Restriction map for
pF1KB8332
(Circular) (Six-base) MAPSORT of: pF1KB8332.seq Check: 5664 from: 1 to: 4949
pF1KB8332 4949 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 802 802
Size: 4949
AccI GT'mk_AC
Cuts at: 467 496 1502 1987 467
Size: 29 1006 485 3429
Fragments arranged by size:
3429 1006 485 29
Acc65I G'GTAC_C
Cuts at: 1707 1965 1707
Size: 258 4691
AclI AA'CG_TT
Cuts at: 4789 4789
Size: 4949
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 308 611 677 1820 2774 3206 3953 4221 308
Size: 303 66 1143 954 432 747 268 1036
Fragments arranged by size:
1143 1036 954 747 432 303 268 66
AflIII A'CryG_T
Cuts at: 2301 3405 4382 2301
Size: 1104 977 2868
Fragments arranged by size:
2868 1104 977
AgeI A'CCGG_T
Cuts at: 3337 3337
Size: 4949
AlwNI CAG_nnn'CTG
Cuts at: 159 1162 1327 2254 3821 159
Size: 1003 165 927 1567 1287
Fragments arranged by size:
1567 1287 1003 927 165
ApaLI G'TGCA_C
Cuts at: 3719 3719
Size: 4949
ApoI r'AATT_y
Cuts at: 691 1054 1247 1953 4302 4558 691
Size: 363 193 706 2349 256 1082
Fragments arranged by size:
2349 1082 706 363 256 193
AseI AT'TA_AT
Cuts at: 20 1892 20
Size: 1872 3077
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4949
AvaI C'yCGr_G
Cuts at: 479 1969 479
Size: 1490 3459
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1691 1724 1691
Size: 33 4916
BamHI G'GATC_C
Cuts at: 1974 1974
Size: 4949
BanI G'GyrC_C
Cuts at: 409 1707 1965 2379 2627 2662 409
Size: 1298 258 414 248 35 2696
Fragments arranged by size:
2696 1298 414 258 248 35
BanII G_rGCy'C
Cuts at: 738 1963 2993 738
Size: 1225 1030 2694
Fragments arranged by size:
2694 1225 1030
BbeI G_GCGC'C
Cuts at: 413 2383 2631 413
Size: 1970 248 2731
Fragments arranged by size:
2731 1970 248
BbsI GAAGACnn'nnnn_
Cuts at: 794 794
Size: 4949
BciVI GTATCCnnnnn_n'
Cuts at: 911 2847 3608 4949 911
Size: 1936 761 1341 911
Fragments arranged by size:
1936 1341 911 761
BclI T'GATC_A
Cuts at: 2469 2469
Size: 4949
BglI GCCn_nnn'nGGC
Cuts at: 1415 1415
Size: 4949
BglII A'GATC_T
Cuts at: 261 2464 4501 261
Size: 2203 2037 709
Fragments arranged by size:
2203 2037 709
BlpI GC'TnA_GC
Cuts at: 2061 2061
Size: 4949
Bme1580I G_kGCm'C
Cuts at: 1866 2574 2667 3723 1866
Size: 708 93 1056 3092
Fragments arranged by size:
3092 1056 708 93
BmrI ACTGGGnnnn_n'
Cuts at: 2161 2242 2577 4721 2161
Size: 81 335 2144 2389
Fragments arranged by size:
2389 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3316 3316
Size: 4949
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 422 500 1118 302
Size: 120 78 618 4133
Fragments arranged by size:
4133 618 120 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2125 3004 3496 3794 2125
Size: 879 492 298 3280
Fragments arranged by size:
3280 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4653 4653
Size: 4949
BsaAI yAC'GTr
Cuts at: 494 2302 494
Size: 1808 3141
BsaBI GATnn'nnATC
Cuts at: 6 754 1228 2463 4500 6
Size: 748 474 1235 2037 455
Fragments arranged by size:
2037 1235 748 474 455
BsaHI Gr'CG_yC
Cuts at: 410 2380 2628 3330 4826 410
Size: 1970 248 702 1496 533
Fragments arranged by size:
1970 1496 702 533 248
BsaWI w'CCGG_w
Cuts at: 903 2138 2659 3337 3611 3758 4169 903
Size: 1235 521 678 274 147 411 1683
Fragments arranged by size:
1683 1235 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 498 528 498
Size: 30 4919
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 329 467 1930 329
Size: 138 1463 3348
Fragments arranged by size:
3348 1463 138
BseYI C'CCAG_C
Cuts at: 1638 2374 3132 3709 4205 1638
Size: 736 758 577 496 2382
Fragments arranged by size:
2382 758 736 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1148 1148
Size: 4949
BsiEI CG_ry'CG
Cuts at: 104 2491 2537 3745 104
Size: 2387 46 1208 1308
Fragments arranged by size:
2387 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 373 738 1963 2741 2931 3723 373
Size: 365 1225 778 190 792 1599
Fragments arranged by size:
1599 1225 792 778 365 190
Bsp1286I G_dGCh'C
Cuts at: 373 738 1866 1963 2574 2667 2741 2931 2993
Size: 365 1128 97 611 93 74 190 62
Cuts at: 2993 3723 373
Size: 730 1599
Fragments arranged by size:
1599 1128 730 611 365 190 97 93
74 62
BspEI T'CCGG_A
Cuts at: 903 4169 903
Size: 3266 1683
BspHI T'CATG_A
Cuts at: 645 948 4125 645
Size: 303 3177 1469
Fragments arranged by size:
3177 1469 303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 802 1947 1999 2213 2515 2896 802
Size: 1145 52 214 302 381 2855
Fragments arranged by size:
2855 1145 381 302 214 52
BsrBI CCG'CTC
Cuts at: 50 3241 3295 4779 4944 50
Size: 3191 54 1484 165 55
Fragments arranged by size:
3191 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 292 1857 2861 292
Size: 1565 1004 2380
Fragments arranged by size:
2380 1565 1004
BsrFI r'CCGG_y
Cuts at: 2947 3128 3337 2947
Size: 181 209 4559
Fragments arranged by size:
4559 209 181
BsrGI T'GTAC_A
Cuts at: 1397 1397
Size: 4949
BssSI C'ACGA_G
Cuts at: 913 3220 3578 913
Size: 2307 358 2284
Fragments arranged by size:
2307 2284 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2326 2326
Size: 4949
BstBI TT'CG_AA
Cuts at: 3310 3310
Size: 4949
BstEII G'GTnAC_C
Cuts at: 1625 1625
Size: 4949
BstXI CCAn_nnnn'nTGG
Cuts at: 400 400
Size: 4949
BstYI r'GATC_y
Cuts at: 1 261 418 1974 2441 2464 2799 3045 4057
Size: 260 157 1556 467 23 335 246 1012
Cuts at: 4057 4143 4155 4501 1
Size: 86 12 346 449
Fragments arranged by size:
1556 1012 467 449 346 335 260 246
157 86 23 12
BstZ17I GTA'TAC
Cuts at: 497 497
Size: 4949
BtgI C'CryG_G
Cuts at: 413 1413 413
Size: 1000 3949
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2899 3080 3243 2899
Size: 181 163 4605
Fragments arranged by size:
4605 181 163
BtsI GCAGTG_nn'
Cuts at: 1017 2192 1017
Size: 1175 3774
DraI TTT'AAA
Cuts at: 608 1948 608
Size: 1340 3609
DrdI GACnn_nn'nnGTC
Cuts at: 1338 2655 3513 1338
Size: 1317 858 2774
Fragments arranged by size:
2774 1317 858
EaeI y'GGCC_r
Cuts at: 1872 2534 2708 3099 3126 4471 1872
Size: 662 174 391 27 1345 2350
Fragments arranged by size:
2350 1345 662 391 174 27
EagI C'GGCC_G
Cuts at: 2534 2534
Size: 4949
EarI CTCTTCn'nnn_
Cuts at: 178 539 590 2972 3182 178
Size: 361 51 2382 210 1945
Fragments arranged by size:
2382 1945 361 210 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3157 3467 3613 4759 3157
Size: 310 146 1146 3347
Fragments arranged by size:
3347 1146 310 146
EcoICRI GAG'CTC
Cuts at: 736 1961 736
Size: 1225 3724
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 308 422 500 611 677 1118 1820 2774
Size: 6 114 78 111 66 441 702 954
Cuts at: 2774 3206 3953 4221 302
Size: 432 747 268 1030
Fragments arranged by size:
1030 954 747 702 441 432 268 114
111 78 66 6
EcoO109I rG'GnC_Cy
Cuts at: 430 2088 430
Size: 1658 3291
EcoRI G'AATT_C
Cuts at: 1953 1953
Size: 4949
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 313 2968 313
Size: 2655 2294
FspI TGC'GCA
Cuts at: 2730 2730
Size: 4949
HaeII r_GCGC'y
Cuts at: 413 2383 2631 3653 4632 413
Size: 1970 248 1022 979 730
Fragments arranged by size:
1970 1022 979 730 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 473 505 841 873 877 909 2791 2821 2823
Size: 32 336 32 4 32 1882 30 2
Cuts at: 2823 2853 473
Size: 30 2569
Fragments arranged by size:
2569 1882 336 32 32 32 30 30
4 2
HincII GTy'rAC
Cuts at: 468 1362 1988 468
Size: 894 626 3429
Fragments arranged by size:
3429 894 626
HindIII A'AGCT_T
Cuts at: 1549 1549
Size: 4949
KasI G'GCGC_C
Cuts at: 409 2379 2627 409
Size: 1970 248 2731
Fragments arranged by size:
2731 1970 248
KpnI G_GTAC'C
Cuts at: 1711 1969 1711
Size: 258 4691
MluI A'CGCG_T
Cuts at: 4382 4382
Size: 4949
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 119 1179 1332 1473 3620 3804 119
Size: 1060 153 141 2147 184 1264
Fragments arranged by size:
2147 1264 1060 184 153 141
MscI TGG'CCA
Cuts at: 2710 2710
Size: 4949
MslI CAynn'nnrTG
Cuts at: 1481 1726 2938 3065 1481
Size: 245 1212 127 3365
Fragments arranged by size:
3365 1212 245 127
MspA1I CmG'CkG
Cuts at: 1030 1162 1489 2060 2374 2734 3747 3992 1030
Size: 132 327 571 314 360 1013 245 1987
Fragments arranged by size:
1987 1013 571 360 327 314 245 132
NaeI GCC'GGC
Cuts at: 3130 3130
Size: 4949
NarI GG'CG_CC
Cuts at: 410 2380 2628 410
Size: 1970 248 2731
Fragments arranged by size:
2731 1970 248
NcoI C'CATG_G
Cuts at: 1413 1413
Size: 4949
NgoMIV G'CCGG_C
Cuts at: 3128 3128
Size: 4949
NspI r_CATG'y
Cuts at: 1272 1409 2002 2502 3409 1272
Size: 137 593 500 907 2812
Fragments arranged by size:
2812 907 593 500 137
PciI A'CATG_T
Cuts at: 3405 3405
Size: 4949
PflMI CCAn_nnn'nTGG
Cuts at: 1468 1468
Size: 4949
PmeI GTTT'AAAC
Cuts at: 1948 1948
Size: 4949
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4088 4120 4088
Size: 32 4917
PpuMI rG'GwC_Cy
Cuts at: 430 430
Size: 4949
PstI C_TGCA'G
Cuts at: 1996 2681 1996
Size: 685 4264
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4949
PvuII CAG'CTG
Cuts at: 1030 1162 1489 2374 2734 1030
Size: 132 327 885 360 3245
Fragments arranged by size:
3245 885 360 327 132
RsrII CG'GwC_CG
Cuts at: 3144 3144
Size: 4949
SacI G_AGCT'C
Cuts at: 738 1963 738
Size: 1225 3724
SalI G'TCGA_C
Cuts at: 466 1986 466
Size: 1520 3429
SapI GCTCTTCn'nnn_
Cuts at: 2972 3182 2972
Size: 210 4739
SbfI CC_TGCA'GG
Cuts at: 1996 1996
Size: 4949
ScaI AGT'ACT
Cuts at: 4466 4466
Size: 4949
SfcI C'TryA_G
Cuts at: 33 768 1992 2677 3670 3861 33
Size: 735 1224 685 993 191 1121
Fragments arranged by size:
1224 1121 993 735 685 191
SfoI GGC'GCC
Cuts at: 411 2381 2629 411
Size: 1970 248 2731
Fragments arranged by size:
2731 1970 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4949
SmaI CCC'GGG
Cuts at: 1971 1971
Size: 4949
SmlI C'TyrA_G
Cuts at: 479 2104 3019 3511 3773 479
Size: 1625 915 492 262 1655
Fragments arranged by size:
1655 1625 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4949
SphI G_CATG'C
Cuts at: 1409 2002 2502 1409
Size: 593 500 3856
Fragments arranged by size:
3856 593 500
StyI C'CwwG_G
Cuts at: 1413 2083 2437 1413
Size: 670 354 3925
Fragments arranged by size:
3925 670 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1652 1780 2532 2665 3124 3333 1652
Size: 128 752 133 459 209 3268
Fragments arranged by size:
3268 752 459 209 133 128
TatI w'GTAC_w
Cuts at: 1397 4464 1397
Size: 3067 1882
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4466 4498 4466
Size: 32 4917
Tth111I GACn'n_nGTC
Cuts at: 2746 2746
Size: 4949
XbaI T'CTAG_A
Cuts at: 421 1980 421
Size: 1559 3390
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1725 2444 1725
Size: 719 4230
XhoI C'TCGA_G
Cuts at: 479 479
Size: 4949
XmaI C'CCGG_G
Cuts at: 1969 1969
Size: 4949
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BssHII Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI