Restriction map for
pF1KB8318
(Circular) (Six-base) MAPSORT of: pF1KB8318.seq Check: 2665 from: 1 to: 3854
pF1KB8318 3854 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 892 892
Size: 3854
Acc65I G'GTAC_C
Cuts at: 307 401 870 307
Size: 94 469 3291
Fragments arranged by size:
3291 469 94
AclI AA'CG_TT
Cuts at: 3694 3694
Size: 3854
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 818 1679 2111 2858 3126 302
Size: 516 861 432 747 268 1030
Fragments arranged by size:
1030 861 747 516 432 268
AflIII A'CryG_T
Cuts at: 788 790 1206 2310 3287 788
Size: 2 416 1104 977 1355
Fragments arranged by size:
1355 1104 977 416 2
AgeI A'CCGG_T
Cuts at: 2242 2242
Size: 3854
AleI CACnn'nnGTG
Cuts at: 133 133
Size: 3854
AlwNI CAG_nnn'CTG
Cuts at: 1159 2726 1159
Size: 1567 2287
ApaLI G'TGCA_C
Cuts at: 2624 2624
Size: 3854
ApoI r'AATT_y
Cuts at: 244 507 532 730 778 858 3207 3463 244
Size: 263 25 198 48 80 2349 256 635
Fragments arranged by size:
2349 635 263 256 198 80 48 25
AseI AT'TA_AT
Cuts at: 20 20
Size: 3854
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3854
AvaI C'yCGr_G
Cuts at: 874 874
Size: 3854
BamHI G'GATC_C
Cuts at: 879 879
Size: 3854
BanI G'GyrC_C
Cuts at: 307 401 870 1284 1532 1567 307
Size: 94 469 414 248 35 2594
Fragments arranged by size:
2594 469 414 248 94 35
BanII G_rGCy'C
Cuts at: 868 1898 868
Size: 1030 2824
BbeI G_GCGC'C
Cuts at: 1288 1536 1288
Size: 248 3606
BbsI GAAGACnn'nnnn_
Cuts at: 624 806 624
Size: 182 3672
BbvCI CC'TCA_GC
Cuts at: 596 596
Size: 3854
BciVI GTATCCnnnnn_n'
Cuts at: 1752 2513 3854 1752
Size: 761 1341 1752
Fragments arranged by size:
1752 1341 761
BclI T'GATC_A
Cuts at: 1374 1374
Size: 3854
BglII A'GATC_T
Cuts at: 1369 3406 1369
Size: 2037 1817
BlpI GC'TnA_GC
Cuts at: 966 966
Size: 3854
Bme1580I G_kGCm'C
Cuts at: 1479 1572 2628 1479
Size: 93 1056 2705
Fragments arranged by size:
2705 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 601 1066 1147 1482 3626 601
Size: 465 81 335 2144 829
Fragments arranged by size:
2144 829 465 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2221 2221
Size: 3854
Bpu10I CC'TnA_GC
Cuts at: 596 596
Size: 3854
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 116 1030 1909 2401 2699 116
Size: 914 879 492 298 1271
Fragments arranged by size:
1271 914 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3558 3558
Size: 3854
BsaAI yAC'GTr
Cuts at: 791 1207 791
Size: 416 3438
BsaBI GATnn'nnATC
Cuts at: 6 1368 3405 6
Size: 1362 2037 455
Fragments arranged by size:
2037 1362 455
BsaHI Gr'CG_yC
Cuts at: 1285 1533 2235 3731 1285
Size: 248 702 1496 1408
Fragments arranged by size:
1496 1408 702 248
BsaWI w'CCGG_w
Cuts at: 1043 1564 2242 2516 2663 3074 1043
Size: 521 678 274 147 411 1823
Fragments arranged by size:
1823 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 300 330 375 405 300
Size: 30 45 30 3749
Fragments arranged by size:
3749 45 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 382 383 382
Size: 1 3853
BseYI C'CCAG_C
Cuts at: 1279 2037 2614 3110 1279
Size: 758 577 496 2023
Fragments arranged by size:
2023 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1396 1442 2650 104
Size: 1292 46 1208 1308
Fragments arranged by size:
1308 1292 1208 46
BsiHKAI G_wGCw'C
Cuts at: 868 1646 1836 2628 868
Size: 778 190 792 2094
Fragments arranged by size:
2094 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 868 1479 1572 1646 1836 1898 2628 868
Size: 611 93 74 190 62 730 2094
Fragments arranged by size:
2094 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3074 3074
Size: 3854
BspHI T'CATG_A
Cuts at: 218 3030 218
Size: 2812 1042
BspMI ACCTGCnnnn'nnnn_
Cuts at: 904 1118 1420 1801 904
Size: 214 302 381 2957
Fragments arranged by size:
2957 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2146 2200 3684 3849 50
Size: 2096 54 1484 165 55
Fragments arranged by size:
2096 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1766 1766
Size: 3854
BsrFI r'CCGG_y
Cuts at: 1852 2033 2242 1852
Size: 181 209 3464
Fragments arranged by size:
3464 209 181
BssSI C'ACGA_G
Cuts at: 2125 2483 2125
Size: 358 3496
BstAPI GCAn_nnn'nTGC
Cuts at: 1231 1231
Size: 3854
BstBI TT'CG_AA
Cuts at: 2215 2215
Size: 3854
BstYI r'GATC_y
Cuts at: 1 879 1346 1369 1704 1950 2962 3048 3060
Size: 878 467 23 335 246 1012 86 12
Cuts at: 3060 3406 1
Size: 346 449
Fragments arranged by size:
1012 878 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1804 1985 2148 1804
Size: 181 163 3510
Fragments arranged by size:
3510 181 163
BtsI GCAGTG_nn'
Cuts at: 1097 1097
Size: 3854
DraI TTT'AAA
Cuts at: 853 853
Size: 3854
DrdI GACnn_nn'nnGTC
Cuts at: 1560 2418 1560
Size: 858 2996
EaeI y'GGCC_r
Cuts at: 1439 1613 2004 2031 3376 1439
Size: 174 391 27 1345 1917
Fragments arranged by size:
1917 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1439 1439
Size: 3854
EarI CTCTTCn'nnn_
Cuts at: 838 1877 2087 838
Size: 1039 210 2605
Fragments arranged by size:
2605 1039 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2062 2372 2518 3664 2062
Size: 310 146 1146 2252
Fragments arranged by size:
2252 1146 310 146
EcoICRI GAG'CTC
Cuts at: 866 866
Size: 3854
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 818 1679 2111 2858 3126 302
Size: 516 861 432 747 268 1030
Fragments arranged by size:
1030 861 747 516 432 268
EcoO109I rG'GnC_Cy
Cuts at: 993 993
Size: 3854
EcoRI G'AATT_C
Cuts at: 532 730 858 532
Size: 198 128 3528
Fragments arranged by size:
3528 198 128
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1873 1873
Size: 3854
FspI TGC'GCA
Cuts at: 1635 1635
Size: 3854
HaeII r_GCGC'y
Cuts at: 1288 1536 2558 3537 1288
Size: 248 1022 979 1605
Fragments arranged by size:
1605 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1696 1726 1728 1758 1696
Size: 30 2 30 3792
Fragments arranged by size:
3792 30 30 2
HincII GTy'rAC
Cuts at: 264 893 264
Size: 629 3225
KasI G'GCGC_C
Cuts at: 1284 1532 1284
Size: 248 3606
KpnI G_GTAC'C
Cuts at: 311 405 874 311
Size: 94 469 3291
Fragments arranged by size:
3291 469 94
MluI A'CGCG_T
Cuts at: 3287 3287
Size: 3854
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2525 2709 2525
Size: 184 3670
MscI TGG'CCA
Cuts at: 1615 1615
Size: 3854
MslI CAynn'nnrTG
Cuts at: 133 1843 1970 133
Size: 1710 127 2017
Fragments arranged by size:
2017 1710 127
MspA1I CmG'CkG
Cuts at: 965 1279 1639 2652 2897 965
Size: 314 360 1013 245 1922
Fragments arranged by size:
1922 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2035 2035
Size: 3854
NarI GG'CG_CC
Cuts at: 1285 1533 1285
Size: 248 3606
NgoMIV G'CCGG_C
Cuts at: 2033 2033
Size: 3854
NspI r_CATG'y
Cuts at: 907 1407 2314 907
Size: 500 907 2447
Fragments arranged by size:
2447 907 500
PciI A'CATG_T
Cuts at: 2310 2310
Size: 3854
PmeI GTTT'AAAC
Cuts at: 853 853
Size: 3854
PmlI CAC'GTG
Cuts at: 791 791
Size: 3854
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2993 3025 2993
Size: 32 3822
PstI C_TGCA'G
Cuts at: 901 1586 901
Size: 685 3169
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3854
PvuII CAG'CTG
Cuts at: 1279 1639 1279
Size: 360 3494
RsrII CG'GwC_CG
Cuts at: 2049 2049
Size: 3854
SacI G_AGCT'C
Cuts at: 868 868
Size: 3854
SalI G'TCGA_C
Cuts at: 891 891
Size: 3854
SapI GCTCTTCn'nnn_
Cuts at: 1877 2087 1877
Size: 210 3644
SbfI CC_TGCA'GG
Cuts at: 901 901
Size: 3854
ScaI AGT'ACT
Cuts at: 3371 3371
Size: 3854
SfcI C'TryA_G
Cuts at: 33 897 1582 2575 2766 33
Size: 864 685 993 191 1121
Fragments arranged by size:
1121 993 864 685 191
SfoI GGC'GCC
Cuts at: 1286 1534 1286
Size: 248 3606
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3854
SmaI CCC'GGG
Cuts at: 876 876
Size: 3854
SmlI C'TyrA_G
Cuts at: 131 1009 1924 2416 2678 131
Size: 878 915 492 262 1307
Fragments arranged by size:
1307 915 878 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3854
SphI G_CATG'C
Cuts at: 907 1407 907
Size: 500 3354
StuI AGG'CCT
Cuts at: 116 116
Size: 3854
StyI C'CwwG_G
Cuts at: 397 988 1342 397
Size: 591 354 2909
Fragments arranged by size:
2909 591 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1437 1570 2029 2238 1437
Size: 133 459 209 3053
Fragments arranged by size:
3053 459 209 133
TatI w'GTAC_w
Cuts at: 422 3369 422
Size: 2947 907
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3371 3403 3371
Size: 32 3822
Tth111I GACn'n_nGTC
Cuts at: 1651 1651
Size: 3854
XbaI T'CTAG_A
Cuts at: 885 885
Size: 3854
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1349 1349
Size: 3854
XmaI C'CCGG_G
Cuts at: 874 874
Size: 3854
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PmlI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BpmI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI