Restriction map for
pF1KB8335
(Circular) (Six-base) MAPSORT of: pF1KB8335.seq Check: 9523 from: 1 to: 4433
pF1KB8335 4433 bp
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
AccI GT'mk_AC
Cuts at: 521 1471 521
Size: 950 3483
Acc65I G'GTAC_C
Cuts at: 1449 1449
Size: 4433
AclI AA'CG_TT
Cuts at: 4273 4273
Size: 4433
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 682 2258 2690 3437 3705 164
Size: 518 1576 432 747 268 892
Fragments arranged by size:
1576 892 747 518 432 268
AfeI AGC'GCT
Cuts at: 1318 1318
Size: 4433
AflIII A'CryG_T
Cuts at: 1785 2889 3866 1785
Size: 1104 977 2352
Fragments arranged by size:
2352 1104 977
AgeI A'CCGG_T
Cuts at: 2821 2821
Size: 4433
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 628 1178 628
Size: 550 3883
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 451 483 451
Size: 32 4401
AlwNI CAG_nnn'CTG
Cuts at: 288 621 1209 1738 3305 288
Size: 333 588 529 1567 1416
Fragments arranged by size:
1567 1416 588 529 333
ApaLI G'TGCA_C
Cuts at: 427 3203 427
Size: 2776 1657
ApoI r'AATT_y
Cuts at: 646 1437 3786 4042 646
Size: 791 2349 256 1037
Fragments arranged by size:
2349 1037 791 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4433
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4433
AvaI C'yCGr_G
Cuts at: 133 1453 133
Size: 1320 3113
BamHI G'GATC_C
Cuts at: 592 1458 592
Size: 866 3567
BanI G'GyrC_C
Cuts at: 1449 1863 2111 2146 1449
Size: 414 248 35 3736
Fragments arranged by size:
3736 414 248 35
BanII G_rGCy'C
Cuts at: 193 278 1447 2477 193
Size: 85 1169 1030 2149
Fragments arranged by size:
2149 1169 1030 85
BbeI G_GCGC'C
Cuts at: 1867 2115 1867
Size: 248 4185
BbsI GAAGACnn'nnnn_
Cuts at: 506 506
Size: 4433
BciVI GTATCCnnnnn_n'
Cuts at: 2331 3092 4433 2331
Size: 761 1341 2331
Fragments arranged by size:
2331 1341 761
BclI T'GATC_A
Cuts at: 1953 1953
Size: 4433
BglII A'GATC_T
Cuts at: 1948 3985 1948
Size: 2037 2396
BlpI GC'TnA_GC
Cuts at: 736 871 919 1545 736
Size: 135 48 626 3624
Fragments arranged by size:
3624 626 135 48
Bme1580I G_kGCm'C
Cuts at: 431 2058 2151 3207 431
Size: 1627 93 1056 1657
Fragments arranged by size:
1657 1627 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1383 1645 1726 2061 4205 1383
Size: 262 81 335 2144 1611
Fragments arranged by size:
2144 1611 335 262 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 207 2800 207
Size: 2593 1840
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 796 810 1150 1162 1256 244
Size: 552 14 340 12 94 3421
Fragments arranged by size:
3421 552 340 94 14 12
Bpu10I CC'TnA_GC
Cuts at: 932 932
Size: 4433
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 744 961 1609 2488 2980 3278 744
Size: 217 648 879 492 298 1899
Fragments arranged by size:
1899 879 648 492 298 217
BsaI GGTCTCn'nnnn_
Cuts at: 107 1231 4137 107
Size: 1124 2906 403
Fragments arranged by size:
2906 1124 403
BsaAI yAC'GTr
Cuts at: 1786 1786
Size: 4433
BsaBI GATnn'nnATC
Cuts at: 6 1947 3984 6
Size: 1941 2037 455
Fragments arranged by size:
2037 1941 455
BsaHI Gr'CG_yC
Cuts at: 1864 2112 2814 4310 1864
Size: 248 702 1496 1987
Fragments arranged by size:
1987 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1622 2143 2821 3095 3242 3653 1622
Size: 521 678 274 147 411 2402
Fragments arranged by size:
2402 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 424 451 454 481 1062 1092 424
Size: 27 3 27 581 30 3765
Fragments arranged by size:
3765 581 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 340 340
Size: 4433
BseYI C'CCAG_C
Cuts at: 1073 1858 2616 3193 3689 1073
Size: 785 758 577 496 1817
Fragments arranged by size:
1817 785 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1322 1322
Size: 4433
BsiEI CG_ry'CG
Cuts at: 104 1975 2021 3229 104
Size: 1871 46 1208 1308
Fragments arranged by size:
1871 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 431 1447 2225 2415 3207 431
Size: 1016 778 190 792 1657
Fragments arranged by size:
1657 1016 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 193 278 431 1447 2058 2151 2225 2415 2477
Size: 85 153 1016 611 93 74 190 62
Cuts at: 2477 3207 193
Size: 730 1419
Fragments arranged by size:
1419 1016 730 611 190 153 93 85
74 62
BspEI T'CCGG_A
Cuts at: 3653 3653
Size: 4433
BspHI T'CATG_A
Cuts at: 3609 3609
Size: 4433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 758 920 1217 1483 1697 1999 2380 758
Size: 162 297 266 214 302 381 2811
Fragments arranged by size:
2811 381 302 297 266 214 162
BsrBI CCG'CTC
Cuts at: 50 1125 1278 2725 2779 4263 4428 50
Size: 1075 153 1447 54 1484 165 55
Fragments arranged by size:
1484 1447 1075 165 153 55 54
BsrDI GCAATG_nn'
Cuts at: 2345 2345
Size: 4433
BsrFI r'CCGG_y
Cuts at: 794 2431 2612 2821 794
Size: 1637 181 209 2406
Fragments arranged by size:
2406 1637 209 181
BsrGI T'GTAC_A
Cuts at: 905 905
Size: 4433
BssHII G'CGCG_C
Cuts at: 937 937
Size: 4433
BssSI C'ACGA_G
Cuts at: 433 2704 3062 433
Size: 2271 358 1804
Fragments arranged by size:
2271 1804 358
BstAPI GCAn_nnn'nTGC
Cuts at: 621 1209 1358 1810 621
Size: 588 149 452 3244
Fragments arranged by size:
3244 588 452 149
BstBI TT'CG_AA
Cuts at: 2794 2794
Size: 4433
BstEII G'GTnAC_C
Cuts at: 294 294
Size: 4433
BstXI CCAn_nnnn'nTGG
Cuts at: 420 1118 420
Size: 698 3735
BstYI r'GATC_y
Cuts at: 1 592 1106 1458 1925 1948 2283 2529 3541
Size: 591 514 352 467 23 335 246 1012
Cuts at: 3541 3627 3639 3985 1
Size: 86 12 346 449
Fragments arranged by size:
1012 591 514 467 449 352 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 581 926 581
Size: 345 4088
BtgI C'CryG_G
Cuts at: 107 300 107
Size: 193 4240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2383 2564 2727 2383
Size: 181 163 4089
Fragments arranged by size:
4089 181 163
BtsI GCAGTG_nn'
Cuts at: 344 1003 1271 1305 1676 344
Size: 659 268 34 371 3101
Fragments arranged by size:
3101 659 371 268 34
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 302 337 302
Size: 35 4398
DraI TTT'AAA
Cuts at: 1432 1432
Size: 4433
DrdI GACnn_nn'nnGTC
Cuts at: 2139 2997 2139
Size: 858 3575
EaeI y'GGCC_r
Cuts at: 2018 2192 2583 2610 3955 2018
Size: 174 391 27 1345 2496
Fragments arranged by size:
2496 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2018 2018
Size: 4433
EarI CTCTTCn'nnn_
Cuts at: 2456 2666 2456
Size: 210 4223
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2641 2951 3097 4243 2641
Size: 310 146 1146 2831
Fragments arranged by size:
2831 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1445 1445
Size: 4433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 244 682 796 810 1150 1162 1256 2258
Size: 80 438 114 14 340 12 94 1002
Cuts at: 2258 2690 3437 3705 164
Size: 432 747 268 892
Fragments arranged by size:
1002 892 747 438 432 340 268 114
94 80 14 12
EcoO109I rG'GnC_Cy
Cuts at: 309 666 1089 1206 1572 309
Size: 357 423 117 366 3170
Fragments arranged by size:
3170 423 366 357 117
EcoRI G'AATT_C
Cuts at: 1437 1437
Size: 4433
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 4433
FspI TGC'GCA
Cuts at: 166 2214 166
Size: 2048 2385
HaeII r_GCGC'y
Cuts at: 1320 1867 2115 3137 4116 1320
Size: 547 248 1022 979 1637
Fragments arranged by size:
1637 1022 979 547 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 694 726 959 991 2275 2305 2307 2337 694
Size: 32 233 32 1284 30 2 30 2790
Fragments arranged by size:
2790 1284 233 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1472 1472
Size: 4433
HindIII A'AGCT_T
Cuts at: 979 979
Size: 4433
KasI G'GCGC_C
Cuts at: 1863 2111 1863
Size: 248 4185
KpnI G_GTAC'C
Cuts at: 1453 1453
Size: 4433
MfeI C'AATT_G
Cuts at: 1160 1160
Size: 4433
MluI A'CGCG_T
Cuts at: 3866 3866
Size: 4433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 860 1079 3104 3288 860
Size: 219 2025 184 2005
Fragments arranged by size:
2025 2005 219 184
MscI TGG'CCA
Cuts at: 2194 2194
Size: 4433
MslI CAynn'nnrTG
Cuts at: 418 424 2422 2549 418
Size: 6 1998 127 2302
Fragments arranged by size:
2302 1998 127 6
MspA1I CmG'CkG
Cuts at: 210 1017 1286 1544 1858 2218 3231 3476 210
Size: 807 269 258 314 360 1013 245 1167
Fragments arranged by size:
1167 1013 807 360 314 269 258 245
NaeI GCC'GGC
Cuts at: 796 2614 796
Size: 1818 2615
NarI GG'CG_CC
Cuts at: 1864 2112 1864
Size: 248 4185
NcoI C'CATG_G
Cuts at: 107 300 107
Size: 193 4240
NgoMIV G'CCGG_C
Cuts at: 794 2612 794
Size: 1818 2615
NspI r_CATG'y
Cuts at: 240 1186 1486 1986 2893 240
Size: 946 300 500 907 1780
Fragments arranged by size:
1780 946 907 500 300
PasI CC'CwG_GG
Cuts at: 1410 1410
Size: 4433
PciI A'CATG_T
Cuts at: 2889 2889
Size: 4433
PmeI GTTT'AAAC
Cuts at: 1432 1432
Size: 4433
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 451 483 3572 3604 451
Size: 32 3089 32 1280
Fragments arranged by size:
3089 1280 32 32
PpuMI rG'GwC_Cy
Cuts at: 309 1089 1206 309
Size: 780 117 3536
Fragments arranged by size:
3536 780 117
PshAI GACnn'nnGTC
Cuts at: 1396 1396
Size: 4433
PstI C_TGCA'G
Cuts at: 379 626 818 1220 1300 1354 1480 2165 379
Size: 247 192 402 80 54 126 685 2647
Fragments arranged by size:
2647 685 402 247 192 126 80 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4433
PvuII CAG'CTG
Cuts at: 1017 1858 2218 1017
Size: 841 360 3232
Fragments arranged by size:
3232 841 360
RsrII CG'GwC_CG
Cuts at: 2628 2628
Size: 4433
SacI G_AGCT'C
Cuts at: 1447 1447
Size: 4433
SalI G'TCGA_C
Cuts at: 1470 1470
Size: 4433
SapI GCTCTTCn'nnn_
Cuts at: 2456 2666 2456
Size: 210 4223
SbfI CC_TGCA'GG
Cuts at: 1480 1480
Size: 4433
ScaI AGT'ACT
Cuts at: 677 3950 677
Size: 3273 1160
SexAI A'CCwGG_T
Cuts at: 1091 1091
Size: 4433
SfcI C'TryA_G
Cuts at: 33 375 622 814 1216 1242 1296 1350 1476
Size: 342 247 192 402 26 54 54 126
Cuts at: 1476 2161 3154 3345 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 402 342 247 192 191
126 54 54 26
SfoI GGC'GCC
Cuts at: 1865 2113 1865
Size: 248 4185
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4433
SmaI CCC'GGG
Cuts at: 135 1455 135
Size: 1320 3113
SmlI C'TyrA_G
Cuts at: 723 976 1588 2503 2995 3257 723
Size: 253 612 915 492 262 1899
Fragments arranged by size:
1899 915 612 492 262 253
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4433
SphI G_CATG'C
Cuts at: 1486 1986 1486
Size: 500 3933
StuI AGG'CCT
Cuts at: 439 439
Size: 4433
StyI C'CwwG_G
Cuts at: 107 300 962 1567 1921 107
Size: 193 662 605 354 2619
Fragments arranged by size:
2619 662 605 354 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 313 2016 2149 2608 2817 313
Size: 1703 133 459 209 1929
Fragments arranged by size:
1929 1703 459 209 133
TatI w'GTAC_w
Cuts at: 675 905 3948 675
Size: 230 3043 1160
Fragments arranged by size:
3043 1160 230
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 424 456 3950 3982 424
Size: 32 3494 32 875
Fragments arranged by size:
3494 875 32 32
Tth111I GACn'n_nGTC
Cuts at: 505 2230 505
Size: 1725 2708
XbaI T'CTAG_A
Cuts at: 1464 1464
Size: 4433
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 420 565 1928 114
Size: 306 145 1363 2619
Fragments arranged by size:
2619 1363 306 145
XmaI C'CCGG_G
Cuts at: 133 1453 133
Size: 1320 3113
XmnI GAAnn'nnTTC
Cuts at: 361 406 361
Size: 45 4388
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PmeI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmI BsmBI BstZ17I ClaI DraIII EcoNI EcoRV FseI
FspAI HpaI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI