Restriction map for
pF1KB8292
(Circular) (Six-base) MAPSORT of: pF1KB8292.seq Check: 6531 from: 1 to: 4460
pF1KB8292 4460 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1498 1498
Size: 4460
Acc65I G'GTAC_C
Cuts at: 1476 1476
Size: 4460
AclI AA'CG_TT
Cuts at: 4300 4300
Size: 4460
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2285 2717 3464 3732 2285
Size: 432 747 268 3013
Fragments arranged by size:
3013 747 432 268
AflIII A'CryG_T
Cuts at: 383 1199 1812 2916 3893 383
Size: 816 613 1104 977 950
Fragments arranged by size:
1104 977 950 816 613
AgeI A'CCGG_T
Cuts at: 2848 2848
Size: 4460
AhdI GACnn_n'nnGTC
Cuts at: 672 801 672
Size: 129 4331
AleI CACnn'nnGTG
Cuts at: 260 1202 260
Size: 942 3518
AlwNI CAG_nnn'CTG
Cuts at: 1434 1765 3332 1434
Size: 331 1567 2562
Fragments arranged by size:
2562 1567 331
ApaLI G'TGCA_C
Cuts at: 1035 3230 1035
Size: 2195 2265
ApoI r'AATT_y
Cuts at: 208 244 1464 3813 4069 208
Size: 36 1220 2349 256 599
Fragments arranged by size:
2349 1220 599 256 36
AseI AT'TA_AT
Cuts at: 20 20
Size: 4460
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4460
AvaI C'yCGr_G
Cuts at: 654 1480 654
Size: 826 3634
BamHI G'GATC_C
Cuts at: 1485 1485
Size: 4460
BanI G'GyrC_C
Cuts at: 1476 1890 2138 2173 1476
Size: 414 248 35 3763
Fragments arranged by size:
3763 414 248 35
BanII G_rGCy'C
Cuts at: 1474 2504 1474
Size: 1030 3430
BbeI G_GCGC'C
Cuts at: 1894 2142 1894
Size: 248 4212
BbsI GAAGACnn'nnnn_
Cuts at: 964 1257 964
Size: 293 4167
BciVI GTATCCnnnnn_n'
Cuts at: 466 2358 3119 4460 466
Size: 1892 761 1341 466
Fragments arranged by size:
1892 1341 761 466
BclI T'GATC_A
Cuts at: 156 1980 156
Size: 1824 2636
BglI GCCn_nnn'nGGC
Cuts at: 946 946
Size: 4460
BglII A'GATC_T
Cuts at: 1975 4012 1975
Size: 2037 2423
BlpI GC'TnA_GC
Cuts at: 1572 1572
Size: 4460
Bme1580I G_kGCm'C
Cuts at: 1039 1143 2085 2178 3234 1039
Size: 104 942 93 1056 2265
Fragments arranged by size:
2265 1056 942 104 93
BmrI ACTGGGnnnn_n'
Cuts at: 1361 1672 1753 2088 4232 1361
Size: 311 81 335 2144 1589
Fragments arranged by size:
2144 1589 335 311 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2827 2827
Size: 4460
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1456 1456
Size: 4460
Bpu10I CC'TnA_GC
Cuts at: 691 691
Size: 4460
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 800 1219 1636 2515 3007 3305 331
Size: 469 419 417 879 492 298 1486
Fragments arranged by size:
1486 879 492 469 419 417 298
BsaI GGTCTCn'nnnn_
Cuts at: 772 4164 772
Size: 3392 1068
BsaAI yAC'GTr
Cuts at: 1813 1813
Size: 4460
BsaBI GATnn'nnATC
Cuts at: 6 1974 4011 6
Size: 1968 2037 455
Fragments arranged by size:
2037 1968 455
BsaHI Gr'CG_yC
Cuts at: 1891 2139 2841 4337 1891
Size: 248 702 1496 2014
Fragments arranged by size:
2014 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1649 2170 2848 3122 3269 3680 1649
Size: 521 678 274 147 411 2429
Fragments arranged by size:
2429 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 720 1885 2643 3220 3716 720
Size: 1165 758 577 496 1464
Fragments arranged by size:
1464 1165 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1272 1272
Size: 4460
BsiEI CG_ry'CG
Cuts at: 104 2002 2048 3256 104
Size: 1898 46 1208 1308
Fragments arranged by size:
1898 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 255 1039 1474 2252 2442 3234 255
Size: 784 435 778 190 792 1481
Fragments arranged by size:
1481 792 784 778 435 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1432 1432
Size: 4460
Bsp1286I G_dGCh'C
Cuts at: 255 1039 1143 1474 2085 2178 2252 2442 2504
Size: 784 104 331 611 93 74 190 62
Cuts at: 2504 3234 255
Size: 730 1481
Fragments arranged by size:
1481 784 730 611 331 190 104 93
74 62
BspEI T'CCGG_A
Cuts at: 3680 3680
Size: 4460
BspHI T'CATG_A
Cuts at: 1242 3636 1242
Size: 2394 2066
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1347 1510 1724 2026 2407 1347
Size: 163 214 302 381 3400
Fragments arranged by size:
3400 381 302 214 163
BsrBI CCG'CTC
Cuts at: 50 2752 2806 4290 4455 50
Size: 2702 54 1484 165 55
Fragments arranged by size:
2702 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2372 2372
Size: 4460
BsrFI r'CCGG_y
Cuts at: 2458 2639 2848 2458
Size: 181 209 4070
Fragments arranged by size:
4070 209 181
BsrGI T'GTAC_A
Cuts at: 386 386
Size: 4460
BssSI C'ACGA_G
Cuts at: 1327 2731 3089 1327
Size: 1404 358 2698
Fragments arranged by size:
2698 1404 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1837 1837
Size: 4460
BstBI TT'CG_AA
Cuts at: 2821 2821
Size: 4460
BstEII G'GTnAC_C
Cuts at: 919 1359 919
Size: 440 4020
BstXI CCAn_nnnn'nTGG
Cuts at: 952 952
Size: 4460
BstYI r'GATC_y
Cuts at: 1 1485 1952 1975 2310 2556 3568 3654 3666
Size: 1484 467 23 335 246 1012 86 12
Cuts at: 3666 4012 1
Size: 346 449
Fragments arranged by size:
1484 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 773 107
Size: 666 3794
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1423 2410 2591 2754 1423
Size: 987 181 163 3129
Fragments arranged by size:
3129 987 181 163
BtsI GCAGTG_nn'
Cuts at: 1703 1703
Size: 4460
DraI TTT'AAA
Cuts at: 291 1459 291
Size: 1168 3292
DrdI GACnn_nn'nnGTC
Cuts at: 2166 3024 2166
Size: 858 3602
EaeI y'GGCC_r
Cuts at: 756 2045 2219 2610 2637 3982 756
Size: 1289 174 391 27 1345 1234
Fragments arranged by size:
1345 1289 1234 391 174 27
EagI C'GGCC_G
Cuts at: 2045 2045
Size: 4460
EarI CTCTTCn'nnn_
Cuts at: 362 2483 2693 362
Size: 2121 210 2129
Fragments arranged by size:
2129 2121 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1024 2668 2978 3124 4270 1024
Size: 1644 310 146 1146 1214
Fragments arranged by size:
1644 1214 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1472 1472
Size: 4460
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1456 2285 2717 3464 3732 1456
Size: 829 432 747 268 2184
Fragments arranged by size:
2184 829 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 372 803 1599 372
Size: 431 796 3233
Fragments arranged by size:
3233 796 431
EcoRI G'AATT_C
Cuts at: 1464 1464
Size: 4460
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 4460
FspI TGC'GCA
Cuts at: 2241 2241
Size: 4460
HaeII r_GCGC'y
Cuts at: 1894 2142 3164 4143 1894
Size: 248 1022 979 2211
Fragments arranged by size:
2211 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 658 690 787 819 2302 2332 2334 2364 658
Size: 32 97 32 1483 30 2 30 2754
Fragments arranged by size:
2754 1483 97 32 32 30 30 2
HincII GTy'rAC
Cuts at: 423 1499 423
Size: 1076 3384
HindIII A'AGCT_T
Cuts at: 295 307 295
Size: 12 4448
HpaI GTT'AAC
Cuts at: 423 423
Size: 4460
KasI G'GCGC_C
Cuts at: 1890 2138 1890
Size: 248 4212
KpnI G_GTAC'C
Cuts at: 1480 1480
Size: 4460
MfeI C'AATT_G
Cuts at: 747 747
Size: 4460
MluI A'CGCG_T
Cuts at: 3893 3893
Size: 4460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3131 3315 3131
Size: 184 4276
MscI TGG'CCA
Cuts at: 2221 2221
Size: 4460
MslI CAynn'nnrTG
Cuts at: 124 258 260 1175 1202 2449 2576 124
Size: 134 2 915 27 1247 127 2008
Fragments arranged by size:
2008 1247 915 134 127 27 2
MspA1I CmG'CkG
Cuts at: 1434 1571 1885 2245 3258 3503 1434
Size: 137 314 360 1013 245 2391
Fragments arranged by size:
2391 1013 360 314 245 137
NaeI GCC'GGC
Cuts at: 2641 2641
Size: 4460
NarI GG'CG_CC
Cuts at: 1891 2139 1891
Size: 248 4212
NcoI C'CATG_G
Cuts at: 107 773 107
Size: 666 3794
NgoMIV G'CCGG_C
Cuts at: 2639 2639
Size: 4460
NspI r_CATG'y
Cuts at: 387 1203 1513 2013 2920 387
Size: 816 310 500 907 1927
Fragments arranged by size:
1927 907 816 500 310
PciI A'CATG_T
Cuts at: 383 1199 2916 383
Size: 816 1717 1927
Fragments arranged by size:
1927 1717 816
PmeI GTTT'AAAC
Cuts at: 1459 1459
Size: 4460
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3599 3631 3599
Size: 32 4428
PpuMI rG'GwC_Cy
Cuts at: 372 803 372
Size: 431 4029
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 370 402 370
Size: 32 4428
PstI C_TGCA'G
Cuts at: 280 1507 2192 280
Size: 1227 685 2548
Fragments arranged by size:
2548 1227 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4460
PvuII CAG'CTG
Cuts at: 1434 1885 2245 1434
Size: 451 360 3649
Fragments arranged by size:
3649 451 360
RsrII CG'GwC_CG
Cuts at: 2655 2655
Size: 4460
SacI G_AGCT'C
Cuts at: 1474 1474
Size: 4460
SalI G'TCGA_C
Cuts at: 1497 1497
Size: 4460
SanDI GG'GwC_CC
Cuts at: 803 803
Size: 4460
SapI GCTCTTCn'nnn_
Cuts at: 2483 2693 2483
Size: 210 4250
SbfI CC_TGCA'GG
Cuts at: 1507 1507
Size: 4460
ScaI AGT'ACT
Cuts at: 3977 3977
Size: 4460
SfcI C'TryA_G
Cuts at: 33 276 1104 1449 1503 2188 3181 3372 33
Size: 243 828 345 54 685 993 191 1121
Fragments arranged by size:
1121 993 828 685 345 243 191 54
SfoI GGC'GCC
Cuts at: 1892 2140 1892
Size: 248 4212
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4460
SmaI CCC'GGG
Cuts at: 1482 1482
Size: 4460
SmlI C'TyrA_G
Cuts at: 310 815 1234 1615 2530 3022 3284 310
Size: 505 419 381 915 492 262 1486
Fragments arranged by size:
1486 915 505 492 419 381 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4460
SphI G_CATG'C
Cuts at: 1513 2013 1513
Size: 500 3960
StyI C'CwwG_G
Cuts at: 107 773 1292 1304 1594 1948 107
Size: 666 519 12 290 354 2619
Fragments arranged by size:
2619 666 519 354 290 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 318 595 2043 2176 2635 2844 318
Size: 277 1448 133 459 209 1934
Fragments arranged by size:
1934 1448 459 277 209 133
TatI w'GTAC_w
Cuts at: 386 3975 386
Size: 3589 871
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 460 492 3977 4009 460
Size: 32 3485 32 911
Fragments arranged by size:
3485 911 32 32
Tth111I GACn'n_nGTC
Cuts at: 2257 2257
Size: 4460
XbaI T'CTAG_A
Cuts at: 1491 1491
Size: 4460
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1955 1955
Size: 4460
XmaI C'CCGG_G
Cuts at: 1480 1480
Size: 4460
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BsaXI
BseRI BsiWI BsmI BssHII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI