Restriction map for
pF1KB8302
(Circular) (Six-base) MAPSORT of: pF1KB8302.seq Check: 3392 from: 1 to: 3788
pF1KB8302 3788 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 116 116
Size: 3788
AccI GT'mk_AC
Cuts at: 826 826
Size: 3788
Acc65I G'GTAC_C
Cuts at: 804 804
Size: 3788
AclI AA'CG_TT
Cuts at: 3628 3628
Size: 3788
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1613 2045 2792 3060 1613
Size: 432 747 268 2341
Fragments arranged by size:
2341 747 432 268
AflIII A'CryG_T
Cuts at: 617 654 1140 2244 3221 617
Size: 37 486 1104 977 1184
Fragments arranged by size:
1184 1104 977 486 37
AgeI A'CCGG_T
Cuts at: 2176 2176
Size: 3788
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 307 307
Size: 3788
AlwNI CAG_nnn'CTG
Cuts at: 300 1093 2660 300
Size: 793 1567 1428
Fragments arranged by size:
1567 1428 793
ApaLI G'TGCA_C
Cuts at: 2558 2558
Size: 3788
ApoI r'AATT_y
Cuts at: 211 274 715 792 3141 3397 211
Size: 63 441 77 2349 256 602
Fragments arranged by size:
2349 602 441 256 77 63
AseI AT'TA_AT
Cuts at: 20 20
Size: 3788
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
AvaI C'yCGr_G
Cuts at: 808 808
Size: 3788
BamHI G'GATC_C
Cuts at: 813 813
Size: 3788
BanI G'GyrC_C
Cuts at: 128 804 1218 1466 1501 128
Size: 676 414 248 35 2415
Fragments arranged by size:
2415 676 414 248 35
BanII G_rGCy'C
Cuts at: 139 802 1832 139
Size: 663 1030 2095
Fragments arranged by size:
2095 1030 663
BbeI G_GCGC'C
Cuts at: 1222 1470 1222
Size: 248 3540
BciVI GTATCCnnnnn_n'
Cuts at: 1686 2447 3788 1686
Size: 761 1341 1686
Fragments arranged by size:
1686 1341 761
BclI T'GATC_A
Cuts at: 1308 1308
Size: 3788
BglII A'GATC_T
Cuts at: 1303 3340 1303
Size: 2037 1751
BlpI GC'TnA_GC
Cuts at: 900 900
Size: 3788
Bme1580I G_kGCm'C
Cuts at: 1413 1506 2562 1413
Size: 93 1056 2639
Fragments arranged by size:
2639 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 188 366 1000 1081 1416 3560 188
Size: 178 634 81 335 2144 416
Fragments arranged by size:
2144 634 416 335 178 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 3788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 378 271
Size: 107 3681
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 964 1843 2335 2633 368
Size: 596 879 492 298 1523
Fragments arranged by size:
1523 879 596 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3492 3492
Size: 3788
BsaAI yAC'GTr
Cuts at: 620 657 1141 620
Size: 37 484 3267
Fragments arranged by size:
3267 484 37
BsaBI GATnn'nnATC
Cuts at: 6 1302 3339 6
Size: 1296 2037 455
Fragments arranged by size:
2037 1296 455
BsaHI Gr'CG_yC
Cuts at: 1219 1467 2169 3665 1219
Size: 248 702 1496 1342
Fragments arranged by size:
1496 1342 702 248
BsaWI w'CCGG_w
Cuts at: 977 1498 2176 2450 2597 3008 977
Size: 521 678 274 147 411 1757
Fragments arranged by size:
1757 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 361 652 361
Size: 291 3497
BseYI C'CCAG_C
Cuts at: 141 328 1213 1971 2548 3044 141
Size: 187 885 758 577 496 885
Fragments arranged by size:
885 885 758 577 496 187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 611
Size: 3788
BsiEI CG_ry'CG
Cuts at: 104 1330 1376 2584 104
Size: 1226 46 1208 1308
Fragments arranged by size:
1308 1226 1208 46
BsiHKAI G_wGCw'C
Cuts at: 802 1580 1770 2562 802
Size: 778 190 792 2028
Fragments arranged by size:
2028 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 139 802 1413 1506 1580 1770 1832 2562 139
Size: 663 611 93 74 190 62 730 1365
Fragments arranged by size:
1365 730 663 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3008 3008
Size: 3788
BspHI T'CATG_A
Cuts at: 584 749 2964 584
Size: 165 2215 1408
Fragments arranged by size:
2215 1408 165
BspMI ACCTGCnnnn'nnnn_
Cuts at: 116 285 838 1052 1354 1735 116
Size: 169 553 214 302 381 2169
Fragments arranged by size:
2169 553 381 302 214 169
BsrBI CCG'CTC
Cuts at: 50 2080 2134 3618 3783 50
Size: 2030 54 1484 165 55
Fragments arranged by size:
2030 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1700 1700
Size: 3788
BsrFI r'CCGG_y
Cuts at: 236 1786 1967 2176 236
Size: 1550 181 209 1848
Fragments arranged by size:
1848 1550 209 181
BssSI C'ACGA_G
Cuts at: 2059 2417 2059
Size: 358 3430
BstAPI GCAn_nnn'nTGC
Cuts at: 1165 1165
Size: 3788
BstBI TT'CG_AA
Cuts at: 2149 2149
Size: 3788
BstEII G'GTnAC_C
Cuts at: 702 702
Size: 3788
BstYI r'GATC_y
Cuts at: 1 813 1280 1303 1638 1884 2896 2982 2994
Size: 812 467 23 335 246 1012 86 12
Cuts at: 2994 3340 1
Size: 346 449
Fragments arranged by size:
1012 812 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 242 242
Size: 3788
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1738 1919 2082 1738
Size: 181 163 3444
Fragments arranged by size:
3444 181 163
BtsI GCAGTG_nn'
Cuts at: 1031 1031
Size: 3788
DraI TTT'AAA
Cuts at: 787 787
Size: 3788
DraIII CAC_nnn'GTG
Cuts at: 230 230
Size: 3788
DrdI GACnn_nn'nnGTC
Cuts at: 1494 2352 1494
Size: 858 2930
EaeI y'GGCC_r
Cuts at: 1373 1547 1938 1965 3310 1373
Size: 174 391 27 1345 1851
Fragments arranged by size:
1851 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1373 1373
Size: 3788
EarI CTCTTCn'nnn_
Cuts at: 421 1811 2021 421
Size: 1390 210 2188
Fragments arranged by size:
2188 1390 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1996 2306 2452 3598 1996
Size: 310 146 1146 2186
Fragments arranged by size:
2186 1146 310 146
EcoICRI GAG'CTC
Cuts at: 800 800
Size: 3788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 378 1613 2045 2792 3060 271
Size: 107 1235 432 747 268 999
Fragments arranged by size:
1235 999 747 432 268 107
EcoNI CCTnn'n_nnAGG
Cuts at: 544 544
Size: 3788
EcoO109I rG'GnC_Cy
Cuts at: 297 642 927 297
Size: 345 285 3158
Fragments arranged by size:
3158 345 285
EcoRI G'AATT_C
Cuts at: 715 792 715
Size: 77 3711
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1807 1807
Size: 3788
FspI TGC'GCA
Cuts at: 1569 1569
Size: 3788
HaeII r_GCGC'y
Cuts at: 1222 1470 2492 3471 1222
Size: 248 1022 979 1539
Fragments arranged by size:
1539 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1630 1660 1662 1692 1630
Size: 30 2 30 3726
Fragments arranged by size:
3726 30 30 2
HincII GTy'rAC
Cuts at: 827 827
Size: 3788
KasI G'GCGC_C
Cuts at: 1218 1466 1218
Size: 248 3540
KpnI G_GTAC'C
Cuts at: 808 808
Size: 3788
MluI A'CGCG_T
Cuts at: 3221 3221
Size: 3788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2459 2643 2459
Size: 184 3604
MscI TGG'CCA
Cuts at: 1549 1549
Size: 3788
MslI CAynn'nnrTG
Cuts at: 1777 1904 1777
Size: 127 3661
MspA1I CmG'CkG
Cuts at: 141 171 258 332 899 1213 1573 2586 2831
Size: 30 87 74 567 314 360 1013 245
Cuts at: 2831 141
Size: 1098
Fragments arranged by size:
1098 1013 567 360 314 245 87 74
30
NaeI GCC'GGC
Cuts at: 1969 1969
Size: 3788
NarI GG'CG_CC
Cuts at: 1219 1467 1219
Size: 248 3540
NgoMIV G'CCGG_C
Cuts at: 1967 1967
Size: 3788
NspI r_CATG'y
Cuts at: 841 1341 2248 841
Size: 500 907 2381
Fragments arranged by size:
2381 907 500
PciI A'CATG_T
Cuts at: 2244 2244
Size: 3788
PflMI CCAn_nnn'nTGG
Cuts at: 678 678
Size: 3788
PfoI T'CCnGG_A
Cuts at: 632 632
Size: 3788
PmeI GTTT'AAAC
Cuts at: 787 787
Size: 3788
PmlI CAC'GTG
Cuts at: 657 657
Size: 3788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2927 2959 2927
Size: 32 3756
PpuMI rG'GwC_Cy
Cuts at: 297 297
Size: 3788
PstI C_TGCA'G
Cuts at: 263 835 1520 263
Size: 572 685 2531
Fragments arranged by size:
2531 685 572
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3788
PvuII CAG'CTG
Cuts at: 171 258 1213 1573 171
Size: 87 955 360 2386
Fragments arranged by size:
2386 955 360 87
RsrII CG'GwC_CG
Cuts at: 1983 1983
Size: 3788
SacI G_AGCT'C
Cuts at: 802 802
Size: 3788
SalI G'TCGA_C
Cuts at: 825 825
Size: 3788
SapI GCTCTTCn'nnn_
Cuts at: 1811 2021 1811
Size: 210 3578
SbfI CC_TGCA'GG
Cuts at: 835 835
Size: 3788
ScaI AGT'ACT
Cuts at: 3305 3305
Size: 3788
SfcI C'TryA_G
Cuts at: 33 259 500 831 1516 2509 2700 33
Size: 226 241 331 685 993 191 1121
Fragments arranged by size:
1121 993 685 331 241 226 191
SfoI GGC'GCC
Cuts at: 1220 1468 1220
Size: 248 3540
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
SmaI CCC'GGG
Cuts at: 810 810
Size: 3788
SmlI C'TyrA_G
Cuts at: 383 943 1858 2350 2612 383
Size: 560 915 492 262 1559
Fragments arranged by size:
1559 915 560 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3788
SphI G_CATG'C
Cuts at: 841 1341 841
Size: 500 3288
StyI C'CwwG_G
Cuts at: 563 646 922 1276 563
Size: 83 276 354 3075
Fragments arranged by size:
3075 354 276 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 438 1371 1504 1963 2172 438
Size: 933 133 459 209 2054
Fragments arranged by size:
2054 933 459 209 133
TatI w'GTAC_w
Cuts at: 614 3303 614
Size: 2689 1099
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 3756
Tth111I GACn'n_nGTC
Cuts at: 180 775 1585 180
Size: 595 810 2383
Fragments arranged by size:
2383 810 595
XbaI T'CTAG_A
Cuts at: 819 819
Size: 3788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 175 1283 175
Size: 1108 2680
XmaI C'CCGG_G
Cuts at: 808 808
Size: 3788
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstXI BstZ17I BtgI ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI