Restriction map for
pF1KB8304
(Circular) (Six-base) MAPSORT of: pF1KB8304.seq Check: 5449 from: 1 to: 4007
pF1KB8304 4007 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1045 1045
Size: 4007
Acc65I G'GTAC_C
Cuts at: 608 1023 608
Size: 415 3592
AclI AA'CG_TT
Cuts at: 3847 3847
Size: 4007
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1832 2264 3011 3279 1832
Size: 432 747 268 2560
Fragments arranged by size:
2560 747 432 268
AflII C'TTAA_G
Cuts at: 202 271 202
Size: 69 3938
AflIII A'CryG_T
Cuts at: 1359 2463 3440 1359
Size: 1104 977 1926
Fragments arranged by size:
1926 1104 977
AgeI A'CCGG_T
Cuts at: 2395 2395
Size: 4007
AleI CACnn'nnGTG
Cuts at: 655 655
Size: 4007
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 557 589 557
Size: 32 3975
AlwNI CAG_nnn'CTG
Cuts at: 504 1312 2879 504
Size: 808 1567 1632
Fragments arranged by size:
1632 1567 808
ApaI G_GGCC'C
Cuts at: 711 840 711
Size: 129 3878
ApaLI G'TGCA_C
Cuts at: 2777 2777
Size: 4007
ApoI r'AATT_y
Cuts at: 349 373 1011 3360 3616 349
Size: 24 638 2349 256 740
Fragments arranged by size:
2349 740 638 256 24
AseI AT'TA_AT
Cuts at: 20 20
Size: 4007
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4007
AvaI C'yCGr_G
Cuts at: 1027 1027
Size: 4007
BamHI G'GATC_C
Cuts at: 1032 1032
Size: 4007
BanI G'GyrC_C
Cuts at: 608 1023 1437 1685 1720 608
Size: 415 414 248 35 2895
Fragments arranged by size:
2895 415 414 248 35
BanII G_rGCy'C
Cuts at: 618 711 840 1021 2051 618
Size: 93 129 181 1030 2574
Fragments arranged by size:
2574 1030 181 129 93
BbeI G_GCGC'C
Cuts at: 1441 1689 1441
Size: 248 3759
BciVI GTATCCnnnnn_n'
Cuts at: 1905 2666 4007 1905
Size: 761 1341 1905
Fragments arranged by size:
1905 1341 761
BclI T'GATC_A
Cuts at: 1527 1527
Size: 4007
BglII A'GATC_T
Cuts at: 260 363 1522 3559 260
Size: 103 1159 2037 708
Fragments arranged by size:
2037 1159 708 103
BlpI GC'TnA_GC
Cuts at: 1119 1119
Size: 4007
Bme1580I G_kGCm'C
Cuts at: 148 242 711 840 1632 1725 2781 148
Size: 94 469 129 792 93 1056 1374
Fragments arranged by size:
1374 1056 792 469 129 94 93
BmrI ACTGGGnnnn_n'
Cuts at: 236 1219 1300 1635 3779 236
Size: 983 81 335 2144 464
Fragments arranged by size:
2144 983 464 335 81
BmtI G_CTAG'C
Cuts at: 538 538
Size: 4007
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 4007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 687 740 427
Size: 260 53 3694
Fragments arranged by size:
3694 260 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 1183 2062 2554 2852 352
Size: 831 879 492 298 1507
Fragments arranged by size:
1507 879 831 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 479 3711 479
Size: 3232 775
BsaAI yAC'GTr
Cuts at: 149 1360 149
Size: 1211 2796
BsaBI GATnn'nnATC
Cuts at: 6 1521 3558 6
Size: 1515 2037 455
Fragments arranged by size:
2037 1515 455
BsaHI Gr'CG_yC
Cuts at: 1438 1686 2388 3884 1438
Size: 248 702 1496 1561
Fragments arranged by size:
1561 1496 702 248
BsaWI w'CCGG_w
Cuts at: 746 1196 1717 2395 2669 2816 3227 746
Size: 450 521 678 274 147 411 1526
Fragments arranged by size:
1526 678 521 450 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 179 209 557 587 179
Size: 30 348 30 3599
Fragments arranged by size:
3599 348 30 30
BseYI C'CCAG_C
Cuts at: 1432 2190 2767 3263 1432
Size: 758 577 496 2176
Fragments arranged by size:
2176 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 892 340
Size: 552 3455
BsiEI CG_ry'CG
Cuts at: 104 1549 1595 2803 104
Size: 1445 46 1208 1308
Fragments arranged by size:
1445 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 618 1021 1799 1989 2781 618
Size: 403 778 190 792 1844
Fragments arranged by size:
1844 792 778 403 190
BsiWI C'GTAC_G
Cuts at: 149 149
Size: 4007
BsmI GAATG_Cn'
Cuts at: 401 401
Size: 4007
Bsp1286I G_dGCh'C
Cuts at: 148 242 618 711 840 1021 1632 1725 1799
Size: 94 376 93 129 181 611 93 74
Cuts at: 1799 1989 2051 2781 148
Size: 190 62 730 1374
Fragments arranged by size:
1374 730 611 376 190 181 129 94
93 93 74 62
BspEI T'CCGG_A
Cuts at: 746 3227 746
Size: 2481 1526
BspHI T'CATG_A
Cuts at: 3183 3183
Size: 4007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1057 1271 1573 1954 1057
Size: 214 302 381 3110
Fragments arranged by size:
3110 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2299 2353 3837 4002 50
Size: 2249 54 1484 165 55
Fragments arranged by size:
2249 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1919 1919
Size: 4007
BsrFI r'CCGG_y
Cuts at: 2005 2186 2395 2005
Size: 181 209 3617
Fragments arranged by size:
3617 209 181
BsrGI T'GTAC_A
Cuts at: 161 161
Size: 4007
BssSI C'ACGA_G
Cuts at: 2278 2636 2278
Size: 358 3649
BstAPI GCAn_nnn'nTGC
Cuts at: 906 1384 906
Size: 478 3529
BstBI TT'CG_AA
Cuts at: 2368 2368
Size: 4007
BstEII G'GTnAC_C
Cuts at: 597 597
Size: 4007
BstXI CCAn_nnnn'nTGG
Cuts at: 657 657
Size: 4007
BstYI r'GATC_y
Cuts at: 1 260 363 749 1032 1499 1522 1857 2103
Size: 259 103 386 283 467 23 335 246
Cuts at: 2103 3115 3201 3213 3559 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 386 346 335 283 259
246 103 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4007
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1957 2138 2301 1957
Size: 181 163 3663
Fragments arranged by size:
3663 181 163
BtsI GCAGTG_nn'
Cuts at: 1250 1250
Size: 4007
DraI TTT'AAA
Cuts at: 1006 1006
Size: 4007
DrdI GACnn_nn'nnGTC
Cuts at: 1713 2571 1713
Size: 858 3149
EaeI y'GGCC_r
Cuts at: 787 935 1592 1766 2157 2184 3529 787
Size: 148 657 174 391 27 1345 1265
Fragments arranged by size:
1345 1265 657 391 174 148 27
EagI C'GGCC_G
Cuts at: 1592 1592
Size: 4007
EarI CTCTTCn'nnn_
Cuts at: 413 748 2030 2240 413
Size: 335 1282 210 2180
Fragments arranged by size:
2180 1282 335 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 202 2215 2525 2671 3817 202
Size: 2013 310 146 1146 392
Fragments arranged by size:
2013 1146 392 310 146
EcoICRI GAG'CTC
Cuts at: 616 1019 616
Size: 403 3604
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 687 740 1832 2264 3011 3279 427
Size: 260 53 1092 432 747 268 1155
Fragments arranged by size:
1155 1092 747 432 268 260 53
EcoO109I rG'GnC_Cy
Cuts at: 447 474 708 767 1146 447
Size: 27 234 59 379 3308
Fragments arranged by size:
3308 379 234 59 27
EcoRI G'AATT_C
Cuts at: 1011 1011
Size: 4007
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2026 2026
Size: 4007
FspI TGC'GCA
Cuts at: 1788 1788
Size: 4007
HaeII r_GCGC'y
Cuts at: 202 1441 1689 2711 3690 202
Size: 1239 248 1022 979 519
Fragments arranged by size:
1239 1022 979 519 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 684 716 1849 1879 1881 1911 684
Size: 32 1133 30 2 30 2780
Fragments arranged by size:
2780 1133 32 30 30 2
HincII GTy'rAC
Cuts at: 182 1046 182
Size: 864 3143
KasI G'GCGC_C
Cuts at: 1437 1685 1437
Size: 248 3759
KpnI G_GTAC'C
Cuts at: 612 1027 612
Size: 415 3592
MluI A'CGCG_T
Cuts at: 3440 3440
Size: 4007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2678 2862 2678
Size: 184 3823
MscI TGG'CCA
Cuts at: 789 1768 789
Size: 979 3028
MslI CAynn'nnrTG
Cuts at: 655 1996 2123 655
Size: 1341 127 2539
Fragments arranged by size:
2539 1341 127
MspA1I CmG'CkG
Cuts at: 438 655 1118 1432 1792 2805 3050 438
Size: 217 463 314 360 1013 245 1395
Fragments arranged by size:
1395 1013 463 360 314 245 217
NaeI GCC'GGC
Cuts at: 2188 2188
Size: 4007
NarI GG'CG_CC
Cuts at: 1438 1686 1438
Size: 248 3759
NcoI C'CATG_G
Cuts at: 107 107
Size: 4007
NgoMIV G'CCGG_C
Cuts at: 2186 2186
Size: 4007
NheI G'CTAG_C
Cuts at: 534 534
Size: 4007
NspI r_CATG'y
Cuts at: 1060 1560 2467 1060
Size: 500 907 2600
Fragments arranged by size:
2600 907 500
PciI A'CATG_T
Cuts at: 2463 2463
Size: 4007
PflMI CCAn_nnn'nTGG
Cuts at: 126 594 126
Size: 468 3539
PfoI T'CCnGG_A
Cuts at: 965 965
Size: 4007
PmeI GTTT'AAAC
Cuts at: 1006 1006
Size: 4007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 557 589 3146 3178 557
Size: 32 2557 32 1386
Fragments arranged by size:
2557 1386 32 32
PpuMI rG'GwC_Cy
Cuts at: 474 767 474
Size: 293 3714
PspOMI G'GGCC_C
Cuts at: 707 836 707
Size: 129 3878
PstI C_TGCA'G
Cuts at: 1054 1739 1054
Size: 685 3322
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4007
PvuII CAG'CTG
Cuts at: 438 655 1432 1792 438
Size: 217 777 360 2653
Fragments arranged by size:
2653 777 360 217
RsrII CG'GwC_CG
Cuts at: 2202 2202
Size: 4007
SacI G_AGCT'C
Cuts at: 618 1021 618
Size: 403 3604
SalI G'TCGA_C
Cuts at: 1044 1044
Size: 4007
SanDI GG'GwC_CC
Cuts at: 767 767
Size: 4007
SapI GCTCTTCn'nnn_
Cuts at: 748 2030 2240 748
Size: 1282 210 2515
Fragments arranged by size:
2515 1282 210
SbfI CC_TGCA'GG
Cuts at: 1054 1054
Size: 4007
ScaI AGT'ACT
Cuts at: 246 3524 246
Size: 3278 729
SexAI A'CCwGG_T
Cuts at: 338 338
Size: 4007
SfcI C'TryA_G
Cuts at: 33 1050 1735 2728 2919 33
Size: 1017 685 993 191 1121
Fragments arranged by size:
1121 1017 993 685 191
SfoI GGC'GCC
Cuts at: 1439 1687 1439
Size: 248 3759
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4007
SmaI CCC'GGG
Cuts at: 1029 1029
Size: 4007
SmlI C'TyrA_G
Cuts at: 202 271 367 1162 2077 2569 2831 202
Size: 69 96 795 915 492 262 1378
Fragments arranged by size:
1378 915 795 492 262 96 69
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4007
SphI G_CATG'C
Cuts at: 1060 1560 1060
Size: 500 3507
SspI AAT'ATT
Cuts at: 294 643 294
Size: 349 3658
StyI C'CwwG_G
Cuts at: 107 938 1141 1495 107
Size: 831 203 354 2619
Fragments arranged by size:
2619 831 354 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 601 1590 1723 2182 2391 601
Size: 989 133 459 209 2217
Fragments arranged by size:
2217 989 459 209 133
TatI w'GTAC_w
Cuts at: 161 244 3522 161
Size: 83 3278 646
Fragments arranged by size:
3278 646 83
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 179 211 387 419 3524 3556 179
Size: 32 176 32 3105 32 630
Fragments arranged by size:
3105 630 176 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1804 1804
Size: 4007
XbaI T'CTAG_A
Cuts at: 1038 1038
Size: 4007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 945 1502 945
Size: 557 3450
XmaI C'CCGG_G
Cuts at: 1027 1027
Size: 4007
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmgBI Bpu10I BseRI
BsmBI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspXI
PsrI SacII SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI