Restriction map for
pF1KB8286
(Circular) (Six-base) MAPSORT of: pF1KB8286.seq Check: 1561 from: 1 to: 4310
pF1KB8286 4310 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1348 1348
Size: 4310
Acc65I G'GTAC_C
Cuts at: 1326 1326
Size: 4310
AclI AA'CG_TT
Cuts at: 591 4150 591
Size: 3559 751
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 1046 2135 2567 3314 3582 448
Size: 598 1089 432 747 268 1176
Fragments arranged by size:
1176 1089 747 598 432 268
AflII C'TTAA_G
Cuts at: 1050 1050
Size: 4310
AflIII A'CryG_T
Cuts at: 505 1662 2766 3743 505
Size: 1157 1104 977 1072
Fragments arranged by size:
1157 1104 1072 977
AgeI A'CCGG_T
Cuts at: 2698 2698
Size: 4310
AlwNI CAG_nnn'CTG
Cuts at: 426 943 1615 3182 426
Size: 517 672 1567 1554
Fragments arranged by size:
1567 1554 672 517
ApaLI G'TGCA_C
Cuts at: 3080 3080
Size: 4310
ApoI r'AATT_y
Cuts at: 754 877 1314 3663 3919 754
Size: 123 437 2349 256 1145
Fragments arranged by size:
2349 1145 437 256 123
AseI AT'TA_AT
Cuts at: 20 20
Size: 4310
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
AvaI C'yCGr_G
Cuts at: 514 1154 1330 514
Size: 640 176 3494
Fragments arranged by size:
3494 640 176
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1027 1060 1027
Size: 33 4277
BamHI G'GATC_C
Cuts at: 1335 1335
Size: 4310
BanI G'GyrC_C
Cuts at: 171 1326 1740 1988 2023 171
Size: 1155 414 248 35 2458
Fragments arranged by size:
2458 1155 414 248 35
BanII G_rGCy'C
Cuts at: 1324 2354 1324
Size: 1030 3280
BbeI G_GCGC'C
Cuts at: 1744 1992 1744
Size: 248 4062
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4276
BciVI GTATCCnnnnn_n'
Cuts at: 2208 2969 4310 2208
Size: 761 1341 2208
Fragments arranged by size:
2208 1341 761
BclI T'GATC_A
Cuts at: 1830 1830
Size: 4310
BglII A'GATC_T
Cuts at: 1825 3862 1825
Size: 2037 2273
BlpI GC'TnA_GC
Cuts at: 1422 1422
Size: 4310
Bme1580I G_kGCm'C
Cuts at: 1935 2028 3084 1935
Size: 93 1056 3161
Fragments arranged by size:
3161 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1522 1603 1938 4082 1522
Size: 81 335 2144 1750
Fragments arranged by size:
2144 1750 335 81
BmtI G_CTAG'C
Cuts at: 458 476 458
Size: 18 4292
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2677 2677
Size: 4310
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 410
Size: 4310
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1245 1486 2365 2857 3155 1245
Size: 241 879 492 298 2400
Fragments arranged by size:
2400 879 492 298 241
BsaI GGTCTCn'nnnn_
Cuts at: 4014 4014
Size: 4310
BsaAI yAC'GTr
Cuts at: 508 1663 508
Size: 1155 3155
BsaBI GATnn'nnATC
Cuts at: 6 1824 3861 6
Size: 1818 2037 455
Fragments arranged by size:
2037 1818 455
BsaHI Gr'CG_yC
Cuts at: 1741 1989 2691 4187 1741
Size: 248 702 1496 1864
Fragments arranged by size:
1864 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1499 2020 2698 2972 3119 3530 1499
Size: 521 678 274 147 411 2279
Fragments arranged by size:
2279 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 495 525 495
Size: 30 4280
BseYI C'CCAG_C
Cuts at: 1735 2493 3070 3566 1735
Size: 758 577 496 2479
Fragments arranged by size:
2479 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1852 1898 3106 104
Size: 1748 46 1208 1308
Fragments arranged by size:
1748 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 853 1324 2102 2292 3084 853
Size: 471 778 190 792 2079
Fragments arranged by size:
2079 792 778 471 190
Bsp1286I G_dGCh'C
Cuts at: 853 1324 1935 2028 2102 2292 2354 3084 853
Size: 471 611 93 74 190 62 730 2079
Fragments arranged by size:
2079 730 611 471 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3530 3530
Size: 4310
BspHI T'CATG_A
Cuts at: 3486 3486
Size: 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1360 1574 1876 2257 1360
Size: 214 302 381 3413
Fragments arranged by size:
3413 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2602 2656 4140 4305 50
Size: 2552 54 1484 165 55
Fragments arranged by size:
2552 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2222 2222
Size: 4310
BsrFI r'CCGG_y
Cuts at: 769 2308 2489 2698 769
Size: 1539 181 209 2381
Fragments arranged by size:
2381 1539 209 181
BssSI C'ACGA_G
Cuts at: 494 2581 2939 494
Size: 2087 358 1865
Fragments arranged by size:
2087 1865 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1687 1687
Size: 4310
BstBI TT'CG_AA
Cuts at: 2671 2671
Size: 4310
BstYI r'GATC_y
Cuts at: 1 537 1335 1802 1825 2160 2406 3418 3504
Size: 536 798 467 23 335 246 1012 86
Cuts at: 3504 3516 3862 1
Size: 12 346 449
Fragments arranged by size:
1012 798 536 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 123 123
Size: 4310
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2260 2441 2604 2260
Size: 181 163 3966
Fragments arranged by size:
3966 181 163
BtsI GCAGTG_nn'
Cuts at: 1553 1553
Size: 4310
DraI TTT'AAA
Cuts at: 825 1184 1235 1309 825
Size: 359 51 74 3826
Fragments arranged by size:
3826 359 74 51
DrdI GACnn_nn'nnGTC
Cuts at: 2016 2874 2016
Size: 858 3452
EaeI y'GGCC_r
Cuts at: 1895 2069 2460 2487 3832 1895
Size: 174 391 27 1345 2373
Fragments arranged by size:
2373 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1895 1895
Size: 4310
EarI CTCTTCn'nnn_
Cuts at: 1025 1250 2333 2543 1025
Size: 225 1083 210 2792
Fragments arranged by size:
2792 1083 225 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2518 2828 2974 4120 2518
Size: 310 146 1146 2708
Fragments arranged by size:
2708 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1322 1322
Size: 4310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 448 1046 2135 2567 3314 3582 410
Size: 38 598 1089 432 747 268 1138
Fragments arranged by size:
1138 1089 747 598 432 268 38
EcoO109I rG'GnC_Cy
Cuts at: 327 423 1449 327
Size: 96 1026 3188
Fragments arranged by size:
3188 1026 96
EcoRI G'AATT_C
Cuts at: 877 1314 877
Size: 437 3873
EcoRV GAT'ATC
Cuts at: 490 490
Size: 4310
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1065 2329 1065
Size: 1264 3046
FspI TGC'GCA
Cuts at: 2091 2091
Size: 4310
HaeII r_GCGC'y
Cuts at: 1744 1992 3014 3993 1744
Size: 248 1022 979 2061
Fragments arranged by size:
2061 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 918 932 950 964 2152 2182 2184 2214 918
Size: 14 18 14 1188 30 2 30 3014
Fragments arranged by size:
3014 1188 30 30 18 14 14 2
HincII GTy'rAC
Cuts at: 698 890 1349 698
Size: 192 459 3659
Fragments arranged by size:
3659 459 192
KasI G'GCGC_C
Cuts at: 1740 1988 1740
Size: 248 4062
KpnI G_GTAC'C
Cuts at: 1330 1330
Size: 4310
MluI A'CGCG_T
Cuts at: 3743 3743
Size: 4310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 205 645 2981 3165 205
Size: 440 2336 184 1350
Fragments arranged by size:
2336 1350 440 184
MscI TGG'CCA
Cuts at: 2071 2071
Size: 4310
MslI CAynn'nnrTG
Cuts at: 2299 2426 2299
Size: 127 4183
MspA1I CmG'CkG
Cuts at: 1421 1735 2095 3108 3353 1421
Size: 314 360 1013 245 2378
Fragments arranged by size:
2378 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2491 2491
Size: 4310
NarI GG'CG_CC
Cuts at: 1741 1989 1741
Size: 248 4062
NdeI CA'TA_TG
Cuts at: 191 191
Size: 4310
NgoMIV G'CCGG_C
Cuts at: 2489 2489
Size: 4310
NheI G'CTAG_C
Cuts at: 454 472 454
Size: 18 4292
NspI r_CATG'y
Cuts at: 1363 1863 2770 1363
Size: 500 907 2903
Fragments arranged by size:
2903 907 500
PciI A'CATG_T
Cuts at: 2766 2766
Size: 4310
PfoI T'CCnGG_A
Cuts at: 936 936
Size: 4310
PmeI GTTT'AAAC
Cuts at: 1309 1309
Size: 4310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 3449
Size: 32 4278
PpuMI rG'GwC_Cy
Cuts at: 327 423 327
Size: 96 4214
PstI C_TGCA'G
Cuts at: 1357 2042 1357
Size: 685 3625
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4310
PvuII CAG'CTG
Cuts at: 1735 2095 1735
Size: 360 3950
RsrII CG'GwC_CG
Cuts at: 2505 2505
Size: 4310
SacI G_AGCT'C
Cuts at: 1324 1324
Size: 4310
SalI G'TCGA_C
Cuts at: 1347 1347
Size: 4310
SapI GCTCTTCn'nnn_
Cuts at: 2333 2543 2333
Size: 210 4100
SbfI CC_TGCA'GG
Cuts at: 1357 1357
Size: 4310
ScaI AGT'ACT
Cuts at: 1179 1230 3827 1179
Size: 51 2597 1662
Fragments arranged by size:
2597 1662 51
SfcI C'TryA_G
Cuts at: 33 1353 2038 3031 3222 33
Size: 1320 685 993 191 1121
Fragments arranged by size:
1320 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1742 1990 1742
Size: 248 4062
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
SmaI CCC'GGG
Cuts at: 1332 1332
Size: 4310
SmlI C'TyrA_G
Cuts at: 1050 1224 1465 2380 2872 3134 1050
Size: 174 241 915 492 262 2226
Fragments arranged by size:
2226 915 492 262 241 174
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4310
SphI G_CATG'C
Cuts at: 1363 1863 1363
Size: 500 3810
SspI AAT'ATT
Cuts at: 582 582
Size: 4310
StyI C'CwwG_G
Cuts at: 1444 1798 1444
Size: 354 3956
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 602 1893 2026 2485 2694 602
Size: 1291 133 459 209 2218
Fragments arranged by size:
2218 1291 459 209 133
TatI w'GTAC_w
Cuts at: 1021 1177 1228 3825 1021
Size: 156 51 2597 1506
Fragments arranged by size:
2597 1506 156 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3827 3859 3827
Size: 32 4278
Tth111I GACn'n_nGTC
Cuts at: 2107 2107
Size: 4310
XbaI T'CTAG_A
Cuts at: 1341 1341
Size: 4310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 182 1805 182
Size: 1623 2687
XmaI C'CCGG_G
Cuts at: 1330 1330
Size: 4310
XmnI GAAnn'nnTTC
Cuts at: 433 433
Size: 4310
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NheI
NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BbsI BbvCI BfrBI BglI BmgBI Bpu10I
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I BtgI ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NcoI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI