Restriction map for
pF1KB8273
(Circular) (Six-base) MAPSORT of: pF1KB8273.seq Check: 2214 from: 1 to: 3944
pF1KB8273 3944 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 982 982
Size: 3944
Acc65I G'GTAC_C
Cuts at: 960 960
Size: 3944
AclI AA'CG_TT
Cuts at: 3784 3784
Size: 3944
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 505 1769 2201 2948 3216 320
Size: 185 1264 432 747 268 1048
Fragments arranged by size:
1264 1048 747 432 268 185
AflIII A'CryG_T
Cuts at: 1296 2400 3377 1296
Size: 1104 977 1863
Fragments arranged by size:
1863 1104 977
AgeI A'CCGG_T
Cuts at: 2332 2332
Size: 3944
AlwNI CAG_nnn'CTG
Cuts at: 1249 2816 1249
Size: 1567 2377
ApaLI G'TGCA_C
Cuts at: 2714 2714
Size: 3944
ApoI r'AATT_y
Cuts at: 948 3297 3553 948
Size: 2349 256 1339
Fragments arranged by size:
2349 1339 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3944
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3944
AvaI C'yCGr_G
Cuts at: 964 964
Size: 3944
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 831 864 831
Size: 33 3911
BamHI G'GATC_C
Cuts at: 969 969
Size: 3944
BanI G'GyrC_C
Cuts at: 960 1374 1622 1657 960
Size: 414 248 35 3247
Fragments arranged by size:
3247 414 248 35
BanII G_rGCy'C
Cuts at: 431 742 958 1988 431
Size: 311 216 1030 2387
Fragments arranged by size:
2387 1030 311 216
BbeI G_GCGC'C
Cuts at: 1378 1626 1378
Size: 248 3696
BbsI GAAGACnn'nnnn_
Cuts at: 445 445
Size: 3944
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 225 259 225
Size: 34 3910
BciVI GTATCCnnnnn_n'
Cuts at: 199 1842 2603 3944 199
Size: 1643 761 1341 199
Fragments arranged by size:
1643 1341 761 199
BclI T'GATC_A
Cuts at: 1464 1464
Size: 3944
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3944
BglII A'GATC_T
Cuts at: 1459 3496 1459
Size: 2037 1907
BlpI GC'TnA_GC
Cuts at: 1056 1056
Size: 3944
Bme1580I G_kGCm'C
Cuts at: 812 1569 1662 2718 812
Size: 757 93 1056 2038
Fragments arranged by size:
2038 1056 757 93
BmrI ACTGGGnnnn_n'
Cuts at: 1156 1237 1572 3716 1156
Size: 81 335 2144 1384
Fragments arranged by size:
2144 1384 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 450 2311 450
Size: 1861 2083
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 913 445
Size: 468 3476
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1120 1999 2491 2789 1120
Size: 879 492 298 2275
Fragments arranged by size:
2275 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3648 3648
Size: 3944
BsaAI yAC'GTr
Cuts at: 1297 1297
Size: 3944
BsaBI GATnn'nnATC
Cuts at: 6 1458 3495 6
Size: 1452 2037 455
Fragments arranged by size:
2037 1452 455
BsaHI Gr'CG_yC
Cuts at: 1375 1623 2325 3821 1375
Size: 248 702 1496 1498
Fragments arranged by size:
1498 1496 702 248
BsaWI w'CCGG_w
Cuts at: 224 742 1133 1654 2332 2606 2753 3164 224
Size: 518 391 521 678 274 147 411 1004
Fragments arranged by size:
1004 678 521 518 411 391 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 424 454 533 563 724 754 424
Size: 30 79 30 161 30 3614
Fragments arranged by size:
3614 161 79 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 691 694 878 691
Size: 3 184 3757
Fragments arranged by size:
3757 184 3
BseYI C'CCAG_C
Cuts at: 247 373 404 1369 2127 2704 3200 247
Size: 126 31 965 758 577 496 991
Fragments arranged by size:
991 965 758 577 496 126 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 542
Size: 3944
BsiEI CG_ry'CG
Cuts at: 104 1486 1532 2740 104
Size: 1382 46 1208 1308
Fragments arranged by size:
1382 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 431 958 1736 1926 2718 431
Size: 527 778 190 792 1657
Fragments arranged by size:
1657 792 778 527 190
BsmI GAATG_Cn'
Cuts at: 177 177
Size: 3944
Bsp1286I G_dGCh'C
Cuts at: 431 742 812 958 1569 1662 1736 1926 1988
Size: 311 70 146 611 93 74 190 62
Cuts at: 1988 2718 431
Size: 730 1657
Fragments arranged by size:
1657 730 611 311 190 146 93 74
70 62
BspEI T'CCGG_A
Cuts at: 224 742 3164 224
Size: 518 2422 1004
Fragments arranged by size:
2422 1004 518
BspHI T'CATG_A
Cuts at: 3120 3120
Size: 3944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 994 1208 1510 1891 994
Size: 214 302 381 3047
Fragments arranged by size:
3047 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2236 2290 3774 3939 50
Size: 2186 54 1484 165 55
Fragments arranged by size:
2186 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 644 1856 644
Size: 1212 2732
BsrFI r'CCGG_y
Cuts at: 836 1942 2123 2332 836
Size: 1106 181 209 2448
Fragments arranged by size:
2448 1106 209 181
BssSI C'ACGA_G
Cuts at: 2215 2573 2215
Size: 358 3586
BstAPI GCAn_nnn'nTGC
Cuts at: 1321 1321
Size: 3944
BstBI TT'CG_AA
Cuts at: 2305 2305
Size: 3944
BstXI CCAn_nnnn'nTGG
Cuts at: 696 696
Size: 3944
BstYI r'GATC_y
Cuts at: 1 969 1436 1459 1794 2040 3052 3138 3150
Size: 968 467 23 335 246 1012 86 12
Cuts at: 3150 3496 1
Size: 346 449
Fragments arranged by size:
1012 968 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 614 107
Size: 507 3437
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 228 696 1894 2075 2238 228
Size: 468 1198 181 163 1934
Fragments arranged by size:
1934 1198 468 181 163
BtsI GCAGTG_nn'
Cuts at: 1187 1187
Size: 3944
DraI TTT'AAA
Cuts at: 943 943
Size: 3944
DrdI GACnn_nn'nnGTC
Cuts at: 763 1650 2508 763
Size: 887 858 2199
Fragments arranged by size:
2199 887 858
EaeI y'GGCC_r
Cuts at: 447 834 1529 1703 2094 2121 3466 447
Size: 387 695 174 391 27 1345 925
Fragments arranged by size:
1345 925 695 391 387 174 27
EagI C'GGCC_G
Cuts at: 1529 1529
Size: 3944
EarI CTCTTCn'nnn_
Cuts at: 1967 2177 1967
Size: 210 3734
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 784 2152 2462 2608 3754 784
Size: 1368 310 146 1146 974
Fragments arranged by size:
1368 1146 974 310 146
EcoICRI GAG'CTC
Cuts at: 429 956 429
Size: 527 3417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 445 505 913 1769 2201 2948 3216 320
Size: 125 60 408 856 432 747 268 1048
Fragments arranged by size:
1048 856 747 432 408 268 125 60
EcoO109I rG'GnC_Cy
Cuts at: 1083 1083
Size: 3944
EcoRI G'AATT_C
Cuts at: 948 948
Size: 3944
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1963 1963
Size: 3944
FspI TGC'GCA
Cuts at: 1725 1725
Size: 3944
HaeII r_GCGC'y
Cuts at: 1378 1626 2648 3627 1378
Size: 248 1022 979 1695
Fragments arranged by size:
1695 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 459 491 548 580 724 756 1786 1816 1818
Size: 32 57 32 144 32 1030 30 2
Cuts at: 1818 1848 459
Size: 30 2555
Fragments arranged by size:
2555 1030 144 57 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 983 983
Size: 3944
HindIII A'AGCT_T
Cuts at: 211 211
Size: 3944
KasI G'GCGC_C
Cuts at: 1374 1622 1374
Size: 248 3696
KpnI G_GTAC'C
Cuts at: 964 964
Size: 3944
MluI A'CGCG_T
Cuts at: 3377 3377
Size: 3944
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 120 443 2615 2799 120
Size: 323 2172 184 1265
Fragments arranged by size:
2172 1265 323 184
MscI TGG'CCA
Cuts at: 1705 1705
Size: 3944
MslI CAynn'nnrTG
Cuts at: 394 1933 2060 394
Size: 1539 127 2278
Fragments arranged by size:
2278 1539 127
MspA1I CmG'CkG
Cuts at: 382 718 1055 1369 1729 2742 2987 382
Size: 336 337 314 360 1013 245 1339
Fragments arranged by size:
1339 1013 360 337 336 314 245
NaeI GCC'GGC
Cuts at: 2125 2125
Size: 3944
NarI GG'CG_CC
Cuts at: 1375 1623 1375
Size: 248 3696
NcoI C'CATG_G
Cuts at: 107 614 107
Size: 507 3437
NgoMIV G'CCGG_C
Cuts at: 2123 2123
Size: 3944
NspI r_CATG'y
Cuts at: 997 1497 2404 997
Size: 500 907 2537
Fragments arranged by size:
2537 907 500
PciI A'CATG_T
Cuts at: 2400 2400
Size: 3944
PflMI CCAn_nnn'nTGG
Cuts at: 139 927 139
Size: 788 3156
PmeI GTTT'AAAC
Cuts at: 943 943
Size: 3944
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3083 3115 3083
Size: 32 3912
PstI C_TGCA'G
Cuts at: 387 867 991 1676 387
Size: 480 124 685 2655
Fragments arranged by size:
2655 685 480 124
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3944
PvuII CAG'CTG
Cuts at: 382 718 1369 1729 382
Size: 336 651 360 2597
Fragments arranged by size:
2597 651 360 336
RsrII CG'GwC_CG
Cuts at: 2139 2139
Size: 3944
SacI G_AGCT'C
Cuts at: 431 958 431
Size: 527 3417
SalI G'TCGA_C
Cuts at: 981 981
Size: 3944
SapI GCTCTTCn'nnn_
Cuts at: 1967 2177 1967
Size: 210 3734
SbfI CC_TGCA'GG
Cuts at: 991 991
Size: 3944
ScaI AGT'ACT
Cuts at: 3461 3461
Size: 3944
SfcI C'TryA_G
Cuts at: 33 383 567 863 987 1672 2665 2856 33
Size: 350 184 296 124 685 993 191 1121
Fragments arranged by size:
1121 993 685 350 296 191 184 124
SfoI GGC'GCC
Cuts at: 1376 1624 1376
Size: 248 3696
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3944
SmaI CCC'GGG
Cuts at: 966 966
Size: 3944
SmlI C'TyrA_G
Cuts at: 1099 2014 2506 2768 1099
Size: 915 492 262 2275
Fragments arranged by size:
2275 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3944
SphI G_CATG'C
Cuts at: 997 1497 997
Size: 500 3444
SspI AAT'ATT
Cuts at: 357 357
Size: 3944
StyI C'CwwG_G
Cuts at: 107 614 629 1078 1432 107
Size: 507 15 449 354 2619
Fragments arranged by size:
2619 507 449 354 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1527 1660 2119 2328 1527
Size: 133 459 209 3143
Fragments arranged by size:
3143 459 209 133
TatI w'GTAC_w
Cuts at: 3459 3459
Size: 3944
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 424 456 3461 3493 424
Size: 32 3005 32 875
Fragments arranged by size:
3005 875 32 32
Tth111I GACn'n_nGTC
Cuts at: 1741 1741
Size: 3944
XbaI T'CTAG_A
Cuts at: 975 975
Size: 3944
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 249 1439 249
Size: 1190 2754
XmaI C'CCGG_G
Cuts at: 964 964
Size: 3944
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI