Restriction map for
pF1KB8294
(Circular) (Six-base) MAPSORT of: pF1KB8294.seq Check: 5697 from: 1 to: 5186
pF1KB8294 5186 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 2224 2224
Size: 5186
Acc65I G'GTAC_C
Cuts at: 2020 2202 2020
Size: 182 5004
AclI AA'CG_TT
Cuts at: 5026 5026
Size: 5186
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 1027 1292 1408 1925 3011 3443 4190 4458
Size: 402 265 116 517 1086 432 747 268
Cuts at: 4458 625
Size: 1353
Fragments arranged by size:
1353 1086 747 517 432 402 268 265
116
AfeI AGC'GCT
Cuts at: 2092 2092
Size: 5186
AflIII A'CryG_T
Cuts at: 2538 3642 4619 2538
Size: 1104 977 3105
Fragments arranged by size:
3105 1104 977
AgeI A'CCGG_T
Cuts at: 3574 3574
Size: 5186
AleI CACnn'nnGTG
Cuts at: 193 298 350 193
Size: 105 52 5029
Fragments arranged by size:
5029 105 52
AlwNI CAG_nnn'CTG
Cuts at: 245 1056 2491 4058 245
Size: 811 1435 1567 1373
Fragments arranged by size:
1567 1435 1373 811
ApaI G_GGCC'C
Cuts at: 1152 2050 1152
Size: 898 4288
ApaLI G'TGCA_C
Cuts at: 3956 3956
Size: 5186
ApoI r'AATT_y
Cuts at: 996 2190 4539 4795 996
Size: 1194 2349 256 1387
Fragments arranged by size:
2349 1387 1194 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5186
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5186
AvaI C'yCGr_G
Cuts at: 658 893 1472 1808 2206 658
Size: 235 579 336 398 3638
Fragments arranged by size:
3638 579 398 336 235
AvrII C'CTAG_G
Cuts at: 1089 1089
Size: 5186
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1486 1519 1486
Size: 33 5153
BamHI G'GATC_C
Cuts at: 2211 2211
Size: 5186
BanI G'GyrC_C
Cuts at: 132 223 2020 2202 2616 2864 2899 132
Size: 91 1797 182 414 248 35 2419
Fragments arranged by size:
2419 1797 414 248 182 91 35
BanII G_rGCy'C
Cuts at: 393 448 517 659 669 965 1127 1152 1298
Size: 55 69 142 10 296 162 25 146
Cuts at: 1298 2050 2200 3230 393
Size: 752 150 1030 2349
Fragments arranged by size:
2349 1030 752 296 162 150 146 142
69 55 25 10
BbeI G_GCGC'C
Cuts at: 2620 2868 2620
Size: 248 4938
BbsI GAAGACnn'nnnn_
Cuts at: 299 1748 299
Size: 1449 3737
BbvCI CC'TCA_GC
Cuts at: 637 1877 637
Size: 1240 3946
BciVI GTATCCnnnnn_n'
Cuts at: 3084 3845 5186 3084
Size: 761 1341 3084
Fragments arranged by size:
3084 1341 761
BclI T'GATC_A
Cuts at: 2706 2706
Size: 5186
BglII A'GATC_T
Cuts at: 2701 4738 2701
Size: 2037 3149
BlpI GC'TnA_GC
Cuts at: 392 959 1049 1292 1696 2298 392
Size: 567 90 243 404 602 3280
Fragments arranged by size:
3280 602 567 404 243 90
Bme1580I G_kGCm'C
Cuts at: 135 1152 2050 2811 2904 3960 135
Size: 1017 898 761 93 1056 1361
Fragments arranged by size:
1361 1056 1017 898 761 93
BmrI ACTGGGnnnn_n'
Cuts at: 227 1987 2051 2398 2479 2814 4958 227
Size: 1760 64 347 81 335 2144 455
Fragments arranged by size:
2144 1760 455 347 335 81 64
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 412 3553 412
Size: 3141 2045
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 637 820 1141 1537 1648 2143 517
Size: 120 183 321 396 111 495 3560
Fragments arranged by size:
3560 495 396 321 183 120 111
Bpu10I CC'TnA_GC
Cuts at: 637 763 1043 1877 637
Size: 126 280 834 3946
Fragments arranged by size:
3946 834 280 126
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1152 2362 3241 3733 4031 1152
Size: 1210 879 492 298 2307
Fragments arranged by size:
2307 1210 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 773 1775 4890 773
Size: 1002 3115 1069
Fragments arranged by size:
3115 1069 1002
BsaAI yAC'GTr
Cuts at: 2539 2539
Size: 5186
BsaBI GATnn'nnATC
Cuts at: 6 253 2700 4737 6
Size: 247 2447 2037 455
Fragments arranged by size:
2447 2037 455 247
BsaHI Gr'CG_yC
Cuts at: 2617 2865 3567 5063 2617
Size: 248 702 1496 2740
Fragments arranged by size:
2740 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2375 2896 3574 3848 3995 4406 2375
Size: 521 678 274 147 411 3155
Fragments arranged by size:
3155 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 770 800 1774 1804 770
Size: 30 974 30 4152
Fragments arranged by size:
4152 974 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 514 654 1045 1213 1447 1594 1693 1782 1847
Size: 140 391 168 234 147 99 89 65
Cuts at: 1847 1966 514
Size: 119 3734
Fragments arranged by size:
3734 391 234 168 147 140 119 99
89 65
BseYI C'CCAG_C
Cuts at: 1100 1801 2611 3369 3946 4442 1100
Size: 701 810 758 577 496 1844
Fragments arranged by size:
1844 810 758 701 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 1418 1957 670
Size: 748 539 3899
Fragments arranged by size:
3899 748 539
BsiEI CG_ry'CG
Cuts at: 104 2728 2774 3982 104
Size: 2624 46 1208 1308
Fragments arranged by size:
2624 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 393 659 692 965 1044 1298 1553 2200 2978
Size: 266 33 273 79 254 255 647 778
Cuts at: 2978 3168 3960 393
Size: 190 792 1619
Fragments arranged by size:
1619 792 778 647 273 266 255 254
190 79 33
Bsp1286I G_dGCh'C
Cuts at: 135 393 448 517 659 669 692 965 1044
Size: 258 55 69 142 10 23 273 79
Cuts at: 1044 1127 1152 1298 1553 2050 2200 2811 2904
Size: 83 25 146 255 497 150 611 93
Cuts at: 2904 2978 3168 3230 3960 135
Size: 74 190 62 730 1361
Fragments arranged by size:
1361 730 611 497 273 258 255 190
150 146 142 93 83 79 74 69
62 55 25 23 10
BspEI T'CCGG_A
Cuts at: 4406 4406
Size: 5186
BspHI T'CATG_A
Cuts at: 4362 4362
Size: 5186
BspMI ACCTGCnnnn'nnnn_
Cuts at: 383 2236 2450 2752 3133 383
Size: 1853 214 302 381 2436
Fragments arranged by size:
2436 1853 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1341 3478 3532 5016 5181 50
Size: 1291 2137 54 1484 165 55
Fragments arranged by size:
2137 1484 1291 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3098 3098
Size: 5186
BsrFI r'CCGG_y
Cuts at: 767 1842 3184 3365 3574 767
Size: 1075 1342 181 209 2379
Fragments arranged by size:
2379 1342 1075 209 181
BssSI C'ACGA_G
Cuts at: 3457 3815 3457
Size: 358 4828
BstAPI GCAn_nnn'nTGC
Cuts at: 2563 2563
Size: 5186
BstBI TT'CG_AA
Cuts at: 3547 3547
Size: 5186
BstEII G'GTnAC_C
Cuts at: 489 489
Size: 5186
BstXI CCAn_nnnn'nTGG
Cuts at: 436 2137 436
Size: 1701 3485
BstYI r'GATC_y
Cuts at: 1 1382 2211 2678 2701 3036 3282 4294 4380
Size: 1381 829 467 23 335 246 1012 86
Cuts at: 4380 4392 4738 1
Size: 12 346 449
Fragments arranged by size:
1381 1012 829 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 141 476 732 107
Size: 34 335 256 4561
Fragments arranged by size:
4561 335 256 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3136 3317 3480 3136
Size: 181 163 4842
Fragments arranged by size:
4842 181 163
BtsI GCAGTG_nn'
Cuts at: 1989 2429 1989
Size: 440 4746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1173 1208 1173
Size: 35 5151
DraI TTT'AAA
Cuts at: 2185 2185
Size: 5186
DrdI GACnn_nn'nnGTC
Cuts at: 2892 3750 2892
Size: 858 4328
EaeI y'GGCC_r
Cuts at: 1112 1205 2000 2771 2945 3336 3363 4708 1112
Size: 93 795 771 174 391 27 1345 1590
Fragments arranged by size:
1590 1345 795 771 391 174 93 27
EagI C'GGCC_G
Cuts at: 2771 2771
Size: 5186
EarI CTCTTCn'nnn_
Cuts at: 743 1012 3209 3419 743
Size: 269 2197 210 2510
Fragments arranged by size:
2510 2197 269 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3394 3704 3850 4996 3394
Size: 310 146 1146 3584
Fragments arranged by size:
3584 1146 310 146
EcoICRI GAG'CTC
Cuts at: 391 657 963 1296 2198 391
Size: 266 306 333 902 3379
Fragments arranged by size:
3379 902 333 306 266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 625 637 820 1027 1141 1292 1408 1537
Size: 108 12 183 207 114 151 116 129
Cuts at: 1537 1648 1925 2143 3011 3443 4190 4458 517
Size: 111 277 218 868 432 747 268 1245
Fragments arranged by size:
1245 868 747 432 277 268 218 207
183 151 129 116 114 111 108 12
EcoNI CCTnn'n_nnAGG
Cuts at: 499 1009 1093 1630 499
Size: 510 84 537 4055
Fragments arranged by size:
4055 537 510 84
EcoO109I rG'GnC_Cy
Cuts at: 885 1148 1439 2046 2047 2163 2325 885
Size: 263 291 607 1 116 162 3746
Fragments arranged by size:
3746 607 291 263 162 116 1
EcoRI G'AATT_C
Cuts at: 2190 2190
Size: 5186
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1204 2109 3205 1204
Size: 905 1096 3185
Fragments arranged by size:
3185 1096 905
FspI TGC'GCA
Cuts at: 2967 2967
Size: 5186
HaeII r_GCGC'y
Cuts at: 2094 2620 2868 3890 4869 2094
Size: 526 248 1022 979 2411
Fragments arranged by size:
2411 1022 979 526 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 499 531 1519 1551 1717 1749 1774 1806 1873
Size: 32 988 32 166 32 25 32 67
Cuts at: 1873 1900 1905 1932 3028 3058 3060 3090 499
Size: 27 5 27 1096 30 2 30 2595
Fragments arranged by size:
2595 1096 988 166 67 32 32 32
32 30 30 27 27 25 5 2
HincII GTy'rAC
Cuts at: 153 1278 2225 153
Size: 1125 947 3114
Fragments arranged by size:
3114 1125 947
HpaI GTT'AAC
Cuts at: 1278 1278
Size: 5186
KasI G'GCGC_C
Cuts at: 2616 2864 2616
Size: 248 4938
KpnI G_GTAC'C
Cuts at: 2024 2206 2024
Size: 182 5004
MluI A'CGCG_T
Cuts at: 4619 4619
Size: 5186
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3857 4041 3857
Size: 184 5002
MscI TGG'CCA
Cuts at: 1207 2002 2947 1207
Size: 795 945 3446
Fragments arranged by size:
3446 945 795
MslI CAynn'nnrTG
Cuts at: 193 298 300 350 2135 3175 3302 193
Size: 105 2 50 1785 1040 127 2077
Fragments arranged by size:
2077 1785 1040 127 105 50 2
MspA1I CmG'CkG
Cuts at: 603 615 1107 1687 1876 1933 2297 2611 2971
Size: 12 492 580 189 57 364 314 360
Cuts at: 2971 3984 4229 603
Size: 1013 245 1560
Fragments arranged by size:
1560 1013 580 492 364 360 314 245
189 57 12
NaeI GCC'GGC
Cuts at: 769 1844 3367 769
Size: 1075 1523 2588
Fragments arranged by size:
2588 1523 1075
NarI GG'CG_CC
Cuts at: 2617 2865 2617
Size: 248 4938
NcoI C'CATG_G
Cuts at: 107 476 732 107
Size: 369 256 4561
Fragments arranged by size:
4561 369 256
NgoMIV G'CCGG_C
Cuts at: 767 1842 3365 767
Size: 1075 1523 2588
Fragments arranged by size:
2588 1523 1075
NspI r_CATG'y
Cuts at: 2239 2739 3646 2239
Size: 500 907 3779
Fragments arranged by size:
3779 907 500
PciI A'CATG_T
Cuts at: 3642 3642
Size: 5186
PflMI CCAn_nnn'nTGG
Cuts at: 142 245 1762 142
Size: 103 1517 3566
Fragments arranged by size:
3566 1517 103
PfoI T'CCnGG_A
Cuts at: 797 797
Size: 5186
PmeI GTTT'AAAC
Cuts at: 2185 2185
Size: 5186
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4325 4357 4325
Size: 32 5154
PpuMI rG'GwC_Cy
Cuts at: 1439 2163 1439
Size: 724 4462
PspOMI G'GGCC_C
Cuts at: 1148 2046 1148
Size: 898 4288
PstI C_TGCA'G
Cuts at: 1358 2233 2918 1358
Size: 875 685 3626
Fragments arranged by size:
3626 875 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5186
PvuII CAG'CTG
Cuts at: 603 615 1876 1933 2611 2971 603
Size: 12 1261 57 678 360 2818
Fragments arranged by size:
2818 1261 678 360 57 12
RsrII CG'GwC_CG
Cuts at: 3381 3381
Size: 5186
SacI G_AGCT'C
Cuts at: 393 659 965 1298 2200 393
Size: 266 306 333 902 3379
Fragments arranged by size:
3379 902 333 306 266
SalI G'TCGA_C
Cuts at: 2223 2223
Size: 5186
SapI GCTCTTCn'nnn_
Cuts at: 1012 3209 3419 1012
Size: 2197 210 2779
Fragments arranged by size:
2779 2197 210
SbfI CC_TGCA'GG
Cuts at: 2233 2233
Size: 5186
ScaI AGT'ACT
Cuts at: 4703 4703
Size: 5186
SfcI C'TryA_G
Cuts at: 33 610 1354 2229 2914 3907 4098 33
Size: 577 744 875 685 993 191 1121
Fragments arranged by size:
1121 993 875 744 685 577 191
SfoI GGC'GCC
Cuts at: 2618 2866 2618
Size: 248 4938
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5186
SmaI CCC'GGG
Cuts at: 1474 2208 1474
Size: 734 4452
SmlI C'TyrA_G
Cuts at: 658 1167 1808 2341 3256 3748 4010 658
Size: 509 641 533 915 492 262 1834
Fragments arranged by size:
1834 915 641 533 509 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5186
SphI G_CATG'C
Cuts at: 2239 2739 2239
Size: 500 4686
StyI C'CwwG_G
Cuts at: 107 191 469 476 539 732 912 1089 2320
Size: 84 278 7 63 193 180 177 1231
Cuts at: 2320 2674 107
Size: 354 2619
Fragments arranged by size:
2619 1231 354 278 193 180 177 84
63 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2173 2769 2902 3361 3570 2173
Size: 596 133 459 209 3789
Fragments arranged by size:
3789 596 459 209 133
TatI w'GTAC_w
Cuts at: 322 710 930 1601 4701 322
Size: 388 220 671 3100 807
Fragments arranged by size:
3100 807 671 388 220
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 770 802 4703 4735 770
Size: 32 3901 32 1221
Fragments arranged by size:
3901 1221 32 32
Tth111I GACn'n_nGTC
Cuts at: 2983 2983
Size: 5186
XbaI T'CTAG_A
Cuts at: 2217 2217
Size: 5186
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 192 483 2681 192
Size: 291 2198 2697
Fragments arranged by size:
2697 2198 291
XhoI C'TCGA_G
Cuts at: 658 1808 658
Size: 1150 4036
XmaI C'CCGG_G
Cuts at: 1472 2206 1472
Size: 734 4452
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI AscI BcgI
BfrBI BglI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI
HindIII MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XmnI ZraI