbaI T'CTAG_A
Cuts at: 1074 1074
Size: 4043
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 474 826 1538 474
Size: 352 712 2979
Fragments arranged by size:
2979 712 352
XmaI C'CCGG_G
Cuts at: 1063 1063
Size: 4043
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
BaeI BbvCI BcgI BfrBI BglI BmtI BpmI Bpu10I
BsaXI BseRI BsiWI BsmI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HpaI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI