Restriction map for
pF1KB8317
(Circular) (Six-base) MAPSORT of: pF1KB8317.seq Check: 7408 from: 1 to: 4571
pF1KB8317 4571 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 720 720
Size: 4571
AccI GT'mk_AC
Cuts at: 1609 1609
Size: 4571
Acc65I G'GTAC_C
Cuts at: 1587 1587
Size: 4571
AclI AA'CG_TT
Cuts at: 4411 4411
Size: 4571
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 1135 2396 2828 3575 3843 431
Size: 704 1261 432 747 268 1159
Fragments arranged by size:
1261 1159 747 704 432 268
AflII C'TTAA_G
Cuts at: 430 430
Size: 4571
AflIII A'CryG_T
Cuts at: 1923 3027 4004 1923
Size: 1104 977 2490
Fragments arranged by size:
2490 1104 977
AgeI A'CCGG_T
Cuts at: 2959 2959
Size: 4571
AlwNI CAG_nnn'CTG
Cuts at: 658 943 1343 1876 3443 658
Size: 285 400 533 1567 1786
Fragments arranged by size:
1786 1567 533 400 285
ApaLI G'TGCA_C
Cuts at: 3341 3341
Size: 4571
ApoI r'AATT_y
Cuts at: 1575 3924 4180 1575
Size: 2349 256 1966
Fragments arranged by size:
2349 1966 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4571
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4571
AvaI C'yCGr_G
Cuts at: 1031 1285 1591 1031
Size: 254 306 4011
Fragments arranged by size:
4011 306 254
BamHI G'GATC_C
Cuts at: 1596 1596
Size: 4571
BanI G'GyrC_C
Cuts at: 691 1587 2001 2249 2284 691
Size: 896 414 248 35 2978
Fragments arranged by size:
2978 896 414 248 35
BanII G_rGCy'C
Cuts at: 131 242 275 601 1132 1286 1294 1322 1585
Size: 111 33 326 531 154 8 28 263
Cuts at: 1585 2615 131
Size: 1030 2087
Fragments arranged by size:
2087 1030 531 326 263 154 111 33
28 8
BbeI G_GCGC'C
Cuts at: 2005 2253 2005
Size: 248 4323
BbsI GAAGACnn'nnnn_
Cuts at: 155 971 1123 155
Size: 816 152 3603
Fragments arranged by size:
3603 816 152
BbvCI CC'TCA_GC
Cuts at: 809 878 809
Size: 69 4502
BciVI GTATCCnnnnn_n'
Cuts at: 2469 3230 4571 2469
Size: 761 1341 2469
Fragments arranged by size:
2469 1341 761
BclI T'GATC_A
Cuts at: 479 848 2091 479
Size: 369 1243 2959
Fragments arranged by size:
2959 1243 369
BglI GCCn_nnn'nGGC
Cuts at: 952 1441 952
Size: 489 4082
BglII A'GATC_T
Cuts at: 2086 4123 2086
Size: 2037 2534
BlpI GC'TnA_GC
Cuts at: 269 1683 269
Size: 1414 3157
Bme1580I G_kGCm'C
Cuts at: 2196 2289 3345 2196
Size: 93 1056 3422
Fragments arranged by size:
3422 1056 93
BmgBI CAC'GTC
Cuts at: 716 716
Size: 4571
BmrI ACTGGGnnnn_n'
Cuts at: 596 1783 1864 2199 4343 596
Size: 1187 81 335 2144 824
Fragments arranged by size:
2144 1187 824 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2938 2938
Size: 4571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 635 766 1399 145
Size: 490 131 633 3317
Fragments arranged by size:
3317 633 490 131
Bpu10I CC'TnA_GC
Cuts at: 130 809 878 1280 1316 130
Size: 679 69 402 36 3385
Fragments arranged by size:
3385 679 402 69 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 169 1350 1747 2626 3118 3416 154
Size: 15 1181 397 879 492 298 1309
Fragments arranged by size:
1309 1181 879 492 397 298 15
BsaI GGTCTCn'nnnn_
Cuts at: 693 994 4275 693
Size: 301 3281 989
Fragments arranged by size:
3281 989 301
BsaAI yAC'GTr
Cuts at: 1924 1924
Size: 4571
BsaBI GATnn'nnATC
Cuts at: 6 2085 4122 6
Size: 2079 2037 455
Fragments arranged by size:
2079 2037 455
BsaHI Gr'CG_yC
Cuts at: 675 1543 2002 2250 2952 4448 675
Size: 868 459 248 702 1496 798
Fragments arranged by size:
1496 868 798 702 459 248
BsaWI w'CCGG_w
Cuts at: 1760 2281 2959 3233 3380 3791 1760
Size: 521 678 274 147 411 2540
Fragments arranged by size:
2540 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1173 1366 1382 1537 1173
Size: 193 16 155 4207
Fragments arranged by size:
4207 193 155 16
BseYI C'CCAG_C
Cuts at: 115 457 1996 2754 3331 3827 115
Size: 342 1539 758 577 496 859
Fragments arranged by size:
1539 859 758 577 496 342
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 748 1015 265
Size: 483 267 3821
Fragments arranged by size:
3821 483 267
BsiEI CG_ry'CG
Cuts at: 104 1445 2113 2159 3367 104
Size: 1341 668 46 1208 1308
Fragments arranged by size:
1341 1308 1208 668 46
BsiHKAI G_wGCw'C
Cuts at: 1205 1294 1585 2363 2553 3345 1205
Size: 89 291 778 190 792 2431
Fragments arranged by size:
2431 792 778 291 190 89
BsmBI CGTCTCn'nnnn_
Cuts at: 1030 1030
Size: 4571
Bsp1286I G_dGCh'C
Cuts at: 131 242 275 601 1132 1205 1286 1294 1322
Size: 111 33 326 531 73 81 8 28
Cuts at: 1322 1585 2196 2289 2363 2553 2615 3345 131
Size: 263 611 93 74 190 62 730 1357
Fragments arranged by size:
1357 730 611 531 326 263 190 111
93 81 74 73 62 33 28 8
BspEI T'CCGG_A
Cuts at: 3791 3791
Size: 4571
BspHI T'CATG_A
Cuts at: 3747 3747
Size: 4571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 720 1621 1835 2137 2518 720
Size: 901 214 302 381 2773
Fragments arranged by size:
2773 901 381 302 214
BsrBI CCG'CTC
Cuts at: 50 452 689 1103 2863 2917 4401 4566 50
Size: 402 237 414 1760 54 1484 165 55
Fragments arranged by size:
1760 1484 414 402 237 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2483 2483
Size: 4571
BsrFI r'CCGG_y
Cuts at: 2569 2750 2959 2569
Size: 181 209 4181
Fragments arranged by size:
4181 209 181
BssSI C'ACGA_G
Cuts at: 696 2842 3200 696
Size: 2146 358 2067
Fragments arranged by size:
2146 2067 358
BstAPI GCAn_nnn'nTGC
Cuts at: 943 1343 1948 943
Size: 400 605 3566
Fragments arranged by size:
3566 605 400
BstBI TT'CG_AA
Cuts at: 533 2932 533
Size: 2399 2172
BstYI r'GATC_y
Cuts at: 1 1596 2063 2086 2421 2667 3679 3765 3777
Size: 1595 467 23 335 246 1012 86 12
Cuts at: 3777 4123 1
Size: 346 449
Fragments arranged by size:
1595 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 890 890
Size: 4571
BtgI C'CryG_G
Cuts at: 107 1421 1439 107
Size: 1314 18 3239
Fragments arranged by size:
3239 1314 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2521 2702 2865 2521
Size: 181 163 4227
Fragments arranged by size:
4227 181 163
BtsI GCAGTG_nn'
Cuts at: 1249 1814 1249
Size: 565 4006
DraI TTT'AAA
Cuts at: 192 1570 192
Size: 1378 3193
DrdI GACnn_nn'nnGTC
Cuts at: 2277 3135 2277
Size: 858 3713
EaeI y'GGCC_r
Cuts at: 447 710 1442 2156 2330 2721 2748 4093 447
Size: 263 732 714 174 391 27 1345 925
Fragments arranged by size:
1345 925 732 714 391 263 174 27
EagI C'GGCC_G
Cuts at: 1442 2156 1442
Size: 714 3857
EarI CTCTTCn'nnn_
Cuts at: 212 1093 2594 2804 212
Size: 881 1501 210 1979
Fragments arranged by size:
1979 1501 881 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 306 1152 2779 3089 3235 4381 306
Size: 846 1627 310 146 1146 496
Fragments arranged by size:
1627 1146 846 496 310 146
EcoICRI GAG'CTC
Cuts at: 1292 1583 1292
Size: 291 4280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 431 635 766 1135 1399 2396 2828 3575
Size: 286 204 131 369 264 997 432 747
Cuts at: 3575 3843 145
Size: 268 873
Fragments arranged by size:
997 873 747 432 369 286 268 264
204 131
EcoO109I rG'GnC_Cy
Cuts at: 156 165 382 741 1239 1299 1710 156
Size: 9 217 359 498 60 411 3017
Fragments arranged by size:
3017 498 411 359 217 60 9
EcoRI G'AATT_C
Cuts at: 1575 1575
Size: 4571
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2590 2590
Size: 4571
FspI TGC'GCA
Cuts at: 2352 2352
Size: 4571
HaeII r_GCGC'y
Cuts at: 363 1181 2005 2253 3275 4254 363
Size: 818 824 248 1022 979 680
Fragments arranged by size:
1022 979 824 818 680 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2413 2443 2445 2475 2413
Size: 30 2 30 4509
Fragments arranged by size:
4509 30 30 2
HincII GTy'rAC
Cuts at: 1610 1610
Size: 4571
HindIII A'AGCT_T
Cuts at: 335 427 335
Size: 92 4479
KasI G'GCGC_C
Cuts at: 2001 2249 2001
Size: 248 4323
KpnI G_GTAC'C
Cuts at: 1591 1591
Size: 4571
MluI A'CGCG_T
Cuts at: 4004 4004
Size: 4571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3242 3426 3242
Size: 184 4387
MscI TGG'CCA
Cuts at: 2332 2332
Size: 4571
MslI CAynn'nnrTG
Cuts at: 2560 2687 2560
Size: 127 4444
MspA1I CmG'CkG
Cuts at: 357 943 1423 1682 1996 2356 3369 3614 357
Size: 586 480 259 314 360 1013 245 1314
Fragments arranged by size:
1314 1013 586 480 360 314 259 245
NaeI GCC'GGC
Cuts at: 2752 2752
Size: 4571
NarI GG'CG_CC
Cuts at: 2002 2250 2002
Size: 248 4323
NcoI C'CATG_G
Cuts at: 107 107
Size: 4571
NgoMIV G'CCGG_C
Cuts at: 2750 2750
Size: 4571
NspI r_CATG'y
Cuts at: 1624 2124 3031 1624
Size: 500 907 3164
Fragments arranged by size:
3164 907 500
PasI CC'CwG_GG
Cuts at: 489 1132 489
Size: 643 3928
PciI A'CATG_T
Cuts at: 3027 3027
Size: 4571
PflMI CCAn_nnn'nTGG
Cuts at: 658 843 658
Size: 185 4386
PfoI T'CCnGG_A
Cuts at: 651 743 651
Size: 92 4479
PmeI GTTT'AAAC
Cuts at: 1570 1570
Size: 4571
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 3710
Size: 32 4539
PpuMI rG'GwC_Cy
Cuts at: 156 382 741 1299 156
Size: 226 359 558 3428
Fragments arranged by size:
3428 558 359 226
PstI C_TGCA'G
Cuts at: 1618 2303 1618
Size: 685 3886
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4571
PvuII CAG'CTG
Cuts at: 1996 2356 1996
Size: 360 4211
RsrII CG'GwC_CG
Cuts at: 2766 2766
Size: 4571
SacI G_AGCT'C
Cuts at: 1294 1585 1294
Size: 291 4280
SacII CC_GC'GG
Cuts at: 1424 1424
Size: 4571
SalI G'TCGA_C
Cuts at: 1608 1608
Size: 4571
SanDI GG'GwC_CC
Cuts at: 382 382
Size: 4571
SapI GCTCTTCn'nnn_
Cuts at: 1093 2594 2804 1093
Size: 1501 210 2860
Fragments arranged by size:
2860 1501 210
SbfI CC_TGCA'GG
Cuts at: 1618 1618
Size: 4571
ScaI AGT'ACT
Cuts at: 4088 4088
Size: 4571
SfcI C'TryA_G
Cuts at: 33 1614 2299 3292 3483 33
Size: 1581 685 993 191 1121
Fragments arranged by size:
1581 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2003 2251 2003
Size: 248 4323
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4571
SmaI CCC'GGG
Cuts at: 1033 1593 1033
Size: 560 4011
SmlI C'TyrA_G
Cuts at: 169 184 430 1329 1726 2641 3133 3395 169
Size: 15 246 899 397 915 492 262 1345
Fragments arranged by size:
1345 915 899 492 397 262 246 15
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4571
SphI G_CATG'C
Cuts at: 1624 2124 1624
Size: 500 4071
StyI C'CwwG_G
Cuts at: 107 704 1160 1397 1507 1705 2059 107
Size: 597 456 237 110 198 354 2619
Fragments arranged by size:
2619 597 456 354 237 198 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 850 1567 2154 2287 2746 2955 850
Size: 717 587 133 459 209 2466
Fragments arranged by size:
2466 717 587 459 209 133
TatI w'GTAC_w
Cuts at: 1454 4086 1454
Size: 2632 1939
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1213 1245 4088 4120 1213
Size: 32 2843 32 1664
Fragments arranged by size:
2843 1664 32 32
Tth111I GACn'n_nGTC
Cuts at: 2368 2368
Size: 4571
XbaI T'CTAG_A
Cuts at: 1602 1602
Size: 4571
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 840 2066 840
Size: 1226 3345
XmaI C'CCGG_G
Cuts at: 1031 1591 1031
Size: 560 4011
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmtI BsaXI BsiWI BsmI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspOMI
PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI