Restriction map for
pF1KB8280
(Circular) (Six-base) MAPSORT of: pF1KB8280.seq Check: 1490 from: 1 to: 3683
pF1KB8280 3683 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AatII G_ACGT'C
Cuts at: 549 549
Size: 3683
AccI GT'mk_AC
Cuts at: 721 721
Size: 3683
Acc65I G'GTAC_C
Cuts at: 659 699 659
Size: 40 3643
AclI AA'CG_TT
Cuts at: 3523 3523
Size: 3683
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 1508 1940 2687 2955 628
Size: 880 432 747 268 1356
Fragments arranged by size:
1356 880 747 432 268
AflIII A'CryG_T
Cuts at: 1035 2139 3116 1035
Size: 1104 977 1602
Fragments arranged by size:
1602 1104 977
AgeI A'CCGG_T
Cuts at: 2071 2071
Size: 3683
AlwNI CAG_nnn'CTG
Cuts at: 493 988 2555 493
Size: 495 1567 1621
Fragments arranged by size:
1621 1567 495
ApaLI G'TGCA_C
Cuts at: 2453 2453
Size: 3683
ApoI r'AATT_y
Cuts at: 141 687 3036 3292 141
Size: 546 2349 256 532
Fragments arranged by size:
2349 546 532 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3683
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3683
AvaI C'yCGr_G
Cuts at: 167 187 703 167
Size: 20 516 3147
Fragments arranged by size:
3147 516 20
BamHI G'GATC_C
Cuts at: 708 708
Size: 3683
BanI G'GyrC_C
Cuts at: 659 699 1113 1361 1396 659
Size: 40 414 248 35 2946
Fragments arranged by size:
2946 414 248 40 35
BanII G_rGCy'C
Cuts at: 697 1727 697
Size: 1030 2653
BbeI G_GCGC'C
Cuts at: 1117 1365 1117
Size: 248 3435
BbsI GAAGACnn'nnnn_
Cuts at: 336 570 336
Size: 234 3449
BciVI GTATCCnnnnn_n'
Cuts at: 514 1581 2342 3683 514
Size: 1067 761 1341 514
Fragments arranged by size:
1341 1067 761 514
BclI T'GATC_A
Cuts at: 1203 1203
Size: 3683
BglII A'GATC_T
Cuts at: 191 1198 3235 191
Size: 1007 2037 639
Fragments arranged by size:
2037 1007 639
BlpI GC'TnA_GC
Cuts at: 795 795
Size: 3683
Bme1580I G_kGCm'C
Cuts at: 1308 1401 2457 1308
Size: 93 1056 2534
Fragments arranged by size:
2534 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 895 976 1311 3455 895
Size: 81 335 2144 1123
Fragments arranged by size:
2144 1123 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2050 2050
Size: 3683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 254
Size: 3683
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 1738 2230 2528 859
Size: 879 492 298 2014
Fragments arranged by size:
2014 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3387 3387
Size: 3683
BsaAI yAC'GTr
Cuts at: 1036 1036
Size: 3683
BsaBI GATnn'nnATC
Cuts at: 6 1197 3234 6
Size: 1191 2037 455
Fragments arranged by size:
2037 1191 455
BsaHI Gr'CG_yC
Cuts at: 546 1114 1362 2064 3560 546
Size: 568 248 702 1496 669
Fragments arranged by size:
1496 702 669 568 248
BsaWI w'CCGG_w
Cuts at: 872 1393 2071 2345 2492 2903 872
Size: 521 678 274 147 411 1652
Fragments arranged by size:
1652 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 426 456 426
Size: 30 3653
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 415 415
Size: 3683
BseYI C'CCAG_C
Cuts at: 1108 1866 2443 2939 1108
Size: 758 577 496 1852
Fragments arranged by size:
1852 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 556
Size: 3683
BsiEI CG_ry'CG
Cuts at: 104 1225 1271 2479 104
Size: 1121 46 1208 1308
Fragments arranged by size:
1308 1208 1121 46
BsiHKAI G_wGCw'C
Cuts at: 697 1475 1665 2457 697
Size: 778 190 792 1923
Fragments arranged by size:
1923 792 778 190
BsmI GAATG_Cn'
Cuts at: 348 571 618 348
Size: 223 47 3413
Fragments arranged by size:
3413 223 47
BsmBI CGTCTCn'nnnn_
Cuts at: 413 553 413
Size: 140 3543
Bsp1286I G_dGCh'C
Cuts at: 697 1308 1401 1475 1665 1727 2457 697
Size: 611 93 74 190 62 730 1923
Fragments arranged by size:
1923 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2903 2903
Size: 3683
BspHI T'CATG_A
Cuts at: 2859 2859
Size: 3683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 733 947 1249 1630 733
Size: 214 302 381 2786
Fragments arranged by size:
2786 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1975 2029 3513 3678 50
Size: 1925 54 1484 165 55
Fragments arranged by size:
1925 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1595 1595
Size: 3683
BsrFI r'CCGG_y
Cuts at: 1681 1862 2071 1681
Size: 181 209 3293
Fragments arranged by size:
3293 209 181
BssSI C'ACGA_G
Cuts at: 220 1954 2312 220
Size: 1734 358 1591
Fragments arranged by size:
1734 1591 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1060 1060
Size: 3683
BstBI TT'CG_AA
Cuts at: 2044 2044
Size: 3683
BstXI CCAn_nnnn'nTGG
Cuts at: 513 513
Size: 3683
BstYI r'GATC_y
Cuts at: 1 191 708 1175 1198 1533 1779 2791 2877
Size: 190 517 467 23 335 246 1012 86
Cuts at: 2877 2889 3235 1
Size: 12 346 449
Fragments arranged by size:
1012 517 467 449 346 335 246 190
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 478 486 478
Size: 8 3675
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1633 1814 1977 1633
Size: 181 163 3339
Fragments arranged by size:
3339 181 163
BtsI GCAGTG_nn'
Cuts at: 396 926 396
Size: 530 3153
DraI TTT'AAA
Cuts at: 682 682
Size: 3683
DrdI GACnn_nn'nnGTC
Cuts at: 1389 2247 1389
Size: 858 2825
EaeI y'GGCC_r
Cuts at: 570 1268 1442 1833 1860 3205 570
Size: 698 174 391 27 1345 1048
Fragments arranged by size:
1345 1048 698 391 174 27
EagI C'GGCC_G
Cuts at: 1268 1268
Size: 3683
EarI CTCTTCn'nnn_
Cuts at: 1706 1916 1706
Size: 210 3473
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1891 2201 2347 3493 1891
Size: 310 146 1146 2081
Fragments arranged by size:
2081 1146 310 146
EcoICRI GAG'CTC
Cuts at: 695 695
Size: 3683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 628 1508 1940 2687 2955 254
Size: 374 880 432 747 268 982
Fragments arranged by size:
982 880 747 432 374 268
EcoO109I rG'GnC_Cy
Cuts at: 482 822 482
Size: 340 3343
EcoRI G'AATT_C
Cuts at: 687 687
Size: 3683
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1702 1702
Size: 3683
FspI TGC'GCA
Cuts at: 1464 1464
Size: 3683
HaeII r_GCGC'y
Cuts at: 470 1117 1365 2387 3366 470
Size: 647 248 1022 979 787
Fragments arranged by size:
1022 979 787 647 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 391 423 508 536 540 568 1525 1555 1557
Size: 32 85 28 4 28 957 30 2
Cuts at: 1557 1587 391
Size: 30 2487
Fragments arranged by size:
2487 957 85 32 30 30 28 28
4 2
HincII GTy'rAC
Cuts at: 318 722 318
Size: 404 3279
KasI G'GCGC_C
Cuts at: 1113 1361 1113
Size: 248 3435
KpnI G_GTAC'C
Cuts at: 663 703 663
Size: 40 3643
MluI A'CGCG_T
Cuts at: 3116 3116
Size: 3683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2354 2538 2354
Size: 184 3499
MscI TGG'CCA
Cuts at: 1444 1444
Size: 3683
MslI CAynn'nnrTG
Cuts at: 511 1672 1799 511
Size: 1161 127 2395
Fragments arranged by size:
2395 1161 127
MspA1I CmG'CkG
Cuts at: 377 794 1108 1468 2481 2726 377
Size: 417 314 360 1013 245 1334
Fragments arranged by size:
1334 1013 417 360 314 245
NaeI GCC'GGC
Cuts at: 1864 1864
Size: 3683
NarI GG'CG_CC
Cuts at: 1114 1362 1114
Size: 248 3435
NgoMIV G'CCGG_C
Cuts at: 1862 1862
Size: 3683
NspI r_CATG'y
Cuts at: 736 1236 2143 736
Size: 500 907 2276
Fragments arranged by size:
2276 907 500
PciI A'CATG_T
Cuts at: 2139 2139
Size: 3683
PmeI GTTT'AAAC
Cuts at: 682 682
Size: 3683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2822 2854 2822
Size: 32 3651
PstI C_TGCA'G
Cuts at: 730 1415 730
Size: 685 2998
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3683
PvuII CAG'CTG
Cuts at: 377 1108 1468 377
Size: 731 360 2592
Fragments arranged by size:
2592 731 360
RsrII CG'GwC_CG
Cuts at: 1878 1878
Size: 3683
SacI G_AGCT'C
Cuts at: 697 697
Size: 3683
SalI G'TCGA_C
Cuts at: 720 720
Size: 3683
SapI GCTCTTCn'nnn_
Cuts at: 1706 1916 1706
Size: 210 3473
SbfI CC_TGCA'GG
Cuts at: 730 730
Size: 3683
ScaI AGT'ACT
Cuts at: 3200 3200
Size: 3683
SfcI C'TryA_G
Cuts at: 33 470 582 726 1411 2404 2595 33
Size: 437 112 144 685 993 191 1121
Fragments arranged by size:
1121 993 685 437 191 144 112
SfoI GGC'GCC
Cuts at: 1115 1363 1115
Size: 248 3435
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3683
SmaI CCC'GGG
Cuts at: 705 705
Size: 3683
SmlI C'TyrA_G
Cuts at: 838 1753 2245 2507 838
Size: 915 492 262 2014
Fragments arranged by size:
2014 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3683
SphI G_CATG'C
Cuts at: 736 1236 736
Size: 500 3183
StuI AGG'CCT
Cuts at: 476 476
Size: 3683
StyI C'CwwG_G
Cuts at: 197 334 817 1171 197
Size: 137 483 354 2709
Fragments arranged by size:
2709 483 354 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1266 1399 1858 2067 1266
Size: 133 459 209 2882
Fragments arranged by size:
2882 459 209 133
TatI w'GTAC_w
Cuts at: 112 3198 112
Size: 3086 597
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3200 3232 3200
Size: 32 3651
Tth111I GACn'n_nGTC
Cuts at: 1480 1480
Size: 3683
XbaI T'CTAG_A
Cuts at: 145 714 145
Size: 569 3114
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 219 1178 219
Size: 959 2724
XmaI C'CCGG_G
Cuts at: 703 703
Size: 3683
ZraI GAC'GTC
Cuts at: 547 547
Size: 3683
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BsrGI BssHII BstEII BstZ17I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI