Restriction map for
pF1KB8338
(Circular) (Six-base) MAPSORT of: pF1KB8338.seq Check: 5423 from: 1 to: 3929
pF1KB8338 3929 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 967 967
Size: 3929
Acc65I G'GTAC_C
Cuts at: 945 945
Size: 3929
AclI AA'CG_TT
Cuts at: 3769 3769
Size: 3929
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 1754 2186 2933 3201 243
Size: 1511 432 747 268 971
Fragments arranged by size:
1511 971 747 432 268
AflIII A'CryG_T
Cuts at: 791 1281 2385 3362 791
Size: 490 1104 977 1358
Fragments arranged by size:
1358 1104 977 490
AgeI A'CCGG_T
Cuts at: 2317 2317
Size: 3929
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 445 445
Size: 3929
AlwNI CAG_nnn'CTG
Cuts at: 762 1234 2801 762
Size: 472 1567 1890
Fragments arranged by size:
1890 1567 472
ApaI G_GGCC'C
Cuts at: 126 126
Size: 3929
ApaLI G'TGCA_C
Cuts at: 316 382 2699 316
Size: 66 2317 1546
Fragments arranged by size:
2317 1546 66
ApoI r'AATT_y
Cuts at: 933 3282 3538 933
Size: 2349 256 1324
Fragments arranged by size:
2349 1324 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3929
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
AvaI C'yCGr_G
Cuts at: 949 949
Size: 3929
BamHI G'GATC_C
Cuts at: 954 954
Size: 3929
BanI G'GyrC_C
Cuts at: 160 298 328 393 411 625 737 945 1359
Size: 138 30 65 18 214 112 208 414
Cuts at: 1359 1607 1642 160
Size: 248 35 2447
Fragments arranged by size:
2447 414 248 214 208 138 112 65
35 30 18
BanII G_rGCy'C
Cuts at: 126 449 943 1973 126
Size: 323 494 1030 2082
Fragments arranged by size:
2082 1030 494 323
BbeI G_GCGC'C
Cuts at: 164 302 332 397 415 629 1363 1611 164
Size: 138 30 65 18 214 734 248 2482
Fragments arranged by size:
2482 734 248 214 138 65 30 18
BciVI GTATCCnnnnn_n'
Cuts at: 1827 2588 3929 1827
Size: 761 1341 1827
Fragments arranged by size:
1827 1341 761
BclI T'GATC_A
Cuts at: 1449 1449
Size: 3929
BglI GCCn_nnn'nGGC
Cuts at: 225 225
Size: 3929
BglII A'GATC_T
Cuts at: 860 1444 3481 860
Size: 584 2037 1308
Fragments arranged by size:
2037 1308 584
BlpI GC'TnA_GC
Cuts at: 181 1041 181
Size: 860 3069
Bme1580I G_kGCm'C
Cuts at: 126 320 386 742 1554 1647 2703 126
Size: 194 66 356 812 93 1056 1352
Fragments arranged by size:
1352 1056 812 356 194 93 66
BmrI ACTGGGnnnn_n'
Cuts at: 128 307 1141 1222 1557 3701 128
Size: 179 834 81 335 2144 356
Fragments arranged by size:
2144 834 356 335 179 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2296 2296
Size: 3929
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 468 578 790 463
Size: 5 110 212 3602
Fragments arranged by size:
3602 212 110 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 1984 2476 2774 1105
Size: 879 492 298 2260
Fragments arranged by size:
2260 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3633 3633
Size: 3929
BsaAI yAC'GTr
Cuts at: 141 381 1282 141
Size: 240 901 2788
Fragments arranged by size:
2788 901 240
BsaBI GATnn'nnATC
Cuts at: 6 1443 3480 6
Size: 1437 2037 455
Fragments arranged by size:
2037 1437 455
BsaHI Gr'CG_yC
Cuts at: 161 167 299 329 394 412 626 1360 1608
Size: 6 132 30 65 18 214 734 248
Cuts at: 1608 2310 3806 161
Size: 702 1496 284
Fragments arranged by size:
1496 734 702 284 248 214 132 65
30 18 6
BsaWI w'CCGG_w
Cuts at: 1118 1639 2317 2591 2738 3149 1118
Size: 521 678 274 147 411 1898
Fragments arranged by size:
1898 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 418 448 763 793 418
Size: 30 315 30 3554
Fragments arranged by size:
3554 315 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 581 581
Size: 3929
BseYI C'CCAG_C
Cuts at: 156 616 693 1354 2112 2689 3185 156
Size: 460 77 661 758 577 496 900
Fragments arranged by size:
900 758 661 577 496 460 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 288 288
Size: 3929
BsiEI CG_ry'CG
Cuts at: 104 1471 1517 2725 104
Size: 1367 46 1208 1308
Fragments arranged by size:
1367 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 251 320 386 943 1721 1911 2703 251
Size: 69 66 557 778 190 792 1477
Fragments arranged by size:
1477 792 778 557 190 69 66
Bsp1286I G_dGCh'C
Cuts at: 126 251 320 386 449 742 943 1554 1647
Size: 125 69 66 63 293 201 611 93
Cuts at: 1647 1721 1911 1973 2703 126
Size: 74 190 62 730 1352
Fragments arranged by size:
1352 730 611 293 201 190 125 93
74 69 66 63 62
BspEI T'CCGG_A
Cuts at: 3149 3149
Size: 3929
BspHI T'CATG_A
Cuts at: 3105 3105
Size: 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 413 803 843 979 1193 1495 1876 413
Size: 390 40 136 214 302 381 2466
Fragments arranged by size:
2466 390 381 302 214 136 40
BsrBI CCG'CTC
Cuts at: 50 659 2221 2275 3759 3924 50
Size: 609 1562 54 1484 165 55
Fragments arranged by size:
1562 1484 609 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1841 1841
Size: 3929
BsrFI r'CCGG_y
Cuts at: 488 1927 2108 2317 488
Size: 1439 181 209 2100
Fragments arranged by size:
2100 1439 209 181
BssHII G'CGCG_C
Cuts at: 715 715
Size: 3929
BssSI C'ACGA_G
Cuts at: 303 2200 2558 303
Size: 1897 358 1674
Fragments arranged by size:
1897 1674 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1306 1306
Size: 3929
BstBI TT'CG_AA
Cuts at: 2290 2290
Size: 3929
BstYI r'GATC_y
Cuts at: 1 860 954 1421 1444 1779 2025 3037 3123
Size: 859 94 467 23 335 246 1012 86
Cuts at: 3123 3135 3481 1
Size: 12 346 449
Fragments arranged by size:
1012 859 467 449 346 335 246 94
86 23 12
BtgI C'CryG_G
Cuts at: 107 170 398 107
Size: 63 228 3638
Fragments arranged by size:
3638 228 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1879 2060 2223 1879
Size: 181 163 3585
Fragments arranged by size:
3585 181 163
BtsI GCAGTG_nn'
Cuts at: 1172 1172
Size: 3929
ClaI AT'CG_AT
Cuts at: 680 680
Size: 3929
DraI TTT'AAA
Cuts at: 928 928
Size: 3929
DrdI GACnn_nn'nnGTC
Cuts at: 1635 2493 1635
Size: 858 3071
EaeI y'GGCC_r
Cuts at: 110 189 242 521 1514 1688 2079 2106 3451
Size: 79 53 279 993 174 391 27 1345
Cuts at: 3451 110
Size: 588
Fragments arranged by size:
1345 993 588 391 279 174 79 53
27
EagI C'GGCC_G
Cuts at: 1514 1514
Size: 3929
EarI CTCTTCn'nnn_
Cuts at: 1952 2162 1952
Size: 210 3719
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 677 2137 2447 2593 3739 677
Size: 1460 310 146 1146 867
Fragments arranged by size:
1460 1146 867 310 146
EcoICRI GAG'CTC
Cuts at: 941 941
Size: 3929
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 463 468 578 790 1754 2186 2933 3201
Size: 220 5 110 212 964 432 747 268
Cuts at: 3201 243
Size: 971
Fragments arranged by size:
971 964 747 432 268 220 212 110
5
EcoO109I rG'GnC_Cy
Cuts at: 122 123 206 1068 122
Size: 1 83 862 2983
Fragments arranged by size:
2983 862 83 1
EcoRI G'AATT_C
Cuts at: 933 933
Size: 3929
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1948 1948
Size: 3929
FspI TGC'GCA
Cuts at: 1710 1710
Size: 3929
HaeII r_GCGC'y
Cuts at: 164 302 332 397 415 629 1363 1611 2633
Size: 138 30 65 18 214 734 248 1022
Cuts at: 2633 3612 164
Size: 979 481
Fragments arranged by size:
1022 979 734 481 248 214 138 65
30 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1771 1801 1803 1833 1771
Size: 30 2 30 3867
Fragments arranged by size:
3867 30 30 2
HincII GTy'rAC
Cuts at: 968 968
Size: 3929
KasI G'GCGC_C
Cuts at: 160 298 328 393 411 625 1359 1607 160
Size: 138 30 65 18 214 734 248 2482
Fragments arranged by size:
2482 734 248 214 138 65 30 18
KpnI G_GTAC'C
Cuts at: 949 949
Size: 3929
MluI A'CGCG_T
Cuts at: 3362 3362
Size: 3929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2600 2784 2600
Size: 184 3745
MscI TGG'CCA
Cuts at: 112 1690 112
Size: 1578 2351
MslI CAynn'nnrTG
Cuts at: 1918 2045 1918
Size: 127 3802
MspA1I CmG'CkG
Cuts at: 172 346 516 1040 1354 1714 2727 2972 172
Size: 174 170 524 314 360 1013 245 1129
Fragments arranged by size:
1129 1013 524 360 314 245 174 170
NaeI GCC'GGC
Cuts at: 490 2110 490
Size: 1620 2309
NarI GG'CG_CC
Cuts at: 161 299 329 394 412 626 1360 1608 161
Size: 138 30 65 18 214 734 248 2482
Fragments arranged by size:
2482 734 248 214 138 65 30 18
NcoI C'CATG_G
Cuts at: 107 107
Size: 3929
NgoMIV G'CCGG_C
Cuts at: 488 2108 488
Size: 1620 2309
NspI r_CATG'y
Cuts at: 432 795 982 1482 2389 432
Size: 363 187 500 907 1972
Fragments arranged by size:
1972 907 500 363 187
PasI CC'CwG_GG
Cuts at: 126 210 126
Size: 84 3845
PciI A'CATG_T
Cuts at: 791 2385 791
Size: 1594 2335
PflMI CCAn_nnn'nTGG
Cuts at: 762 762
Size: 3929
PmeI GTTT'AAAC
Cuts at: 928 928
Size: 3929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 769 801 3068 3100 769
Size: 32 2267 32 1598
Fragments arranged by size:
2267 1598 32 32
PpuMI rG'GwC_Cy
Cuts at: 206 206
Size: 3929
PspOMI G'GGCC_C
Cuts at: 122 122
Size: 3929
PstI C_TGCA'G
Cuts at: 976 1661 976
Size: 685 3244
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3929
PvuII CAG'CTG
Cuts at: 1354 1714 1354
Size: 360 3569
RsrII CG'GwC_CG
Cuts at: 2124 2124
Size: 3929
SacI G_AGCT'C
Cuts at: 943 943
Size: 3929
SacII CC_GC'GG
Cuts at: 173 173
Size: 3929
SalI G'TCGA_C
Cuts at: 966 966
Size: 3929
SanDI GG'GwC_CC
Cuts at: 206 206
Size: 3929
SapI GCTCTTCn'nnn_
Cuts at: 1952 2162 1952
Size: 210 3719
SbfI CC_TGCA'GG
Cuts at: 976 976
Size: 3929
ScaI AGT'ACT
Cuts at: 3446 3446
Size: 3929
SfcI C'TryA_G
Cuts at: 33 972 1657 2650 2841 33
Size: 939 685 993 191 1121
Fragments arranged by size:
1121 993 939 685 191
SfoI GGC'GCC
Cuts at: 162 300 330 395 413 627 1361 1609 162
Size: 138 30 65 18 214 734 248 2482
Fragments arranged by size:
2482 734 248 214 138 65 30 18
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
SmaI CCC'GGG
Cuts at: 951 951
Size: 3929
SmlI C'TyrA_G
Cuts at: 1084 1999 2491 2753 1084
Size: 915 492 262 2260
Fragments arranged by size:
2260 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3929
SphI G_CATG'C
Cuts at: 982 1482 982
Size: 500 3429
StyI C'CwwG_G
Cuts at: 107 281 1063 1417 107
Size: 174 782 354 2619
Fragments arranged by size:
2619 782 354 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 923 1512 1645 2104 2313 923
Size: 589 133 459 209 2539
Fragments arranged by size:
2539 589 459 209 133
TatI w'GTAC_w
Cuts at: 3444 3444
Size: 3929
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 547 579 3446 3478 547
Size: 32 2867 32 998
Fragments arranged by size:
2867 998 32 32
Tth111I GACn'n_nGTC
Cuts at: 1726 1726
Size: 3929
XbaI T'CTAG_A
Cuts at: 960 960
Size: 3929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 123 701 1424 123
Size: 578 723 2628
Fragments arranged by size:
2628 723 578
XmaI C'CCGG_G
Cuts at: 949 949
Size: 3929
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
Bsu36I CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PshAI PsiI PspXI PsrI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI