Restriction map for
pF1KB8288
(Circular) (Six-base) MAPSORT of: pF1KB8288.seq Check: 4163 from: 1 to: 4334
pF1KB8288 4334 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1372 1372
Size: 4334
Acc65I G'GTAC_C
Cuts at: 1350 1350
Size: 4334
AclI AA'CG_TT
Cuts at: 4174 4174
Size: 4334
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 2159 2591 3338 3606 400
Size: 1759 432 747 268 1128
Fragments arranged by size:
1759 1128 747 432 268
AflIII A'CryG_T
Cuts at: 1686 2790 3767 1686
Size: 1104 977 2253
Fragments arranged by size:
2253 1104 977
AgeI A'CCGG_T
Cuts at: 2722 2722
Size: 4334
AhdI GACnn_n'nnGTC
Cuts at: 1136 1136
Size: 4334
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 310 310
Size: 4334
AlwNI CAG_nnn'CTG
Cuts at: 138 150 440 727 1639 3206 138
Size: 12 290 287 912 1567 1266
Fragments arranged by size:
1567 1266 912 290 287 12
ApaLI G'TGCA_C
Cuts at: 3104 3104
Size: 4334
ApoI r'AATT_y
Cuts at: 202 1338 3687 3943 202
Size: 1136 2349 256 593
Fragments arranged by size:
2349 1136 593 256
AseI AT'TA_AT
Cuts at: 20 551 20
Size: 531 3803
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
AvaI C'yCGr_G
Cuts at: 1074 1354 1074
Size: 280 4054
BamHI G'GATC_C
Cuts at: 387 1078 1359 387
Size: 691 281 3362
Fragments arranged by size:
3362 691 281
BanI G'GyrC_C
Cuts at: 1030 1350 1764 2012 2047 1030
Size: 320 414 248 35 3317
Fragments arranged by size:
3317 414 320 248 35
BanII G_rGCy'C
Cuts at: 294 926 946 1348 2378 294
Size: 632 20 402 1030 2250
Fragments arranged by size:
2250 1030 632 402 20
BbeI G_GCGC'C
Cuts at: 1768 2016 1768
Size: 248 4086
BbsI GAAGACnn'nnnn_
Cuts at: 626 626
Size: 4334
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1020 1054 1125 1159 1020
Size: 34 71 34 4195
Fragments arranged by size:
4195 71 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2232 2993 4334 2232
Size: 761 1341 2232
Fragments arranged by size:
2232 1341 761
BclI T'GATC_A
Cuts at: 653 1854 653
Size: 1201 3133
BglI GCCn_nnn'nGGC
Cuts at: 583 748 583
Size: 165 4169
BglII A'GATC_T
Cuts at: 770 1849 3886 770
Size: 1079 2037 1218
Fragments arranged by size:
2037 1218 1079
BlpI GC'TnA_GC
Cuts at: 283 1446 283
Size: 1163 3171
Bme1580I G_kGCm'C
Cuts at: 1959 2052 3108 1959
Size: 93 1056 3185
Fragments arranged by size:
3185 1056 93
BmgBI CAC'GTC
Cuts at: 687 719 687
Size: 32 4302
BmrI ACTGGGnnnn_n'
Cuts at: 1268 1546 1627 1962 4106 1268
Size: 278 81 335 2144 1496
Fragments arranged by size:
2144 1496 335 278 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2701 2701
Size: 4334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 910 940 1134 1185 1280 625
Size: 285 30 194 51 95 3679
Fragments arranged by size:
3679 285 194 95 51 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1510 2389 2881 3179 1510
Size: 879 492 298 2665
Fragments arranged by size:
2665 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4038 4038
Size: 4334
BsaAI yAC'GTr
Cuts at: 1687 1687
Size: 4334
BsaBI GATnn'nnATC
Cuts at: 6 1848 3885 6
Size: 1842 2037 455
Fragments arranged by size:
2037 1842 455
BsaHI Gr'CG_yC
Cuts at: 448 575 736 1765 2013 2715 4211 448
Size: 127 161 1029 248 702 1496 571
Fragments arranged by size:
1496 1029 702 571 248 161 127
BsaWI w'CCGG_w
Cuts at: 453 1523 2044 2722 2996 3143 3554 453
Size: 1070 521 678 274 147 411 1233
Fragments arranged by size:
1233 1070 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 942 972 1027 1057 942
Size: 30 55 30 4219
Fragments arranged by size:
4219 55 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 622 765 202
Size: 420 143 3771
Fragments arranged by size:
3771 420 143
BseYI C'CCAG_C
Cuts at: 1062 1089 1759 2517 3094 3590 1062
Size: 27 670 758 577 496 1806
Fragments arranged by size:
1806 758 670 577 496 27
BsiEI CG_ry'CG
Cuts at: 104 1876 1922 3130 104
Size: 1772 46 1208 1308
Fragments arranged by size:
1772 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1025 1348 2126 2316 3108 1025
Size: 323 778 190 792 2251
Fragments arranged by size:
2251 792 778 323 190
BsmBI CGTCTCn'nnnn_
Cuts at: 455 765 455
Size: 310 4024
Bsp1286I G_dGCh'C
Cuts at: 294 926 946 1025 1348 1959 2052 2126 2316
Size: 632 20 79 323 611 93 74 190
Cuts at: 2316 2378 3108 294
Size: 62 730 1520
Fragments arranged by size:
1520 730 632 611 323 190 93 79
74 62 20
BspEI T'CCGG_A
Cuts at: 453 3554 453
Size: 3101 1233
BspHI T'CATG_A
Cuts at: 3510 3510
Size: 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1384 1598 1900 2281 1384
Size: 214 302 381 3437
Fragments arranged by size:
3437 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2626 2680 4164 4329 50
Size: 2576 54 1484 165 55
Fragments arranged by size:
2576 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2246 2246
Size: 4334
BsrFI r'CCGG_y
Cuts at: 696 2332 2513 2722 696
Size: 1636 181 209 2308
Fragments arranged by size:
2308 1636 209 181
BsrGI T'GTAC_A
Cuts at: 350 350
Size: 4334
BssSI C'ACGA_G
Cuts at: 501 2605 2963 501
Size: 2104 358 1872
Fragments arranged by size:
2104 1872 358
BstAPI GCAn_nnn'nTGC
Cuts at: 150 1711 150
Size: 1561 2773
BstBI TT'CG_AA
Cuts at: 2695 2695
Size: 4334
BstXI CCAn_nnnn'nTGG
Cuts at: 699 996 699
Size: 297 4037
BstYI r'GATC_y
Cuts at: 1 374 387 770 1078 1359 1826 1849 2184
Size: 373 13 383 308 281 467 23 335
Cuts at: 2184 2430 3442 3528 3540 3886 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 383 373 346 335 308
281 246 86 23 13 12
BtgI C'CryG_G
Cuts at: 107 632 107
Size: 525 3809
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1032 2284 2465 2628 1032
Size: 1252 181 163 2738
Fragments arranged by size:
2738 1252 181 163
BtsI GCAGTG_nn'
Cuts at: 1141 1240 1577 1141
Size: 99 337 3898
Fragments arranged by size:
3898 337 99
DraI TTT'AAA
Cuts at: 1333 1333
Size: 4334
DraIII CAC_nnn'GTG
Cuts at: 128 399 128
Size: 271 4063
DrdI GACnn_nn'nnGTC
Cuts at: 685 2040 2898 685
Size: 1355 858 2121
Fragments arranged by size:
2121 1355 858
EaeI y'GGCC_r
Cuts at: 230 485 764 1251 1919 2093 2484 2511 3856
Size: 255 279 487 668 174 391 27 1345
Cuts at: 3856 230
Size: 708
Fragments arranged by size:
1345 708 668 487 391 279 255 174
27
EagI C'GGCC_G
Cuts at: 1919 1919
Size: 4334
EarI CTCTTCn'nnn_
Cuts at: 889 971 2357 2567 889
Size: 82 1386 210 2656
Fragments arranged by size:
2656 1386 210 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 729 801 2542 2852 2998 4144 729
Size: 72 1741 310 146 1146 919
Fragments arranged by size:
1741 1146 919 310 146 72
EcoICRI GAG'CTC
Cuts at: 1346 1346
Size: 4334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 625 910 940 1134 1185 1280 2159 2591
Size: 225 285 30 194 51 95 879 432
Cuts at: 2591 3338 3606 400
Size: 747 268 1128
Fragments arranged by size:
1128 879 747 432 285 268 225 194
95 51 30
EcoO109I rG'GnC_Cy
Cuts at: 1473 1473
Size: 4334
EcoRI G'AATT_C
Cuts at: 202 1338 202
Size: 1136 3198
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2353 2353
Size: 4334
FspI TGC'GCA
Cuts at: 2115 2115
Size: 4334
HaeII r_GCGC'y
Cuts at: 1012 1768 2016 3038 4017 1012
Size: 756 248 1022 979 1329
Fragments arranged by size:
1329 1022 979 756 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 375 407 942 974 1027 1059 1122 1154 2176
Size: 32 535 32 53 32 63 32 1022
Cuts at: 2176 2206 2208 2238 375
Size: 30 2 30 2471
Fragments arranged by size:
2471 1022 535 63 53 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1373 1373
Size: 4334
KasI G'GCGC_C
Cuts at: 1764 2012 1764
Size: 248 4086
KpnI G_GTAC'C
Cuts at: 1354 1354
Size: 4334
MluI A'CGCG_T
Cuts at: 3767 3767
Size: 4334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3005 3189 3005
Size: 184 4150
MscI TGG'CCA
Cuts at: 232 1253 2095 232
Size: 1021 842 2471
Fragments arranged by size:
2471 1021 842
MslI CAynn'nnrTG
Cuts at: 1261 2323 2450 1261
Size: 1062 127 3145
Fragments arranged by size:
3145 1062 127
MspA1I CmG'CkG
Cuts at: 1017 1191 1445 1759 2119 3132 3377 1017
Size: 174 254 314 360 1013 245 1974
Fragments arranged by size:
1974 1013 360 314 254 245 174
NaeI GCC'GGC
Cuts at: 2515 2515
Size: 4334
NarI GG'CG_CC
Cuts at: 1765 2013 1765
Size: 248 4086
NcoI C'CATG_G
Cuts at: 107 107
Size: 4334
NgoMIV G'CCGG_C
Cuts at: 2513 2513
Size: 4334
NspI r_CATG'y
Cuts at: 1260 1387 1887 2794 1260
Size: 127 500 907 2800
Fragments arranged by size:
2800 907 500 127
PciI A'CATG_T
Cuts at: 2790 2790
Size: 4334
PflMI CCAn_nnn'nTGG
Cuts at: 239 239
Size: 4334
PfoI T'CCnGG_A
Cuts at: 368 887 368
Size: 519 3815
PmeI GTTT'AAAC
Cuts at: 1333 1333
Size: 4334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 4302
PstI C_TGCA'G
Cuts at: 431 563 1381 2066 431
Size: 132 818 685 2699
Fragments arranged by size:
2699 818 685 132
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4334
PvuII CAG'CTG
Cuts at: 1759 2119 1759
Size: 360 3974
RsrII CG'GwC_CG
Cuts at: 2529 2529
Size: 4334
SacI G_AGCT'C
Cuts at: 1348 1348
Size: 4334
SalI G'TCGA_C
Cuts at: 1371 1371
Size: 4334
SapI GCTCTTCn'nnn_
Cuts at: 2357 2567 2357
Size: 210 4124
SbfI CC_TGCA'GG
Cuts at: 1381 1381
Size: 4334
ScaI AGT'ACT
Cuts at: 1301 3851 1301
Size: 2550 1784
SfcI C'TryA_G
Cuts at: 33 427 559 1377 2062 3055 3246 33
Size: 394 132 818 685 993 191 1121
Fragments arranged by size:
1121 993 818 685 394 191 132
SfoI GGC'GCC
Cuts at: 1766 2014 1766
Size: 248 4086
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
SmaI CCC'GGG
Cuts at: 1356 1356
Size: 4334
SmlI C'TyrA_G
Cuts at: 1489 2404 2896 3158 1489
Size: 915 492 262 2665
Fragments arranged by size:
2665 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4334
SphI G_CATG'C
Cuts at: 1387 1887 1387
Size: 500 3834
StuI AGG'CCT
Cuts at: 530 530
Size: 4334
StyI C'CwwG_G
Cuts at: 107 1468 1822 107
Size: 1361 354 2619
Fragments arranged by size:
2619 1361 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 821 1917 2050 2509 2718 821
Size: 1096 133 459 209 2437
Fragments arranged by size:
2437 1096 459 209 133
TatI w'GTAC_w
Cuts at: 350 533 1299 3849 350
Size: 183 766 2550 835
Fragments arranged by size:
2550 835 766 183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3851 3883 3851
Size: 32 4302
Tth111I GACn'n_nGTC
Cuts at: 954 2131 954
Size: 1177 3157
XbaI T'CTAG_A
Cuts at: 1365 1365
Size: 4334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1829 1829
Size: 4334
XmaI C'CCGG_G
Cuts at: 1354 1354
Size: 4334
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BmtI Bpu10I BsgI BsiWI
BsmI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI