Restriction map for
pF1KB8311
(Circular) (Six-base) MAPSORT of: pF1KB8311.seq Check: 4822 from: 1 to: 3464
pF1KB8311 3464 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 502 502
Size: 3464
Acc65I G'GTAC_C
Cuts at: 480 480
Size: 3464
AclI AA'CG_TT
Cuts at: 3304 3304
Size: 3464
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1289 1721 2468 2736 1289
Size: 432 747 268 2017
Fragments arranged by size:
2017 747 432 268
AflIII A'CryG_T
Cuts at: 816 1920 2897 816
Size: 1104 977 1383
Fragments arranged by size:
1383 1104 977
AgeI A'CCGG_T
Cuts at: 1852 1852
Size: 3464
AlwNI CAG_nnn'CTG
Cuts at: 769 2336 769
Size: 1567 1897
ApaLI G'TGCA_C
Cuts at: 2234 2234
Size: 3464
ApoI r'AATT_y
Cuts at: 468 2817 3073 468
Size: 2349 256 859
Fragments arranged by size:
2349 859 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3464
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3464
AvaI C'yCGr_G
Cuts at: 484 484
Size: 3464
BamHI G'GATC_C
Cuts at: 308 489 308
Size: 181 3283
BanI G'GyrC_C
Cuts at: 480 894 1142 1177 480
Size: 414 248 35 2767
Fragments arranged by size:
2767 414 248 35
BanII G_rGCy'C
Cuts at: 478 1508 478
Size: 1030 2434
BbeI G_GCGC'C
Cuts at: 898 1146 898
Size: 248 3216
BciVI GTATCCnnnnn_n'
Cuts at: 1362 2123 3464 1362
Size: 761 1341 1362
Fragments arranged by size:
1362 1341 761
BclI T'GATC_A
Cuts at: 984 984
Size: 3464
BglII A'GATC_T
Cuts at: 979 3016 979
Size: 2037 1427
BlpI GC'TnA_GC
Cuts at: 576 576
Size: 3464
Bme1580I G_kGCm'C
Cuts at: 1089 1182 2238 1089
Size: 93 1056 2315
Fragments arranged by size:
2315 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 676 757 1092 3236 676
Size: 81 335 2144 904
Fragments arranged by size:
2144 904 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 150 1831 150
Size: 1681 1783
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 1519 2011 2309 640
Size: 879 492 298 1795
Fragments arranged by size:
1795 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 219 3168 219
Size: 2949 515
BsaAI yAC'GTr
Cuts at: 817 817
Size: 3464
BsaBI GATnn'nnATC
Cuts at: 6 978 3015 6
Size: 972 2037 455
Fragments arranged by size:
2037 972 455
BsaHI Gr'CG_yC
Cuts at: 895 1143 1845 3341 895
Size: 248 702 1496 1018
Fragments arranged by size:
1496 1018 702 248
BsaWI w'CCGG_w
Cuts at: 195 653 1174 1852 2126 2273 2684 195
Size: 458 521 678 274 147 411 975
Fragments arranged by size:
975 678 521 458 411 274 147
BseYI C'CCAG_C
Cuts at: 889 1647 2224 2720 889
Size: 758 577 496 1633
Fragments arranged by size:
1633 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1006 1052 2260 104
Size: 902 46 1208 1308
Fragments arranged by size:
1308 1208 902 46
BsiHKAI G_wGCw'C
Cuts at: 478 1256 1446 2238 478
Size: 778 190 792 1704
Fragments arranged by size:
1704 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 478 1089 1182 1256 1446 1508 2238 478
Size: 611 93 74 190 62 730 1704
Fragments arranged by size:
1704 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2684 2684
Size: 3464
BspHI T'CATG_A
Cuts at: 2640 2640
Size: 3464
BspMI ACCTGCnnnn'nnnn_
Cuts at: 514 728 1030 1411 514
Size: 214 302 381 2567
Fragments arranged by size:
2567 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1756 1810 3294 3459 50
Size: 1706 54 1484 165 55
Fragments arranged by size:
1706 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1376 1376
Size: 3464
BsrFI r'CCGG_y
Cuts at: 1462 1643 1852 1462
Size: 181 209 3074
Fragments arranged by size:
3074 209 181
BssSI C'ACGA_G
Cuts at: 1735 2093 1735
Size: 358 3106
BstAPI GCAn_nnn'nTGC
Cuts at: 841 841
Size: 3464
BstBI TT'CG_AA
Cuts at: 1825 1825
Size: 3464
BstYI r'GATC_y
Cuts at: 1 308 489 956 979 1314 1560 2572 2658
Size: 307 181 467 23 335 246 1012 86
Cuts at: 2658 2670 3016 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 307 246 181
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1414 1595 1758 1414
Size: 181 163 3120
Fragments arranged by size:
3120 181 163
BtsI GCAGTG_nn'
Cuts at: 707 707
Size: 3464
DraI TTT'AAA
Cuts at: 463 463
Size: 3464
DrdI GACnn_nn'nnGTC
Cuts at: 1170 2028 1170
Size: 858 2606
EaeI y'GGCC_r
Cuts at: 1049 1223 1614 1641 2986 1049
Size: 174 391 27 1345 1527
Fragments arranged by size:
1527 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1049 1049
Size: 3464
EarI CTCTTCn'nnn_
Cuts at: 1487 1697 1487
Size: 210 3254
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1672 1982 2128 3274 1672
Size: 310 146 1146 1862
Fragments arranged by size:
1862 1146 310 146
EcoICRI GAG'CTC
Cuts at: 476 476
Size: 3464
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1289 1721 2468 2736 1289
Size: 432 747 268 2017
Fragments arranged by size:
2017 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 603 603
Size: 3464
EcoRI G'AATT_C
Cuts at: 468 468
Size: 3464
EcoRV GAT'ATC
Cuts at: 270 270
Size: 3464
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 351 1483 351
Size: 1132 2332
FspI TGC'GCA
Cuts at: 1245 1245
Size: 3464
HaeII r_GCGC'y
Cuts at: 898 1146 2168 3147 898
Size: 248 1022 979 1215
Fragments arranged by size:
1215 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1306 1336 1338 1368 1306
Size: 30 2 30 3402
Fragments arranged by size:
3402 30 30 2
HincII GTy'rAC
Cuts at: 234 503 234
Size: 269 3195
KasI G'GCGC_C
Cuts at: 894 1142 894
Size: 248 3216
KpnI G_GTAC'C
Cuts at: 484 484
Size: 3464
MluI A'CGCG_T
Cuts at: 2897 2897
Size: 3464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2135 2319 2135
Size: 184 3280
MscI TGG'CCA
Cuts at: 1225 1225
Size: 3464
MslI CAynn'nnrTG
Cuts at: 1453 1580 1453
Size: 127 3337
MspA1I CmG'CkG
Cuts at: 437 575 889 1249 2262 2507 437
Size: 138 314 360 1013 245 1394
Fragments arranged by size:
1394 1013 360 314 245 138
NaeI GCC'GGC
Cuts at: 1645 1645
Size: 3464
NarI GG'CG_CC
Cuts at: 895 1143 895
Size: 248 3216
NgoMIV G'CCGG_C
Cuts at: 1643 1643
Size: 3464
NspI r_CATG'y
Cuts at: 517 1017 1924 517
Size: 500 907 2057
Fragments arranged by size:
2057 907 500
PciI A'CATG_T
Cuts at: 1920 1920
Size: 3464
PflMI CCAn_nnn'nTGG
Cuts at: 113 138 113
Size: 25 3439
PmeI GTTT'AAAC
Cuts at: 463 463
Size: 3464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2603 2635 2603
Size: 32 3432
PstI C_TGCA'G
Cuts at: 511 1196 511
Size: 685 2779
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3464
PvuII CAG'CTG
Cuts at: 889 1249 889
Size: 360 3104
RsrII CG'GwC_CG
Cuts at: 1659 1659
Size: 3464
SacI G_AGCT'C
Cuts at: 478 478
Size: 3464
SalI G'TCGA_C
Cuts at: 501 501
Size: 3464
SapI GCTCTTCn'nnn_
Cuts at: 1487 1697 1487
Size: 210 3254
SbfI CC_TGCA'GG
Cuts at: 511 511
Size: 3464
ScaI AGT'ACT
Cuts at: 253 2981 253
Size: 2728 736
SfcI C'TryA_G
Cuts at: 33 432 507 1192 2185 2376 33
Size: 399 75 685 993 191 1121
Fragments arranged by size:
1121 993 685 399 191 75
SfoI GGC'GCC
Cuts at: 896 1144 896
Size: 248 3216
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3464
SmaI CCC'GGG
Cuts at: 486 486
Size: 3464
SmlI C'TyrA_G
Cuts at: 619 1534 2026 2288 619
Size: 915 492 262 1795
Fragments arranged by size:
1795 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3464
SphI G_CATG'C
Cuts at: 517 1017 517
Size: 500 2964
StyI C'CwwG_G
Cuts at: 598 952 598
Size: 354 3110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1047 1180 1639 1848 1047
Size: 133 459 209 2663
Fragments arranged by size:
2663 459 209 133
TatI w'GTAC_w
Cuts at: 251 2979 251
Size: 2728 736
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2981 3013 2981
Size: 32 3432
Tth111I GACn'n_nGTC
Cuts at: 1261 1261
Size: 3464
XbaI T'CTAG_A
Cuts at: 495 495
Size: 3464
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 375 959 375
Size: 584 2880
XmaI C'CCGG_G
Cuts at: 484 484
Size: 3464
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI