Restriction map for
pF1KB8323
(Circular) (Six-base) MAPSORT of: pF1KB8323.seq Check: 4068 from: 1 to: 3872
pF1KB8323 3872 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 910 910
Size: 3872
Acc65I G'GTAC_C
Cuts at: 888 888
Size: 3872
AclI AA'CG_TT
Cuts at: 3712 3712
Size: 3872
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 1697 2129 2876 3144 157
Size: 1540 432 747 268 885
Fragments arranged by size:
1540 885 747 432 268
AflIII A'CryG_T
Cuts at: 631 1224 2328 3305 631
Size: 593 1104 977 1198
Fragments arranged by size:
1198 1104 977 593
AgeI A'CCGG_T
Cuts at: 2260 2260
Size: 3872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 697 697
Size: 3872
AlwNI CAG_nnn'CTG
Cuts at: 558 1177 2744 558
Size: 619 1567 1686
Fragments arranged by size:
1686 1567 619
ApaLI G'TGCA_C
Cuts at: 2642 2642
Size: 3872
ApoI r'AATT_y
Cuts at: 385 478 498 573 637 876 3225 3481 385
Size: 93 20 75 64 239 2349 256 776
Fragments arranged by size:
2349 776 256 239 93 75 64 20
AseI AT'TA_AT
Cuts at: 20 20
Size: 3872
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3872
AvaI C'yCGr_G
Cuts at: 319 892 319
Size: 573 3299
BamHI G'GATC_C
Cuts at: 897 897
Size: 3872
BanI G'GyrC_C
Cuts at: 888 1302 1550 1585 888
Size: 414 248 35 3175
Fragments arranged by size:
3175 414 248 35
BanII G_rGCy'C
Cuts at: 158 170 886 1916 158
Size: 12 716 1030 2114
Fragments arranged by size:
2114 1030 716 12
BbeI G_GCGC'C
Cuts at: 1306 1554 1306
Size: 248 3624
BciVI GTATCCnnnnn_n'
Cuts at: 1770 2531 3872 1770
Size: 761 1341 1770
Fragments arranged by size:
1770 1341 761
BclI T'GATC_A
Cuts at: 1392 1392
Size: 3872
BfrBI ATG'CAT
Cuts at: 778 778
Size: 3872
BglII A'GATC_T
Cuts at: 1387 3424 1387
Size: 2037 1835
BlpI GC'TnA_GC
Cuts at: 984 984
Size: 3872
Bme1580I G_kGCm'C
Cuts at: 1497 1590 2646 1497
Size: 93 1056 2723
Fragments arranged by size:
2723 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 806 1084 1165 1500 3644 806
Size: 278 81 335 2144 1034
Fragments arranged by size:
2144 1034 335 278 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2239 2239
Size: 3872
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 780 547
Size: 233 3639
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1048 1927 2419 2717 1048
Size: 879 492 298 2203
Fragments arranged by size:
2203 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 541 3576 541
Size: 3035 837
BsaAI yAC'GTr
Cuts at: 634 1225 634
Size: 591 3281
BsaBI GATnn'nnATC
Cuts at: 6 566 1386 3423 6
Size: 560 820 2037 455
Fragments arranged by size:
2037 820 560 455
BsaHI Gr'CG_yC
Cuts at: 1303 1551 2253 3749 1303
Size: 248 702 1496 1426
Fragments arranged by size:
1496 1426 702 248
BsaWI w'CCGG_w
Cuts at: 1061 1582 2260 2534 2681 3092 1061
Size: 521 678 274 147 411 1841
Fragments arranged by size:
1841 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 569 157
Size: 412 3460
BseYI C'CCAG_C
Cuts at: 1297 2055 2632 3128 1297
Size: 758 577 496 2041
Fragments arranged by size:
2041 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1414 1460 2668 104
Size: 1310 46 1208 1308
Fragments arranged by size:
1310 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 170 886 1664 1854 2646 170
Size: 716 778 190 792 1396
Fragments arranged by size:
1396 792 778 716 190
Bsp1286I G_dGCh'C
Cuts at: 158 170 886 1497 1590 1664 1854 1916 2646
Size: 12 716 611 93 74 190 62 730
Cuts at: 2646 158
Size: 1384
Fragments arranged by size:
1384 730 716 611 190 93 74 62
12
BspEI T'CCGG_A
Cuts at: 3092 3092
Size: 3872
BspHI T'CATG_A
Cuts at: 233 3048 233
Size: 2815 1057
BspMI ACCTGCnnnn'nnnn_
Cuts at: 311 686 922 1136 1438 1819 311
Size: 375 236 214 302 381 2364
Fragments arranged by size:
2364 381 375 302 236 214
BsrBI CCG'CTC
Cuts at: 50 2164 2218 3702 3867 50
Size: 2114 54 1484 165 55
Fragments arranged by size:
2114 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 657 1784 657
Size: 1127 2745
BsrFI r'CCGG_y
Cuts at: 1870 2051 2260 1870
Size: 181 209 3482
Fragments arranged by size:
3482 209 181
BssSI C'ACGA_G
Cuts at: 2143 2501 2143
Size: 358 3514
BstAPI GCAn_nnn'nTGC
Cuts at: 1249 1249
Size: 3872
BstBI TT'CG_AA
Cuts at: 2233 2233
Size: 3872
BstYI r'GATC_y
Cuts at: 1 897 1364 1387 1722 1968 2980 3066 3078
Size: 896 467 23 335 246 1012 86 12
Cuts at: 3078 3424 1
Size: 346 449
Fragments arranged by size:
1012 896 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3872
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1822 2003 2166 1822
Size: 181 163 3528
Fragments arranged by size:
3528 181 163
BtsI GCAGTG_nn'
Cuts at: 192 1115 192
Size: 923 2949
DraI TTT'AAA
Cuts at: 871 871
Size: 3872
DrdI GACnn_nn'nnGTC
Cuts at: 1578 2436 1578
Size: 858 3014
EaeI y'GGCC_r
Cuts at: 710 1457 1631 2022 2049 3394 710
Size: 747 174 391 27 1345 1188
Fragments arranged by size:
1345 1188 747 391 174 27
EagI C'GGCC_G
Cuts at: 1457 1457
Size: 3872
EarI CTCTTCn'nnn_
Cuts at: 175 235 389 1895 2105 175
Size: 60 154 1506 210 1942
Fragments arranged by size:
1942 1506 210 154 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2080 2390 2536 3682 2080
Size: 310 146 1146 2270
Fragments arranged by size:
2270 1146 310 146
EcoICRI GAG'CTC
Cuts at: 168 884 168
Size: 716 3156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 547 780 1697 2129 2876 3144 157
Size: 390 233 917 432 747 268 885
Fragments arranged by size:
917 885 747 432 390 268 233
EcoO109I rG'GnC_Cy
Cuts at: 323 1011 323
Size: 688 3184
EcoRI G'AATT_C
Cuts at: 498 573 876 498
Size: 75 303 3494
Fragments arranged by size:
3494 303 75
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1891 1891
Size: 3872
FspI TGC'GCA
Cuts at: 1653 1653
Size: 3872
HaeII r_GCGC'y
Cuts at: 1306 1554 2576 3555 1306
Size: 248 1022 979 1623
Fragments arranged by size:
1623 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1714 1744 1746 1776 1714
Size: 30 2 30 3810
Fragments arranged by size:
3810 30 30 2
HincII GTy'rAC
Cuts at: 279 911 279
Size: 632 3240
HindIII A'AGCT_T
Cuts at: 424 424
Size: 3872
KasI G'GCGC_C
Cuts at: 1302 1550 1302
Size: 248 3624
KpnI G_GTAC'C
Cuts at: 892 892
Size: 3872
MluI A'CGCG_T
Cuts at: 3305 3305
Size: 3872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2543 2727 2543
Size: 184 3688
MscI TGG'CCA
Cuts at: 712 1633 712
Size: 921 2951
MslI CAynn'nnrTG
Cuts at: 1861 1988 1861
Size: 127 3745
MspA1I CmG'CkG
Cuts at: 126 983 1297 1657 2670 2915 126
Size: 857 314 360 1013 245 1083
Fragments arranged by size:
1083 1013 857 360 314 245
NaeI GCC'GGC
Cuts at: 2053 2053
Size: 3872
NarI GG'CG_CC
Cuts at: 1303 1551 1303
Size: 248 3624
NcoI C'CATG_G
Cuts at: 107 107
Size: 3872
NgoMIV G'CCGG_C
Cuts at: 2051 2051
Size: 3872
NsiI A_TGCA'T
Cuts at: 780 780
Size: 3872
NspI r_CATG'y
Cuts at: 925 1425 2332 925
Size: 500 907 2465
Fragments arranged by size:
2465 907 500
PciI A'CATG_T
Cuts at: 2328 2328
Size: 3872
PmeI GTTT'AAAC
Cuts at: 871 871
Size: 3872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 3840
PpuMI rG'GwC_Cy
Cuts at: 323 323
Size: 3872
PsiI TTA'TAA
Cuts at: 340 340
Size: 3872
PstI C_TGCA'G
Cuts at: 308 554 919 1604 308
Size: 246 365 685 2576
Fragments arranged by size:
2576 685 365 246
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3872
PvuII CAG'CTG
Cuts at: 1297 1657 1297
Size: 360 3512
RsrII CG'GwC_CG
Cuts at: 2067 2067
Size: 3872
SacI G_AGCT'C
Cuts at: 170 886 170
Size: 716 3156
SalI G'TCGA_C
Cuts at: 909 909
Size: 3872
SanDI GG'GwC_CC
Cuts at: 323 323
Size: 3872
SapI GCTCTTCn'nnn_
Cuts at: 175 235 1895 2105 175
Size: 60 1660 210 1942
Fragments arranged by size:
1942 1660 210 60
SbfI CC_TGCA'GG
Cuts at: 919 919
Size: 3872
ScaI AGT'ACT
Cuts at: 418 3389 418
Size: 2971 901
SfcI C'TryA_G
Cuts at: 33 304 550 915 1600 2593 2784 33
Size: 271 246 365 685 993 191 1121
Fragments arranged by size:
1121 993 685 365 271 246 191
SfoI GGC'GCC
Cuts at: 1304 1552 1304
Size: 248 3624
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3872
SmaI CCC'GGG
Cuts at: 321 894 321
Size: 573 3299
SmlI C'TyrA_G
Cuts at: 1027 1942 2434 2696 1027
Size: 915 492 262 2203
Fragments arranged by size:
2203 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3872
SphI G_CATG'C
Cuts at: 925 1425 925
Size: 500 3372
StyI C'CwwG_G
Cuts at: 107 863 1006 1360 107
Size: 756 143 354 2619
Fragments arranged by size:
2619 756 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 344 455 1455 1588 2047 2256 344
Size: 111 1000 133 459 209 1960
Fragments arranged by size:
1960 1000 459 209 133 111
TatI w'GTAC_w
Cuts at: 416 3387 416
Size: 2971 901
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3389 3421 3389
Size: 32 3840
Tth111I GACn'n_nGTC
Cuts at: 1669 1669
Size: 3872
XbaI T'CTAG_A
Cuts at: 903 903
Size: 3872
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1367 1367
Size: 3872
XmaI C'CCGG_G
Cuts at: 319 892 319
Size: 573 3299
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI PacI PasI PflMI PfoI PmlI PshAI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI