Restriction map for
pF1KB8295
(Circular) (Six-base) MAPSORT of: pF1KB8295.seq Check: 3381 from: 1 to: 4532
pF1KB8295 4532 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 156 156
Size: 4532
AccI GT'mk_AC
Cuts at: 1570 1570
Size: 4532
Acc65I G'GTAC_C
Cuts at: 263 1548 263
Size: 1285 3247
AclI AA'CG_TT
Cuts at: 4372 4372
Size: 4532
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 234 467 773 1281 2357 2789 3536 3804 234
Size: 233 306 508 1076 432 747 268 962
Fragments arranged by size:
1076 962 747 508 432 306 268 233
AfeI AGC'GCT
Cuts at: 247 444 247
Size: 197 4335
AflIII A'CryG_T
Cuts at: 1022 1884 2988 3965 1022
Size: 862 1104 977 1589
Fragments arranged by size:
1589 1104 977 862
AgeI A'CCGG_T
Cuts at: 534 1524 2920 534
Size: 990 1396 2146
Fragments arranged by size:
2146 1396 990
AhdI GACnn_n'nnGTC
Cuts at: 505 505
Size: 4532
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 655 687 655
Size: 32 4500
AlwNI CAG_nnn'CTG
Cuts at: 360 387 565 1837 3404 360
Size: 27 178 1272 1567 1488
Fragments arranged by size:
1567 1488 1272 178 27
ApaI G_GGCC'C
Cuts at: 148 638 148
Size: 490 4042
ApaLI G'TGCA_C
Cuts at: 1026 3302 1026
Size: 2276 2256
ApoI r'AATT_y
Cuts at: 1536 3885 4141 1536
Size: 2349 256 1927
Fragments arranged by size:
2349 1927 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4532
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4532
AvaI C'yCGr_G
Cuts at: 284 334 579 1552 284
Size: 50 245 973 3264
Fragments arranged by size:
3264 973 245 50
AvrII C'CTAG_G
Cuts at: 148 148
Size: 4532
BamHI G'GATC_C
Cuts at: 1247 1557 1247
Size: 310 4222
BanI G'GyrC_C
Cuts at: 263 1179 1548 1962 2210 2245 263
Size: 916 369 414 248 35 2550
Fragments arranged by size:
2550 916 414 369 248 35
BanII G_rGCy'C
Cuts at: 148 410 638 767 1141 1546 2576 148
Size: 262 228 129 374 405 1030 2104
Fragments arranged by size:
2104 1030 405 374 262 228 129
BbeI G_GCGC'C
Cuts at: 1183 1966 2214 1183
Size: 783 248 3501
Fragments arranged by size:
3501 783 248
BbvCI CC'TCA_GC
Cuts at: 328 1315 328
Size: 987 3545
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 570 604 570
Size: 34 4498
BciVI GTATCCnnnnn_n'
Cuts at: 2430 3191 4532 2430
Size: 761 1341 2430
Fragments arranged by size:
2430 1341 761
BclI T'GATC_A
Cuts at: 2052 2052
Size: 4532
BglII A'GATC_T
Cuts at: 471 2047 4084 471
Size: 1576 2037 919
Fragments arranged by size:
2037 1576 919
BlpI GC'TnA_GC
Cuts at: 1644 1644
Size: 4532
Bme1580I G_kGCm'C
Cuts at: 148 638 1030 2157 2250 3306 148
Size: 490 392 1127 93 1056 1374
Fragments arranged by size:
1374 1127 1056 490 392 93
BmrI ACTGGGnnnn_n'
Cuts at: 130 1016 1265 1744 1825 2160 4304 130
Size: 886 249 479 81 335 2144 358
Fragments arranged by size:
2144 886 479 358 335 249 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 897 2899 897
Size: 2002 2530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 382 394 397 496 1529 250
Size: 132 12 3 99 1033 3253
Fragments arranged by size:
3253 1033 132 99 12 3
Bpu10I CC'TnA_GC
Cuts at: 295 328 1315 295
Size: 33 987 3512
Fragments arranged by size:
3512 987 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 662 1708 2587 3079 3377 662
Size: 1046 879 492 298 1817
Fragments arranged by size:
1817 1046 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 513 601 4236 513
Size: 88 3635 809
Fragments arranged by size:
3635 809 88
BsaAI yAC'GTr
Cuts at: 1023 1885 1023
Size: 862 3670
BsaBI GATnn'nnATC
Cuts at: 6 2046 4083 6
Size: 2040 2037 455
Fragments arranged by size:
2040 2037 455
BsaHI Gr'CG_yC
Cuts at: 1180 1963 2211 2913 4409 1180
Size: 783 248 702 1496 1303
Fragments arranged by size:
1496 1303 783 702 248
BsaWI w'CCGG_w
Cuts at: 534 1339 1524 1721 2242 2920 3194 3341 3752
Size: 805 185 197 521 678 274 147 411
Cuts at: 3752 534
Size: 1314
Fragments arranged by size:
1314 805 678 521 411 274 197 185
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 655 685 1375 1405 655
Size: 30 690 30 3782
Fragments arranged by size:
3782 690 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 208 454 506 546 676 208
Size: 246 52 40 130 4064
Fragments arranged by size:
4064 246 130 52 40
BseYI C'CCAG_C
Cuts at: 716 1957 2715 3292 3788 716
Size: 1241 758 577 496 1460
Fragments arranged by size:
1460 1241 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 442
Size: 4532
BsiEI CG_ry'CG
Cuts at: 104 2074 2120 3328 104
Size: 1970 46 1208 1308
Fragments arranged by size:
1970 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 314 410 1030 1141 1546 2324 2514 3306 314
Size: 96 620 111 405 778 190 792 1540
Fragments arranged by size:
1540 792 778 620 405 190 111 96
BsmI GAATG_Cn'
Cuts at: 1169 1169
Size: 4532
BsmBI CGTCTCn'nnnn_
Cuts at: 924 924
Size: 4532
Bsp1286I G_dGCh'C
Cuts at: 148 314 410 638 767 1030 1141 1546 2157
Size: 166 96 228 129 263 111 405 611
Cuts at: 2157 2250 2324 2514 2576 3306 148
Size: 93 74 190 62 730 1374
Fragments arranged by size:
1374 730 611 405 263 228 190 166
129 111 96 93 74 62
BspEI T'CCGG_A
Cuts at: 3752 3752
Size: 4532
BspHI T'CATG_A
Cuts at: 3708 3708
Size: 4532
BspMI ACCTGCnnnn'nnnn_
Cuts at: 156 1582 1796 2098 2479 156
Size: 1426 214 302 381 2209
Fragments arranged by size:
2209 1426 381 302 214
BsrBI CCG'CTC
Cuts at: 50 480 2824 2878 4362 4527 50
Size: 430 2344 54 1484 165 55
Fragments arranged by size:
2344 1484 430 165 55 54
BsrDI GCAATG_nn'
Cuts at: 429 2444 429
Size: 2015 2517
BsrFI r'CCGG_y
Cuts at: 534 1097 1524 2530 2711 2920 534
Size: 563 427 1006 181 209 2146
Fragments arranged by size:
2146 1006 563 427 209 181
BsrGI T'GTAC_A
Cuts at: 641 641
Size: 4532
BssSI C'ACGA_G
Cuts at: 721 2803 3161 721
Size: 2082 358 2092
Fragments arranged by size:
2092 2082 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1909 1909
Size: 4532
BstBI TT'CG_AA
Cuts at: 2893 2893
Size: 4532
BstXI CCAn_nnnn'nTGG
Cuts at: 655 655
Size: 4532
BstYI r'GATC_y
Cuts at: 1 338 356 471 681 1247 1557 2024 2047
Size: 337 18 115 210 566 310 467 23
Cuts at: 2047 2382 2628 3640 3726 3738 4084 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 566 467 449 346 337 335 310
246 210 115 86 23 18 12
Bsu36I CC'TnA_GG
Cuts at: 558 806 558
Size: 248 4284
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2482 2663 2826 2482
Size: 181 163 4188
Fragments arranged by size:
4188 181 163
BtsI GCAGTG_nn'
Cuts at: 832 1775 832
Size: 943 3589
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 584 619 1182 1217 584
Size: 35 563 35 3899
Fragments arranged by size:
3899 563 35 35
DraI TTT'AAA
Cuts at: 1531 1531
Size: 4532
DraIII CAC_nnn'GTG
Cuts at: 1034 1092 1034
Size: 58 4474
DrdI GACnn_nn'nnGTC
Cuts at: 2238 3096 2238
Size: 858 3674
EaeI y'GGCC_r
Cuts at: 2117 2291 2682 2709 4054 2117
Size: 174 391 27 1345 2595
Fragments arranged by size:
2595 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2117 2117
Size: 4532
EarI CTCTTCn'nnn_
Cuts at: 197 443 872 1169 1285 2555 2765 197
Size: 246 429 297 116 1270 210 1964
Fragments arranged by size:
1964 1270 429 297 246 210 116
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 229 2740 3050 3196 4342 229
Size: 2511 310 146 1146 419
Fragments arranged by size:
2511 1146 419 310 146
EcoICRI GAG'CTC
Cuts at: 408 1139 1544 408
Size: 731 405 3396
Fragments arranged by size:
3396 731 405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 234 250 382 394 397 467 496 773 1281
Size: 16 132 12 3 70 29 277 508
Cuts at: 1281 1529 2357 2789 3536 3804 234
Size: 248 828 432 747 268 962
Fragments arranged by size:
962 828 747 508 432 277 268 248
132 70 29 16 12 3
EcoNI CCTnn'n_nnAGG
Cuts at: 400 920 400
Size: 520 4012
EcoO109I rG'GnC_Cy
Cuts at: 145 172 291 634 635 1671 145
Size: 27 119 343 1 1036 3006
Fragments arranged by size:
3006 1036 343 119 27 1
EcoRI G'AATT_C
Cuts at: 1536 1536
Size: 4532
EcoRV GAT'ATC
Cuts at: 551 551
Size: 4532
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 4532
FspI TGC'GCA
Cuts at: 2313 2313
Size: 4532
HaeII r_GCGC'y
Cuts at: 249 446 1183 1966 2214 3236 4215 249
Size: 197 737 783 248 1022 979 566
Fragments arranged by size:
1022 979 783 737 566 248 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 491 523 535 567 1284 1316 2374 2404 2406
Size: 32 12 32 717 32 1058 30 2
Cuts at: 2406 2436 491
Size: 30 2587
Fragments arranged by size:
2587 1058 717 32 32 32 30 30
12 2
HincII GTy'rAC
Cuts at: 1571 1571
Size: 4532
HindIII A'AGCT_T
Cuts at: 1043 1124 1043
Size: 81 4451
KasI G'GCGC_C
Cuts at: 1179 1962 2210 1179
Size: 783 248 3501
Fragments arranged by size:
3501 783 248
KpnI G_GTAC'C
Cuts at: 267 1552 267
Size: 1285 3247
MluI A'CGCG_T
Cuts at: 3965 3965
Size: 4532
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1418 3203 3387 1418
Size: 1785 184 2563
Fragments arranged by size:
2563 1785 184
MscI TGG'CCA
Cuts at: 2293 2293
Size: 4532
MslI CAynn'nnrTG
Cuts at: 1188 1356 1451 2521 2648 1188
Size: 168 95 1070 127 3072
Fragments arranged by size:
3072 1070 168 127 95
MspA1I CmG'CkG
Cuts at: 1217 1643 1957 2317 3330 3575 1217
Size: 426 314 360 1013 245 2174
Fragments arranged by size:
2174 1013 426 360 314 245
NaeI GCC'GGC
Cuts at: 2713 2713
Size: 4532
NarI GG'CG_CC
Cuts at: 1180 1963 2211 1180
Size: 783 248 3501
Fragments arranged by size:
3501 783 248
NgoMIV G'CCGG_C
Cuts at: 2711 2711
Size: 4532
NspI r_CATG'y
Cuts at: 591 1309 1585 2085 2992 591
Size: 718 276 500 907 2131
Fragments arranged by size:
2131 907 718 500 276
PciI A'CATG_T
Cuts at: 2988 2988
Size: 4532
PfoI T'CCnGG_A
Cuts at: 359 359
Size: 4532
PmeI GTTT'AAAC
Cuts at: 1531 1531
Size: 4532
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 655 687 887 919 3671 3703 655
Size: 32 200 32 2752 32 1484
Fragments arranged by size:
2752 1484 200 32 32 32
PspOMI G'GGCC_C
Cuts at: 144 634 144
Size: 490 4042
PstI C_TGCA'G
Cuts at: 1579 2264 1579
Size: 685 3847
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4532
PvuII CAG'CTG
Cuts at: 1957 2317 1957
Size: 360 4172
RsrII CG'GwC_CG
Cuts at: 2727 2727
Size: 4532
SacI G_AGCT'C
Cuts at: 410 1141 1546 410
Size: 731 405 3396
Fragments arranged by size:
3396 731 405
SalI G'TCGA_C
Cuts at: 1569 1569
Size: 4532
SapI GCTCTTCn'nnn_
Cuts at: 1285 2555 2765 1285
Size: 1270 210 3052
Fragments arranged by size:
3052 1270 210
SbfI CC_TGCA'GG
Cuts at: 1579 1579
Size: 4532
ScaI AGT'ACT
Cuts at: 4049 4049
Size: 4532
SexAI A'CCwGG_T
Cuts at: 503 503
Size: 4532
SfcI C'TryA_G
Cuts at: 33 1077 1467 1575 2260 3253 3444 33
Size: 1044 390 108 685 993 191 1121
Fragments arranged by size:
1121 1044 993 685 390 191 108
SfoI GGC'GCC
Cuts at: 1181 1964 2212 1181
Size: 783 248 3501
Fragments arranged by size:
3501 783 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4532
SmaI CCC'GGG
Cuts at: 1554 1554
Size: 4532
SmlI C'TyrA_G
Cuts at: 677 1687 2602 3094 3356 677
Size: 1010 915 492 262 1853
Fragments arranged by size:
1853 1010 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4532
SphI G_CATG'C
Cuts at: 591 1309 1585 2085 591
Size: 718 276 500 3038
Fragments arranged by size:
3038 718 500 276
StuI AGG'CCT
Cuts at: 153 415 153
Size: 262 4270
StyI C'CwwG_G
Cuts at: 116 148 347 602 1666 2020 116
Size: 32 199 255 1064 354 2628
Fragments arranged by size:
2628 1064 354 255 199 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 408 2115 2248 2707 2916 408
Size: 1707 133 459 209 2024
Fragments arranged by size:
2024 1707 459 209 133
TatI w'GTAC_w
Cuts at: 641 4047 641
Size: 3406 1126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4049 4081 4049
Size: 32 4500
Tth111I GACn'n_nGTC
Cuts at: 2329 2329
Size: 4532
XbaI T'CTAG_A
Cuts at: 1563 1563
Size: 4532
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 274 2027 274
Size: 1753 2779
XmaI C'CCGG_G
Cuts at: 1552 1552
Size: 4532
XmnI GAAnn'nnTTC
Cuts at: 902 1045 1294 1378 902
Size: 143 249 84 4056
Fragments arranged by size:
4056 249 143 84
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AleI AlfI AscI BaeI BbsI BfrBI
BglI BmgBI BmtI BsiWI BssHII BstEII BstZ17I BtgI
ClaI FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI