Restriction map for
pF1KB8321
(Circular) (Six-base) MAPSORT of: pF1KB8321.seq Check: 5342 from: 1 to: 3989
pF1KB8321 3989 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1027 1027
Size: 3989
Acc65I G'GTAC_C
Cuts at: 1005 1005
Size: 3989
AclI AA'CG_TT
Cuts at: 3829 3829
Size: 3989
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 1814 2246 2993 3261 718
Size: 1096 432 747 268 1446
Fragments arranged by size:
1446 1096 747 432 268
AflII C'TTAA_G
Cuts at: 721 721
Size: 3989
AflIII A'CryG_T
Cuts at: 425 1341 2445 3422 425
Size: 916 1104 977 992
Fragments arranged by size:
1104 992 977 916
AgeI A'CCGG_T
Cuts at: 2377 2377
Size: 3989
AleI CACnn'nnGTG
Cuts at: 901 901
Size: 3989
AlwNI CAG_nnn'CTG
Cuts at: 300 1294 2861 300
Size: 994 1567 1428
Fragments arranged by size:
1567 1428 994
ApaLI G'TGCA_C
Cuts at: 2759 2759
Size: 3989
ApoI r'AATT_y
Cuts at: 232 265 406 646 993 3342 3598 232
Size: 33 141 240 347 2349 256 623
Fragments arranged by size:
2349 623 347 256 240 141 33
AseI AT'TA_AT
Cuts at: 20 20
Size: 3989
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3989
AvaI C'yCGr_G
Cuts at: 1009 1009
Size: 3989
BamHI G'GATC_C
Cuts at: 1014 1014
Size: 3989
BanI G'GyrC_C
Cuts at: 1005 1419 1667 1702 1005
Size: 414 248 35 3292
Fragments arranged by size:
3292 414 248 35
BanII G_rGCy'C
Cuts at: 1003 2033 1003
Size: 1030 2959
BbeI G_GCGC'C
Cuts at: 1423 1671 1423
Size: 248 3741
BbsI GAAGACnn'nnnn_
Cuts at: 724 724
Size: 3989
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3955
BciVI GTATCCnnnnn_n'
Cuts at: 380 1887 2648 3989 380
Size: 1507 761 1341 380
Fragments arranged by size:
1507 1341 761 380
BclI T'GATC_A
Cuts at: 747 1509 747
Size: 762 3227
BglII A'GATC_T
Cuts at: 447 1504 3541 447
Size: 1057 2037 895
Fragments arranged by size:
2037 1057 895
BlpI GC'TnA_GC
Cuts at: 1101 1101
Size: 3989
Bme1580I G_kGCm'C
Cuts at: 1614 1707 2763 1614
Size: 93 1056 2840
Fragments arranged by size:
2840 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1201 1282 1617 3761 1201
Size: 81 335 2144 1429
Fragments arranged by size:
2144 1429 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 562 2356 562
Size: 1794 2195
Bpu10I CC'TnA_GC
Cuts at: 891 891
Size: 3989
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1165 2044 2536 2834 1165
Size: 879 492 298 2320
Fragments arranged by size:
2320 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3693 3693
Size: 3989
BsaAI yAC'GTr
Cuts at: 1342 1342
Size: 3989
BsaBI GATnn'nnATC
Cuts at: 6 952 1503 3540 6
Size: 946 551 2037 455
Fragments arranged by size:
2037 946 551 455
BsaHI Gr'CG_yC
Cuts at: 1420 1668 2370 3866 1420
Size: 248 702 1496 1543
Fragments arranged by size:
1543 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1178 1699 2377 2651 2798 3209 1178
Size: 521 678 274 147 411 1958
Fragments arranged by size:
1958 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 415 445 415
Size: 30 3959
BseYI C'CCAG_C
Cuts at: 336 1414 2172 2749 3245 336
Size: 1078 758 577 496 1080
Fragments arranged by size:
1080 1078 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 493
Size: 3989
BsiEI CG_ry'CG
Cuts at: 104 1531 1577 2785 104
Size: 1427 46 1208 1308
Fragments arranged by size:
1427 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 203 1003 1781 1971 2763 203
Size: 800 778 190 792 1429
Fragments arranged by size:
1429 800 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 203 1003 1614 1707 1781 1971 2033 2763 203
Size: 800 611 93 74 190 62 730 1429
Fragments arranged by size:
1429 800 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3209 3209
Size: 3989
BspHI T'CATG_A
Cuts at: 3165 3165
Size: 3989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 972 1039 1253 1555 1936 972
Size: 67 214 302 381 3025
Fragments arranged by size:
3025 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 2281 2335 3819 3984 50
Size: 2231 54 1484 165 55
Fragments arranged by size:
2231 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 315 1901 315
Size: 1586 2403
BsrFI r'CCGG_y
Cuts at: 1987 2168 2377 1987
Size: 181 209 3599
Fragments arranged by size:
3599 209 181
BssSI C'ACGA_G
Cuts at: 2260 2618 2260
Size: 358 3631
BstAPI GCAn_nnn'nTGC
Cuts at: 1366 1366
Size: 3989
BstBI TT'CG_AA
Cuts at: 2350 2350
Size: 3989
BstYI r'GATC_y
Cuts at: 1 185 447 1014 1481 1504 1839 2085 3097
Size: 184 262 567 467 23 335 246 1012
Cuts at: 3097 3183 3195 3541 1
Size: 86 12 346 449
Fragments arranged by size:
1012 567 467 449 346 335 262 246
184 86 23 12
BtgI C'CryG_G
Cuts at: 221 221
Size: 3989
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1939 2120 2283 1939
Size: 181 163 3645
Fragments arranged by size:
3645 181 163
BtsI GCAGTG_nn'
Cuts at: 505 799 1232 505
Size: 294 433 3262
Fragments arranged by size:
3262 433 294
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 855 890 855
Size: 35 3954
DraI TTT'AAA
Cuts at: 323 988 323
Size: 665 3324
DrdI GACnn_nn'nnGTC
Cuts at: 1695 2553 1695
Size: 858 3131
EaeI y'GGCC_r
Cuts at: 1574 1748 2139 2166 3511 1574
Size: 174 391 27 1345 2052
Fragments arranged by size:
2052 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1574 1574
Size: 3989
EarI CTCTTCn'nnn_
Cuts at: 167 482 629 700 2012 2222 167
Size: 315 147 71 1312 210 1934
Fragments arranged by size:
1934 1312 315 210 147 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2197 2507 2653 3799 2197
Size: 310 146 1146 2387
Fragments arranged by size:
2387 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1001 1001
Size: 3989
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 1814 2246 2993 3261 718
Size: 1096 432 747 268 1446
Fragments arranged by size:
1446 1096 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 418 418
Size: 3989
EcoO109I rG'GnC_Cy
Cuts at: 1128 1128
Size: 3989
EcoRI G'AATT_C
Cuts at: 646 993 646
Size: 347 3642
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2008 2008
Size: 3989
FspI TGC'GCA
Cuts at: 1770 1770
Size: 3989
HaeII r_GCGC'y
Cuts at: 1423 1671 2693 3672 1423
Size: 248 1022 979 1740
Fragments arranged by size:
1740 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 415 447 916 948 1831 1861 1863 1893 415
Size: 32 469 32 883 30 2 30 2511
Fragments arranged by size:
2511 883 469 32 32 30 30 2
HincII GTy'rAC
Cuts at: 315 1028 315
Size: 713 3276
KasI G'GCGC_C
Cuts at: 1419 1667 1419
Size: 248 3741
KpnI G_GTAC'C
Cuts at: 1009 1009
Size: 3989
MfeI C'AATT_G
Cuts at: 257 257
Size: 3989
MluI A'CGCG_T
Cuts at: 3422 3422
Size: 3989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 734 2660 2844 734
Size: 1926 184 1879
Fragments arranged by size:
1926 1879 184
MscI TGG'CCA
Cuts at: 1750 1750
Size: 3989
MslI CAynn'nnrTG
Cuts at: 901 1978 2105 901
Size: 1077 127 2785
Fragments arranged by size:
2785 1077 127
MspA1I CmG'CkG
Cuts at: 336 1100 1414 1774 2787 3032 336
Size: 764 314 360 1013 245 1293
Fragments arranged by size:
1293 1013 764 360 314 245
NaeI GCC'GGC
Cuts at: 2170 2170
Size: 3989
NarI GG'CG_CC
Cuts at: 1420 1668 1420
Size: 248 3741
NcoI C'CATG_G
Cuts at: 221 221
Size: 3989
NgoMIV G'CCGG_C
Cuts at: 2168 2168
Size: 3989
NspI r_CATG'y
Cuts at: 429 978 1042 1542 2449 429
Size: 549 64 500 907 1969
Fragments arranged by size:
1969 907 549 500 64
PciI A'CATG_T
Cuts at: 425 2445 425
Size: 2020 1969
PmeI GTTT'AAAC
Cuts at: 988 988
Size: 3989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3128 3160 3128
Size: 32 3957
PstI C_TGCA'G
Cuts at: 117 1036 1721 117
Size: 919 685 2385
Fragments arranged by size:
2385 919 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3989
PvuII CAG'CTG
Cuts at: 336 1414 1774 336
Size: 1078 360 2551
Fragments arranged by size:
2551 1078 360
RsrII CG'GwC_CG
Cuts at: 2184 2184
Size: 3989
SacI G_AGCT'C
Cuts at: 1003 1003
Size: 3989
SalI G'TCGA_C
Cuts at: 1026 1026
Size: 3989
SapI GCTCTTCn'nnn_
Cuts at: 700 2012 2222 700
Size: 1312 210 2467
Fragments arranged by size:
2467 1312 210
SbfI CC_TGCA'GG
Cuts at: 1036 1036
Size: 3989
ScaI AGT'ACT
Cuts at: 871 3506 871
Size: 2635 1354
SfcI C'TryA_G
Cuts at: 33 113 469 1032 1717 2710 2901 33
Size: 80 356 563 685 993 191 1121
Fragments arranged by size:
1121 993 685 563 356 191 80
SfoI GGC'GCC
Cuts at: 1421 1669 1421
Size: 248 3741
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3989
SmaI CCC'GGG
Cuts at: 1011 1011
Size: 3989
SmlI C'TyrA_G
Cuts at: 721 1144 2059 2551 2813 721
Size: 423 915 492 262 1897
Fragments arranged by size:
1897 915 492 423 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3989
SphI G_CATG'C
Cuts at: 1042 1542 1042
Size: 500 3489
StyI C'CwwG_G
Cuts at: 221 1123 1477 221
Size: 902 354 2733
Fragments arranged by size:
2733 902 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1572 1705 2164 2373 1572
Size: 133 459 209 3188
Fragments arranged by size:
3188 459 209 133
TatI w'GTAC_w
Cuts at: 428 869 3504 428
Size: 441 2635 913
Fragments arranged by size:
2635 913 441
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 219 251 3506 3538 219
Size: 32 3255 32 670
Fragments arranged by size:
3255 670 32 32
Tth111I GACn'n_nGTC
Cuts at: 556 1786 556
Size: 1230 2759
XbaI T'CTAG_A
Cuts at: 165 1020 165
Size: 855 3134
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1484 1484
Size: 3989
XmaI C'CCGG_G
Cuts at: 1009 1009
Size: 3989
XmnI GAAnn'nnTTC
Cuts at: 650 667 650
Size: 17 3972
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI BpmI
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI