Restriction map for
pF1KB8305
(Circular) (Six-base) MAPSORT of: pF1KB8305.seq Check: 4429 from: 1 to: 5084
pF1KB8305 5084 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AatII G_ACGT'C
Cuts at: 174 335 174
Size: 161 4923
AccI GT'mk_AC
Cuts at: 2122 2122
Size: 5084
Acc65I G'GTAC_C
Cuts at: 2100 2100
Size: 5084
AclI AA'CG_TT
Cuts at: 4924 4924
Size: 5084
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2018 2909 3341 4088 4356 2018
Size: 891 432 747 268 2746
Fragments arranged by size:
2746 891 747 432 268
AflIII A'CryG_T
Cuts at: 1040 1847 2436 3540 4517 1040
Size: 807 589 1104 977 1607
Fragments arranged by size:
1607 1104 977 807 589
AgeI A'CCGG_T
Cuts at: 3472 3472
Size: 5084
AleI CACnn'nnGTG
Cuts at: 1123 1870 1123
Size: 747 4337
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 782 1756 782
Size: 974 4110
AlwNI CAG_nnn'CTG
Cuts at: 1620 2389 3956 1620
Size: 769 1567 2748
Fragments arranged by size:
2748 1567 769
ApaLI G'TGCA_C
Cuts at: 1048 3854 1048
Size: 2806 2278
ApoI r'AATT_y
Cuts at: 319 864 2088 4437 4693 319
Size: 545 1224 2349 256 710
Fragments arranged by size:
2349 1224 710 545 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5084
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5084
AvaI C'yCGr_G
Cuts at: 673 2104 673
Size: 1431 3653
BamHI G'GATC_C
Cuts at: 843 2109 843
Size: 1266 3818
BanI G'GyrC_C
Cuts at: 689 1125 1199 1286 1312 2100 2514 2762 2797
Size: 436 74 87 26 788 414 248 35
Cuts at: 2797 689
Size: 2976
Fragments arranged by size:
2976 788 436 414 248 87 74 35
26
BanII G_rGCy'C
Cuts at: 470 680 1549 1807 2098 3128 470
Size: 210 869 258 291 1030 2426
Fragments arranged by size:
2426 1030 869 291 258 210
BbeI G_GCGC'C
Cuts at: 1203 1290 2518 2766 1203
Size: 87 1228 248 3521
Fragments arranged by size:
3521 1228 248 87
BbsI GAAGACnn'nnnn_
Cuts at: 1516 1516
Size: 5084
BbvCI CC'TCA_GC
Cuts at: 1958 2039 1958
Size: 81 5003
BciVI GTATCCnnnnn_n'
Cuts at: 2982 3743 5084 2982
Size: 761 1341 2982
Fragments arranged by size:
2982 1341 761
BclI T'GATC_A
Cuts at: 1814 2604 1814
Size: 790 4294
BglII A'GATC_T
Cuts at: 2599 4636 2599
Size: 2037 3047
BlpI GC'TnA_GC
Cuts at: 255 2196 255
Size: 1941 3143
Bme1580I G_kGCm'C
Cuts at: 945 1052 1130 1275 2709 2802 3858 945
Size: 107 78 145 1434 93 1056 2171
Fragments arranged by size:
2171 1434 1056 145 107 93 78
BmgBI CAC'GTC
Cuts at: 1344 1357 1848 1344
Size: 13 491 4580
Fragments arranged by size:
4580 491 13
BmrI ACTGGGnnnn_n'
Cuts at: 630 1262 2296 2377 2712 4856 630
Size: 632 1034 81 335 2144 858
Fragments arranged by size:
2144 1034 858 632 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3451 3451
Size: 5084
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 1116 1447 1642 142
Size: 974 331 195 3584
Fragments arranged by size:
3584 974 331 195
Bpu10I CC'TnA_GC
Cuts at: 749 850 1144 1958 2039 749
Size: 101 294 814 81 3794
Fragments arranged by size:
3794 814 294 101 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 516 2260 3139 3631 3929 516
Size: 1744 879 492 298 1671
Fragments arranged by size:
1744 1671 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 187 814 2022 4788 187
Size: 627 1208 2766 483
Fragments arranged by size:
2766 1208 627 483
BsaAI yAC'GTr
Cuts at: 1041 2437 1041
Size: 1396 3688
BsaBI GATnn'nnATC
Cuts at: 6 2598 4635 6
Size: 2592 2037 455
Fragments arranged by size:
2592 2037 455
BsaHI Gr'CG_yC
Cuts at: 171 332 1098 1200 1287 2515 2763 3465 4961
Size: 161 766 102 87 1228 248 702 1496
Cuts at: 4961 171
Size: 294
Fragments arranged by size:
1496 1228 766 702 294 248 161 102
87
BsaWI w'CCGG_w
Cuts at: 176 920 2273 2794 3472 3746 3893 4304 176
Size: 744 1353 521 678 274 147 411 956
Fragments arranged by size:
1353 956 744 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1248 1278 1615 1645 1248
Size: 30 337 30 4687
Fragments arranged by size:
4687 337 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2056 2056
Size: 5084
BseYI C'CCAG_C
Cuts at: 1699 1713 1943 2509 3267 3844 4340 1699
Size: 14 230 566 758 577 496 2443
Fragments arranged by size:
2443 758 577 566 496 230 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 878 1258 1495 1624 1848 317
Size: 561 380 237 129 224 3553
Fragments arranged by size:
3553 561 380 237 224 129
BsiEI CG_ry'CG
Cuts at: 104 2626 2672 3880 104
Size: 2522 46 1208 1308
Fragments arranged by size:
2522 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 353 413 470 1052 1150 1425 1581 2098 2876
Size: 60 57 582 98 275 156 517 778
Cuts at: 2876 3066 3858 353
Size: 190 792 1579
Fragments arranged by size:
1579 792 778 582 517 275 190 156
98 60 57
BsmI GAATG_Cn'
Cuts at: 1230 1230
Size: 5084
Bsp1286I G_dGCh'C
Cuts at: 353 413 470 680 945 1052 1130 1150 1275
Size: 60 57 210 265 107 78 20 125
Cuts at: 1275 1425 1549 1581 1807 2098 2709 2802 2876
Size: 150 124 32 226 291 611 93 74
Cuts at: 2876 3066 3128 3858 353
Size: 190 62 730 1579
Fragments arranged by size:
1579 730 611 291 265 226 210 190
150 125 124 107 93 78 74 62
60 57 32 20
BspEI T'CCGG_A
Cuts at: 920 4304 920
Size: 3384 1700
BspHI T'CATG_A
Cuts at: 137 4260 137
Size: 4123 961
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1365 1646 2134 2348 2650 3031 1365
Size: 281 488 214 302 381 3418
Fragments arranged by size:
3418 488 381 302 281 214
BsrBI CCG'CTC
Cuts at: 50 753 1139 3376 3430 4914 5079 50
Size: 703 386 2237 54 1484 165 55
Fragments arranged by size:
2237 1484 703 386 165 55 54
BsrDI GCAATG_nn'
Cuts at: 764 2996 764
Size: 2232 2852
BsrFI r'CCGG_y
Cuts at: 383 3082 3263 3472 383
Size: 2699 181 209 1995
Fragments arranged by size:
2699 1995 209 181
BssSI C'ACGA_G
Cuts at: 3355 3713 3355
Size: 358 4726
BstAPI GCAn_nnn'nTGC
Cuts at: 837 2461 837
Size: 1624 3460
BstBI TT'CG_AA
Cuts at: 3445 3445
Size: 5084
BstXI CCAn_nnnn'nTGG
Cuts at: 715 1527 715
Size: 812 4272
BstYI r'GATC_y
Cuts at: 1 843 1436 1616 2109 2576 2599 2934 3180
Size: 842 593 180 493 467 23 335 246
Cuts at: 3180 4192 4278 4290 4636 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 842 593 493 467 449 346 335
246 180 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 547 1808 1999 547
Size: 1261 191 3632
Fragments arranged by size:
3632 1261 191
BtgI C'CryG_G
Cuts at: 107 1194 1897 107
Size: 1087 703 3294
Fragments arranged by size:
3294 1087 703
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3034 3215 3378 3034
Size: 181 163 4740
Fragments arranged by size:
4740 181 163
BtsI GCAGTG_nn'
Cuts at: 838 861 2327 838
Size: 23 1466 3595
Fragments arranged by size:
3595 1466 23
DraI TTT'AAA
Cuts at: 2083 2083
Size: 5084
DraIII CAC_nnn'GTG
Cuts at: 417 417
Size: 5084
DrdI GACnn_nn'nnGTC
Cuts at: 1006 2790 3648 1006
Size: 1784 858 2442
Fragments arranged by size:
2442 1784 858
EaeI y'GGCC_r
Cuts at: 717 1919 1986 2669 2843 3234 3261 4606 717
Size: 1202 67 683 174 391 27 1345 1195
Fragments arranged by size:
1345 1202 1195 683 391 174 67 27
EagI C'GGCC_G
Cuts at: 2669 2669
Size: 5084
EarI CTCTTCn'nnn_
Cuts at: 3107 3317 3107
Size: 210 4874
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3292 3602 3748 4894 3292
Size: 310 146 1146 3482
Fragments arranged by size:
3482 1146 310 146
EcoICRI GAG'CTC
Cuts at: 468 2096 468
Size: 1628 3456
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 1116 1447 1642 2018 2909 3341 4088 4356
Size: 974 331 195 376 891 432 747 268
Cuts at: 4356 142
Size: 870
Fragments arranged by size:
974 891 870 747 432 376 331 268
195
EcoNI CCTnn'n_nnAGG
Cuts at: 894 1306 1320 894
Size: 412 14 4658
Fragments arranged by size:
4658 412 14
EcoO109I rG'GnC_Cy
Cuts at: 779 2223 779
Size: 1444 3640
EcoRI G'AATT_C
Cuts at: 2088 2088
Size: 5084
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 514 3103 514
Size: 2589 2495
FspI TGC'GCA
Cuts at: 2865 2865
Size: 5084
HaeII r_GCGC'y
Cuts at: 1035 1203 1290 2518 2766 3788 4767 1035
Size: 168 87 1228 248 1022 979 1352
Fragments arranged by size:
1352 1228 1022 979 248 168 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 514 546 898 930 1615 1647 1667 1699 2926
Size: 32 352 32 685 32 20 32 1227
Cuts at: 2926 2956 2958 2988 514
Size: 30 2 30 2610
Fragments arranged by size:
2610 1227 685 352 32 32 32 32
30 30 20 2
HincII GTy'rAC
Cuts at: 2123 2123
Size: 5084
KasI G'GCGC_C
Cuts at: 1199 1286 2514 2762 1199
Size: 87 1228 248 3521
Fragments arranged by size:
3521 1228 248 87
KpnI G_GTAC'C
Cuts at: 2104 2104
Size: 5084
MfeI C'AATT_G
Cuts at: 509 509
Size: 5084
MluI A'CGCG_T
Cuts at: 4517 4517
Size: 5084
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3755 3939 3755
Size: 184 4900
MscI TGG'CCA
Cuts at: 2845 2845
Size: 5084
MslI CAynn'nnrTG
Cuts at: 1123 1525 1870 2011 3073 3200 1123
Size: 402 345 141 1062 127 3007
Fragments arranged by size:
3007 1062 402 345 141 127
MspA1I CmG'CkG
Cuts at: 1820 1962 2038 2195 2509 2869 3882 4127 1820
Size: 142 76 157 314 360 1013 245 2777
Fragments arranged by size:
2777 1013 360 314 245 157 142 76
NaeI GCC'GGC
Cuts at: 385 3265 385
Size: 2880 2204
NarI GG'CG_CC
Cuts at: 1200 1287 2515 2763 1200
Size: 87 1228 248 3521
Fragments arranged by size:
3521 1228 248 87
NcoI C'CATG_G
Cuts at: 107 107
Size: 5084
NgoMIV G'CCGG_C
Cuts at: 383 3263 383
Size: 2880 2204
NspI r_CATG'y
Cuts at: 840 1539 1632 1773 2137 2637 3544 840
Size: 699 93 141 364 500 907 2380
Fragments arranged by size:
2380 907 699 500 364 141 93
PciI A'CATG_T
Cuts at: 3540 3540
Size: 5084
PflMI CCAn_nnn'nTGG
Cuts at: 891 1283 891
Size: 392 4692
PfoI T'CCnGG_A
Cuts at: 1619 1619
Size: 5084
PmeI GTTT'AAAC
Cuts at: 2083 2083
Size: 5084
PmlI CAC'GTG
Cuts at: 1041 1041
Size: 5084
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 793 825 4223 4255 793
Size: 32 3398 32 1622
Fragments arranged by size:
3398 1622 32 32
PpuMI rG'GwC_Cy
Cuts at: 779 779
Size: 5084
PshAI GACnn'nnGTC
Cuts at: 215 215
Size: 5084
PstI C_TGCA'G
Cuts at: 1745 2131 2816 1745
Size: 386 685 4013
Fragments arranged by size:
4013 685 386
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5084
PvuII CAG'CTG
Cuts at: 1962 2038 2509 2869 1962
Size: 76 471 360 4177
Fragments arranged by size:
4177 471 360 76
RsrII CG'GwC_CG
Cuts at: 3279 3279
Size: 5084
SacI G_AGCT'C
Cuts at: 470 2098 470
Size: 1628 3456
SalI G'TCGA_C
Cuts at: 2121 2121
Size: 5084
SapI GCTCTTCn'nnn_
Cuts at: 3107 3317 3107
Size: 210 4874
SbfI CC_TGCA'GG
Cuts at: 2131 2131
Size: 5084
ScaI AGT'ACT
Cuts at: 1480 4601 1480
Size: 3121 1963
SfcI C'TryA_G
Cuts at: 33 1062 1741 2127 2812 3805 3996 33
Size: 1029 679 386 685 993 191 1121
Fragments arranged by size:
1121 1029 993 685 679 386 191
SfoI GGC'GCC
Cuts at: 1201 1288 2516 2764 1201
Size: 87 1228 248 3521
Fragments arranged by size:
3521 1228 248 87
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5084
SmaI CCC'GGG
Cuts at: 2106 2106
Size: 5084
SmlI C'TyrA_G
Cuts at: 531 2239 3154 3646 3908 531
Size: 1708 915 492 262 1707
Fragments arranged by size:
1708 1707 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5084
SphI G_CATG'C
Cuts at: 840 1773 2137 2637 840
Size: 933 364 500 3287
Fragments arranged by size:
3287 933 500 364
StyI C'CwwG_G
Cuts at: 107 890 2218 2572 107
Size: 783 1328 354 2619
Fragments arranged by size:
2619 1328 783 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1957 2667 2800 3259 3468 1957
Size: 710 133 459 209 3573
Fragments arranged by size:
3573 710 459 209 133
TatI w'GTAC_w
Cuts at: 1478 1532 1604 4599 1478
Size: 54 72 2995 1963
Fragments arranged by size:
2995 1963 72 54
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4601 4633 4601
Size: 32 5052
Tth111I GACn'n_nGTC
Cuts at: 2881 2881
Size: 5084
XbaI T'CTAG_A
Cuts at: 2115 2115
Size: 5084
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2579 2579
Size: 5084
XmaI C'CCGG_G
Cuts at: 2104 2104
Size: 5084
ZraI GAC'GTC
Cuts at: 172 333 172
Size: 161 4923
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BglI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PasI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI