Restriction map for
pF1KB8310
(Circular) (Six-base) MAPSORT of: pF1KB8310.seq Check: 5663 from: 1 to: 4322
pF1KB8310 4322 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 725 1360 725
Size: 635 3687
Acc65I G'GTAC_C
Cuts at: 1338 1338
Size: 4322
AclI AA'CG_TT
Cuts at: 4162 4162
Size: 4322
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 757 2147 2579 3326 3594 757
Size: 1390 432 747 268 1485
Fragments arranged by size:
1485 1390 747 432 268
AflIII A'CryG_T
Cuts at: 177 1674 2778 3755 177
Size: 1497 1104 977 744
Fragments arranged by size:
1497 1104 977 744
AgeI A'CCGG_T
Cuts at: 2710 2710
Size: 4322
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 821 821
Size: 4322
AlwNI CAG_nnn'CTG
Cuts at: 142 411 1627 3194 142
Size: 269 1216 1567 1270
Fragments arranged by size:
1567 1270 1216 269
ApaLI G'TGCA_C
Cuts at: 3092 3092
Size: 4322
ApoI r'AATT_y
Cuts at: 261 1069 1141 1263 1326 3675 3931 261
Size: 808 72 122 63 2349 256 652
Fragments arranged by size:
2349 808 652 256 122 72 63
AseI AT'TA_AT
Cuts at: 20 20
Size: 4322
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4322
AvaI C'yCGr_G
Cuts at: 1342 1342
Size: 4322
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 341 374 341
Size: 33 4289
BamHI G'GATC_C
Cuts at: 1347 1347
Size: 4322
BanI G'GyrC_C
Cuts at: 420 979 1338 1752 2000 2035 420
Size: 559 359 414 248 35 2707
Fragments arranged by size:
2707 559 414 359 248 35
BanII G_rGCy'C
Cuts at: 1336 2366 1336
Size: 1030 3292
BbeI G_GCGC'C
Cuts at: 1756 2004 1756
Size: 248 4074
BbsI GAAGACnn'nnnn_
Cuts at: 506 506
Size: 4322
BciVI GTATCCnnnnn_n'
Cuts at: 2220 2981 4322 2220
Size: 761 1341 2220
Fragments arranged by size:
2220 1341 761
BclI T'GATC_A
Cuts at: 1842 1842
Size: 4322
BfrBI ATG'CAT
Cuts at: 1023 1023
Size: 4322
BglII A'GATC_T
Cuts at: 1837 3874 1837
Size: 2037 2285
BlpI GC'TnA_GC
Cuts at: 239 608 1434 239
Size: 369 826 3127
Fragments arranged by size:
3127 826 369
Bme1580I G_kGCm'C
Cuts at: 1947 2040 3096 1947
Size: 93 1056 3173
Fragments arranged by size:
3173 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1534 1615 1950 4094 1534
Size: 81 335 2144 1762
Fragments arranged by size:
2144 1762 335 81
BmtI G_CTAG'C
Cuts at: 1307 1307
Size: 4322
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 986 2689 986
Size: 1703 2619
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 288 357 634 929 1065 143
Size: 145 69 277 295 136 3400
Fragments arranged by size:
3400 295 277 145 136 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1498 2377 2869 3167 1498
Size: 879 492 298 2653
Fragments arranged by size:
2653 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1108 4026 1108
Size: 2918 1404
BsaAI yAC'GTr
Cuts at: 1675 1675
Size: 4322
BsaBI GATnn'nnATC
Cuts at: 6 1836 3873 6
Size: 1830 2037 455
Fragments arranged by size:
2037 1830 455
BsaHI Gr'CG_yC
Cuts at: 1753 2001 2703 4199 1753
Size: 248 702 1496 1876
Fragments arranged by size:
1876 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1511 2032 2710 2984 3131 3542 1511
Size: 521 678 274 147 411 2291
Fragments arranged by size:
2291 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 454 454
Size: 4322
BseYI C'CCAG_C
Cuts at: 269 1210 1747 2505 3082 3578 269
Size: 941 537 758 577 496 1013
Fragments arranged by size:
1013 941 758 577 537 496
BsiEI CG_ry'CG
Cuts at: 104 1864 1910 3118 104
Size: 1760 46 1208 1308
Fragments arranged by size:
1760 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1336 2114 2304 3096 1336
Size: 778 190 792 2562
Fragments arranged by size:
2562 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1336 1947 2040 2114 2304 2366 3096 1336
Size: 611 93 74 190 62 730 2562
Fragments arranged by size:
2562 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3542 3542
Size: 4322
BspHI T'CATG_A
Cuts at: 3498 3498
Size: 4322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1001 1372 1586 1888 2269 1001
Size: 371 214 302 381 3054
Fragments arranged by size:
3054 381 371 302 214
BsrBI CCG'CTC
Cuts at: 50 2614 2668 4152 4317 50
Size: 2564 54 1484 165 55
Fragments arranged by size:
2564 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2234 2234
Size: 4322
BsrFI r'CCGG_y
Cuts at: 315 2320 2501 2710 315
Size: 2005 181 209 1927
Fragments arranged by size:
2005 1927 209 181
BssSI C'ACGA_G
Cuts at: 184 2593 2951 184
Size: 2409 358 1555
Fragments arranged by size:
2409 1555 358
BstAPI GCAn_nnn'nTGC
Cuts at: 411 1699 411
Size: 1288 3034
BstBI TT'CG_AA
Cuts at: 853 2683 853
Size: 1830 2492
BstYI r'GATC_y
Cuts at: 1 1038 1347 1814 1837 2172 2418 3430 3516
Size: 1037 309 467 23 335 246 1012 86
Cuts at: 3516 3528 3874 1
Size: 12 346 449
Fragments arranged by size:
1037 1012 467 449 346 335 309 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 437 437
Size: 4322
BtgI C'CryG_G
Cuts at: 107 107
Size: 4322
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2272 2453 2616 2272
Size: 181 163 3978
Fragments arranged by size:
3978 181 163
BtsI GCAGTG_nn'
Cuts at: 493 1565 493
Size: 1072 3250
DraI TTT'AAA
Cuts at: 603 1321 603
Size: 718 3604
DrdI GACnn_nn'nnGTC
Cuts at: 2028 2886 2028
Size: 858 3464
EaeI y'GGCC_r
Cuts at: 231 1907 2081 2472 2499 3844 231
Size: 1676 174 391 27 1345 709
Fragments arranged by size:
1676 1345 709 391 174 27
EagI C'GGCC_G
Cuts at: 1907 1907
Size: 4322
EarI CTCTTCn'nnn_
Cuts at: 639 850 2345 2555 639
Size: 211 1495 210 2406
Fragments arranged by size:
2406 1495 211 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2530 2840 2986 4132 2530
Size: 310 146 1146 2720
Fragments arranged by size:
2720 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1334 1334
Size: 4322
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 288 357 634 757 929 1065 2147 2579
Size: 145 69 277 123 172 136 1082 432
Cuts at: 2579 3326 3594 143
Size: 747 268 871
Fragments arranged by size:
1082 871 747 432 277 268 172 145
136 123 69
EcoO109I rG'GnC_Cy
Cuts at: 139 1179 1461 139
Size: 1040 282 3000
Fragments arranged by size:
3000 1040 282
EcoRI G'AATT_C
Cuts at: 1141 1326 1141
Size: 185 4137
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2341 2341
Size: 4322
FspI TGC'GCA
Cuts at: 2103 2103
Size: 4322
HaeII r_GCGC'y
Cuts at: 1756 2004 3026 4005 1756
Size: 248 1022 979 2073
Fragments arranged by size:
2073 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 999 1031 2164 2194 2196 2226 999
Size: 32 1133 30 2 30 3095
Fragments arranged by size:
3095 1133 32 30 30 2
HincII GTy'rAC
Cuts at: 1361 1361
Size: 4322
HindIII A'AGCT_T
Cuts at: 295 295
Size: 4322
KasI G'GCGC_C
Cuts at: 1752 2000 1752
Size: 248 4074
KpnI G_GTAC'C
Cuts at: 1342 1342
Size: 4322
MfeI C'AATT_G
Cuts at: 340 340
Size: 4322
MluI A'CGCG_T
Cuts at: 3755 3755
Size: 4322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 144 997 2993 3177 144
Size: 853 1996 184 1289
Fragments arranged by size:
1996 1289 853 184
MscI TGG'CCA
Cuts at: 233 2083 233
Size: 1850 2472
MslI CAynn'nnrTG
Cuts at: 2311 2438 2311
Size: 127 4195
MspA1I CmG'CkG
Cuts at: 243 612 907 1433 1747 2107 3120 3365 243
Size: 369 295 526 314 360 1013 245 1200
Fragments arranged by size:
1200 1013 526 369 360 314 295 245
NaeI GCC'GGC
Cuts at: 2503 2503
Size: 4322
NarI GG'CG_CC
Cuts at: 1753 2001 1753
Size: 248 4074
NcoI C'CATG_G
Cuts at: 107 107
Size: 4322
NgoMIV G'CCGG_C
Cuts at: 2501 2501
Size: 4322
NheI G'CTAG_C
Cuts at: 1303 1303
Size: 4322
NsiI A_TGCA'T
Cuts at: 1025 1025
Size: 4322
NspI r_CATG'y
Cuts at: 949 1375 1875 2782 949
Size: 426 500 907 2489
Fragments arranged by size:
2489 907 500 426
PciI A'CATG_T
Cuts at: 2778 2778
Size: 4322
PfoI T'CCnGG_A
Cuts at: 958 958
Size: 4322
PmeI GTTT'AAAC
Cuts at: 1321 1321
Size: 4322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3461 3493 3461
Size: 32 4290
PpuMI rG'GwC_Cy
Cuts at: 139 1179 139
Size: 1040 3282
PstI C_TGCA'G
Cuts at: 1369 2054 1369
Size: 685 3637
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4322
PvuII CAG'CTG
Cuts at: 612 907 1747 2107 612
Size: 295 840 360 2827
Fragments arranged by size:
2827 840 360 295
RsrII CG'GwC_CG
Cuts at: 2517 2517
Size: 4322
SacI G_AGCT'C
Cuts at: 1336 1336
Size: 4322
SalI G'TCGA_C
Cuts at: 1359 1359
Size: 4322
SapI GCTCTTCn'nnn_
Cuts at: 2345 2555 2345
Size: 210 4112
SbfI CC_TGCA'GG
Cuts at: 1369 1369
Size: 4322
ScaI AGT'ACT
Cuts at: 1238 3839 1238
Size: 2601 1721
SfcI C'TryA_G
Cuts at: 33 152 1365 2050 3043 3234 33
Size: 119 1213 685 993 191 1121
Fragments arranged by size:
1213 1121 993 685 191 119
SfoI GGC'GCC
Cuts at: 1754 2002 1754
Size: 248 4074
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4322
SmaI CCC'GGG
Cuts at: 1344 1344
Size: 4322
SmlI C'TyrA_G
Cuts at: 1477 2392 2884 3146 1477
Size: 915 492 262 2653
Fragments arranged by size:
2653 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4322
SphI G_CATG'C
Cuts at: 1375 1875 1375
Size: 500 3822
SspI AAT'ATT
Cuts at: 684 684
Size: 4322
StyI C'CwwG_G
Cuts at: 107 1050 1456 1810 107
Size: 943 406 354 2619
Fragments arranged by size:
2619 943 406 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 128 406 923 1905 2038 2497 2706 128
Size: 278 517 982 133 459 209 1744
Fragments arranged by size:
1744 982 517 459 278 209 133
TatI w'GTAC_w
Cuts at: 942 1236 3837 942
Size: 294 2601 1427
Fragments arranged by size:
2601 1427 294
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3839 3871 3839
Size: 32 4290
Tth111I GACn'n_nGTC
Cuts at: 2119 2119
Size: 4322
XbaI T'CTAG_A
Cuts at: 1353 1353
Size: 4322
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1817 1817
Size: 4322
XmaI C'CCGG_G
Cuts at: 1342 1342
Size: 4322
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NsiI NspI PciI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BbvCI BcgI BglI BmgBI Bpu10I BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI NdeI NotI NruI PacI PasI PflMI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI
ZraI