Restriction map for
pF1KB8315
(Circular) (Six-base) MAPSORT of: pF1KB8315.seq Check: 2119 from: 1 to: 3875
pF1KB8315 3875 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 913 913
Size: 3875
Acc65I G'GTAC_C
Cuts at: 891 891
Size: 3875
AclI AA'CG_TT
Cuts at: 3715 3715
Size: 3875
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 872 1700 2132 2879 3147 557
Size: 315 828 432 747 268 1285
Fragments arranged by size:
1285 828 747 432 315 268
AflIII A'CryG_T
Cuts at: 1227 2331 3308 1227
Size: 1104 977 1794
Fragments arranged by size:
1794 1104 977
AgeI A'CCGG_T
Cuts at: 2263 2263
Size: 3875
AlwNI CAG_nnn'CTG
Cuts at: 487 795 1180 2747 487
Size: 308 385 1567 1615
Fragments arranged by size:
1615 1567 385 308
ApaLI G'TGCA_C
Cuts at: 2645 2645
Size: 3875
ApoI r'AATT_y
Cuts at: 300 879 3228 3484 300
Size: 579 2349 256 691
Fragments arranged by size:
2349 691 579 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3875
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
AvaI C'yCGr_G
Cuts at: 430 895 430
Size: 465 3410
BamHI G'GATC_C
Cuts at: 900 900
Size: 3875
BanI G'GyrC_C
Cuts at: 891 1305 1553 1588 891
Size: 414 248 35 3178
Fragments arranged by size:
3178 414 248 35
BanII G_rGCy'C
Cuts at: 431 634 889 1919 431
Size: 203 255 1030 2387
Fragments arranged by size:
2387 1030 255 203
BbeI G_GCGC'C
Cuts at: 1309 1557 1309
Size: 248 3627
BbsI GAAGACnn'nnnn_
Cuts at: 521 521
Size: 3875
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 110 144 849 883 110
Size: 34 705 34 3102
Fragments arranged by size:
3102 705 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 126 1773 2534 3875 126
Size: 1647 761 1341 126
Fragments arranged by size:
1647 1341 761 126
BclI T'GATC_A
Cuts at: 1395 1395
Size: 3875
BglII A'GATC_T
Cuts at: 123 218 1390 3427 123
Size: 95 1172 2037 571
Fragments arranged by size:
2037 1172 571 95
BlpI GC'TnA_GC
Cuts at: 987 987
Size: 3875
Bme1580I G_kGCm'C
Cuts at: 1500 1593 2649 1500
Size: 93 1056 2726
Fragments arranged by size:
2726 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1087 1168 1503 3647 1087
Size: 81 335 2144 1315
Fragments arranged by size:
2144 1315 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 3875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 609 609
Size: 3875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1051 1930 2422 2720 1051
Size: 879 492 298 2206
Fragments arranged by size:
2206 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 226 3579 226
Size: 3353 522
BsaAI yAC'GTr
Cuts at: 1228 1228
Size: 3875
BsaBI GATnn'nnATC
Cuts at: 6 518 1389 3426 6
Size: 512 871 2037 455
Fragments arranged by size:
2037 871 512 455
BsaHI Gr'CG_yC
Cuts at: 1306 1554 2256 3752 1306
Size: 248 702 1496 1429
Fragments arranged by size:
1496 1429 702 248
BsaWI w'CCGG_w
Cuts at: 1064 1585 2263 2537 2684 3095 1064
Size: 521 678 274 147 411 1844
Fragments arranged by size:
1844 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 418 448 418
Size: 30 3845
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 267 556 654 675 267
Size: 289 98 21 3467
Fragments arranged by size:
3467 289 98 21
BseYI C'CCAG_C
Cuts at: 259 751 756 1300 2058 2635 3131 259
Size: 492 5 544 758 577 496 1003
Fragments arranged by size:
1003 758 577 544 496 492 5
BsiEI CG_ry'CG
Cuts at: 104 576 1417 1463 2671 104
Size: 472 841 46 1208 1308
Fragments arranged by size:
1308 1208 841 472 46
BsiHKAI G_wGCw'C
Cuts at: 634 744 889 1667 1857 2649 634
Size: 110 145 778 190 792 1860
Fragments arranged by size:
1860 792 778 190 145 110
Bsp1286I G_dGCh'C
Cuts at: 431 634 744 889 1500 1593 1667 1857 1919
Size: 203 110 145 611 93 74 190 62
Cuts at: 1919 2649 431
Size: 730 1657
Fragments arranged by size:
1657 730 611 203 190 145 110 93
74 62
BspEI T'CCGG_A
Cuts at: 3095 3095
Size: 3875
BspHI T'CATG_A
Cuts at: 3051 3051
Size: 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 925 1139 1441 1822 925
Size: 214 302 381 2978
Fragments arranged by size:
2978 381 302 214
BsrBI CCG'CTC
Cuts at: 50 780 2167 2221 3705 3870 50
Size: 730 1387 54 1484 165 55
Fragments arranged by size:
1484 1387 730 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1787 1787
Size: 3875
BsrFI r'CCGG_y
Cuts at: 158 439 1873 2054 2263 158
Size: 281 1434 181 209 1770
Fragments arranged by size:
1770 1434 281 209 181
BssSI C'ACGA_G
Cuts at: 2146 2504 2146
Size: 358 3517
BstAPI GCAn_nnn'nTGC
Cuts at: 1252 1252
Size: 3875
BstBI TT'CG_AA
Cuts at: 2236 2236
Size: 3875
BstYI r'GATC_y
Cuts at: 1 123 218 900 1367 1390 1725 1971 2983
Size: 122 95 682 467 23 335 246 1012
Cuts at: 2983 3069 3081 3427 1
Size: 86 12 346 449
Fragments arranged by size:
1012 682 467 449 346 335 246 122
95 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3875
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1825 2006 2169 1825
Size: 181 163 3531
Fragments arranged by size:
3531 181 163
BtsI GCAGTG_nn'
Cuts at: 1118 1118
Size: 3875
DraI TTT'AAA
Cuts at: 874 874
Size: 3875
DraIII CAC_nnn'GTG
Cuts at: 507 507
Size: 3875
DrdI GACnn_nn'nnGTC
Cuts at: 1581 2439 1581
Size: 858 3017
EaeI y'GGCC_r
Cuts at: 573 1460 1634 2025 2052 3397 573
Size: 887 174 391 27 1345 1051
Fragments arranged by size:
1345 1051 887 391 174 27
EagI C'GGCC_G
Cuts at: 573 1460 573
Size: 887 2988
EarI CTCTTCn'nnn_
Cuts at: 389 533 705 829 1898 2108 389
Size: 144 172 124 1069 210 2156
Fragments arranged by size:
2156 1069 210 172 144 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 258 448 2083 2393 2539 3685 258
Size: 190 1635 310 146 1146 448
Fragments arranged by size:
1635 1146 448 310 190 146
EcoICRI GAG'CTC
Cuts at: 632 887 632
Size: 255 3620
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 609 872 1700 2132 2879 3147 557
Size: 52 263 828 432 747 268 1285
Fragments arranged by size:
1285 828 747 432 268 263 52
EcoNI CCTnn'n_nnAGG
Cuts at: 185 185
Size: 3875
EcoO109I rG'GnC_Cy
Cuts at: 774 1014 774
Size: 240 3635
EcoRI G'AATT_C
Cuts at: 300 879 300
Size: 579 3296
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 237 1894 237
Size: 1657 2218
FspI TGC'GCA
Cuts at: 1656 1656
Size: 3875
HaeII r_GCGC'y
Cuts at: 1309 1557 2579 3558 1309
Size: 248 1022 979 1626
Fragments arranged by size:
1626 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 418 450 511 543 1717 1747 1749 1779 418
Size: 32 61 32 1174 30 2 30 2514
Fragments arranged by size:
2514 1174 61 32 32 30 30 2
HincII GTy'rAC
Cuts at: 914 914
Size: 3875
KasI G'GCGC_C
Cuts at: 1305 1553 1305
Size: 248 3627
KpnI G_GTAC'C
Cuts at: 895 895
Size: 3875
MluI A'CGCG_T
Cuts at: 3308 3308
Size: 3875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2546 2730 2546
Size: 184 3691
MscI TGG'CCA
Cuts at: 1636 1636
Size: 3875
MslI CAynn'nnrTG
Cuts at: 1864 1991 1864
Size: 127 3748
MspA1I CmG'CkG
Cuts at: 792 986 1300 1660 2673 2918 792
Size: 194 314 360 1013 245 1749
Fragments arranged by size:
1749 1013 360 314 245 194
NaeI GCC'GGC
Cuts at: 441 2056 441
Size: 1615 2260
NarI GG'CG_CC
Cuts at: 1306 1554 1306
Size: 248 3627
NcoI C'CATG_G
Cuts at: 107 107
Size: 3875
NgoMIV G'CCGG_C
Cuts at: 439 2054 439
Size: 1615 2260
NspI r_CATG'y
Cuts at: 928 1428 2335 928
Size: 500 907 2468
Fragments arranged by size:
2468 907 500
PciI A'CATG_T
Cuts at: 2331 2331
Size: 3875
PfoI T'CCnGG_A
Cuts at: 625 625
Size: 3875
PmeI GTTT'AAAC
Cuts at: 874 874
Size: 3875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3014 3046 3014
Size: 32 3843
PpuMI rG'GwC_Cy
Cuts at: 774 774
Size: 3875
PstI C_TGCA'G
Cuts at: 922 1607 922
Size: 685 3190
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3875
PvuII CAG'CTG
Cuts at: 792 1300 1660 792
Size: 508 360 3007
Fragments arranged by size:
3007 508 360
RsrII CG'GwC_CG
Cuts at: 2070 2070
Size: 3875
SacI G_AGCT'C
Cuts at: 634 889 634
Size: 255 3620
SalI G'TCGA_C
Cuts at: 912 912
Size: 3875
SapI GCTCTTCn'nnn_
Cuts at: 1898 2108 1898
Size: 210 3665
SbfI CC_TGCA'GG
Cuts at: 922 922
Size: 3875
ScaI AGT'ACT
Cuts at: 3392 3392
Size: 3875
SexAI A'CCwGG_T
Cuts at: 503 503
Size: 3875
SfcI C'TryA_G
Cuts at: 33 182 918 1603 2596 2787 33
Size: 149 736 685 993 191 1121
Fragments arranged by size:
1121 993 736 685 191 149
SfoI GGC'GCC
Cuts at: 1307 1555 1307
Size: 248 3627
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
SmaI CCC'GGG
Cuts at: 897 897
Size: 3875
SmlI C'TyrA_G
Cuts at: 1030 1945 2437 2699 1030
Size: 915 492 262 2206
Fragments arranged by size:
2206 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3875
SphI G_CATG'C
Cuts at: 928 1428 928
Size: 500 3375
StyI C'CwwG_G
Cuts at: 107 1009 1363 107
Size: 902 354 2619
Fragments arranged by size:
2619 902 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1458 1591 2050 2259 1458
Size: 133 459 209 3074
Fragments arranged by size:
3074 459 209 133
TatI w'GTAC_w
Cuts at: 3390 3390
Size: 3875
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3392 3424 3392
Size: 32 3843
Tth111I GACn'n_nGTC
Cuts at: 1672 1672
Size: 3875
XbaI T'CTAG_A
Cuts at: 906 906
Size: 3875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 633 1370 633
Size: 737 3138
XmaI C'CCGG_G
Cuts at: 895 895
Size: 3875
XmnI GAAnn'nnTTC
Cuts at: 220 220
Size: 3875
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI