Restriction map for
pF1KB8334
(Circular) (Six-base) MAPSORT of: pF1KB8334.seq Check: 7116 from: 1 to: 3677
pF1KB8334 3677 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 715 715
Size: 3677
Acc65I G'GTAC_C
Cuts at: 693 693
Size: 3677
AclI AA'CG_TT
Cuts at: 3517 3517
Size: 3677
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1502 1934 2681 2949 1502
Size: 432 747 268 2230
Fragments arranged by size:
2230 747 432 268
AflIII A'CryG_T
Cuts at: 1029 2133 3110 1029
Size: 1104 977 1596
Fragments arranged by size:
1596 1104 977
AgeI A'CCGG_T
Cuts at: 2065 2065
Size: 3677
AlwNI CAG_nnn'CTG
Cuts at: 982 2549 982
Size: 1567 2110
ApaLI G'TGCA_C
Cuts at: 2447 2447
Size: 3677
ApoI r'AATT_y
Cuts at: 681 3030 3286 681
Size: 2349 256 1072
Fragments arranged by size:
2349 1072 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3677
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3677
AvaI C'yCGr_G
Cuts at: 531 697 531
Size: 166 3511
BamHI G'GATC_C
Cuts at: 702 702
Size: 3677
BanI G'GyrC_C
Cuts at: 693 1107 1355 1390 693
Size: 414 248 35 2980
Fragments arranged by size:
2980 414 248 35
BanII G_rGCy'C
Cuts at: 691 1721 691
Size: 1030 2647
BbeI G_GCGC'C
Cuts at: 1111 1359 1111
Size: 248 3429
BbsI GAAGACnn'nnnn_
Cuts at: 344 344
Size: 3677
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 109 143 172 206 423 457 109
Size: 34 29 34 217 34 3329
Fragments arranged by size:
3329 217 34 34 34 29
BciVI GTATCCnnnnn_n'
Cuts at: 1575 2336 3677 1575
Size: 761 1341 1575
Fragments arranged by size:
1575 1341 761
BclI T'GATC_A
Cuts at: 1197 1197
Size: 3677
BglII A'GATC_T
Cuts at: 568 1192 3229 568
Size: 624 2037 1016
Fragments arranged by size:
2037 1016 624
BlpI GC'TnA_GC
Cuts at: 789 789
Size: 3677
Bme1580I G_kGCm'C
Cuts at: 529 1302 1395 2451 529
Size: 773 93 1056 1755
Fragments arranged by size:
1755 1056 773 93
BmrI ACTGGGnnnn_n'
Cuts at: 889 970 1305 3449 889
Size: 81 335 2144 1117
Fragments arranged by size:
2144 1117 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2044 2044
Size: 3677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 403
Size: 3677
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 1732 2224 2522 853
Size: 879 492 298 2008
Fragments arranged by size:
2008 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 129 3381 129
Size: 3252 425
BsaAI yAC'GTr
Cuts at: 1030 1030
Size: 3677
BsaBI GATnn'nnATC
Cuts at: 6 1191 3228 6
Size: 1185 2037 455
Fragments arranged by size:
2037 1185 455
BsaHI Gr'CG_yC
Cuts at: 122 1108 1356 2058 3554 122
Size: 986 248 702 1496 245
Fragments arranged by size:
1496 986 702 248 245
BsaWI w'CCGG_w
Cuts at: 866 1387 2065 2339 2486 2897 866
Size: 521 678 274 147 411 1646
Fragments arranged by size:
1646 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1102 1860 2437 2933 1102
Size: 758 577 496 1846
Fragments arranged by size:
1846 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1219 1265 2473 104
Size: 1115 46 1208 1308
Fragments arranged by size:
1308 1208 1115 46
BsiHKAI G_wGCw'C
Cuts at: 153 691 1469 1659 2451 153
Size: 538 778 190 792 1379
Fragments arranged by size:
1379 792 778 538 190
BsmBI CGTCTCn'nnnn_
Cuts at: 110 110
Size: 3677
Bsp1286I G_dGCh'C
Cuts at: 153 529 691 1302 1395 1469 1659 1721 2451
Size: 376 162 611 93 74 190 62 730
Cuts at: 2451 153
Size: 1379
Fragments arranged by size:
1379 730 611 376 190 162 93 74
62
BspEI T'CCGG_A
Cuts at: 2897 2897
Size: 3677
BspHI T'CATG_A
Cuts at: 2853 2853
Size: 3677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 727 941 1243 1624 727
Size: 214 302 381 2780
Fragments arranged by size:
2780 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1969 2023 3507 3672 50
Size: 1919 54 1484 165 55
Fragments arranged by size:
1919 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1589 1589
Size: 3677
BsrFI r'CCGG_y
Cuts at: 1675 1856 2065 1675
Size: 181 209 3287
Fragments arranged by size:
3287 209 181
BssSI C'ACGA_G
Cuts at: 562 1948 2306 562
Size: 1386 358 1933
Fragments arranged by size:
1933 1386 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1054 1054
Size: 3677
BstBI TT'CG_AA
Cuts at: 2038 2038
Size: 3677
BstYI r'GATC_y
Cuts at: 1 167 568 702 1169 1192 1527 1773 2785
Size: 166 401 134 467 23 335 246 1012
Cuts at: 2785 2871 2883 3229 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 401 346 335 246 166
134 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 656 656
Size: 3677
BtgI C'CryG_G
Cuts at: 140 375 140
Size: 235 3442
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1627 1808 1971 1627
Size: 181 163 3333
Fragments arranged by size:
3333 181 163
BtsI GCAGTG_nn'
Cuts at: 920 920
Size: 3677
DraI TTT'AAA
Cuts at: 676 676
Size: 3677
DrdI GACnn_nn'nnGTC
Cuts at: 1383 2241 1383
Size: 858 2819
EaeI y'GGCC_r
Cuts at: 1262 1436 1827 1854 3199 1262
Size: 174 391 27 1345 1740
Fragments arranged by size:
1740 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1262 1262
Size: 3677
EarI CTCTTCn'nnn_
Cuts at: 206 256 635 1700 1910 206
Size: 50 379 1065 210 1973
Fragments arranged by size:
1973 1065 379 210 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1885 2195 2341 3487 1885
Size: 310 146 1146 2075
Fragments arranged by size:
2075 1146 310 146
EcoICRI GAG'CTC
Cuts at: 689 689
Size: 3677
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 1502 1934 2681 2949 403
Size: 1099 432 747 268 1131
Fragments arranged by size:
1131 1099 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 129 129
Size: 3677
EcoO109I rG'GnC_Cy
Cuts at: 816 816
Size: 3677
EcoRI G'AATT_C
Cuts at: 681 681
Size: 3677
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1696 1696
Size: 3677
FspI TGC'GCA
Cuts at: 1458 1458
Size: 3677
HaeII r_GCGC'y
Cuts at: 1111 1359 2381 3360 1111
Size: 248 1022 979 1428
Fragments arranged by size:
1428 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1519 1549 1551 1581 1519
Size: 30 2 30 3615
Fragments arranged by size:
3615 30 30 2
HincII GTy'rAC
Cuts at: 716 716
Size: 3677
KasI G'GCGC_C
Cuts at: 1107 1355 1107
Size: 248 3429
KpnI G_GTAC'C
Cuts at: 697 697
Size: 3677
MluI A'CGCG_T
Cuts at: 3110 3110
Size: 3677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2348 2532 2348
Size: 184 3493
MscI TGG'CCA
Cuts at: 1438 1438
Size: 3677
MslI CAynn'nnrTG
Cuts at: 1666 1793 1666
Size: 127 3550
MspA1I CmG'CkG
Cuts at: 627 788 1102 1462 2475 2720 627
Size: 161 314 360 1013 245 1584
Fragments arranged by size:
1584 1013 360 314 245 161
NaeI GCC'GGC
Cuts at: 1858 1858
Size: 3677
NarI GG'CG_CC
Cuts at: 1108 1356 1108
Size: 248 3429
NgoMIV G'CCGG_C
Cuts at: 1856 1856
Size: 3677
NspI r_CATG'y
Cuts at: 595 730 1230 2137 595
Size: 135 500 907 2135
Fragments arranged by size:
2135 907 500 135
PciI A'CATG_T
Cuts at: 2133 2133
Size: 3677
PmeI GTTT'AAAC
Cuts at: 676 676
Size: 3677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2816 2848 2816
Size: 32 3645
PstI C_TGCA'G
Cuts at: 724 1409 724
Size: 685 2992
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3677
PvuII CAG'CTG
Cuts at: 627 1102 1462 627
Size: 475 360 2842
Fragments arranged by size:
2842 475 360
RsrII CG'GwC_CG
Cuts at: 1872 1872
Size: 3677
SacI G_AGCT'C
Cuts at: 691 691
Size: 3677
SalI G'TCGA_C
Cuts at: 714 714
Size: 3677
SapI GCTCTTCn'nnn_
Cuts at: 1700 1910 1700
Size: 210 3467
SbfI CC_TGCA'GG
Cuts at: 724 724
Size: 3677
ScaI AGT'ACT
Cuts at: 3194 3194
Size: 3677
SfcI C'TryA_G
Cuts at: 33 720 1405 2398 2589 33
Size: 687 685 993 191 1121
Fragments arranged by size:
1121 993 687 685 191
SfoI GGC'GCC
Cuts at: 1109 1357 1109
Size: 248 3429
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3677
SmaI CCC'GGG
Cuts at: 533 699 533
Size: 166 3511
SmlI C'TyrA_G
Cuts at: 832 1747 2239 2501 832
Size: 915 492 262 2008
Fragments arranged by size:
2008 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3677
SphI G_CATG'C
Cuts at: 730 1230 730
Size: 500 3177
StuI AGG'CCT
Cuts at: 178 178
Size: 3677
StyI C'CwwG_G
Cuts at: 811 1165 811
Size: 354 3323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1260 1393 1852 2061 1260
Size: 133 459 209 2876
Fragments arranged by size:
2876 459 209 133
TatI w'GTAC_w
Cuts at: 3192 3192
Size: 3677
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3194 3226 3194
Size: 32 3645
Tth111I GACn'n_nGTC
Cuts at: 1474 1474
Size: 3677
XbaI T'CTAG_A
Cuts at: 708 708
Size: 3677
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1172 1172
Size: 3677
XmaI C'CCGG_G
Cuts at: 531 697 531
Size: 166 3511
XmnI GAAnn'nnTTC
Cuts at: 244 471 244
Size: 227 3450
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI