Restriction map for
pF1KB8333
(Circular) (Six-base) MAPSORT of: pF1KB8333.seq Check: 4939 from: 1 to: 4052
pF1KB8333 4052 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1090 1090
Size: 4052
Acc65I G'GTAC_C
Cuts at: 522 1068 522
Size: 546 3506
AclI AA'CG_TT
Cuts at: 3892 3892
Size: 4052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 1877 2309 3056 3324 730
Size: 1147 432 747 268 1458
Fragments arranged by size:
1458 1147 747 432 268
AflIII A'CryG_T
Cuts at: 957 959 1404 2508 3485 957
Size: 2 445 1104 977 1524
Fragments arranged by size:
1524 1104 977 445 2
AgeI A'CCGG_T
Cuts at: 2440 2440
Size: 4052
AhdI GACnn_n'nnGTC
Cuts at: 933 933
Size: 4052
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 196 196
Size: 4052
AlwNI CAG_nnn'CTG
Cuts at: 1357 2924 1357
Size: 1567 2485
ApaLI G'TGCA_C
Cuts at: 2822 2822
Size: 4052
ApoI r'AATT_y
Cuts at: 1056 3405 3661 1056
Size: 2349 256 1447
Fragments arranged by size:
2349 1447 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4052
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
AvaI C'yCGr_G
Cuts at: 268 1072 268
Size: 804 3248
BamHI G'GATC_C
Cuts at: 744 1077 744
Size: 333 3719
BanI G'GyrC_C
Cuts at: 148 199 522 653 1068 1482 1730 1765 148
Size: 51 323 131 415 414 248 35 2435
Fragments arranged by size:
2435 415 414 323 248 131 51 35
BanII G_rGCy'C
Cuts at: 542 946 1066 2096 542
Size: 404 120 1030 2498
Fragments arranged by size:
2498 1030 404 120
BbeI G_GCGC'C
Cuts at: 203 657 1486 1734 203
Size: 454 829 248 2521
Fragments arranged by size:
2521 829 454 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 325 359 325
Size: 34 4018
BciVI GTATCCnnnnn_n'
Cuts at: 1950 2711 4052 1950
Size: 761 1341 1950
Fragments arranged by size:
1950 1341 761
BclI T'GATC_A
Cuts at: 1572 1572
Size: 4052
BglII A'GATC_T
Cuts at: 1567 3604 1567
Size: 2037 2015
BlpI GC'TnA_GC
Cuts at: 1164 1164
Size: 4052
Bme1580I G_kGCm'C
Cuts at: 513 642 1013 1677 1770 2826 513
Size: 129 371 664 93 1056 1739
Fragments arranged by size:
1739 1056 664 371 129 93
BmrI ACTGGGnnnn_n'
Cuts at: 1264 1345 1680 3824 1264
Size: 81 335 2144 1492
Fragments arranged by size:
2144 1492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 4052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 138
Size: 4052
Bpu10I CC'TnA_GC
Cuts at: 251 844 251
Size: 593 3459
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1228 2107 2599 2897 1228
Size: 879 492 298 2383
Fragments arranged by size:
2383 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3756 3756
Size: 4052
BsaAI yAC'GTr
Cuts at: 960 1405 960
Size: 445 3607
BsaBI GATnn'nnATC
Cuts at: 6 1566 3603 6
Size: 1560 2037 455
Fragments arranged by size:
2037 1560 455
BsaHI Gr'CG_yC
Cuts at: 200 360 654 1483 1731 2433 3929 200
Size: 160 294 829 248 702 1496 323
Fragments arranged by size:
1496 829 702 323 294 248 160
BsaWI w'CCGG_w
Cuts at: 1241 1762 2440 2714 2861 3272 1241
Size: 521 678 274 147 411 2021
Fragments arranged by size:
2021 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 979 1009 979
Size: 30 4022
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 546 745 546
Size: 199 3853
BseYI C'CCAG_C
Cuts at: 1477 2235 2812 3308 1477
Size: 758 577 496 2221
Fragments arranged by size:
2221 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 715
Size: 4052
BsiEI CG_ry'CG
Cuts at: 104 915 1594 1640 2848 104
Size: 811 679 46 1208 1308
Fragments arranged by size:
1308 1208 811 679 46
BsiHKAI G_wGCw'C
Cuts at: 946 1066 1844 2034 2826 946
Size: 120 778 190 792 2172
Fragments arranged by size:
2172 792 778 190 120
BsmI GAATG_Cn'
Cuts at: 427 427
Size: 4052
Bsp1286I G_dGCh'C
Cuts at: 513 542 642 946 1013 1066 1677 1770 1844
Size: 29 100 304 67 53 611 93 74
Cuts at: 1844 2034 2096 2826 513
Size: 190 62 730 1739
Fragments arranged by size:
1739 730 611 304 190 100 93 74
67 62 53 29
BspEI T'CCGG_A
Cuts at: 3272 3272
Size: 4052
BspHI T'CATG_A
Cuts at: 3228 3228
Size: 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 998 1102 1316 1618 1999 998
Size: 104 214 302 381 3051
Fragments arranged by size:
3051 381 302 214 104
BsrBI CCG'CTC
Cuts at: 50 2344 2398 3882 4047 50
Size: 2294 54 1484 165 55
Fragments arranged by size:
2294 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 173 1964 173
Size: 1791 2261
BsrFI r'CCGG_y
Cuts at: 2050 2231 2440 2050
Size: 181 209 3662
Fragments arranged by size:
3662 209 181
BssSI C'ACGA_G
Cuts at: 643 2323 2681 643
Size: 1680 358 2014
Fragments arranged by size:
2014 1680 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1429 1429
Size: 4052
BstBI TT'CG_AA
Cuts at: 2413 2413
Size: 4052
BstYI r'GATC_y
Cuts at: 1 744 831 1077 1544 1567 1902 2148 3160
Size: 743 87 246 467 23 335 246 1012
Cuts at: 3160 3246 3258 3604 1
Size: 86 12 346 449
Fragments arranged by size:
1012 743 467 449 346 335 246 246
87 86 23 12
BtgI C'CryG_G
Cuts at: 107 168 657 107
Size: 61 489 3502
Fragments arranged by size:
3502 489 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2002 2183 2346 2002
Size: 181 163 3708
Fragments arranged by size:
3708 181 163
BtsI GCAGTG_nn'
Cuts at: 1295 1295
Size: 4052
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 475 510 475
Size: 35 4017
DraI TTT'AAA
Cuts at: 1051 1051
Size: 4052
DrdI GACnn_nn'nnGTC
Cuts at: 1758 2616 1758
Size: 858 3194
EaeI y'GGCC_r
Cuts at: 126 165 219 531 682 912 1637 1811 2202
Size: 39 54 312 151 230 725 174 391
Cuts at: 2202 2229 3574 126
Size: 27 1345 604
Fragments arranged by size:
1345 725 604 391 312 230 174 151
54 39 27
EagI C'GGCC_G
Cuts at: 912 1637 912
Size: 725 3327
EarI CTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2260 2570 2716 3862 2260
Size: 310 146 1146 2450
Fragments arranged by size:
2450 1146 310 146
EcoICRI GAG'CTC
Cuts at: 944 1064 944
Size: 120 3932
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 730 1877 2309 3056 3324 138
Size: 592 1147 432 747 268 866
Fragments arranged by size:
1147 866 747 592 432 268
EcoO109I rG'GnC_Cy
Cuts at: 735 1191 735
Size: 456 3596
EcoRI G'AATT_C
Cuts at: 1056 1056
Size: 4052
EcoRV GAT'ATC
Cuts at: 462 462
Size: 4052
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 4052
FspI TGC'GCA
Cuts at: 1833 1833
Size: 4052
HaeII r_GCGC'y
Cuts at: 203 657 1486 1734 2756 3735 203
Size: 454 829 248 1022 979 520
Fragments arranged by size:
1022 979 829 520 454 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 247 279 919 951 1894 1924 1926 1956 247
Size: 32 640 32 943 30 2 30 2343
Fragments arranged by size:
2343 943 640 32 32 30 30 2
HincII GTy'rAC
Cuts at: 378 1091 378
Size: 713 3339
KasI G'GCGC_C
Cuts at: 199 653 1482 1730 199
Size: 454 829 248 2521
Fragments arranged by size:
2521 829 454 248
KpnI G_GTAC'C
Cuts at: 526 1072 526
Size: 546 3506
MluI A'CGCG_T
Cuts at: 3485 3485
Size: 4052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 453 2723 2907 453
Size: 2270 184 1598
Fragments arranged by size:
2270 1598 184
MscI TGG'CCA
Cuts at: 128 533 1813 128
Size: 405 1280 2367
Fragments arranged by size:
2367 1280 405
MslI CAynn'nnrTG
Cuts at: 2041 2168 2041
Size: 127 3925
MspA1I CmG'CkG
Cuts at: 529 668 1163 1477 1837 2850 3095 529
Size: 139 495 314 360 1013 245 1486
Fragments arranged by size:
1486 1013 495 360 314 245 139
NaeI GCC'GGC
Cuts at: 2233 2233
Size: 4052
NarI GG'CG_CC
Cuts at: 200 654 1483 1731 200
Size: 454 829 248 2521
Fragments arranged by size:
2521 829 454 248
NcoI C'CATG_G
Cuts at: 107 657 107
Size: 550 3502
NdeI CA'TA_TG
Cuts at: 719 719
Size: 4052
NgoMIV G'CCGG_C
Cuts at: 2231 2231
Size: 4052
NspI r_CATG'y
Cuts at: 1105 1605 2512 1105
Size: 500 907 2645
Fragments arranged by size:
2645 907 500
PciI A'CATG_T
Cuts at: 2508 2508
Size: 4052
PmeI GTTT'AAAC
Cuts at: 1051 1051
Size: 4052
PmlI CAC'GTG
Cuts at: 960 960
Size: 4052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 4020
PpuMI rG'GwC_Cy
Cuts at: 735 735
Size: 4052
PstI C_TGCA'G
Cuts at: 575 743 1099 1784 575
Size: 168 356 685 2843
Fragments arranged by size:
2843 685 356 168
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4052
PvuII CAG'CTG
Cuts at: 529 668 1477 1837 529
Size: 139 809 360 2744
Fragments arranged by size:
2744 809 360 139
RsrII CG'GwC_CG
Cuts at: 2247 2247
Size: 4052
SacI G_AGCT'C
Cuts at: 946 1066 946
Size: 120 3932
SalI G'TCGA_C
Cuts at: 1089 1089
Size: 4052
SapI GCTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
SbfI CC_TGCA'GG
Cuts at: 743 1099 743
Size: 356 3696
ScaI AGT'ACT
Cuts at: 3569 3569
Size: 4052
SfcI C'TryA_G
Cuts at: 33 571 739 1095 1780 2773 2964 33
Size: 538 168 356 685 993 191 1121
Fragments arranged by size:
1121 993 685 538 356 191 168
SfoI GGC'GCC
Cuts at: 201 655 1484 1732 201
Size: 454 829 248 2521
Fragments arranged by size:
2521 829 454 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
SmaI CCC'GGG
Cuts at: 1074 1074
Size: 4052
SmlI C'TyrA_G
Cuts at: 1207 2122 2614 2876 1207
Size: 915 492 262 2383
Fragments arranged by size:
2383 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4052
SphI G_CATG'C
Cuts at: 1105 1605 1105
Size: 500 3552
StyI C'CwwG_G
Cuts at: 107 657 1186 1540 107
Size: 550 529 354 2619
Fragments arranged by size:
2619 550 529 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1635 1768 2227 2436 1635
Size: 133 459 209 3251
Fragments arranged by size:
3251 459 209 133
TatI w'GTAC_w
Cuts at: 3567 3567
Size: 4052
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4020
Tth111I GACn'n_nGTC
Cuts at: 1849 1849
Size: 4052
XbaI T'CTAG_A
Cuts at: 363 1083 363
Size: 720 3332
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1547 1547
Size: 4052
XmaI C'CCGG_G
Cuts at: 1072 1072
Size: 4052
XmnI GAAnn'nnTTC
Cuts at: 760 760
Size: 4052
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI