Restriction map for
pF1KB8326
(Circular) (Six-base) MAPSORT of: pF1KB8326.seq Check: 1188 from: 1 to: 4211
pF1KB8326 4211 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 351 695 351
Size: 344 3867
AccI GT'mk_AC
Cuts at: 1249 1249
Size: 4211
Acc65I G'GTAC_C
Cuts at: 1227 1227
Size: 4211
AclI AA'CG_TT
Cuts at: 4051 4051
Size: 4211
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2036 2468 3215 3483 2036
Size: 432 747 268 2764
Fragments arranged by size:
2764 747 432 268
AflIII A'CryG_T
Cuts at: 1563 2667 3644 1563
Size: 1104 977 2130
Fragments arranged by size:
2130 1104 977
AgeI A'CCGG_T
Cuts at: 2599 2599
Size: 4211
AlwNI CAG_nnn'CTG
Cuts at: 126 279 459 1516 3083 126
Size: 153 180 1057 1567 1254
Fragments arranged by size:
1567 1254 1057 180 153
ApaI G_GGCC'C
Cuts at: 777 777
Size: 4211
ApaLI G'TGCA_C
Cuts at: 682 2981 682
Size: 2299 1912
ApoI r'AATT_y
Cuts at: 1215 3564 3820 1215
Size: 2349 256 1606
Fragments arranged by size:
2349 1606 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4211
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4211
AvaI C'yCGr_G
Cuts at: 673 1231 673
Size: 558 3653
BamHI G'GATC_C
Cuts at: 1236 1236
Size: 4211
BanI G'GyrC_C
Cuts at: 436 598 696 1020 1227 1641 1889 1924 436
Size: 162 98 324 207 414 248 35 2723
Fragments arranged by size:
2723 414 324 248 207 162 98 35
BanII G_rGCy'C
Cuts at: 777 1013 1088 1199 1225 2255 777
Size: 236 75 111 26 1030 2733
Fragments arranged by size:
2733 1030 236 111 75 26
BbeI G_GCGC'C
Cuts at: 1645 1893 1645
Size: 248 3963
BbsI GAAGACnn'nnnn_
Cuts at: 725 725
Size: 4211
BbvCI CC'TCA_GC
Cuts at: 574 574
Size: 4211
BciVI GTATCCnnnnn_n'
Cuts at: 2109 2870 4211 2109
Size: 761 1341 2109
Fragments arranged by size:
2109 1341 761
BclI T'GATC_A
Cuts at: 1731 1731
Size: 4211
BfrBI ATG'CAT
Cuts at: 405 405
Size: 4211
BglI GCCn_nnn'nGGC
Cuts at: 289 789 289
Size: 500 3711
BglII A'GATC_T
Cuts at: 1726 3763 1726
Size: 2037 2174
BlpI GC'TnA_GC
Cuts at: 1323 1323
Size: 4211
Bme1580I G_kGCm'C
Cuts at: 183 320 686 701 777 1105 1836 1929 2985
Size: 137 366 15 76 328 731 93 1056
Cuts at: 2985 183
Size: 1409
Fragments arranged by size:
1409 1056 731 366 328 137 93 76
15
BmrI ACTGGGnnnn_n'
Cuts at: 1423 1504 1839 3983 1423
Size: 81 335 2144 1651
Fragments arranged by size:
2144 1651 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2578 2578
Size: 4211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 788 904 268
Size: 520 116 3575
Fragments arranged by size:
3575 520 116
Bpu10I CC'TnA_GC
Cuts at: 441 574 441
Size: 133 4078
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1387 2266 2758 3056 1387
Size: 879 492 298 2542
Fragments arranged by size:
2542 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 247 516 651 3915 247
Size: 269 135 3264 543
Fragments arranged by size:
3264 543 269 135
BsaAI yAC'GTr
Cuts at: 1564 1564
Size: 4211
BsaBI GATnn'nnATC
Cuts at: 6 1725 3762 6
Size: 1719 2037 455
Fragments arranged by size:
2037 1719 455
BsaHI Gr'CG_yC
Cuts at: 1151 1642 1890 2592 4088 1151
Size: 491 248 702 1496 1274
Fragments arranged by size:
1496 1274 702 491 248
BsaWI w'CCGG_w
Cuts at: 906 1400 1921 2599 2873 3020 3431 906
Size: 494 521 678 274 147 411 1686
Fragments arranged by size:
1686 678 521 494 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 877 907 877
Size: 30 4181
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 265 714 830 901 932 935 265
Size: 449 116 71 31 3 3541
Fragments arranged by size:
3541 449 116 71 31 3
BseYI C'CCAG_C
Cuts at: 384 820 1012 1016 1198 1636 2394 2971 3467
Size: 436 192 4 182 438 758 577 496
Cuts at: 3467 384
Size: 1128
Fragments arranged by size:
1128 758 577 496 438 436 192 182
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 241
Size: 4211
BsiEI CG_ry'CG
Cuts at: 104 749 1753 1799 3007 104
Size: 645 1004 46 1208 1308
Fragments arranged by size:
1308 1208 1004 645 46
BsiHKAI G_wGCw'C
Cuts at: 686 1088 1225 2003 2193 2985 686
Size: 402 137 778 190 792 1912
Fragments arranged by size:
1912 792 778 402 190 137
Bsp1286I G_dGCh'C
Cuts at: 183 320 686 701 777 1013 1088 1105 1199
Size: 137 366 15 76 236 75 17 94
Cuts at: 1199 1225 1836 1929 2003 2193 2255 2985 183
Size: 26 611 93 74 190 62 730 1409
Fragments arranged by size:
1409 730 611 366 236 190 137 94
93 76 75 74 62 26 17 15
BspEI T'CCGG_A
Cuts at: 3431 3431
Size: 4211
BspHI T'CATG_A
Cuts at: 3387 3387
Size: 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 347 351 695 944 1261 1475 1777 2158 347
Size: 4 344 249 317 214 302 381 2400
Fragments arranged by size:
2400 381 344 317 302 249 214 4
BsrBI CCG'CTC
Cuts at: 50 2503 2557 4041 4206 50
Size: 2453 54 1484 165 55
Fragments arranged by size:
2453 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2123 2123
Size: 4211
BsrFI r'CCGG_y
Cuts at: 690 748 2209 2390 2599 690
Size: 58 1461 181 209 2302
Fragments arranged by size:
2302 1461 209 181 58
BssSI C'ACGA_G
Cuts at: 2482 2840 2482
Size: 358 3853
BstAPI GCAn_nnn'nTGC
Cuts at: 324 1588 324
Size: 1264 2947
BstBI TT'CG_AA
Cuts at: 2572 2572
Size: 4211
BstXI CCAn_nnnn'nTGG
Cuts at: 828 828
Size: 4211
BstYI r'GATC_y
Cuts at: 1 140 1236 1703 1726 2061 2307 3319 3405
Size: 139 1096 467 23 335 246 1012 86
Cuts at: 3405 3417 3763 1
Size: 12 346 449
Fragments arranged by size:
1096 1012 467 449 346 335 246 139
86 23 12
BtgI C'CryG_G
Cuts at: 107 257 558 107
Size: 150 301 3760
Fragments arranged by size:
3760 301 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 465 2161 2342 2505 465
Size: 1696 181 163 2171
Fragments arranged by size:
2171 1696 181 163
BtsI GCAGTG_nn'
Cuts at: 1454 1454
Size: 4211
DraI TTT'AAA
Cuts at: 1210 1210
Size: 4211
DrdI GACnn_nn'nnGTC
Cuts at: 1917 2775 1917
Size: 858 3353
EaeI y'GGCC_r
Cuts at: 150 281 311 468 555 746 1796 1970 2361
Size: 131 30 157 87 191 1050 174 391
Cuts at: 2361 2388 3733 150
Size: 27 1345 628
Fragments arranged by size:
1345 1050 628 391 191 174 157 131
87 30 27
EagI C'GGCC_G
Cuts at: 746 1796 746
Size: 1050 3161
EarI CTCTTCn'nnn_
Cuts at: 908 2234 2444 908
Size: 1326 210 2675
Fragments arranged by size:
2675 1326 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 633 705 2419 2729 2875 4021 127
Size: 506 72 1714 310 146 1146 317
Fragments arranged by size:
1714 1146 506 317 310 146 72
EcoICRI GAG'CTC
Cuts at: 1086 1223 1086
Size: 137 4074
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 788 904 2036 2468 3215 3483 268
Size: 520 116 1132 432 747 268 996
Fragments arranged by size:
1132 996 747 520 432 268 116
EcoNI CCTnn'n_nnAGG
Cuts at: 358 516 358
Size: 158 4053
EcoO109I rG'GnC_Cy
Cuts at: 570 624 773 774 1350 570
Size: 54 149 1 576 3431
Fragments arranged by size:
3431 576 149 54 1
EcoRI G'AATT_C
Cuts at: 1215 1215
Size: 4211
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2230 2230
Size: 4211
FspI TGC'GCA
Cuts at: 328 1992 328
Size: 1664 2547
HaeII r_GCGC'y
Cuts at: 247 758 1645 1893 2915 3894 247
Size: 511 887 248 1022 979 564
Fragments arranged by size:
1022 979 887 564 511 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 877 909 2053 2083 2085 2115 877
Size: 32 1144 30 2 30 2973
Fragments arranged by size:
2973 1144 32 30 30 2
HincII GTy'rAC
Cuts at: 1250 1250
Size: 4211
KasI G'GCGC_C
Cuts at: 1641 1889 1641
Size: 248 3963
KpnI G_GTAC'C
Cuts at: 1231 1231
Size: 4211
MluI A'CGCG_T
Cuts at: 3644 3644
Size: 4211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 314 2882 3066 314
Size: 2568 184 1459
Fragments arranged by size:
2568 1459 184
MscI TGG'CCA
Cuts at: 283 557 1972 283
Size: 274 1415 2522
Fragments arranged by size:
2522 1415 274
MslI CAynn'nnrTG
Cuts at: 406 2200 2327 406
Size: 1794 127 2290
Fragments arranged by size:
2290 1794 127
MspA1I CmG'CkG
Cuts at: 126 456 585 870 1016 1202 1322 1636 1996
Size: 330 129 285 146 186 120 314 360
Cuts at: 1996 3009 3254 126
Size: 1013 245 1083
Fragments arranged by size:
1083 1013 360 330 314 285 245 186
146 129 120
NaeI GCC'GGC
Cuts at: 750 2392 750
Size: 1642 2569
NarI GG'CG_CC
Cuts at: 1642 1890 1642
Size: 248 3963
NcoI C'CATG_G
Cuts at: 107 257 558 107
Size: 150 301 3760
Fragments arranged by size:
3760 301 150
NdeI CA'TA_TG
Cuts at: 407 407
Size: 4211
NgoMIV G'CCGG_C
Cuts at: 748 2390 748
Size: 1642 2569
NsiI A_TGCA'T
Cuts at: 407 407
Size: 4211
NspI r_CATG'y
Cuts at: 333 405 1264 1764 2671 333
Size: 72 859 500 907 1873
Fragments arranged by size:
1873 907 859 500 72
PasI CC'CwG_GG
Cuts at: 1180 1180
Size: 4211
PciI A'CATG_T
Cuts at: 2667 2667
Size: 4211
PflMI CCAn_nnn'nTGG
Cuts at: 279 997 279
Size: 718 3493
PmeI GTTT'AAAC
Cuts at: 1210 1210
Size: 4211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3350 3382 3350
Size: 32 4179
PpuMI rG'GwC_Cy
Cuts at: 570 570
Size: 4211
PspOMI G'GGCC_C
Cuts at: 773 773
Size: 4211
PstI C_TGCA'G
Cuts at: 131 344 520 1258 1943 131
Size: 213 176 738 685 2399
Fragments arranged by size:
2399 738 685 213 176
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4211
PvuII CAG'CTG
Cuts at: 126 585 1016 1202 1636 1996 126
Size: 459 431 186 434 360 2341
Fragments arranged by size:
2341 459 434 431 360 186
RsrII CG'GwC_CG
Cuts at: 2406 2406
Size: 4211
SacI G_AGCT'C
Cuts at: 1088 1225 1088
Size: 137 4074
SalI G'TCGA_C
Cuts at: 1248 1248
Size: 4211
SapI GCTCTTCn'nnn_
Cuts at: 908 2234 2444 908
Size: 1326 210 2675
Fragments arranged by size:
2675 1326 210
SbfI CC_TGCA'GG
Cuts at: 344 1258 344
Size: 914 3297
ScaI AGT'ACT
Cuts at: 3728 3728
Size: 4211
SexAI A'CCwGG_T
Cuts at: 296 296
Size: 4211
SfcI C'TryA_G
Cuts at: 33 118 127 340 516 1254 1939 2932 3123
Size: 85 9 213 176 738 685 993 191
Cuts at: 3123 33
Size: 1121
Fragments arranged by size:
1121 993 738 685 213 191 176 85
9
SfiI GGCCn_nnn'nGGCC
Cuts at: 289 289
Size: 4211
SfoI GGC'GCC
Cuts at: 1643 1891 1643
Size: 248 3963
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4211
SmaI CCC'GGG
Cuts at: 1233 1233
Size: 4211
SmlI C'TyrA_G
Cuts at: 1366 2281 2773 3035 1366
Size: 915 492 262 2542
Fragments arranged by size:
2542 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4211
SphI G_CATG'C
Cuts at: 1264 1764 1264
Size: 500 3711
StyI C'CwwG_G
Cuts at: 107 257 558 650 785 827 1345 1699 107
Size: 150 301 92 135 42 518 354 2619
Fragments arranged by size:
2619 518 354 301 150 135 92 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1008 1794 1927 2386 2595 1008
Size: 786 133 459 209 2624
Fragments arranged by size:
2624 786 459 209 133
TatI w'GTAC_w
Cuts at: 3726 3726
Size: 4211
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3728 3760 3728
Size: 32 4179
Tth111I GACn'n_nGTC
Cuts at: 2008 2008
Size: 4211
XbaI T'CTAG_A
Cuts at: 1242 1242
Size: 4211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1706 1706
Size: 4211
XmaI C'CCGG_G
Cuts at: 1231 1231
Size: 4211
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI PacI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI