Restriction map for
pF1KB8293
(Circular) (Six-base) MAPSORT of: pF1KB8293.seq Check: 3024 from: 1 to: 4019
pF1KB8293 4019 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 621 1057 621
Size: 436 3583
Acc65I G'GTAC_C
Cuts at: 1035 1035
Size: 4019
AclI AA'CG_TT
Cuts at: 780 3859 780
Size: 3079 940
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1844 2276 3023 3291 1844
Size: 432 747 268 2572
Fragments arranged by size:
2572 747 432 268
AflII C'TTAA_G
Cuts at: 817 817
Size: 4019
AflIII A'CryG_T
Cuts at: 1371 2475 3452 1371
Size: 1104 977 1938
Fragments arranged by size:
1938 1104 977
AgeI A'CCGG_T
Cuts at: 2407 2407
Size: 4019
AlwNI CAG_nnn'CTG
Cuts at: 862 1324 2891 862
Size: 462 1567 1990
Fragments arranged by size:
1990 1567 462
ApaLI G'TGCA_C
Cuts at: 2789 2789
Size: 4019
ApoI r'AATT_y
Cuts at: 377 927 1023 3372 3628 377
Size: 550 96 2349 256 768
Fragments arranged by size:
2349 768 550 256 96
AseI AT'TA_AT
Cuts at: 20 20
Size: 4019
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4019
AvaI C'yCGr_G
Cuts at: 1010 1039 1010
Size: 29 3990
BamHI G'GATC_C
Cuts at: 190 1044 190
Size: 854 3165
BanI G'GyrC_C
Cuts at: 1035 1449 1697 1732 1035
Size: 414 248 35 3322
Fragments arranged by size:
3322 414 248 35
BanII G_rGCy'C
Cuts at: 277 1033 2063 277
Size: 756 1030 2233
Fragments arranged by size:
2233 1030 756
BbeI G_GCGC'C
Cuts at: 1453 1701 1453
Size: 248 3771
BbsI GAAGACnn'nnnn_
Cuts at: 803 841 803
Size: 38 3981
BciVI GTATCCnnnnn_n'
Cuts at: 1917 2678 4019 1917
Size: 761 1341 1917
Fragments arranged by size:
1917 1341 761
BclI T'GATC_A
Cuts at: 1539 1539
Size: 4019
BglII A'GATC_T
Cuts at: 363 726 1534 3571 363
Size: 363 808 2037 811
Fragments arranged by size:
2037 811 808 363
BlpI GC'TnA_GC
Cuts at: 1131 1131
Size: 4019
Bme1580I G_kGCm'C
Cuts at: 1644 1737 2793 1644
Size: 93 1056 2870
Fragments arranged by size:
2870 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1231 1312 1647 3791 1231
Size: 81 335 2144 1459
Fragments arranged by size:
2144 1459 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 4019
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 901
Size: 4019
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 1195 2074 2566 2864 198
Size: 997 879 492 298 1353
Fragments arranged by size:
1353 997 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3723 3723
Size: 4019
BsaAI yAC'GTr
Cuts at: 1372 1372
Size: 4019
BsaBI GATnn'nnATC
Cuts at: 6 1533 3570 6
Size: 1527 2037 455
Fragments arranged by size:
2037 1527 455
BsaHI Gr'CG_yC
Cuts at: 1450 1698 2400 3896 1450
Size: 248 702 1496 1573
Fragments arranged by size:
1573 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1208 1729 2407 2681 2828 3239 1208
Size: 521 678 274 147 411 1988
Fragments arranged by size:
1988 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1444 2202 2779 3275 1444
Size: 758 577 496 2188
Fragments arranged by size:
2188 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 529 425
Size: 104 3915
BsiEI CG_ry'CG
Cuts at: 104 1561 1607 2815 104
Size: 1457 46 1208 1308
Fragments arranged by size:
1457 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 227 1033 1811 2001 2793 227
Size: 806 778 190 792 1453
Fragments arranged by size:
1453 806 792 778 190
BsmI GAATG_Cn'
Cuts at: 767 987 767
Size: 220 3799
Bsp1286I G_dGCh'C
Cuts at: 227 277 1033 1644 1737 1811 2001 2063 2793
Size: 50 756 611 93 74 190 62 730
Cuts at: 2793 227
Size: 1453
Fragments arranged by size:
1453 756 730 611 190 93 74 62
50
BspEI T'CCGG_A
Cuts at: 3239 3239
Size: 4019
BspHI T'CATG_A
Cuts at: 3195 3195
Size: 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1069 1283 1585 1966 1069
Size: 214 302 381 3122
Fragments arranged by size:
3122 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2311 2365 3849 4014 50
Size: 2261 54 1484 165 55
Fragments arranged by size:
2261 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 344 1931 344
Size: 1587 2432
BsrFI r'CCGG_y
Cuts at: 2017 2198 2407 2017
Size: 181 209 3629
Fragments arranged by size:
3629 209 181
BssSI C'ACGA_G
Cuts at: 2290 2648 2290
Size: 358 3661
BstAPI GCAn_nnn'nTGC
Cuts at: 1396 1396
Size: 4019
BstBI TT'CG_AA
Cuts at: 569 612 2380 569
Size: 43 1768 2208
Fragments arranged by size:
2208 1768 43
BstXI CCAn_nnnn'nTGG
Cuts at: 879 879
Size: 4019
BstYI r'GATC_y
Cuts at: 1 190 363 726 1044 1511 1534 1869 2115
Size: 189 173 363 318 467 23 335 246
Cuts at: 2115 3127 3213 3225 3571 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 363 346 335 318 246
189 173 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4019
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1969 2150 2313 1969
Size: 181 163 3675
Fragments arranged by size:
3675 181 163
BtsI GCAGTG_nn'
Cuts at: 136 1262 136
Size: 1126 2893
DraI TTT'AAA
Cuts at: 1018 1018
Size: 4019
DraIII CAC_nnn'GTG
Cuts at: 448 448
Size: 4019
DrdI GACnn_nn'nnGTC
Cuts at: 1725 2583 1725
Size: 858 3161
EaeI y'GGCC_r
Cuts at: 1604 1778 2169 2196 3541 1604
Size: 174 391 27 1345 2082
Fragments arranged by size:
2082 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1604 1604
Size: 4019
EarI CTCTTCn'nnn_
Cuts at: 398 2042 2252 398
Size: 1644 210 2165
Fragments arranged by size:
2165 1644 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 200 2227 2537 2683 3829 200
Size: 2027 310 146 1146 390
Fragments arranged by size:
2027 1146 390 310 146
EcoICRI GAG'CTC
Cuts at: 1031 1031
Size: 4019
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 1844 2276 3023 3291 901
Size: 943 432 747 268 1629
Fragments arranged by size:
1629 943 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1158 1158
Size: 4019
EcoRI G'AATT_C
Cuts at: 1023 1023
Size: 4019
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 4019
FspI TGC'GCA
Cuts at: 1800 1800
Size: 4019
HaeII r_GCGC'y
Cuts at: 1453 1701 2723 3702 1453
Size: 248 1022 979 1770
Fragments arranged by size:
1770 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 556 588 703 735 1861 1891 1893 1923 556
Size: 32 115 32 1126 30 2 30 2652
Fragments arranged by size:
2652 1126 115 32 32 30 30 2
HincII GTy'rAC
Cuts at: 318 1058 318
Size: 740 3279
HindIII A'AGCT_T
Cuts at: 428 428
Size: 4019
KasI G'GCGC_C
Cuts at: 1449 1697 1449
Size: 248 3771
KpnI G_GTAC'C
Cuts at: 1039 1039
Size: 4019
MluI A'CGCG_T
Cuts at: 3452 3452
Size: 4019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 551 2690 2874 551
Size: 2139 184 1696
Fragments arranged by size:
2139 1696 184
MscI TGG'CCA
Cuts at: 1780 1780
Size: 4019
MslI CAynn'nnrTG
Cuts at: 2008 2135 2008
Size: 127 3892
MspA1I CmG'CkG
Cuts at: 1130 1444 1804 2817 3062 1130
Size: 314 360 1013 245 2087
Fragments arranged by size:
2087 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2200 2200
Size: 4019
NarI GG'CG_CC
Cuts at: 1450 1698 1450
Size: 248 3771
NcoI C'CATG_G
Cuts at: 107 107
Size: 4019
NgoMIV G'CCGG_C
Cuts at: 2198 2198
Size: 4019
NspI r_CATG'y
Cuts at: 212 1072 1572 2479 212
Size: 860 500 907 1752
Fragments arranged by size:
1752 907 860 500
PciI A'CATG_T
Cuts at: 2475 2475
Size: 4019
PmeI GTTT'AAAC
Cuts at: 1018 1018
Size: 4019
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 765 797 3158 3190 765
Size: 32 2361 32 1594
Fragments arranged by size:
2361 1594 32 32
PspXI vC'TCGA_Gb
Cuts at: 1010 1010
Size: 4019
PstI C_TGCA'G
Cuts at: 170 1066 1751 170
Size: 896 685 2438
Fragments arranged by size:
2438 896 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4019
PvuII CAG'CTG
Cuts at: 1444 1804 1444
Size: 360 3659
RsrII CG'GwC_CG
Cuts at: 2214 2214
Size: 4019
SacI G_AGCT'C
Cuts at: 1033 1033
Size: 4019
SalI G'TCGA_C
Cuts at: 1056 1056
Size: 4019
SapI GCTCTTCn'nnn_
Cuts at: 2042 2252 2042
Size: 210 3809
SbfI CC_TGCA'GG
Cuts at: 1066 1066
Size: 4019
ScaI AGT'ACT
Cuts at: 3536 3536
Size: 4019
SfcI C'TryA_G
Cuts at: 33 166 638 1062 1747 2740 2931 33
Size: 133 472 424 685 993 191 1121
Fragments arranged by size:
1121 993 685 472 424 191 133
SfoI GGC'GCC
Cuts at: 1451 1699 1451
Size: 248 3771
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4019
SmaI CCC'GGG
Cuts at: 1041 1041
Size: 4019
SmlI C'TyrA_G
Cuts at: 213 817 1010 1174 2089 2581 2843 213
Size: 604 193 164 915 492 262 1389
Fragments arranged by size:
1389 915 604 492 262 193 164
SpeI A'CTAG_T
Cuts at: 67 697 67
Size: 630 3389
SphI G_CATG'C
Cuts at: 1072 1572 1072
Size: 500 3519
StuI AGG'CCT
Cuts at: 294 294
Size: 4019
StyI C'CwwG_G
Cuts at: 107 1153 1507 107
Size: 1046 354 2619
Fragments arranged by size:
2619 1046 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1602 1735 2194 2403 1602
Size: 133 459 209 3218
Fragments arranged by size:
3218 459 209 133
TatI w'GTAC_w
Cuts at: 3534 3534
Size: 4019
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3536 3568 3536
Size: 32 3987
Tth111I GACn'n_nGTC
Cuts at: 1816 1816
Size: 4019
XbaI T'CTAG_A
Cuts at: 729 1050 729
Size: 321 3698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1514 1514
Size: 4019
XhoI C'TCGA_G
Cuts at: 1010 1010
Size: 4019
XmaI C'CCGG_G
Cuts at: 1039 1039
Size: 4019
XmnI GAAnn'nnTTC
Cuts at: 608 800 608
Size: 192 3827
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI ZraI