Restriction map for
pF1KB8278
(Circular) (Six-base) MAPSORT of: pF1KB8278.seq Check: 7001 from: 1 to: 4091
pF1KB8278 4091 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 210 344 1129 210
Size: 134 785 3172
Fragments arranged by size:
3172 785 134
Acc65I G'GTAC_C
Cuts at: 1107 1107
Size: 4091
AclI AA'CG_TT
Cuts at: 3931 3931
Size: 4091
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1916 2348 3095 3363 1916
Size: 432 747 268 2644
Fragments arranged by size:
2644 747 432 268
AflII C'TTAA_G
Cuts at: 630 630
Size: 4091
AflIII A'CryG_T
Cuts at: 229 1443 2547 3524 229
Size: 1214 1104 977 796
Fragments arranged by size:
1214 1104 977 796
AgeI A'CCGG_T
Cuts at: 2479 2479
Size: 4091
AlwNI CAG_nnn'CTG
Cuts at: 1396 2963 1396
Size: 1567 2524
ApaLI G'TGCA_C
Cuts at: 2861 2861
Size: 4091
ApoI r'AATT_y
Cuts at: 514 1095 3444 3700 514
Size: 581 2349 256 905
Fragments arranged by size:
2349 905 581 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4091
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
AvaI C'yCGr_G
Cuts at: 1111 1111
Size: 4091
BamHI G'GATC_C
Cuts at: 1116 1116
Size: 4091
BanI G'GyrC_C
Cuts at: 1107 1521 1769 1804 1107
Size: 414 248 35 3394
Fragments arranged by size:
3394 414 248 35
BanII G_rGCy'C
Cuts at: 1105 2135 1105
Size: 1030 3061
BbeI G_GCGC'C
Cuts at: 1525 1773 1525
Size: 248 3843
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4057
BciVI GTATCCnnnnn_n'
Cuts at: 1989 2750 4091 1989
Size: 761 1341 1989
Fragments arranged by size:
1989 1341 761
BclI T'GATC_A
Cuts at: 1611 1611
Size: 4091
BfrBI ATG'CAT
Cuts at: 111 111
Size: 4091
BglII A'GATC_T
Cuts at: 1606 3643 1606
Size: 2037 2054
BlpI GC'TnA_GC
Cuts at: 1203 1203
Size: 4091
Bme1580I G_kGCm'C
Cuts at: 1716 1809 2865 1716
Size: 93 1056 2942
Fragments arranged by size:
2942 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 926 1303 1384 1719 3863 926
Size: 377 81 335 2144 1154
Fragments arranged by size:
2144 1154 377 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 4091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 771 866 923 268
Size: 503 95 57 3436
Fragments arranged by size:
3436 503 95 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1267 2146 2638 2936 1267
Size: 879 492 298 2422
Fragments arranged by size:
2422 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 244 3795 244
Size: 3551 540
BsaAI yAC'GTr
Cuts at: 1444 1444
Size: 4091
BsaBI GATnn'nnATC
Cuts at: 6 1605 3642 6
Size: 1599 2037 455
Fragments arranged by size:
2037 1599 455
BsaHI Gr'CG_yC
Cuts at: 1522 1770 2472 3968 1522
Size: 248 702 1496 1645
Fragments arranged by size:
1645 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1280 1801 2479 2753 2900 3311 1280
Size: 521 678 274 147 411 2060
Fragments arranged by size:
2060 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 464 589 709 941 464
Size: 125 120 232 3614
Fragments arranged by size:
3614 232 125 120
BseYI C'CCAG_C
Cuts at: 606 888 1006 1516 2274 2851 3347 606
Size: 282 118 510 758 577 496 1350
Fragments arranged by size:
1350 758 577 510 496 282 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 964 964
Size: 4091
BsiEI CG_ry'CG
Cuts at: 104 1633 1679 2887 104
Size: 1529 46 1208 1308
Fragments arranged by size:
1529 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1105 1883 2073 2865 1105
Size: 778 190 792 2331
Fragments arranged by size:
2331 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1105 1716 1809 1883 2073 2135 2865 1105
Size: 611 93 74 190 62 730 2331
Fragments arranged by size:
2331 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3311 3311
Size: 4091
BspHI T'CATG_A
Cuts at: 3267 3267
Size: 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1141 1355 1657 2038 1141
Size: 214 302 381 3194
Fragments arranged by size:
3194 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2383 2437 3921 4086 50
Size: 2333 54 1484 165 55
Fragments arranged by size:
2333 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 620 2003 620
Size: 1383 2708
BsrFI r'CCGG_y
Cuts at: 2089 2270 2479 2089
Size: 181 209 3701
Fragments arranged by size:
3701 209 181
BssSI C'ACGA_G
Cuts at: 2362 2720 2362
Size: 358 3733
BstAPI GCAn_nnn'nTGC
Cuts at: 1468 1468
Size: 4091
BstBI TT'CG_AA
Cuts at: 2452 2452
Size: 4091
BstEII G'GTnAC_C
Cuts at: 1012 1012
Size: 4091
BstXI CCAn_nnnn'nTGG
Cuts at: 829 829
Size: 4091
BstYI r'GATC_y
Cuts at: 1 1116 1583 1606 1941 2187 3199 3285 3297
Size: 1115 467 23 335 246 1012 86 12
Cuts at: 3297 3643 1
Size: 346 449
Fragments arranged by size:
1115 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 345 345
Size: 4091
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2041 2222 2385 2041
Size: 181 163 3747
Fragments arranged by size:
3747 181 163
BtsI GCAGTG_nn'
Cuts at: 1334 1334
Size: 4091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 755 790 755
Size: 35 4056
DraI TTT'AAA
Cuts at: 707 1090 707
Size: 383 3708
DrdI GACnn_nn'nnGTC
Cuts at: 1797 2655 1797
Size: 858 3233
EaeI y'GGCC_r
Cuts at: 1676 1850 2241 2268 3613 1676
Size: 174 391 27 1345 2154
Fragments arranged by size:
2154 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1676 1676
Size: 4091
EarI CTCTTCn'nnn_
Cuts at: 376 918 2114 2324 376
Size: 542 1196 210 2143
Fragments arranged by size:
2143 1196 542 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2299 2609 2755 3901 2299
Size: 310 146 1146 2489
Fragments arranged by size:
2489 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1103 1103
Size: 4091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 771 866 923 1916 2348 3095 3363 268
Size: 503 95 57 993 432 747 268 996
Fragments arranged by size:
996 993 747 503 432 268 95 57
EcoNI CCTnn'n_nnAGG
Cuts at: 753 753
Size: 4091
EcoO109I rG'GnC_Cy
Cuts at: 1230 1230
Size: 4091
EcoRI G'AATT_C
Cuts at: 514 1095 514
Size: 581 3510
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 4091
FspI TGC'GCA
Cuts at: 1872 1872
Size: 4091
HaeII r_GCGC'y
Cuts at: 1525 1773 2795 3774 1525
Size: 248 1022 979 1842
Fragments arranged by size:
1842 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1933 1963 1965 1995 1933
Size: 30 2 30 4029
Fragments arranged by size:
4029 30 30 2
HincII GTy'rAC
Cuts at: 1130 1130
Size: 4091
HindIII A'AGCT_T
Cuts at: 501 501
Size: 4091
KasI G'GCGC_C
Cuts at: 1521 1769 1521
Size: 248 3843
KpnI G_GTAC'C
Cuts at: 1111 1111
Size: 4091
MluI A'CGCG_T
Cuts at: 3524 3524
Size: 4091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2762 2946 2762
Size: 184 3907
MscI TGG'CCA
Cuts at: 1852 1852
Size: 4091
MslI CAynn'nnrTG
Cuts at: 2080 2207 2080
Size: 127 3964
MspA1I CmG'CkG
Cuts at: 1010 1202 1516 1876 2889 3134 1010
Size: 192 314 360 1013 245 1967
Fragments arranged by size:
1967 1013 360 314 245 192
NaeI GCC'GGC
Cuts at: 2272 2272
Size: 4091
NarI GG'CG_CC
Cuts at: 1522 1770 1522
Size: 248 3843
NgoMIV G'CCGG_C
Cuts at: 2270 2270
Size: 4091
NsiI A_TGCA'T
Cuts at: 113 113
Size: 4091
NspI r_CATG'y
Cuts at: 1144 1644 2551 1144
Size: 500 907 2684
Fragments arranged by size:
2684 907 500
PciI A'CATG_T
Cuts at: 2547 2547
Size: 4091
PmeI GTTT'AAAC
Cuts at: 1090 1090
Size: 4091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3230 3262 3230
Size: 32 4059
PsiI TTA'TAA
Cuts at: 481 481
Size: 4091
PstI C_TGCA'G
Cuts at: 1138 1823 1138
Size: 685 3406
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4091
PvuII CAG'CTG
Cuts at: 1516 1876 1516
Size: 360 3731
RsrII CG'GwC_CG
Cuts at: 2286 2286
Size: 4091
SacI G_AGCT'C
Cuts at: 1105 1105
Size: 4091
SalI G'TCGA_C
Cuts at: 1128 1128
Size: 4091
SapI GCTCTTCn'nnn_
Cuts at: 2114 2324 2114
Size: 210 3881
SbfI CC_TGCA'GG
Cuts at: 1138 1138
Size: 4091
ScaI AGT'ACT
Cuts at: 936 981 3608 936
Size: 45 2627 1419
Fragments arranged by size:
2627 1419 45
SfcI C'TryA_G
Cuts at: 33 1134 1819 2812 3003 33
Size: 1101 685 993 191 1121
Fragments arranged by size:
1121 1101 993 685 191
SfoI GGC'GCC
Cuts at: 1523 1771 1523
Size: 248 3843
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
SmaI CCC'GGG
Cuts at: 1113 1113
Size: 4091
SmlI C'TyrA_G
Cuts at: 630 1246 2161 2653 2915 630
Size: 616 915 492 262 1806
Fragments arranged by size:
1806 915 616 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4091
SphI G_CATG'C
Cuts at: 1144 1644 1144
Size: 500 3591
StyI C'CwwG_G
Cuts at: 1225 1579 1225
Size: 354 3737
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1674 1807 2266 2475 1674
Size: 133 459 209 3290
Fragments arranged by size:
3290 459 209 133
TatI w'GTAC_w
Cuts at: 934 979 3606 934
Size: 45 2627 1419
Fragments arranged by size:
2627 1419 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3608 3640 3608
Size: 32 4059
Tth111I GACn'n_nGTC
Cuts at: 1888 1888
Size: 4091
XbaI T'CTAG_A
Cuts at: 1122 1122
Size: 4091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1586 1586
Size: 4091
XmaI C'CCGG_G
Cuts at: 1111 1111
Size: 4091
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PciI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII Bsu36I
BtgI ClaI DraIII EcoRV FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI