Restriction map for
pF1KB8319
(Circular) (Six-base) MAPSORT of: pF1KB8319.seq Check: 1002 from: 1 to: 4010
pF1KB8319 4010 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1048 1048
Size: 4010
Acc65I G'GTAC_C
Cuts at: 1026 1026
Size: 4010
AclI AA'CG_TT
Cuts at: 3850 3850
Size: 4010
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 605 1835 2267 3014 3282 373
Size: 232 1230 432 747 268 1101
Fragments arranged by size:
1230 1101 747 432 268 232
AflII C'TTAA_G
Cuts at: 589 589
Size: 4010
AflIII A'CryG_T
Cuts at: 1362 2466 3443 1362
Size: 1104 977 1929
Fragments arranged by size:
1929 1104 977
AgeI A'CCGG_T
Cuts at: 2398 2398
Size: 4010
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 304 304
Size: 4010
AlwNI CAG_nnn'CTG
Cuts at: 1315 2882 1315
Size: 1567 2443
ApaLI G'TGCA_C
Cuts at: 2780 2780
Size: 4010
ApoI r'AATT_y
Cuts at: 1014 3363 3619 1014
Size: 2349 256 1405
Fragments arranged by size:
2349 1405 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4010
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
AvaI C'yCGr_G
Cuts at: 1030 1030
Size: 4010
BamHI G'GATC_C
Cuts at: 323 726 1035 323
Size: 403 309 3298
Fragments arranged by size:
3298 403 309
BanI G'GyrC_C
Cuts at: 1026 1440 1688 1723 1026
Size: 414 248 35 3313
Fragments arranged by size:
3313 414 248 35
BanII G_rGCy'C
Cuts at: 470 871 1024 2054 470
Size: 401 153 1030 2426
Fragments arranged by size:
2426 1030 401 153
BbeI G_GCGC'C
Cuts at: 1444 1692 1444
Size: 248 3762
BbsI GAAGACnn'nnnn_
Cuts at: 403 605 403
Size: 202 3808
BciVI GTATCCnnnnn_n'
Cuts at: 1908 2669 4010 1908
Size: 761 1341 1908
Fragments arranged by size:
1908 1341 761
BclI T'GATC_A
Cuts at: 1530 1530
Size: 4010
BglII A'GATC_T
Cuts at: 1525 3562 1525
Size: 2037 1973
BlpI GC'TnA_GC
Cuts at: 1122 1122
Size: 4010
Bme1580I G_kGCm'C
Cuts at: 1635 1728 2784 1635
Size: 93 1056 2861
Fragments arranged by size:
2861 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 163 1222 1303 1638 3782 163
Size: 1059 81 335 2144 391
Fragments arranged by size:
2144 1059 391 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1186 2065 2557 2855 1186
Size: 879 492 298 2341
Fragments arranged by size:
2341 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3714 3714
Size: 4010
BsaAI yAC'GTr
Cuts at: 1363 1363
Size: 4010
BsaBI GATnn'nnATC
Cuts at: 6 989 1524 3561 6
Size: 983 535 2037 455
Fragments arranged by size:
2037 983 535 455
BsaHI Gr'CG_yC
Cuts at: 1441 1689 2391 3887 1441
Size: 248 702 1496 1564
Fragments arranged by size:
1564 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1199 1720 2398 2672 2819 3230 1199
Size: 521 678 274 147 411 1979
Fragments arranged by size:
1979 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1435 2193 2770 3266 1435
Size: 758 577 496 2179
Fragments arranged by size:
2179 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 798 915 274
Size: 524 117 3369
Fragments arranged by size:
3369 524 117
BsiEI CG_ry'CG
Cuts at: 104 1552 1598 2806 104
Size: 1448 46 1208 1308
Fragments arranged by size:
1448 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 871 1024 1802 1992 2784 871
Size: 153 778 190 792 2097
Fragments arranged by size:
2097 792 778 190 153
Bsp1286I G_dGCh'C
Cuts at: 470 871 1024 1635 1728 1802 1992 2054 2784
Size: 401 153 611 93 74 190 62 730
Cuts at: 2784 470
Size: 1696
Fragments arranged by size:
1696 730 611 401 190 153 93 74
62
BspEI T'CCGG_A
Cuts at: 3230 3230
Size: 4010
BspHI T'CATG_A
Cuts at: 513 3186 513
Size: 2673 1337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 536 639 1060 1274 1576 1957 536
Size: 103 421 214 302 381 2589
Fragments arranged by size:
2589 421 381 302 214 103
BsrBI CCG'CTC
Cuts at: 50 2302 2356 3840 4005 50
Size: 2252 54 1484 165 55
Fragments arranged by size:
2252 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 225 268 1922 225
Size: 43 1654 2313
Fragments arranged by size:
2313 1654 43
BsrFI r'CCGG_y
Cuts at: 214 2008 2189 2398 214
Size: 1794 181 209 1826
Fragments arranged by size:
1826 1794 209 181
BssSI C'ACGA_G
Cuts at: 2281 2639 2281
Size: 358 3652
BstAPI GCAn_nnn'nTGC
Cuts at: 258 1387 258
Size: 1129 2881
BstBI TT'CG_AA
Cuts at: 2371 2371
Size: 4010
BstXI CCAn_nnnn'nTGG
Cuts at: 681 681
Size: 4010
BstYI r'GATC_y
Cuts at: 1 122 323 699 726 750 1035 1502 1525
Size: 121 201 376 27 24 285 467 23
Cuts at: 1525 1860 2106 3118 3204 3216 3562 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 376 346 335 285 246
201 121 86 27 24 23 12
Bsu36I CC'TnA_GG
Cuts at: 479 479
Size: 4010
BtgI C'CryG_G
Cuts at: 107 107
Size: 4010
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 923 1960 2141 2304 923
Size: 1037 181 163 2629
Fragments arranged by size:
2629 1037 181 163
BtsI GCAGTG_nn'
Cuts at: 152 1253 152
Size: 1101 2909
DraI TTT'AAA
Cuts at: 1009 1009
Size: 4010
DrdI GACnn_nn'nnGTC
Cuts at: 1716 2574 1716
Size: 858 3152
EaeI y'GGCC_r
Cuts at: 132 1595 1769 2160 2187 3532 132
Size: 1463 174 391 27 1345 610
Fragments arranged by size:
1463 1345 610 391 174 27
EagI C'GGCC_G
Cuts at: 1595 1595
Size: 4010
EarI CTCTTCn'nnn_
Cuts at: 2033 2243 2033
Size: 210 3800
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2218 2528 2674 3820 2218
Size: 310 146 1146 2408
Fragments arranged by size:
2408 1146 310 146
EcoICRI GAG'CTC
Cuts at: 869 1022 869
Size: 153 3857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 605 1835 2267 3014 3282 373
Size: 232 1230 432 747 268 1101
Fragments arranged by size:
1230 1101 747 432 268 232
EcoO109I rG'GnC_Cy
Cuts at: 1149 1149
Size: 4010
EcoRI G'AATT_C
Cuts at: 1014 1014
Size: 4010
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2029 2029
Size: 4010
FspI TGC'GCA
Cuts at: 1791 1791
Size: 4010
HaeII r_GCGC'y
Cuts at: 1444 1692 2714 3693 1444
Size: 248 1022 979 1761
Fragments arranged by size:
1761 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 148 180 1852 1882 1884 1914 148
Size: 32 1672 30 2 30 2244
Fragments arranged by size:
2244 1672 32 30 30 2
HincII GTy'rAC
Cuts at: 212 1049 212
Size: 837 3173
HindIII A'AGCT_T
Cuts at: 199 592 199
Size: 393 3617
HpaI GTT'AAC
Cuts at: 212 212
Size: 4010
KasI G'GCGC_C
Cuts at: 1440 1688 1440
Size: 248 3762
KpnI G_GTAC'C
Cuts at: 1030 1030
Size: 4010
MluI A'CGCG_T
Cuts at: 3443 3443
Size: 4010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2681 2865 2681
Size: 184 3826
MscI TGG'CCA
Cuts at: 134 1771 134
Size: 1637 2373
MslI CAynn'nnrTG
Cuts at: 518 1999 2126 518
Size: 1481 127 2402
Fragments arranged by size:
2402 1481 127
MspA1I CmG'CkG
Cuts at: 255 373 462 920 929 1121 1435 1795 2808
Size: 118 89 458 9 192 314 360 1013
Cuts at: 2808 3053 255
Size: 245 1212
Fragments arranged by size:
1212 1013 458 360 314 245 192 118
89 9
NaeI GCC'GGC
Cuts at: 2191 2191
Size: 4010
NarI GG'CG_CC
Cuts at: 1441 1689 1441
Size: 248 3762
NcoI C'CATG_G
Cuts at: 107 107
Size: 4010
NgoMIV G'CCGG_C
Cuts at: 2189 2189
Size: 4010
NspI r_CATG'y
Cuts at: 1063 1563 2470 1063
Size: 500 907 2603
Fragments arranged by size:
2603 907 500
PciI A'CATG_T
Cuts at: 2466 2466
Size: 4010
PflMI CCAn_nnn'nTGG
Cuts at: 141 659 141
Size: 518 3492
PmeI GTTT'AAAC
Cuts at: 1009 1009
Size: 4010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 3978
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 479 511 479
Size: 32 3978
PstI C_TGCA'G
Cuts at: 461 1057 1742 461
Size: 596 685 2729
Fragments arranged by size:
2729 685 596
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4010
PvuII CAG'CTG
Cuts at: 255 373 929 1435 1795 255
Size: 118 556 506 360 2470
Fragments arranged by size:
2470 556 506 360 118
RsrII CG'GwC_CG
Cuts at: 2205 2205
Size: 4010
SacI G_AGCT'C
Cuts at: 871 1024 871
Size: 153 3857
SalI G'TCGA_C
Cuts at: 1047 1047
Size: 4010
SapI GCTCTTCn'nnn_
Cuts at: 2033 2243 2033
Size: 210 3800
SbfI CC_TGCA'GG
Cuts at: 1057 1057
Size: 4010
ScaI AGT'ACT
Cuts at: 3527 3527
Size: 4010
SfcI C'TryA_G
Cuts at: 33 374 457 1053 1738 2731 2922 33
Size: 341 83 596 685 993 191 1121
Fragments arranged by size:
1121 993 685 596 341 191 83
SfoI GGC'GCC
Cuts at: 1442 1690 1442
Size: 248 3762
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
SmaI CCC'GGG
Cuts at: 1032 1032
Size: 4010
SmlI C'TyrA_G
Cuts at: 589 1165 2080 2572 2834 589
Size: 576 915 492 262 1765
Fragments arranged by size:
1765 915 576 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4010
SphI G_CATG'C
Cuts at: 1063 1563 1063
Size: 500 3510
StuI AGG'CCT
Cuts at: 802 802
Size: 4010
StyI C'CwwG_G
Cuts at: 107 507 939 1144 1498 107
Size: 400 432 205 354 2619
Fragments arranged by size:
2619 432 400 354 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1593 1726 2185 2394 1593
Size: 133 459 209 3209
Fragments arranged by size:
3209 459 209 133
TatI w'GTAC_w
Cuts at: 486 3525 486
Size: 3039 971
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 3978
Tth111I GACn'n_nGTC
Cuts at: 1807 1807
Size: 4010
XbaI T'CTAG_A
Cuts at: 1041 1041
Size: 4010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1505 1505
Size: 4010
XmaI C'CCGG_G
Cuts at: 1030 1030
Size: 4010
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI