Restriction map for
pF1KB8299
(Circular) (Six-base) MAPSORT of: pF1KB8299.seq Check: 183 from: 1 to: 4724
pF1KB8299 4724 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1762 1762
Size: 4724
Acc65I G'GTAC_C
Cuts at: 1740 1740
Size: 4724
AclI AA'CG_TT
Cuts at: 4564 4564
Size: 4724
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 740 1108 1183 2549 2981 3728 3996 740
Size: 368 75 1366 432 747 268 1468
Fragments arranged by size:
1468 1366 747 432 368 268 75
AflIII A'CryG_T
Cuts at: 2076 3180 4157 2076
Size: 1104 977 2643
Fragments arranged by size:
2643 1104 977
AgeI A'CCGG_T
Cuts at: 3112 3112
Size: 4724
AleI CACnn'nnGTG
Cuts at: 637 1285 637
Size: 648 4076
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 402 496 655 402
Size: 94 159 4471
Fragments arranged by size:
4471 159 94
AlwNI CAG_nnn'CTG
Cuts at: 372 507 651 663 1194 2029 3596 372
Size: 135 144 12 531 835 1567 1500
Fragments arranged by size:
1567 1500 835 531 144 135 12
ApaLI G'TGCA_C
Cuts at: 3494 3494
Size: 4724
ApoI r'AATT_y
Cuts at: 1728 4077 4333 1728
Size: 2349 256 2119
Fragments arranged by size:
2349 2119 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4724
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4724
AvaI C'yCGr_G
Cuts at: 116 329 1417 1634 1744 116
Size: 213 1088 217 110 3096
Fragments arranged by size:
3096 1088 217 213 110
BamHI G'GATC_C
Cuts at: 1749 1749
Size: 4724
BanI G'GyrC_C
Cuts at: 1740 2154 2402 2437 1740
Size: 414 248 35 4027
Fragments arranged by size:
4027 414 248 35
BanII G_rGCy'C
Cuts at: 116 1738 2768 116
Size: 1622 1030 2072
Fragments arranged by size:
2072 1622 1030
BbeI G_GCGC'C
Cuts at: 2158 2406 2158
Size: 248 4476
BbsI GAAGACnn'nnnn_
Cuts at: 842 842
Size: 4724
BciVI GTATCCnnnnn_n'
Cuts at: 2622 3383 4724 2622
Size: 761 1341 2622
Fragments arranged by size:
2622 1341 761
BclI T'GATC_A
Cuts at: 2244 2244
Size: 4724
BglII A'GATC_T
Cuts at: 2239 4276 2239
Size: 2037 2687
BlpI GC'TnA_GC
Cuts at: 484 1111 1836 484
Size: 627 725 3372
Fragments arranged by size:
3372 725 627
Bme1580I G_kGCm'C
Cuts at: 1044 1134 1340 2349 2442 3498 1044
Size: 90 206 1009 93 1056 2270
Fragments arranged by size:
2270 1056 1009 206 93 90
BmrI ACTGGGnnnn_n'
Cuts at: 1936 2017 2352 4496 1936
Size: 81 335 2144 2164
Fragments arranged by size:
2164 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1118 3091 1118
Size: 1973 2751
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 715 920 1006 1581 472
Size: 243 205 86 575 3615
Fragments arranged by size:
3615 575 243 205 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 943 1900 2779 3271 3569 445
Size: 498 957 879 492 298 1600
Fragments arranged by size:
1600 957 879 498 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 264 896 4428 264
Size: 632 3532 560
Fragments arranged by size:
3532 632 560
BsaAI yAC'GTr
Cuts at: 2077 2077
Size: 4724
BsaBI GATnn'nnATC
Cuts at: 6 2238 4275 6
Size: 2232 2037 455
Fragments arranged by size:
2232 2037 455
BsaHI Gr'CG_yC
Cuts at: 2155 2403 3105 4601 2155
Size: 248 702 1496 2278
Fragments arranged by size:
2278 1496 702 248
BsaWI w'CCGG_w
Cuts at: 233 1913 2434 3112 3386 3533 3944 233
Size: 1680 521 678 274 147 411 1013
Fragments arranged by size:
1680 1013 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 725 755 1579 1609 725
Size: 30 824 30 3840
Fragments arranged by size:
3840 824 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 582 598 928 967 1036 1522 1558 1683
Size: 419 16 330 39 69 486 36 125
Cuts at: 1683 163
Size: 3204
Fragments arranged by size:
3204 486 419 330 125 69 39 36
16
BseYI C'CCAG_C
Cuts at: 705 1146 2149 2907 3484 3980 705
Size: 441 1003 758 577 496 1449
Fragments arranged by size:
1449 1003 758 577 496 441
BsiEI CG_ry'CG
Cuts at: 104 2266 2312 3520 104
Size: 2162 46 1208 1308
Fragments arranged by size:
2162 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 194 485 791 1327 1559 1738 2516 2706 3498
Size: 291 306 536 232 179 778 190 792
Cuts at: 3498 194
Size: 1420
Fragments arranged by size:
1420 792 778 536 306 291 232 190
179
BsmI GAATG_Cn'
Cuts at: 1374 1374
Size: 4724
BsmBI CGTCTCn'nnnn_
Cuts at: 133 133
Size: 4724
Bsp1286I G_dGCh'C
Cuts at: 116 194 485 791 1044 1134 1327 1340 1559
Size: 78 291 306 253 90 193 13 219
Cuts at: 1559 1738 2349 2442 2516 2706 2768 3498 116
Size: 179 611 93 74 190 62 730 1342
Fragments arranged by size:
1342 730 611 306 291 253 219 193
190 179 93 90 78 74 62 13
BspEI T'CCGG_A
Cuts at: 3944 3944
Size: 4724
BspHI T'CATG_A
Cuts at: 1265 3900 1265
Size: 2635 2089
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1774 1988 2290 2671 1774
Size: 214 302 381 3827
Fragments arranged by size:
3827 381 302 214
BsrBI CCG'CTC
Cuts at: 50 765 3016 3070 4554 4719 50
Size: 715 2251 54 1484 165 55
Fragments arranged by size:
2251 1484 715 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2636 2636
Size: 4724
BsrFI r'CCGG_y
Cuts at: 1412 2722 2903 3112 1412
Size: 1310 181 209 3024
Fragments arranged by size:
3024 1310 209 181
BssSI C'ACGA_G
Cuts at: 478 1558 2995 3353 478
Size: 1080 1437 358 1849
Fragments arranged by size:
1849 1437 1080 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2101 2101
Size: 4724
BstBI TT'CG_AA
Cuts at: 3085 3085
Size: 4724
BstEII G'GTnAC_C
Cuts at: 1575 1575
Size: 4724
BstXI CCAn_nnnn'nTGG
Cuts at: 1572 1572
Size: 4724
BstYI r'GATC_y
Cuts at: 1 776 1749 2216 2239 2574 2820 3832 3918
Size: 775 973 467 23 335 246 1012 86
Cuts at: 3918 3930 4276 1
Size: 12 346 449
Fragments arranged by size:
1012 973 775 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1226 1226
Size: 4724
BtgI C'CryG_G
Cuts at: 107 134 770 921 1004 107
Size: 27 636 151 83 3827
Fragments arranged by size:
3827 636 151 83 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2674 2855 3018 2674
Size: 181 163 4380
Fragments arranged by size:
4380 181 163
BtsI GCAGTG_nn'
Cuts at: 938 1967 938
Size: 1029 3695
DraI TTT'AAA
Cuts at: 1723 1723
Size: 4724
DraIII CAC_nnn'GTG
Cuts at: 882 933 882
Size: 51 4673
DrdI GACnn_nn'nnGTC
Cuts at: 403 2430 3288 403
Size: 2027 858 1839
Fragments arranged by size:
2027 1839 858
EaeI y'GGCC_r
Cuts at: 924 1177 2309 2483 2874 2901 4246 924
Size: 253 1132 174 391 27 1345 1402
Fragments arranged by size:
1402 1345 1132 391 253 174 27
EagI C'GGCC_G
Cuts at: 2309 2309
Size: 4724
EarI CTCTTCn'nnn_
Cuts at: 232 616 796 1285 2747 2957 232
Size: 384 180 489 1462 210 1999
Fragments arranged by size:
1999 1462 489 384 210 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 262 1249 2932 3242 3388 4534 262
Size: 987 1683 310 146 1146 452
Fragments arranged by size:
1683 1146 987 452 310 146
EcoICRI GAG'CTC
Cuts at: 1736 1736
Size: 4724
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 715 740 920 1006 1108 1183 1581 2549
Size: 243 25 180 86 102 75 398 968
Cuts at: 2549 2981 3728 3996 472
Size: 432 747 268 1200
Fragments arranged by size:
1200 968 747 432 398 268 243 180
102 86 75 25
EcoNI CCTnn'n_nnAGG
Cuts at: 271 271
Size: 4724
EcoO109I rG'GnC_Cy
Cuts at: 863 1395 1863 863
Size: 532 468 3724
Fragments arranged by size:
3724 532 468
EcoRI G'AATT_C
Cuts at: 1728 1728
Size: 4724
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2743 2743
Size: 4724
FseI GG_CCGG'CC
Cuts at: 1416 1416
Size: 4724
FspI TGC'GCA
Cuts at: 2505 2505
Size: 4724
HaeII r_GCGC'y
Cuts at: 2158 2406 3428 4407 2158
Size: 248 1022 979 2475
Fragments arranged by size:
2475 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1579 1611 1690 1722 2566 2596 2598 2628 1579
Size: 32 79 32 844 30 2 30 3675
Fragments arranged by size:
3675 844 79 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1763 1763
Size: 4724
HindIII A'AGCT_T
Cuts at: 1252 1252
Size: 4724
KasI G'GCGC_C
Cuts at: 2154 2402 2154
Size: 248 4476
KpnI G_GTAC'C
Cuts at: 1744 1744
Size: 4724
MluI A'CGCG_T
Cuts at: 4157 4157
Size: 4724
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3395 3579 3395
Size: 184 4540
MscI TGG'CCA
Cuts at: 1179 2485 1179
Size: 1306 3418
MslI CAynn'nnrTG
Cuts at: 637 1018 1285 2713 2840 637
Size: 381 267 1428 127 2521
Fragments arranged by size:
2521 1428 381 267 127
MspA1I CmG'CkG
Cuts at: 651 1835 2149 2509 3522 3767 651
Size: 1184 314 360 1013 245 1608
Fragments arranged by size:
1608 1184 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1414 2905 1414
Size: 1491 3233
NarI GG'CG_CC
Cuts at: 2155 2403 2155
Size: 248 4476
NcoI C'CATG_G
Cuts at: 107 770 107
Size: 663 4061
NgoMIV G'CCGG_C
Cuts at: 1412 2903 1412
Size: 1491 3233
NspI r_CATG'y
Cuts at: 1777 2277 3184 1777
Size: 500 907 3317
Fragments arranged by size:
3317 907 500
PasI CC'CwG_GG
Cuts at: 1170 1170
Size: 4724
PciI A'CATG_T
Cuts at: 3180 3180
Size: 4724
PflMI CCAn_nnn'nTGG
Cuts at: 1620 1620
Size: 4724
PfoI T'CCnGG_A
Cuts at: 266 1597 266
Size: 1331 3393
PmeI GTTT'AAAC
Cuts at: 1723 1723
Size: 4724
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3863 3895 3863
Size: 32 4692
PpuMI rG'GwC_Cy
Cuts at: 863 863
Size: 4724
PstI C_TGCA'G
Cuts at: 311 377 1658 1771 2456 311
Size: 66 1281 113 685 2579
Fragments arranged by size:
2579 1281 685 113 66
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4724
PvuII CAG'CTG
Cuts at: 651 2149 2509 651
Size: 1498 360 2866
Fragments arranged by size:
2866 1498 360
RsrII CG'GwC_CG
Cuts at: 2919 2919
Size: 4724
SacI G_AGCT'C
Cuts at: 1738 1738
Size: 4724
SalI G'TCGA_C
Cuts at: 1761 1761
Size: 4724
SanDI GG'GwC_CC
Cuts at: 863 863
Size: 4724
SapI GCTCTTCn'nnn_
Cuts at: 796 2747 2957 796
Size: 1951 210 2563
Fragments arranged by size:
2563 1951 210
SbfI CC_TGCA'GG
Cuts at: 377 1658 1771 377
Size: 1281 113 3330
Fragments arranged by size:
3330 1281 113
ScaI AGT'ACT
Cuts at: 1429 4241 1429
Size: 2812 1912
SfcI C'TryA_G
Cuts at: 33 307 373 1654 1767 2452 3445 3636 33
Size: 274 66 1281 113 685 993 191 1121
Fragments arranged by size:
1281 1121 993 685 274 191 113 66
SfoI GGC'GCC
Cuts at: 2156 2404 2156
Size: 248 4476
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4724
SmaI CCC'GGG
Cuts at: 118 331 1636 1746 118
Size: 213 1305 110 3096
Fragments arranged by size:
3096 1305 213 110
SmlI C'TyrA_G
Cuts at: 460 958 1879 2794 3286 3548 460
Size: 498 921 915 492 262 1636
Fragments arranged by size:
1636 921 915 498 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4724
SphI G_CATG'C
Cuts at: 1777 2277 1777
Size: 500 4224
StyI C'CwwG_G
Cuts at: 107 770 1858 2212 107
Size: 663 1088 354 2619
Fragments arranged by size:
2619 1088 663 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 750 2307 2440 2899 3108 750
Size: 1557 133 459 209 2366
Fragments arranged by size:
2366 1557 459 209 133
TatI w'GTAC_w
Cuts at: 447 1352 1427 1460 4239 447
Size: 905 75 33 2779 932
Fragments arranged by size:
2779 932 905 75 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 725 729 757 761 1651 1683 4241 4273 725
Size: 4 28 4 890 32 2558 32 1176
Fragments arranged by size:
2558 1176 890 32 32 28 4 4
Tth111I GACn'n_nGTC
Cuts at: 2521 2521
Size: 4724
XbaI T'CTAG_A
Cuts at: 1755 1755
Size: 4724
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1011 1398 2219 1011
Size: 387 821 3516
Fragments arranged by size:
3516 821 387
XmaI C'CCGG_G
Cuts at: 116 329 1634 1744 116
Size: 213 1305 110 3096
Fragments arranged by size:
3096 1305 213 110
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsrGI BssHII BstZ17I ClaI CspCI
EcoRV FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI