Restriction map for
pF1KB8291
(Circular) (Six-base) MAPSORT of: pF1KB8291.seq Check: 6469 from: 1 to: 4529
pF1KB8291 4529 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 548 607 548
Size: 59 4470
AccI GT'mk_AC
Cuts at: 329 1463 1567 329
Size: 1134 104 3291
Fragments arranged by size:
3291 1134 104
Acc65I G'GTAC_C
Cuts at: 1545 1545
Size: 4529
AclI AA'CG_TT
Cuts at: 4369 4369
Size: 4529
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 462 2354 2786 3533 3801 462
Size: 1892 432 747 268 1190
Fragments arranged by size:
1892 1190 747 432 268
AfeI AGC'GCT
Cuts at: 773 773
Size: 4529
AflIII A'CryG_T
Cuts at: 1881 2985 3962 1881
Size: 1104 977 2448
Fragments arranged by size:
2448 1104 977
AgeI A'CCGG_T
Cuts at: 843 2917 843
Size: 2074 2455
AleI CACnn'nnGTG
Cuts at: 271 271
Size: 4529
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 289 1292 289
Size: 1003 3526
AlwNI CAG_nnn'CTG
Cuts at: 259 528 1180 1329 1834 3401 259
Size: 269 652 149 505 1567 1387
Fragments arranged by size:
1567 1387 652 505 269 149
ApaI G_GGCC'C
Cuts at: 656 656
Size: 4529
ApaLI G'TGCA_C
Cuts at: 3299 3299
Size: 4529
ApoI r'AATT_y
Cuts at: 708 1533 3882 4138 708
Size: 825 2349 256 1099
Fragments arranged by size:
2349 1099 825 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4529
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4529
AvaI C'yCGr_G
Cuts at: 1549 1549
Size: 4529
BamHI G'GATC_C
Cuts at: 1554 1554
Size: 4529
BanI G'GyrC_C
Cuts at: 335 1276 1545 1959 2207 2242 335
Size: 941 269 414 248 35 2622
Fragments arranged by size:
2622 941 414 269 248 35
BanII G_rGCy'C
Cuts at: 397 656 1543 2573 397
Size: 259 887 1030 2353
Fragments arranged by size:
2353 1030 887 259
BbeI G_GCGC'C
Cuts at: 1963 2211 1963
Size: 248 4281
BbvCI CC'TCA_GC
Cuts at: 252 768 252
Size: 516 4013
BciVI GTATCCnnnnn_n'
Cuts at: 2427 3188 4529 2427
Size: 761 1341 2427
Fragments arranged by size:
2427 1341 761
BclI T'GATC_A
Cuts at: 2049 2049
Size: 4529
BglI GCCn_nnn'nGGC
Cuts at: 1351 1351
Size: 4529
BglII A'GATC_T
Cuts at: 2044 4081 2044
Size: 2037 2492
BlpI GC'TnA_GC
Cuts at: 1641 1641
Size: 4529
Bme1580I G_kGCm'C
Cuts at: 278 285 656 1281 2154 2247 3303 278
Size: 7 371 625 873 93 1056 1504
Fragments arranged by size:
1504 1056 873 625 371 93 7
BmrI ACTGGGnnnn_n'
Cuts at: 272 1741 1822 2157 4301 272
Size: 1469 81 335 2144 500
Fragments arranged by size:
2144 1469 500 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2896 2896
Size: 4529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 565 571 760 1471 1540 328
Size: 237 6 189 711 69 3317
Fragments arranged by size:
3317 711 237 189 69 6
Bpu10I CC'TnA_GC
Cuts at: 252 634 768 252
Size: 382 134 4013
Fragments arranged by size:
4013 382 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1705 2584 3076 3374 1705
Size: 879 492 298 2860
Fragments arranged by size:
2860 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4233 4233
Size: 4529
BsaAI yAC'GTr
Cuts at: 1882 1882
Size: 4529
BsaBI GATnn'nnATC
Cuts at: 6 2043 4080 6
Size: 2037 2037 455
Fragments arranged by size:
2037 2037 455
BsaHI Gr'CG_yC
Cuts at: 1960 2208 2910 4406 1960
Size: 248 702 1496 2083
Fragments arranged by size:
2083 1496 702 248
BsaWI w'CCGG_w
Cuts at: 727 843 1718 2239 2917 3191 3338 3749 727
Size: 116 875 521 678 274 147 411 1507
Fragments arranged by size:
1507 875 678 521 411 274 147 116
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 658 683 688 713 925 955 1498 1528 658
Size: 25 5 25 212 30 543 30 3659
Fragments arranged by size:
3659 543 212 30 30 25 25 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 998 1447 1450 1453 1456 1459 1468 1471 998
Size: 449 3 3 3 3 9 3 4056
Fragments arranged by size:
4056 449 9 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 126 209 302 762 1954 2712 3289 3785 126
Size: 83 93 460 1192 758 577 496 870
Fragments arranged by size:
1192 870 758 577 496 460 93 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1361 1361
Size: 4529
BsiEI CG_ry'CG
Cuts at: 104 2071 2117 3325 104
Size: 1967 46 1208 1308
Fragments arranged by size:
1967 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 467 1543 2321 2511 3303 467
Size: 1076 778 190 792 1693
Fragments arranged by size:
1693 1076 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 278 285 397 467 656 1281 1543 2154 2247
Size: 7 112 70 189 625 262 611 93
Cuts at: 2247 2321 2511 2573 3303 278
Size: 74 190 62 730 1504
Fragments arranged by size:
1504 730 625 611 262 190 189 112
93 74 70 62 7
BspEI T'CCGG_A
Cuts at: 727 3749 727
Size: 3022 1507
BspHI T'CATG_A
Cuts at: 3705 3705
Size: 4529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 548 607 638 1328 1579 1793 2095 2476 548
Size: 59 31 690 251 214 302 381 2601
Fragments arranged by size:
2601 690 381 302 251 214 59 31
BsrBI CCG'CTC
Cuts at: 50 136 351 2821 2875 4359 4524 50
Size: 86 215 2470 54 1484 165 55
Fragments arranged by size:
2470 1484 215 165 86 55 54
BsrDI GCAATG_nn'
Cuts at: 2441 2441
Size: 4529
BsrFI r'CCGG_y
Cuts at: 843 1214 2527 2708 2917 843
Size: 371 1313 181 209 2455
Fragments arranged by size:
2455 1313 371 209 181
BssSI C'ACGA_G
Cuts at: 2800 3158 2800
Size: 358 4171
BstAPI GCAn_nnn'nTGC
Cuts at: 1041 1155 1906 1041
Size: 114 751 3664
Fragments arranged by size:
3664 751 114
BstBI TT'CG_AA
Cuts at: 2890 2890
Size: 4529
BstEII G'GTnAC_C
Cuts at: 846 855 876 846
Size: 9 21 4499
Fragments arranged by size:
4499 21 9
BstXI CCAn_nnnn'nTGG
Cuts at: 1311 1311
Size: 4529
BstYI r'GATC_y
Cuts at: 1 908 1176 1554 2021 2044 2379 2625 3637
Size: 907 268 378 467 23 335 246 1012
Cuts at: 3637 3723 3735 4081 1
Size: 86 12 346 449
Fragments arranged by size:
1012 907 467 449 378 346 335 268
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 330 330
Size: 4529
BtgI C'CryG_G
Cuts at: 851 1055 1349 851
Size: 204 294 4031
Fragments arranged by size:
4031 294 204
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 497 774 1230 2479 2660 2823 497
Size: 277 456 1249 181 163 2203
Fragments arranged by size:
2203 1249 456 277 181 163
BtsI GCAGTG_nn'
Cuts at: 1772 1772
Size: 4529
DraI TTT'AAA
Cuts at: 1528 1528
Size: 4529
DrdI GACnn_nn'nnGTC
Cuts at: 2235 3093 2235
Size: 858 3671
EaeI y'GGCC_r
Cuts at: 149 1352 2114 2288 2679 2706 4051 149
Size: 1203 762 174 391 27 1345 627
Fragments arranged by size:
1345 1203 762 627 391 174 27
EagI C'GGCC_G
Cuts at: 2114 2114
Size: 4529
EarI CTCTTCn'nnn_
Cuts at: 1033 1063 1403 1424 2552 2762 1033
Size: 30 340 21 1128 210 2800
Fragments arranged by size:
2800 1128 340 210 30 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 402 2737 3047 3193 4339 402
Size: 2335 310 146 1146 592
Fragments arranged by size:
2335 1146 592 310 146
EcoICRI GAG'CTC
Cuts at: 1541 1541
Size: 4529
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 462 565 571 760 1471 1540 2354 2786
Size: 134 103 6 189 711 69 814 432
Cuts at: 2786 3533 3801 328
Size: 747 268 1056
Fragments arranged by size:
1056 814 747 711 432 268 189 134
103 69 6
EcoO109I rG'GnC_Cy
Cuts at: 525 652 653 696 1668 525
Size: 127 1 43 972 3386
Fragments arranged by size:
3386 972 127 43 1
EcoRI G'AATT_C
Cuts at: 708 1533 708
Size: 825 3704
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2548 2548
Size: 4529
FspI TGC'GCA
Cuts at: 2310 2310
Size: 4529
HaeII r_GCGC'y
Cuts at: 775 1963 2211 3233 4212 775
Size: 1188 248 1022 979 1092
Fragments arranged by size:
1188 1092 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 925 952 957 984 1191 1223 2371 2401 2403
Size: 27 5 27 207 32 1148 30 2
Cuts at: 2403 2433 925
Size: 30 3021
Fragments arranged by size:
3021 1148 207 32 30 30 27 27
5 2
HincII GTy'rAC
Cuts at: 807 927 1464 1568 807
Size: 120 537 104 3768
Fragments arranged by size:
3768 537 120 104
HpaI GTT'AAC
Cuts at: 927 927
Size: 4529
KasI G'GCGC_C
Cuts at: 1959 2207 1959
Size: 248 4281
KpnI G_GTAC'C
Cuts at: 1549 1549
Size: 4529
MluI A'CGCG_T
Cuts at: 3962 3962
Size: 4529
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3200 3384 3200
Size: 184 4345
MscI TGG'CCA
Cuts at: 151 1354 2290 151
Size: 1203 936 2390
Fragments arranged by size:
2390 1203 936
MslI CAynn'nnrTG
Cuts at: 271 793 1354 2518 2645 271
Size: 522 561 1164 127 2155
Fragments arranged by size:
2155 1164 561 522 127
MspA1I CmG'CkG
Cuts at: 256 306 1640 1954 2314 3327 3572 256
Size: 50 1334 314 360 1013 245 1213
Fragments arranged by size:
1334 1213 1013 360 314 245 50
NaeI GCC'GGC
Cuts at: 2710 2710
Size: 4529
NarI GG'CG_CC
Cuts at: 1960 2208 1960
Size: 248 4281
NcoI C'CATG_G
Cuts at: 851 1349 851
Size: 498 4031
NgoMIV G'CCGG_C
Cuts at: 2708 2708
Size: 4529
NspI r_CATG'y
Cuts at: 121 1003 1582 2082 2989 121
Size: 882 579 500 907 1661
Fragments arranged by size:
1661 907 882 579 500
PasI CC'CwG_GG
Cuts at: 390 747 864 894 1282 390
Size: 357 117 30 388 3637
Fragments arranged by size:
3637 388 357 117 30
PciI A'CATG_T
Cuts at: 2985 2985
Size: 4529
PflMI CCAn_nnn'nTGG
Cuts at: 543 543
Size: 4529
PfoI T'CCnGG_A
Cuts at: 947 947
Size: 4529
PmeI GTTT'AAAC
Cuts at: 1528 1528
Size: 4529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3668 3700 3668
Size: 32 4497
PpuMI rG'GwC_Cy
Cuts at: 525 696 525
Size: 171 4358
PshAI GACnn'nnGTC
Cuts at: 802 1483 802
Size: 681 3848
PspOMI G'GGCC_C
Cuts at: 652 652
Size: 4529
PstI C_TGCA'G
Cuts at: 707 1151 1265 1325 1475 1576 2261 707
Size: 444 114 60 150 101 685 2975
Fragments arranged by size:
2975 685 444 150 114 101 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4529
PvuII CAG'CTG
Cuts at: 256 306 1954 2314 256
Size: 50 1648 360 2471
Fragments arranged by size:
2471 1648 360 50
RsrII CG'GwC_CG
Cuts at: 2724 2724
Size: 4529
SacI G_AGCT'C
Cuts at: 1543 1543
Size: 4529
SalI G'TCGA_C
Cuts at: 1462 1566 1462
Size: 104 4425
SapI GCTCTTCn'nnn_
Cuts at: 1033 2552 2762 1033
Size: 1519 210 2800
Fragments arranged by size:
2800 1519 210
SbfI CC_TGCA'GG
Cuts at: 1576 1576
Size: 4529
ScaI AGT'ACT
Cuts at: 4046 4046
Size: 4529
SfcI C'TryA_G
Cuts at: 33 703 1147 1261 1321 1471 1572 2257 3250
Size: 670 444 114 60 150 101 685 993
Cuts at: 3250 3441 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 670 444 191 150 114
101 60
SfoI GGC'GCC
Cuts at: 1961 2209 1961
Size: 248 4281
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4529
SmaI CCC'GGG
Cuts at: 1551 1551
Size: 4529
SmlI C'TyrA_G
Cuts at: 1684 2599 3091 3353 1684
Size: 915 492 262 2860
Fragments arranged by size:
2860 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4529
SphI G_CATG'C
Cuts at: 1003 1582 2082 1003
Size: 579 500 3450
Fragments arranged by size:
3450 579 500
StyI C'CwwG_G
Cuts at: 221 851 1349 1663 2017 221
Size: 630 498 314 354 2733
Fragments arranged by size:
2733 630 498 354 314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 403 895 1237 2112 2245 2704 2913 403
Size: 492 342 875 133 459 209 2019
Fragments arranged by size:
2019 875 492 459 342 209 133
TatI w'GTAC_w
Cuts at: 4044 4044
Size: 4529
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 683 715 1498 1530 4046 4078 683
Size: 32 783 32 2516 32 1134
Fragments arranged by size:
2516 1134 783 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2326 2326
Size: 4529
XbaI T'CTAG_A
Cuts at: 1560 1560
Size: 4529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 423 2024 423
Size: 1601 2928
XmaI C'CCGG_G
Cuts at: 1549 1549
Size: 4529
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BbsI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI