Restriction map for
pF1KB8300
(Circular) (Six-base) MAPSORT of: pF1KB8300.seq Check: 6226 from: 1 to: 4502
pF1KB8300 4502 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1362 1362
Size: 4502
AatII G_ACGT'C
Cuts at: 854 1300 854
Size: 446 4056
AccI GT'mk_AC
Cuts at: 1394 1540 1394
Size: 146 4356
Acc65I G'GTAC_C
Cuts at: 1518 1518
Size: 4502
AclI AA'CG_TT
Cuts at: 4342 4342
Size: 4502
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2327 2759 3506 3774 2327
Size: 432 747 268 3055
Fragments arranged by size:
3055 747 432 268
AflIII A'CryG_T
Cuts at: 1854 2958 3935 1854
Size: 1104 977 2421
Fragments arranged by size:
2421 1104 977
AgeI A'CCGG_T
Cuts at: 2890 2890
Size: 4502
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 953 953
Size: 4502
AlwNI CAG_nnn'CTG
Cuts at: 387 1807 3374 387
Size: 1420 1567 1515
Fragments arranged by size:
1567 1515 1420
ApaI G_GGCC'C
Cuts at: 394 1053 394
Size: 659 3843
ApaLI G'TGCA_C
Cuts at: 823 3272 823
Size: 2449 2053
ApoI r'AATT_y
Cuts at: 1506 3855 4111 1506
Size: 2349 256 1897
Fragments arranged by size:
2349 1897 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4502
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4502
AvaI C'yCGr_G
Cuts at: 1522 1522
Size: 4502
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1352 1385 1352
Size: 33 4469
BamHI G'GATC_C
Cuts at: 1527 1527
Size: 4502
BanI G'GyrC_C
Cuts at: 175 631 1068 1117 1518 1932 2180 2215 175
Size: 456 437 49 401 414 248 35 2462
Fragments arranged by size:
2462 456 437 414 401 248 49 35
BanII G_rGCy'C
Cuts at: 394 574 766 803 1053 1135 1516 2546 394
Size: 180 192 37 250 82 381 1030 2350
Fragments arranged by size:
2350 1030 381 250 192 180 82 37
BbeI G_GCGC'C
Cuts at: 1072 1936 2184 1072
Size: 864 248 3390
Fragments arranged by size:
3390 864 248
BbsI GAAGACnn'nnnn_
Cuts at: 361 361
Size: 4502
BciVI GTATCCnnnnn_n'
Cuts at: 2400 3161 4502 2400
Size: 761 1341 2400
Fragments arranged by size:
2400 1341 761
BclI T'GATC_A
Cuts at: 683 1079 2022 683
Size: 396 943 3163
Fragments arranged by size:
3163 943 396
BglI GCCn_nnn'nGGC
Cuts at: 319 344 946 1139 319
Size: 25 602 193 3682
Fragments arranged by size:
3682 602 193 25
BglII A'GATC_T
Cuts at: 710 2017 4054 710
Size: 1307 2037 1158
Fragments arranged by size:
2037 1307 1158
BlpI GC'TnA_GC
Cuts at: 1614 1614
Size: 4502
Bme1580I G_kGCm'C
Cuts at: 180 349 394 634 790 827 1053 1120 2127
Size: 169 45 240 156 37 226 67 1007
Cuts at: 2127 2220 3276 180
Size: 93 1056 1406
Fragments arranged by size:
1406 1056 1007 240 226 169 156 93
67 45 37
BmgBI CAC'GTC
Cuts at: 681 681
Size: 4502
BmrI ACTGGGnnnn_n'
Cuts at: 208 1714 1795 2130 4274 208
Size: 1506 81 335 2144 436
Fragments arranged by size:
2144 1506 436 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1050 1437 2869 1050
Size: 387 1432 2683
Fragments arranged by size:
2683 1432 387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 460 787 1007 1075 1312 307
Size: 153 327 220 68 237 3497
Fragments arranged by size:
3497 327 237 220 153 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 1678 2557 3049 3347 580
Size: 1098 879 492 298 1735
Fragments arranged by size:
1735 1098 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 607 1474 4206 607
Size: 867 2732 903
Fragments arranged by size:
2732 903 867
BsaAI yAC'GTr
Cuts at: 1855 1855
Size: 4502
BsaBI GATnn'nnATC
Cuts at: 6 688 2016 4053 6
Size: 682 1328 2037 455
Fragments arranged by size:
2037 1328 682 455
BsaHI Gr'CG_yC
Cuts at: 851 1069 1262 1297 1451 1933 2181 2883 4379
Size: 218 193 35 154 482 248 702 1496
Cuts at: 4379 851
Size: 974
Fragments arranged by size:
1496 974 702 482 248 218 193 154
35
BsaWI w'CCGG_w
Cuts at: 1226 1691 2212 2890 3164 3311 3722 1226
Size: 465 521 678 274 147 411 2006
Fragments arranged by size:
2006 678 521 465 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 898 928 898
Size: 30 4472
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 520 976 520
Size: 456 4046
BseYI C'CCAG_C
Cuts at: 1927 2685 3262 3758 1927
Size: 758 577 496 2671
Fragments arranged by size:
2671 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 802 823 1390 802
Size: 21 567 3914
Fragments arranged by size:
3914 567 21
BsiEI CG_ry'CG
Cuts at: 104 318 2044 2090 3298 104
Size: 214 1726 46 1208 1308
Fragments arranged by size:
1726 1308 1208 214 46
BsiHKAI G_wGCw'C
Cuts at: 803 827 1516 2294 2484 3276 803
Size: 24 689 778 190 792 2029
Fragments arranged by size:
2029 792 778 689 190 24
BsmBI CGTCTCn'nnnn_
Cuts at: 502 1182 1288 502
Size: 680 106 3716
Fragments arranged by size:
3716 680 106
Bsp1286I G_dGCh'C
Cuts at: 180 349 394 574 634 766 790 803 827
Size: 169 45 180 60 132 24 13 24
Cuts at: 827 1053 1120 1135 1516 2127 2220 2294 2484
Size: 226 67 15 381 611 93 74 190
Cuts at: 2484 2546 3276 180
Size: 62 730 1406
Fragments arranged by size:
1406 730 611 381 226 190 180 169
132 93 74 67 62 60 45 24
24 15 13
BspEI T'CCGG_A
Cuts at: 3722 3722
Size: 4502
BspHI T'CATG_A
Cuts at: 3678 3678
Size: 4502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1163 1362 1552 1766 2068 2449 1163
Size: 199 190 214 302 381 3216
Fragments arranged by size:
3216 381 302 214 199 190
BsrBI CCG'CTC
Cuts at: 50 728 1421 2794 2848 4332 4497 50
Size: 678 693 1373 54 1484 165 55
Fragments arranged by size:
1484 1373 693 678 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2414 2414
Size: 4502
BsrFI r'CCGG_y
Cuts at: 313 418 1163 2500 2681 2890 313
Size: 105 745 1337 181 209 1925
Fragments arranged by size:
1925 1337 745 209 181 105
BssSI C'ACGA_G
Cuts at: 841 2773 3131 841
Size: 1932 358 2212
Fragments arranged by size:
2212 1932 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1229 1879 1229
Size: 650 3852
BstBI TT'CG_AA
Cuts at: 2863 2863
Size: 4502
BstXI CCAn_nnnn'nTGG
Cuts at: 369 375 609 369
Size: 6 234 4262
Fragments arranged by size:
4262 234 6
BstYI r'GATC_y
Cuts at: 1 143 539 710 1214 1527 1994 2017 2352
Size: 142 396 171 504 313 467 23 335
Cuts at: 2352 2598 3610 3696 3708 4054 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 504 467 449 396 346 335 313
246 171 142 86 23 12
BtgI C'CryG_G
Cuts at: 107 368 1095 1484 107
Size: 261 727 389 3125
Fragments arranged by size:
3125 727 389 261
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 348 399 2452 2633 2796 348
Size: 51 2053 181 163 2054
Fragments arranged by size:
2054 2053 181 163 51
BtsI GCAGTG_nn'
Cuts at: 429 790 1300 1745 429
Size: 361 510 445 3186
Fragments arranged by size:
3186 510 445 361
DraI TTT'AAA
Cuts at: 1501 1501
Size: 4502
DraIII CAC_nnn'GTG
Cuts at: 831 831
Size: 4502
DrdI GACnn_nn'nnGTC
Cuts at: 2208 3066 2208
Size: 858 3644
EaeI y'GGCC_r
Cuts at: 336 359 1085 2087 2261 2652 2679 4024 336
Size: 23 726 1002 174 391 27 1345 814
Fragments arranged by size:
1345 1002 814 726 391 174 27 23
EagI C'GGCC_G
Cuts at: 2087 2087
Size: 4502
EarI CTCTTCn'nnn_
Cuts at: 892 1408 1465 2525 2735 892
Size: 516 57 1060 210 2659
Fragments arranged by size:
2659 1060 516 210 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 988 2710 3020 3166 4312 988
Size: 1722 310 146 1146 1178
Fragments arranged by size:
1722 1178 1146 310 146
EcoICRI GAG'CTC
Cuts at: 801 1514 801
Size: 713 3789
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 460 787 1007 1075 1312 2327 2759 3506
Size: 153 327 220 68 237 1015 432 747
Cuts at: 3506 3774 307
Size: 268 1035
Fragments arranged by size:
1035 1015 747 432 327 268 237 220
153 68
EcoNI CCTnn'n_nnAGG
Cuts at: 655 655
Size: 4502
EcoO109I rG'GnC_Cy
Cuts at: 384 391 1050 1641 384
Size: 7 659 591 3245
Fragments arranged by size:
3245 659 591 7
EcoRI G'AATT_C
Cuts at: 1506 1506
Size: 4502
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2521 2521
Size: 4502
FspI TGC'GCA
Cuts at: 2283 2283
Size: 4502
HaeII r_GCGC'y
Cuts at: 463 886 1072 1936 2184 3206 4185 463
Size: 423 186 864 248 1022 979 780
Fragments arranged by size:
1022 979 864 780 423 248 186
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 300 332 898 930 2344 2374 2376 2406 300
Size: 32 566 32 1414 30 2 30 2396
Fragments arranged by size:
2396 1414 566 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1541 1541
Size: 4502
KasI G'GCGC_C
Cuts at: 1068 1932 2180 1068
Size: 864 248 3390
Fragments arranged by size:
3390 864 248
KpnI G_GTAC'C
Cuts at: 1522 1522
Size: 4502
MluI A'CGCG_T
Cuts at: 3935 3935
Size: 4502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3173 3357 3173
Size: 184 4318
MscI TGG'CCA
Cuts at: 338 1087 2263 338
Size: 749 1176 2577
Fragments arranged by size:
2577 1176 749
MslI CAynn'nnrTG
Cuts at: 367 373 607 637 2491 2618 367
Size: 6 234 30 1854 127 2251
Fragments arranged by size:
2251 1854 234 127 30 6
MspA1I CmG'CkG
Cuts at: 255 1330 1613 1927 2287 3300 3545 255
Size: 1075 283 314 360 1013 245 1212
Fragments arranged by size:
1212 1075 1013 360 314 283 245
NaeI GCC'GGC
Cuts at: 420 2683 420
Size: 2263 2239
NarI GG'CG_CC
Cuts at: 1069 1933 2181 1069
Size: 864 248 3390
Fragments arranged by size:
3390 864 248
NcoI C'CATG_G
Cuts at: 107 368 1484 107
Size: 261 1116 3125
Fragments arranged by size:
3125 1116 261
NgoMIV G'CCGG_C
Cuts at: 418 2681 418
Size: 2263 2239
NspI r_CATG'y
Cuts at: 1017 1555 2055 2962 1017
Size: 538 500 907 2557
Fragments arranged by size:
2557 907 538 500
PasI CC'CwG_GG
Cuts at: 387 870 387
Size: 483 4019
PciI A'CATG_T
Cuts at: 2958 2958
Size: 4502
PflMI CCAn_nnn'nTGG
Cuts at: 1017 1017
Size: 4502
PfoI T'CCnGG_A
Cuts at: 137 296 1406 137
Size: 159 1110 3233
Fragments arranged by size:
3233 1110 159
PmeI GTTT'AAAC
Cuts at: 1501 1501
Size: 4502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3641 3673 3641
Size: 32 4470
PspOMI G'GGCC_C
Cuts at: 390 1049 390
Size: 659 3843
PstI C_TGCA'G
Cuts at: 1549 2234 1549
Size: 685 3817
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4502
PvuII CAG'CTG
Cuts at: 255 1330 1927 2287 255
Size: 1075 597 360 2470
Fragments arranged by size:
2470 1075 597 360
RsrII CG'GwC_CG
Cuts at: 2697 2697
Size: 4502
SacI G_AGCT'C
Cuts at: 803 1516 803
Size: 713 3789
SalI G'TCGA_C
Cuts at: 1539 1539
Size: 4502
SapI GCTCTTCn'nnn_
Cuts at: 892 2525 2735 892
Size: 1633 210 2659
Fragments arranged by size:
2659 1633 210
SbfI CC_TGCA'GG
Cuts at: 1549 1549
Size: 4502
ScaI AGT'ACT
Cuts at: 4019 4019
Size: 4502
SfcI C'TryA_G
Cuts at: 33 1191 1545 2230 3223 3414 33
Size: 1158 354 685 993 191 1121
Fragments arranged by size:
1158 1121 993 685 354 191
SfoI GGC'GCC
Cuts at: 1070 1934 2182 1070
Size: 864 248 3390
Fragments arranged by size:
3390 864 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4502
SmaI CCC'GGG
Cuts at: 1524 1524
Size: 4502
SmlI C'TyrA_G
Cuts at: 595 1657 2572 3064 3326 595
Size: 1062 915 492 262 1771
Fragments arranged by size:
1771 1062 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4502
SphI G_CATG'C
Cuts at: 1555 2055 1555
Size: 500 4002
StuI AGG'CCT
Cuts at: 472 472
Size: 4502
StyI C'CwwG_G
Cuts at: 107 215 368 575 656 941 1484 1636 1990
Size: 108 153 207 81 285 543 152 354
Cuts at: 1990 107
Size: 2619
Fragments arranged by size:
2619 543 354 285 207 153 152 108
81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 469 883 2085 2218 2677 2886 469
Size: 414 1202 133 459 209 2085
Fragments arranged by size:
2085 1202 459 414 209 133
TatI w'GTAC_w
Cuts at: 1430 4017 1430
Size: 2587 1915
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 817 849 4019 4051 817
Size: 32 3170 32 1268
Fragments arranged by size:
3170 1268 32 32
Tth111I GACn'n_nGTC
Cuts at: 664 1006 2299 664
Size: 342 1293 2867
Fragments arranged by size:
2867 1293 342
XbaI T'CTAG_A
Cuts at: 1533 1533
Size: 4502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 624 1155 1997 624
Size: 531 842 3129
Fragments arranged by size:
3129 842 531
XmaI C'CCGG_G
Cuts at: 1522 1522
Size: 4502
ZraI GAC'GTC
Cuts at: 852 1298 852
Size: 446 4056
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI AscI AvrII BbvCI
BcgI BfrBI BmtI Bpu10I BsiWI BsmI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI