Restriction map for
pF1KB8306
(Circular) (Six-base) MAPSORT of: pF1KB8306.seq Check: 5030 from: 1 to: 3539
pF1KB8306 3539 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 577 577
Size: 3539
Acc65I G'GTAC_C
Cuts at: 126 555 126
Size: 429 3110
AclI AA'CG_TT
Cuts at: 3379 3379
Size: 3539
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 1364 1796 2543 2811 212
Size: 1152 432 747 268 940
Fragments arranged by size:
1152 940 747 432 268
AflIII A'CryG_T
Cuts at: 891 1995 2972 891
Size: 1104 977 1458
Fragments arranged by size:
1458 1104 977
AgeI A'CCGG_T
Cuts at: 1927 1927
Size: 3539
AlwNI CAG_nnn'CTG
Cuts at: 243 844 2411 243
Size: 601 1567 1371
Fragments arranged by size:
1567 1371 601
ApaLI G'TGCA_C
Cuts at: 2309 2309
Size: 3539
ApoI r'AATT_y
Cuts at: 424 543 2892 3148 424
Size: 119 2349 256 815
Fragments arranged by size:
2349 815 256 119
AseI AT'TA_AT
Cuts at: 20 20
Size: 3539
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3539
AvaI C'yCGr_G
Cuts at: 559 559
Size: 3539
BamHI G'GATC_C
Cuts at: 564 564
Size: 3539
BanI G'GyrC_C
Cuts at: 126 555 969 1217 1252 126
Size: 429 414 248 35 2413
Fragments arranged by size:
2413 429 414 248 35
BanII G_rGCy'C
Cuts at: 365 529 553 1583 365
Size: 164 24 1030 2321
Fragments arranged by size:
2321 1030 164 24
BbeI G_GCGC'C
Cuts at: 973 1221 973
Size: 248 3291
BbsI GAAGACnn'nnnn_
Cuts at: 200 200
Size: 3539
BciVI GTATCCnnnnn_n'
Cuts at: 1437 2198 3539 1437
Size: 761 1341 1437
Fragments arranged by size:
1437 1341 761
BclI T'GATC_A
Cuts at: 110 1059 110
Size: 949 2590
BglII A'GATC_T
Cuts at: 1054 3091 1054
Size: 2037 1502
BlpI GC'TnA_GC
Cuts at: 410 651 410
Size: 241 3298
Bme1580I G_kGCm'C
Cuts at: 1164 1257 2313 1164
Size: 93 1056 2390
Fragments arranged by size:
2390 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 462 751 832 1167 3311 462
Size: 289 81 335 2144 690
Fragments arranged by size:
2144 690 335 289 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1906 1906
Size: 3539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 511
Size: 3539
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 364 715 1594 2086 2384 337
Size: 27 351 879 492 298 1492
Fragments arranged by size:
1492 879 492 351 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 3243 3243
Size: 3539
BsaAI yAC'GTr
Cuts at: 892 892
Size: 3539
BsaBI GATnn'nnATC
Cuts at: 6 1053 3090 6
Size: 1047 2037 455
Fragments arranged by size:
2037 1047 455
BsaHI Gr'CG_yC
Cuts at: 170 970 1218 1920 3416 170
Size: 800 248 702 1496 293
Fragments arranged by size:
1496 800 702 293 248
BsaWI w'CCGG_w
Cuts at: 728 1249 1927 2201 2348 2759 728
Size: 521 678 274 147 411 1508
Fragments arranged by size:
1508 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 406 457 406
Size: 51 3488
BseYI C'CCAG_C
Cuts at: 964 1722 2299 2795 964
Size: 758 577 496 1708
Fragments arranged by size:
1708 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1081 1127 2335 104
Size: 977 46 1208 1308
Fragments arranged by size:
1308 1208 977 46
BsiHKAI G_wGCw'C
Cuts at: 365 553 1331 1521 2313 365
Size: 188 778 190 792 1591
Fragments arranged by size:
1591 792 778 190 188
Bsp1286I G_dGCh'C
Cuts at: 365 529 553 1164 1257 1331 1521 1583 2313
Size: 164 24 611 93 74 190 62 730
Cuts at: 2313 365
Size: 1591
Fragments arranged by size:
1591 730 611 190 164 93 74 62
24
BspEI T'CCGG_A
Cuts at: 2759 2759
Size: 3539
BspHI T'CATG_A
Cuts at: 255 2715 255
Size: 2460 1079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 138 589 803 1105 1486 138
Size: 451 214 302 381 2191
Fragments arranged by size:
2191 451 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1831 1885 3369 3534 50
Size: 1781 54 1484 165 55
Fragments arranged by size:
1781 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 365 1451 365
Size: 1086 2453
BsrFI r'CCGG_y
Cuts at: 1537 1718 1927 1537
Size: 181 209 3149
Fragments arranged by size:
3149 209 181
BssSI C'ACGA_G
Cuts at: 1810 2168 1810
Size: 358 3181
BstAPI GCAn_nnn'nTGC
Cuts at: 916 916
Size: 3539
BstBI TT'CG_AA
Cuts at: 501 1900 501
Size: 1399 2140
BstXI CCAn_nnnn'nTGG
Cuts at: 207 207
Size: 3539
BstYI r'GATC_y
Cuts at: 1 564 1031 1054 1389 1635 2647 2733 2745
Size: 563 467 23 335 246 1012 86 12
Cuts at: 2745 3091 1
Size: 346 449
Fragments arranged by size:
1012 563 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 272 272
Size: 3539
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 135 1489 1670 1833 135
Size: 1354 181 163 1841
Fragments arranged by size:
1841 1354 181 163
BtsI GCAGTG_nn'
Cuts at: 291 782 291
Size: 491 3048
DraI TTT'AAA
Cuts at: 538 538
Size: 3539
DrdI GACnn_nn'nnGTC
Cuts at: 1245 2103 1245
Size: 858 2681
EaeI y'GGCC_r
Cuts at: 1124 1298 1689 1716 3061 1124
Size: 174 391 27 1345 1602
Fragments arranged by size:
1602 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1124 1124
Size: 3539
EarI CTCTTCn'nnn_
Cuts at: 383 511 1562 1772 383
Size: 128 1051 210 2150
Fragments arranged by size:
2150 1051 210 128
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1747 2057 2203 3349 1747
Size: 310 146 1146 1937
Fragments arranged by size:
1937 1146 310 146
EcoICRI GAG'CTC
Cuts at: 363 551 363
Size: 188 3351
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 511 1364 1796 2543 2811 212
Size: 299 853 432 747 268 940
Fragments arranged by size:
940 853 747 432 299 268
EcoO109I rG'GnC_Cy
Cuts at: 678 678
Size: 3539
EcoRI G'AATT_C
Cuts at: 424 543 424
Size: 119 3420
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 331 1558 331
Size: 1227 2312
FspI TGC'GCA
Cuts at: 1320 1320
Size: 3539
HaeII r_GCGC'y
Cuts at: 973 1221 2243 3222 973
Size: 248 1022 979 1290
Fragments arranged by size:
1290 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1381 1411 1413 1443 1381
Size: 30 2 30 3477
Fragments arranged by size:
3477 30 30 2
HincII GTy'rAC
Cuts at: 578 578
Size: 3539
KasI G'GCGC_C
Cuts at: 969 1217 969
Size: 248 3291
KpnI G_GTAC'C
Cuts at: 130 559 130
Size: 429 3110
MluI A'CGCG_T
Cuts at: 2972 2972
Size: 3539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2210 2394 2210
Size: 184 3355
MscI TGG'CCA
Cuts at: 1300 1300
Size: 3539
MslI CAynn'nnrTG
Cuts at: 277 1528 1655 277
Size: 1251 127 2161
Fragments arranged by size:
2161 1251 127
MspA1I CmG'CkG
Cuts at: 650 964 1324 2337 2582 650
Size: 314 360 1013 245 1607
Fragments arranged by size:
1607 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1720 1720
Size: 3539
NarI GG'CG_CC
Cuts at: 970 1218 970
Size: 248 3291
NcoI C'CATG_G
Cuts at: 272 272
Size: 3539
NgoMIV G'CCGG_C
Cuts at: 1718 1718
Size: 3539
NspI r_CATG'y
Cuts at: 592 1092 1999 592
Size: 500 907 2132
Fragments arranged by size:
2132 907 500
PciI A'CATG_T
Cuts at: 1995 1995
Size: 3539
PmeI GTTT'AAAC
Cuts at: 538 538
Size: 3539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2678 2710 2678
Size: 32 3507
PstI C_TGCA'G
Cuts at: 586 1271 586
Size: 685 2854
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3539
PvuII CAG'CTG
Cuts at: 964 1324 964
Size: 360 3179
RsrII CG'GwC_CG
Cuts at: 1734 1734
Size: 3539
SacI G_AGCT'C
Cuts at: 365 553 365
Size: 188 3351
SalI G'TCGA_C
Cuts at: 576 576
Size: 3539
SapI GCTCTTCn'nnn_
Cuts at: 1562 1772 1562
Size: 210 3329
SbfI CC_TGCA'GG
Cuts at: 586 586
Size: 3539
ScaI AGT'ACT
Cuts at: 3056 3056
Size: 3539
SfcI C'TryA_G
Cuts at: 33 235 582 1267 2260 2451 33
Size: 202 347 685 993 191 1121
Fragments arranged by size:
1121 993 685 347 202 191
SfoI GGC'GCC
Cuts at: 971 1219 971
Size: 248 3291
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3539
SmaI CCC'GGG
Cuts at: 561 561
Size: 3539
SmlI C'TyrA_G
Cuts at: 316 343 694 1609 2101 2363 316
Size: 27 351 915 492 262 1492
Fragments arranged by size:
1492 915 492 351 262 27
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3539
SphI G_CATG'C
Cuts at: 592 1092 592
Size: 500 3039
StyI C'CwwG_G
Cuts at: 272 673 1027 272
Size: 401 354 2784
Fragments arranged by size:
2784 401 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1122 1255 1714 1923 1122
Size: 133 459 209 2738
Fragments arranged by size:
2738 459 209 133
TatI w'GTAC_w
Cuts at: 3054 3054
Size: 3539
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3056 3088 3056
Size: 32 3507
Tth111I GACn'n_nGTC
Cuts at: 1336 1336
Size: 3539
XbaI T'CTAG_A
Cuts at: 570 570
Size: 3539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1034 1034
Size: 3539
XmaI C'CCGG_G
Cuts at: 559 559
Size: 3539
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI