Restriction map for
pF1KB8301
(Circular) (Six-base) MAPSORT of: pF1KB8301.seq Check: 6957 from: 1 to: 4547
pF1KB8301 4547 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 681 681
Size: 4547
AccI GT'mk_AC
Cuts at: 479 1454 1461 1585 479
Size: 975 7 124 3441
Fragments arranged by size:
3441 975 124 7
Acc65I G'GTAC_C
Cuts at: 1563 1563
Size: 4547
AclI AA'CG_TT
Cuts at: 4387 4387
Size: 4547
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2372 2804 3551 3819 2372
Size: 432 747 268 3100
Fragments arranged by size:
3100 747 432 268
AflIII A'CryG_T
Cuts at: 656 1899 3003 3980 656
Size: 1243 1104 977 1223
Fragments arranged by size:
1243 1223 1104 977
AgeI A'CCGG_T
Cuts at: 2935 2935
Size: 4547
AhdI GACnn_n'nnGTC
Cuts at: 1468 1468
Size: 4547
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 526 526
Size: 4547
AlwNI CAG_nnn'CTG
Cuts at: 118 747 848 1533 1852 3419 118
Size: 629 101 685 319 1567 1246
Fragments arranged by size:
1567 1246 685 629 319 101
ApaLI G'TGCA_C
Cuts at: 3317 3317
Size: 4547
ApoI r'AATT_y
Cuts at: 1551 3900 4156 1551
Size: 2349 256 1942
Fragments arranged by size:
2349 1942 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4547
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
AvaI C'yCGr_G
Cuts at: 572 1176 1567 572
Size: 604 391 3552
Fragments arranged by size:
3552 604 391
BamHI G'GATC_C
Cuts at: 1572 1572
Size: 4547
BanI G'GyrC_C
Cuts at: 1563 1977 2225 2260 1563
Size: 414 248 35 3850
Fragments arranged by size:
3850 414 248 35
BanII G_rGCy'C
Cuts at: 343 611 1161 1561 2591 343
Size: 268 550 400 1030 2299
Fragments arranged by size:
2299 1030 550 400 268
BbeI G_GCGC'C
Cuts at: 1981 2229 1981
Size: 248 4299
BbsI GAAGACnn'nnnn_
Cuts at: 376 776 376
Size: 400 4147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1175 1209 1175
Size: 34 4513
BciVI GTATCCnnnnn_n'
Cuts at: 2445 3206 4547 2445
Size: 761 1341 2445
Fragments arranged by size:
2445 1341 761
BclI T'GATC_A
Cuts at: 2067 2067
Size: 4547
BglI GCCn_nnn'nGGC
Cuts at: 238 238
Size: 4547
BglII A'GATC_T
Cuts at: 2062 4099 2062
Size: 2037 2510
BlpI GC'TnA_GC
Cuts at: 1123 1659 1123
Size: 536 4011
Bme1580I G_kGCm'C
Cuts at: 276 728 1389 2172 2265 3321 276
Size: 452 661 783 93 1056 1502
Fragments arranged by size:
1502 1056 783 661 452 93
BmrI ACTGGGnnnn_n'
Cuts at: 858 1095 1376 1759 1840 2175 4319 858
Size: 237 281 383 81 335 2144 1086
Fragments arranged by size:
2144 1086 383 335 281 237 81
BmtI G_CTAG'C
Cuts at: 1099 1099
Size: 4547
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2914 2914
Size: 4547
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 581 757 1021 1391 581
Size: 176 264 370 3737
Fragments arranged by size:
3737 370 264 176
Bpu10I CC'TnA_GC
Cuts at: 295 379 295
Size: 84 4463
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 684 1183 1192 1362 1723 2602 3094 3392 684
Size: 499 9 170 361 879 492 298 1839
Fragments arranged by size:
1839 879 499 492 361 298 170 9
BsaI GGTCTCn'nnnn_
Cuts at: 1310 4251 1310
Size: 2941 1606
BsaAI yAC'GTr
Cuts at: 1900 1900
Size: 4547
BsaBI GATnn'nnATC
Cuts at: 6 2061 4098 6
Size: 2055 2037 455
Fragments arranged by size:
2055 2037 455
BsaHI Gr'CG_yC
Cuts at: 1978 2226 2928 4424 1978
Size: 248 702 1496 2101
Fragments arranged by size:
2101 1496 702 248
BsaWI w'CCGG_w
Cuts at: 536 611 1308 1736 2257 2935 3209 3356 3767
Size: 75 697 428 521 678 274 147 411
Cuts at: 3767 536
Size: 1316
Fragments arranged by size:
1316 697 678 521 428 411 274 147
75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 994 1024 994
Size: 30 4517
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 402 1306 402
Size: 904 3643
BseYI C'CCAG_C
Cuts at: 198 222 317 1972 2730 3307 3803 198
Size: 24 95 1655 758 577 496 942
Fragments arranged by size:
1655 942 758 577 496 95 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1027
Size: 4547
BsiEI CG_ry'CG
Cuts at: 104 261 1326 2089 2135 3343 104
Size: 157 1065 763 46 1208 1308
Fragments arranged by size:
1308 1208 1065 763 157 46
BsiHKAI G_wGCw'C
Cuts at: 179 611 1561 2339 2529 3321 179
Size: 432 950 778 190 792 1405
Fragments arranged by size:
1405 950 792 778 432 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1135 1135
Size: 4547
Bsp1286I G_dGCh'C
Cuts at: 179 276 343 611 728 1161 1389 1561 2172
Size: 97 67 268 117 433 228 172 611
Cuts at: 2172 2265 2339 2529 2591 3321 179
Size: 93 74 190 62 730 1405
Fragments arranged by size:
1405 730 611 433 268 228 190 172
117 97 93 74 67 62
BspEI T'CCGG_A
Cuts at: 611 1308 3767 611
Size: 697 2459 1391
Fragments arranged by size:
2459 1391 697
BspHI T'CATG_A
Cuts at: 779 3723 779
Size: 2944 1603
BspMI ACCTGCnnnn'nnnn_
Cuts at: 681 695 1597 1811 2113 2494 681
Size: 14 902 214 302 381 2734
Fragments arranged by size:
2734 902 381 302 214 14
BsrBI CCG'CTC
Cuts at: 50 214 2839 2893 4377 4542 50
Size: 164 2625 54 1484 165 55
Fragments arranged by size:
2625 1484 165 164 55 54
BsrDI GCAATG_nn'
Cuts at: 2459 2459
Size: 4547
BsrFI r'CCGG_y
Cuts at: 383 1091 2545 2726 2935 383
Size: 708 1454 181 209 1995
Fragments arranged by size:
1995 1454 708 209 181
BssSI C'ACGA_G
Cuts at: 833 2818 3176 833
Size: 1985 358 2204
Fragments arranged by size:
2204 1985 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1924 1924
Size: 4547
BstBI TT'CG_AA
Cuts at: 2908 2908
Size: 4547
BstXI CCAn_nnnn'nTGG
Cuts at: 336 459 336
Size: 123 4424
BstYI r'GATC_y
Cuts at: 1 1202 1572 2039 2062 2397 2643 3655 3741
Size: 1201 370 467 23 335 246 1012 86
Cuts at: 3741 3753 4099 1
Size: 12 346 449
Fragments arranged by size:
1201 1012 467 449 370 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1136 107
Size: 1029 3518
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 213 2497 2678 2841 213
Size: 2284 181 163 1919
Fragments arranged by size:
2284 1919 181 163
BtsI GCAGTG_nn'
Cuts at: 1247 1790 1247
Size: 543 4004
DraI TTT'AAA
Cuts at: 1546 1546
Size: 4547
DrdI GACnn_nn'nnGTC
Cuts at: 2253 3111 2253
Size: 858 3689
EaeI y'GGCC_r
Cuts at: 161 215 461 934 1079 1319 1507 2132 2306
Size: 54 246 473 145 240 188 625 174
Cuts at: 2306 2697 2724 4069 161
Size: 391 27 1345 639
Fragments arranged by size:
1345 639 625 473 391 246 240 188
174 145 54 27
EagI C'GGCC_G
Cuts at: 2132 2132
Size: 4547
EarI CTCTTCn'nnn_
Cuts at: 1036 2570 2780 1036
Size: 1534 210 2803
Fragments arranged by size:
2803 1534 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 958 2755 3065 3211 4357 958
Size: 1797 310 146 1146 1148
Fragments arranged by size:
1797 1148 1146 310 146
EcoICRI GAG'CTC
Cuts at: 609 1559 609
Size: 950 3597
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 581 757 1021 1391 2372 2804 3551 3819 581
Size: 176 264 370 981 432 747 268 1309
Fragments arranged by size:
1309 981 747 432 370 268 264 176
EcoO109I rG'GnC_Cy
Cuts at: 947 1530 1686 947
Size: 583 156 3808
Fragments arranged by size:
3808 583 156
EcoRI G'AATT_C
Cuts at: 1551 1551
Size: 4547
EcoRV GAT'ATC
Cuts at: 1432 1432
Size: 4547
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 4547
FspI TGC'GCA
Cuts at: 2328 2328
Size: 4547
HaeII r_GCGC'y
Cuts at: 119 1981 2229 3251 4230 119
Size: 1862 248 1022 979 436
Fragments arranged by size:
1862 1022 979 436 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 243 275 1116 1148 1454 1486 2389 2419 2421
Size: 32 841 32 306 32 903 30 2
Cuts at: 2421 2451 243
Size: 30 2339
Fragments arranged by size:
2339 903 841 306 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1462 1586 1462
Size: 124 4423
KasI G'GCGC_C
Cuts at: 1977 2225 1977
Size: 248 4299
KpnI G_GTAC'C
Cuts at: 1567 1567
Size: 4547
MfeI C'AATT_G
Cuts at: 930 930
Size: 4547
MluI A'CGCG_T
Cuts at: 3980 3980
Size: 4547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 738 3218 3402 738
Size: 2480 184 1883
Fragments arranged by size:
2480 1883 184
MscI TGG'CCA
Cuts at: 463 936 1081 1509 2308 463
Size: 473 145 428 799 2702
Fragments arranged by size:
2702 799 473 428 145
MslI CAynn'nnrTG
Cuts at: 778 2536 2663 778
Size: 1758 127 2662
Fragments arranged by size:
2662 1758 127
MspA1I CmG'CkG
Cuts at: 703 857 1138 1475 1658 1972 2332 3345 3590
Size: 154 281 337 183 314 360 1013 245
Cuts at: 3590 703
Size: 1660
Fragments arranged by size:
1660 1013 360 337 314 281 245 183
154
NaeI GCC'GGC
Cuts at: 2728 2728
Size: 4547
NarI GG'CG_CC
Cuts at: 1978 2226 1978
Size: 248 4299
NcoI C'CATG_G
Cuts at: 107 107
Size: 4547
NdeI CA'TA_TG
Cuts at: 598 1216 598
Size: 618 3929
NgoMIV G'CCGG_C
Cuts at: 2726 2726
Size: 4547
NheI G'CTAG_C
Cuts at: 1095 1095
Size: 4547
NspI r_CATG'y
Cuts at: 507 645 660 1600 2100 3007 507
Size: 138 15 940 500 907 2047
Fragments arranged by size:
2047 940 907 500 138 15
PciI A'CATG_T
Cuts at: 656 3003 656
Size: 2347 2200
PflMI CCAn_nnn'nTGG
Cuts at: 932 932
Size: 4547
PmeI GTTT'AAAC
Cuts at: 1546 1546
Size: 4547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3718 3686
Size: 32 4515
PpuMI rG'GwC_Cy
Cuts at: 947 947
Size: 4547
PstI C_TGCA'G
Cuts at: 515 692 1337 1594 2279 515
Size: 177 645 257 685 2783
Fragments arranged by size:
2783 685 645 257 177
PvuI CG_AT'CG
Cuts at: 104 261 104
Size: 157 4390
PvuII CAG'CTG
Cuts at: 703 1475 1972 2332 703
Size: 772 497 360 2918
Fragments arranged by size:
2918 772 497 360
RsrII CG'GwC_CG
Cuts at: 2742 2742
Size: 4547
SacI G_AGCT'C
Cuts at: 611 1561 611
Size: 950 3597
SacII CC_GC'GG
Cuts at: 1139 1139
Size: 4547
SalI G'TCGA_C
Cuts at: 1460 1584 1460
Size: 124 4423
SanDI GG'GwC_CC
Cuts at: 947 947
Size: 4547
SapI GCTCTTCn'nnn_
Cuts at: 2570 2780 2570
Size: 210 4337
SbfI CC_TGCA'GG
Cuts at: 515 692 1337 1594 515
Size: 177 645 257 3468
Fragments arranged by size:
3468 645 257 177
ScaI AGT'ACT
Cuts at: 826 4064 826
Size: 3238 1309
SfcI C'TryA_G
Cuts at: 33 511 688 1333 1590 2275 3268 3459 33
Size: 478 177 645 257 685 993 191 1121
Fragments arranged by size:
1121 993 685 645 478 257 191 177
SfoI GGC'GCC
Cuts at: 1979 2227 1979
Size: 248 4299
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
SmaI CCC'GGG
Cuts at: 1569 1569
Size: 4547
SmlI C'TyrA_G
Cuts at: 663 1198 1207 1341 1702 2617 3109 3371 663
Size: 535 9 134 361 915 492 262 1839
Fragments arranged by size:
1839 915 535 492 361 262 134 9
SpeI A'CTAG_T
Cuts at: 67 1491 67
Size: 1424 3123
SphI G_CATG'C
Cuts at: 1600 2100 1600
Size: 500 4047
StuI AGG'CCT
Cuts at: 1339 1408 1339
Size: 69 4478
StyI C'CwwG_G
Cuts at: 107 1397 1681 2035 107
Size: 1290 284 354 2619
Fragments arranged by size:
2619 1290 354 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 196 2130 2263 2722 2931 196
Size: 1934 133 459 209 1812
Fragments arranged by size:
1934 1812 459 209 133
TatI w'GTAC_w
Cuts at: 659 824 4062 659
Size: 165 3238 1144
Fragments arranged by size:
3238 1144 165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4064 4096 4064
Size: 32 4515
Tth111I GACn'n_nGTC
Cuts at: 1069 2344 1069
Size: 1275 3272
XbaI T'CTAG_A
Cuts at: 1578 1578
Size: 4547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 189 1233 1492 2042 189
Size: 1044 259 550 2694
Fragments arranged by size:
2694 1044 550 259
XmaI C'CCGG_G
Cuts at: 1567 1567
Size: 4547
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NspI PciI PflMI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BmgBI BsiWI BsmI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI