Restriction map for
pF1KB8325
(Circular) (Six-base) MAPSORT of: pF1KB8325.seq Check: 9535 from: 1 to: 3731
pF1KB8325 3731 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 769 769
Size: 3731
Acc65I G'GTAC_C
Cuts at: 747 747
Size: 3731
AclI AA'CG_TT
Cuts at: 3571 3571
Size: 3731
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 1556 1988 2735 3003 154
Size: 1402 432 747 268 882
Fragments arranged by size:
1402 882 747 432 268
AflIII A'CryG_T
Cuts at: 1083 2187 3164 1083
Size: 1104 977 1650
Fragments arranged by size:
1650 1104 977
AgeI A'CCGG_T
Cuts at: 2119 2119
Size: 3731
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 312 344 312
Size: 32 3699
AlwNI CAG_nnn'CTG
Cuts at: 1036 2603 1036
Size: 1567 2164
ApaLI G'TGCA_C
Cuts at: 347 2501 347
Size: 2154 1577
ApoI r'AATT_y
Cuts at: 520 735 3084 3340 520
Size: 215 2349 256 911
Fragments arranged by size:
2349 911 256 215
AseI AT'TA_AT
Cuts at: 20 20
Size: 3731
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3731
AvaI C'yCGr_G
Cuts at: 751 751
Size: 3731
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 380 413 380
Size: 33 3698
BamHI G'GATC_C
Cuts at: 756 756
Size: 3731
BanI G'GyrC_C
Cuts at: 747 1161 1409 1444 747
Size: 414 248 35 3034
Fragments arranged by size:
3034 414 248 35
BanII G_rGCy'C
Cuts at: 745 1775 745
Size: 1030 2701
BbeI G_GCGC'C
Cuts at: 1165 1413 1165
Size: 248 3483
BciVI GTATCCnnnnn_n'
Cuts at: 288 1629 2390 3731 288
Size: 1341 761 1341 288
Fragments arranged by size:
1341 1341 761 288
BclI T'GATC_A
Cuts at: 1251 1251
Size: 3731
BglII A'GATC_T
Cuts at: 1246 3283 1246
Size: 2037 1694
BlpI GC'TnA_GC
Cuts at: 843 843
Size: 3731
Bme1580I G_kGCm'C
Cuts at: 351 1356 1449 2505 351
Size: 1005 93 1056 1577
Fragments arranged by size:
1577 1056 1005 93
BmgBI CAC'GTC
Cuts at: 120 120
Size: 3731
BmrI ACTGGGnnnn_n'
Cuts at: 943 1024 1359 3503 943
Size: 81 335 2144 1171
Fragments arranged by size:
2144 1171 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 3731
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 907 1786 2278 2576 204
Size: 703 879 492 298 1359
Fragments arranged by size:
1359 879 703 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3435 3435
Size: 3731
BsaAI yAC'GTr
Cuts at: 1084 1084
Size: 3731
BsaBI GATnn'nnATC
Cuts at: 6 142 1245 3282 6
Size: 136 1103 2037 455
Fragments arranged by size:
2037 1103 455 136
BsaHI Gr'CG_yC
Cuts at: 1162 1410 2112 3608 1162
Size: 248 702 1496 1285
Fragments arranged by size:
1496 1285 702 248
BsaWI w'CCGG_w
Cuts at: 920 1441 2119 2393 2540 2951 920
Size: 521 678 274 147 411 1700
Fragments arranged by size:
1700 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 312 342 312
Size: 30 3701
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 707 710 707
Size: 3 3728
BseYI C'CCAG_C
Cuts at: 331 1156 1914 2491 2987 331
Size: 825 758 577 496 1075
Fragments arranged by size:
1075 825 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1273 1319 2527 104
Size: 1169 46 1208 1308
Fragments arranged by size:
1308 1208 1169 46
BsiHKAI G_wGCw'C
Cuts at: 351 745 1523 1713 2505 351
Size: 394 778 190 792 1577
Fragments arranged by size:
1577 792 778 394 190
BsmI GAATG_Cn'
Cuts at: 534 534
Size: 3731
Bsp1286I G_dGCh'C
Cuts at: 351 745 1356 1449 1523 1713 1775 2505 351
Size: 394 611 93 74 190 62 730 1577
Fragments arranged by size:
1577 730 611 394 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2951 2951
Size: 3731
BspHI T'CATG_A
Cuts at: 2907 2907
Size: 3731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 781 995 1297 1678 781
Size: 214 302 381 2834
Fragments arranged by size:
2834 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2023 2077 3561 3726 50
Size: 1973 54 1484 165 55
Fragments arranged by size:
1973 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1643 1643
Size: 3731
BsrFI r'CCGG_y
Cuts at: 1729 1910 2119 1729
Size: 181 209 3341
Fragments arranged by size:
3341 209 181
BsrGI T'GTAC_A
Cuts at: 436 579 436
Size: 143 3588
BssSI C'ACGA_G
Cuts at: 2002 2360 2002
Size: 358 3373
BstAPI GCAn_nnn'nTGC
Cuts at: 1108 1108
Size: 3731
BstBI TT'CG_AA
Cuts at: 2092 2092
Size: 3731
BstYI r'GATC_y
Cuts at: 1 137 756 1223 1246 1581 1827 2839 2925
Size: 136 619 467 23 335 246 1012 86
Cuts at: 2925 2937 3283 1
Size: 12 346 449
Fragments arranged by size:
1012 619 467 449 346 335 246 136
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3731
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1681 1862 2025 1681
Size: 181 163 3387
Fragments arranged by size:
3387 181 163
BtsI GCAGTG_nn'
Cuts at: 974 974
Size: 3731
ClaI AT'CG_AT
Cuts at: 479 479
Size: 3731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 242 277 242
Size: 35 3696
DraI TTT'AAA
Cuts at: 730 730
Size: 3731
DraIII CAC_nnn'GTG
Cuts at: 355 355
Size: 3731
DrdI GACnn_nn'nnGTC
Cuts at: 1437 2295 1437
Size: 858 2873
EaeI y'GGCC_r
Cuts at: 1316 1490 1881 1908 3253 1316
Size: 174 391 27 1345 1794
Fragments arranged by size:
1794 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1316 1316
Size: 3731
EarI CTCTTCn'nnn_
Cuts at: 378 1754 1964 378
Size: 1376 210 2145
Fragments arranged by size:
2145 1376 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1939 2249 2395 3541 1939
Size: 310 146 1146 2129
Fragments arranged by size:
2129 1146 310 146
EcoICRI GAG'CTC
Cuts at: 743 743
Size: 3731
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 1556 1988 2735 3003 154
Size: 1402 432 747 268 882
Fragments arranged by size:
1402 882 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 870 870
Size: 3731
EcoRI G'AATT_C
Cuts at: 735 735
Size: 3731
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1750 1750
Size: 3731
FspI TGC'GCA
Cuts at: 1512 1512
Size: 3731
HaeII r_GCGC'y
Cuts at: 1165 1413 2435 3414 1165
Size: 248 1022 979 1482
Fragments arranged by size:
1482 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1573 1603 1605 1635 1573
Size: 30 2 30 3669
Fragments arranged by size:
3669 30 30 2
HincII GTy'rAC
Cuts at: 237 770 237
Size: 533 3198
KasI G'GCGC_C
Cuts at: 1161 1409 1161
Size: 248 3483
KpnI G_GTAC'C
Cuts at: 751 751
Size: 3731
MluI A'CGCG_T
Cuts at: 3164 3164
Size: 3731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 447 2402 2586 447
Size: 1955 184 1592
Fragments arranged by size:
1955 1592 184
MscI TGG'CCA
Cuts at: 1492 1492
Size: 3731
MslI CAynn'nnrTG
Cuts at: 1720 1847 1720
Size: 127 3604
MspA1I CmG'CkG
Cuts at: 842 1156 1516 2529 2774 842
Size: 314 360 1013 245 1799
Fragments arranged by size:
1799 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1912 1912
Size: 3731
NarI GG'CG_CC
Cuts at: 1162 1410 1162
Size: 248 3483
NcoI C'CATG_G
Cuts at: 107 107
Size: 3731
NgoMIV G'CCGG_C
Cuts at: 1910 1910
Size: 3731
NspI r_CATG'y
Cuts at: 462 784 1284 2191 462
Size: 322 500 907 2002
Fragments arranged by size:
2002 907 500 322
PciI A'CATG_T
Cuts at: 2187 2187
Size: 3731
PmeI GTTT'AAAC
Cuts at: 730 730
Size: 3731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 312 344 2870 2902 312
Size: 32 2526 32 1141
Fragments arranged by size:
2526 1141 32 32
PstI C_TGCA'G
Cuts at: 778 1463 778
Size: 685 3046
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3731
PvuII CAG'CTG
Cuts at: 1156 1516 1156
Size: 360 3371
RsrII CG'GwC_CG
Cuts at: 1926 1926
Size: 3731
SacI G_AGCT'C
Cuts at: 745 745
Size: 3731
SalI G'TCGA_C
Cuts at: 768 768
Size: 3731
SapI GCTCTTCn'nnn_
Cuts at: 1754 1964 1754
Size: 210 3521
SbfI CC_TGCA'GG
Cuts at: 778 778
Size: 3731
ScaI AGT'ACT
Cuts at: 3248 3248
Size: 3731
SfcI C'TryA_G
Cuts at: 33 774 1459 2452 2643 33
Size: 741 685 993 191 1121
Fragments arranged by size:
1121 993 741 685 191
SfoI GGC'GCC
Cuts at: 1163 1411 1163
Size: 248 3483
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3731
SmaI CCC'GGG
Cuts at: 753 753
Size: 3731
SmlI C'TyrA_G
Cuts at: 183 886 1801 2293 2555 183
Size: 703 915 492 262 1359
Fragments arranged by size:
1359 915 703 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3731
SphI G_CATG'C
Cuts at: 784 1284 784
Size: 500 3231
StuI AGG'CCT
Cuts at: 515 515
Size: 3731
StyI C'CwwG_G
Cuts at: 107 687 865 1219 107
Size: 580 178 354 2619
Fragments arranged by size:
2619 580 354 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1314 1447 1906 2115 1314
Size: 133 459 209 2930
Fragments arranged by size:
2930 459 209 133
TatI w'GTAC_w
Cuts at: 436 579 3246 436
Size: 143 2667 921
Fragments arranged by size:
2667 921 143
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3248 3280 3248
Size: 32 3699
Tth111I GACn'n_nGTC
Cuts at: 1528 1528
Size: 3731
XbaI T'CTAG_A
Cuts at: 762 762
Size: 3731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1226 1226
Size: 3731
XmaI C'CCGG_G
Cuts at: 751 751
Size: 3731
XmnI GAAnn'nnTTC
Cuts at: 534 534
Size: 3731
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BbsI BbvCI BcgI BfrBI BglI BmtI
BpmI Bpu10I BsgI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI