Restriction map for
pF1KB8331
(Circular) (Six-base) MAPSORT of: pF1KB8331.seq Check: 943 from: 1 to: 3743
pF1KB8331 3743 bp
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
AccI GT'mk_AC
Cuts at: 781 781
Size: 3743
Acc65I G'GTAC_C
Cuts at: 759 759
Size: 3743
AclI AA'CG_TT
Cuts at: 3583 3583
Size: 3743
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 1568 2000 2747 3015 496
Size: 1072 432 747 268 1224
Fragments arranged by size:
1224 1072 747 432 268
AflIII A'CryG_T
Cuts at: 1095 2199 3176 1095
Size: 1104 977 1662
Fragments arranged by size:
1662 1104 977
AgeI A'CCGG_T
Cuts at: 2131 2131
Size: 3743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 323 323
Size: 3743
AlwNI CAG_nnn'CTG
Cuts at: 1048 2615 1048
Size: 1567 2176
ApaLI G'TGCA_C
Cuts at: 2513 2513
Size: 3743
ApoI r'AATT_y
Cuts at: 747 3096 3352 747
Size: 2349 256 1138
Fragments arranged by size:
2349 1138 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3743
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3743
AvaI C'yCGr_G
Cuts at: 242 267 437 670 763 242
Size: 25 170 233 93 3222
Fragments arranged by size:
3222 233 170 93 25
BamHI G'GATC_C
Cuts at: 428 574 768 428
Size: 146 194 3403
Fragments arranged by size:
3403 194 146
BanI G'GyrC_C
Cuts at: 220 283 307 332 759 1173 1421 1456 220
Size: 63 24 25 427 414 248 35 2507
Fragments arranged by size:
2507 427 414 248 63 35 25 24
BanII G_rGCy'C
Cuts at: 681 757 1787 681
Size: 76 1030 2637
Fragments arranged by size:
2637 1030 76
BbeI G_GCGC'C
Cuts at: 287 1177 1425 287
Size: 890 248 2605
Fragments arranged by size:
2605 890 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 248 282 684 718 90
Size: 34 124 34 402 34 3115
Fragments arranged by size:
3115 402 124 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1641 2402 3743 1641
Size: 761 1341 1641
Fragments arranged by size:
1641 1341 761
BclI T'GATC_A
Cuts at: 1263 1263
Size: 3743
BglI GCCn_nnn'nGGC
Cuts at: 112 164 191 112
Size: 52 27 3664
Fragments arranged by size:
3664 52 27
BglII A'GATC_T
Cuts at: 606 1258 3295 606
Size: 652 2037 1054
Fragments arranged by size:
2037 1054 652
BlpI GC'TnA_GC
Cuts at: 855 855
Size: 3743
Bme1580I G_kGCm'C
Cuts at: 130 337 1368 1461 2517 130
Size: 207 1031 93 1056 1356
Fragments arranged by size:
1356 1056 1031 207 93
BmrI ACTGGGnnnn_n'
Cuts at: 955 1036 1371 3515 955
Size: 81 335 2144 1183
Fragments arranged by size:
2144 1183 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 3743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 919 1798 2290 2588 391
Size: 528 879 492 298 1546
Fragments arranged by size:
1546 879 528 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 636 3447 636
Size: 2811 932
BsaAI yAC'GTr
Cuts at: 1096 1096
Size: 3743
BsaBI GATnn'nnATC
Cuts at: 6 1257 3294 6
Size: 1251 2037 455
Fragments arranged by size:
2037 1251 455
BsaHI Gr'CG_yC
Cuts at: 284 1174 1422 2124 3620 284
Size: 890 248 702 1496 407
Fragments arranged by size:
1496 890 702 407 248
BsaWI w'CCGG_w
Cuts at: 932 1453 2131 2405 2552 2963 932
Size: 521 678 274 147 411 1712
Fragments arranged by size:
1712 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 293 293
Size: 3743
BseYI C'CCAG_C
Cuts at: 541 570 1168 1926 2503 2999 541
Size: 29 598 758 577 496 1285
Fragments arranged by size:
1285 758 598 577 496 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 714 714
Size: 3743
BsiEI CG_ry'CG
Cuts at: 104 1285 1331 2539 104
Size: 1181 46 1208 1308
Fragments arranged by size:
1308 1208 1181 46
BsiHKAI G_wGCw'C
Cuts at: 757 1535 1725 2517 757
Size: 778 190 792 1983
Fragments arranged by size:
1983 792 778 190
BsmI GAATG_Cn'
Cuts at: 173 173
Size: 3743
Bsp1286I G_dGCh'C
Cuts at: 130 337 681 757 1368 1461 1535 1725 1787
Size: 207 344 76 611 93 74 190 62
Cuts at: 1787 2517 130
Size: 730 1356
Fragments arranged by size:
1356 730 611 344 207 190 93 76
74 62
BspEI T'CCGG_A
Cuts at: 2963 2963
Size: 3743
BspHI T'CATG_A
Cuts at: 2919 2919
Size: 3743
BspMI ACCTGCnnnn'nnnn_
Cuts at: 793 1007 1309 1690 793
Size: 214 302 381 2846
Fragments arranged by size:
2846 381 302 214
BsrBI CCG'CTC
Cuts at: 50 453 2035 2089 3573 3738 50
Size: 403 1582 54 1484 165 55
Fragments arranged by size:
1582 1484 403 165 55 54
BsrDI GCAATG_nn'
Cuts at: 201 1655 201
Size: 1454 2289
BsrFI r'CCGG_y
Cuts at: 1741 1922 2131 1741
Size: 181 209 3353
Fragments arranged by size:
3353 209 181
BssSI C'ACGA_G
Cuts at: 2014 2372 2014
Size: 358 3385
BstAPI GCAn_nnn'nTGC
Cuts at: 1120 1120
Size: 3743
BstBI TT'CG_AA
Cuts at: 2104 2104
Size: 3743
BstXI CCAn_nnnn'nTGG
Cuts at: 688 688
Size: 3743
BstYI r'GATC_y
Cuts at: 1 428 574 606 768 1235 1258 1593 1839
Size: 427 146 32 162 467 23 335 246
Cuts at: 1839 2851 2937 2949 3295 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 427 346 335 246 162
146 86 32 23 12
BtgI C'CryG_G
Cuts at: 224 289 404 224
Size: 65 115 3563
Fragments arranged by size:
3563 115 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 161 1693 1874 2037 161
Size: 1532 181 163 1867
Fragments arranged by size:
1867 1532 181 163
BtsI GCAGTG_nn'
Cuts at: 986 986
Size: 3743
DraI TTT'AAA
Cuts at: 742 742
Size: 3743
DrdI GACnn_nn'nnGTC
Cuts at: 1449 2307 1449
Size: 858 2885
EaeI y'GGCC_r
Cuts at: 1328 1502 1893 1920 3265 1328
Size: 174 391 27 1345 1806
Fragments arranged by size:
1806 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1328 1328
Size: 3743
EarI CTCTTCn'nnn_
Cuts at: 1766 1976 1766
Size: 210 3533
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 561 567 1951 2261 2407 3553 561
Size: 6 1384 310 146 1146 751
Fragments arranged by size:
1384 1146 751 310 146 6
EcoICRI GAG'CTC
Cuts at: 755 755
Size: 3743
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 1568 2000 2747 3015 496
Size: 1072 432 747 268 1224
Fragments arranged by size:
1224 1072 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 166 238 263 882 166
Size: 72 25 619 3027
Fragments arranged by size:
3027 619 72 25
EcoRI G'AATT_C
Cuts at: 747 747
Size: 3743
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1762 1762
Size: 3743
FspI TGC'GCA
Cuts at: 320 1524 320
Size: 1204 2539
HaeII r_GCGC'y
Cuts at: 287 542 1177 1425 2447 3426 287
Size: 255 635 248 1022 979 604
Fragments arranged by size:
1022 979 635 604 255 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1585 1615 1617 1647 1585
Size: 30 2 30 3681
Fragments arranged by size:
3681 30 30 2
HincII GTy'rAC
Cuts at: 782 782
Size: 3743
KasI G'GCGC_C
Cuts at: 283 1173 1421 283
Size: 890 248 2605
Fragments arranged by size:
2605 890 248
KpnI G_GTAC'C
Cuts at: 763 763
Size: 3743
MluI A'CGCG_T
Cuts at: 3176 3176
Size: 3743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2414 2598 2414
Size: 184 3559
MscI TGG'CCA
Cuts at: 1504 1504
Size: 3743
MslI CAynn'nnrTG
Cuts at: 305 385 1732 1859 305
Size: 80 1347 127 2189
Fragments arranged by size:
2189 1347 127 80
MspA1I CmG'CkG
Cuts at: 291 854 1168 1528 2541 2786 291
Size: 563 314 360 1013 245 1248
Fragments arranged by size:
1248 1013 563 360 314 245
NaeI GCC'GGC
Cuts at: 1924 1924
Size: 3743
NarI GG'CG_CC
Cuts at: 284 1174 1422 284
Size: 890 248 2605
Fragments arranged by size:
2605 890 248
NcoI C'CATG_G
Cuts at: 404 404
Size: 3743
NgoMIV G'CCGG_C
Cuts at: 1922 1922
Size: 3743
NspI r_CATG'y
Cuts at: 796 1296 2203 796
Size: 500 907 2336
Fragments arranged by size:
2336 907 500
PasI CC'CwG_GG
Cuts at: 187 681 187
Size: 494 3249
PciI A'CATG_T
Cuts at: 2199 2199
Size: 3743
PmeI GTTT'AAAC
Cuts at: 742 742
Size: 3743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2882 2914 2882
Size: 32 3711
PpuMI rG'GwC_Cy
Cuts at: 238 238
Size: 3743
PstI C_TGCA'G
Cuts at: 790 1475 790
Size: 685 3058
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3743
PvuII CAG'CTG
Cuts at: 1168 1528 1168
Size: 360 3383
RsrII CG'GwC_CG
Cuts at: 1938 1938
Size: 3743
SacI G_AGCT'C
Cuts at: 757 757
Size: 3743
SacII CC_GC'GG
Cuts at: 292 292
Size: 3743
SalI G'TCGA_C
Cuts at: 780 780
Size: 3743
SapI GCTCTTCn'nnn_
Cuts at: 1766 1976 1766
Size: 210 3533
SbfI CC_TGCA'GG
Cuts at: 790 790
Size: 3743
ScaI AGT'ACT
Cuts at: 3260 3260
Size: 3743
SexAI A'CCwGG_T
Cuts at: 645 645
Size: 3743
SfcI C'TryA_G
Cuts at: 33 786 1471 2464 2655 33
Size: 753 685 993 191 1121
Fragments arranged by size:
1121 993 753 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 164 164
Size: 3743
SfoI GGC'GCC
Cuts at: 285 1175 1423 285
Size: 890 248 2605
Fragments arranged by size:
2605 890 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3743
SmaI CCC'GGG
Cuts at: 244 439 765 244
Size: 195 326 3222
Fragments arranged by size:
3222 326 195
SmlI C'TyrA_G
Cuts at: 370 898 1813 2305 2567 370
Size: 528 915 492 262 1546
Fragments arranged by size:
1546 915 528 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3743
SphI G_CATG'C
Cuts at: 796 1296 796
Size: 500 3243
StuI AGG'CCT
Cuts at: 631 631
Size: 3743
StyI C'CwwG_G
Cuts at: 404 877 1231 404
Size: 473 354 2916
Fragments arranged by size:
2916 473 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 633 680 1326 1459 1918 2127 633
Size: 47 646 133 459 209 2249
Fragments arranged by size:
2249 646 459 209 133 47
TatI w'GTAC_w
Cuts at: 3258 3258
Size: 3743
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 316 348 3260 3292 316
Size: 32 2912 32 767
Fragments arranged by size:
2912 767 32 32
Tth111I GACn'n_nGTC
Cuts at: 1540 1540
Size: 3743
XbaI T'CTAG_A
Cuts at: 774 774
Size: 3743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1238 1238
Size: 3743
XmaI C'CCGG_G
Cuts at: 242 437 763 242
Size: 195 326 3222
Fragments arranged by size:
3222 326 195
XmnI GAAnn'nnTTC
Cuts at: 481 481
Size: 3743
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI
BpmI Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SanDI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI