Restriction map for
pF1KB8271
(Circular) (Six-base) MAPSORT of: pF1KB8271.seq Check: 5755 from: 1 to: 4064
pF1KB8271 4064 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 288 590 855 288
Size: 302 265 3497
Fragments arranged by size:
3497 302 265
AccI GT'mk_AC
Cuts at: 1102 1102
Size: 4064
Acc65I G'GTAC_C
Cuts at: 1080 1080
Size: 4064
AclI AA'CG_TT
Cuts at: 3904 3904
Size: 4064
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1889 2321 3068 3336 1889
Size: 432 747 268 2617
Fragments arranged by size:
2617 747 432 268
AfeI AGC'GCT
Cuts at: 235 235
Size: 4064
AflIII A'CryG_T
Cuts at: 491 1416 2520 3497 491
Size: 925 1104 977 1058
Fragments arranged by size:
1104 1058 977 925
AgeI A'CCGG_T
Cuts at: 2452 2452
Size: 4064
AlwNI CAG_nnn'CTG
Cuts at: 288 339 1369 2936 288
Size: 51 1030 1567 1416
Fragments arranged by size:
1567 1416 1030 51
ApaI G_GGCC'C
Cuts at: 660 710 783 660
Size: 50 73 3941
Fragments arranged by size:
3941 73 50
ApaLI G'TGCA_C
Cuts at: 2834 2834
Size: 4064
ApoI r'AATT_y
Cuts at: 1068 3417 3673 1068
Size: 2349 256 1459
Fragments arranged by size:
2349 1459 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4064
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
AvaI C'yCGr_G
Cuts at: 196 1084 196
Size: 888 3176
BamHI G'GATC_C
Cuts at: 1089 1089
Size: 4064
BanI G'GyrC_C
Cuts at: 667 1024 1080 1494 1742 1777 667
Size: 357 56 414 248 35 2954
Fragments arranged by size:
2954 414 357 248 56 35
BanII G_rGCy'C
Cuts at: 197 660 710 751 783 962 1078 2108 197
Size: 463 50 41 32 179 116 1030 2153
Fragments arranged by size:
2153 1030 463 179 116 50 41 32
BbeI G_GCGC'C
Cuts at: 671 1498 1746 671
Size: 827 248 2989
Fragments arranged by size:
2989 827 248
BbsI GAAGACnn'nnnn_
Cuts at: 977 977
Size: 4064
BbvCI CC'TCA_GC
Cuts at: 682 682
Size: 4064
BciVI GTATCCnnnnn_n'
Cuts at: 1962 2723 4064 1962
Size: 761 1341 1962
Fragments arranged by size:
1962 1341 761
BclI T'GATC_A
Cuts at: 1584 1584
Size: 4064
BglI GCCn_nnn'nGGC
Cuts at: 934 934
Size: 4064
BglII A'GATC_T
Cuts at: 1579 3616 1579
Size: 2037 2027
BlpI GC'TnA_GC
Cuts at: 316 1176 316
Size: 860 3204
Bme1580I G_kGCm'C
Cuts at: 435 660 710 783 1027 1046 1689 1782 2838
Size: 225 50 73 244 19 643 93 1056
Cuts at: 2838 435
Size: 1661
Fragments arranged by size:
1661 1056 643 244 225 93 73 50
19
BmgBI CAC'GTC
Cuts at: 492 492
Size: 4064
BmrI ACTGGGnnnn_n'
Cuts at: 259 1276 1357 1692 3836 259
Size: 1017 81 335 2144 487
Fragments arranged by size:
2144 1017 487 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2431 2431
Size: 4064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 910 415
Size: 495 3569
Bpu10I CC'TnA_GC
Cuts at: 682 682
Size: 4064
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 1240 2119 2611 2909 928
Size: 312 879 492 298 2083
Fragments arranged by size:
2083 879 492 312 298
BsaI GGTCTCn'nnnn_
Cuts at: 352 3768 352
Size: 3416 648
BsaAI yAC'GTr
Cuts at: 1417 1417
Size: 4064
BsaBI GATnn'nnATC
Cuts at: 6 1578 3615 6
Size: 1572 2037 455
Fragments arranged by size:
2037 1572 455
BsaHI Gr'CG_yC
Cuts at: 668 1495 1743 2445 3941 668
Size: 827 248 702 1496 791
Fragments arranged by size:
1496 827 791 702 248
BsaWI w'CCGG_w
Cuts at: 1253 1774 2452 2726 2873 3284 1253
Size: 521 678 274 147 411 2033
Fragments arranged by size:
2033 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 478 508 478
Size: 30 4034
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 322 436 568 595 832 907 322
Size: 114 132 27 237 75 3479
Fragments arranged by size:
3479 237 132 114 75 27
BseYI C'CCAG_C
Cuts at: 236 275 471 902 1045 1489 2247 2824 3320
Size: 39 196 431 143 444 758 577 496
Cuts at: 3320 236
Size: 980
Fragments arranged by size:
980 758 577 496 444 431 196 143
39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 322 529 547 1030 301
Size: 21 207 18 483 3335
Fragments arranged by size:
3335 483 207 21 18
BsiEI CG_ry'CG
Cuts at: 104 501 843 1606 1652 2860 104
Size: 397 342 763 46 1208 1308
Fragments arranged by size:
1308 1208 763 397 342 46
BsiHKAI G_wGCw'C
Cuts at: 550 1078 1856 2046 2838 550
Size: 528 778 190 792 1776
Fragments arranged by size:
1776 792 778 528 190
BsmBI CGTCTCn'nnnn_
Cuts at: 418 418
Size: 4064
Bsp1286I G_dGCh'C
Cuts at: 197 435 550 660 710 751 783 962 1027
Size: 238 115 110 50 41 32 179 65
Cuts at: 1027 1046 1078 1689 1782 1856 2046 2108 2838
Size: 19 32 611 93 74 190 62 730
Cuts at: 2838 197
Size: 1423
Fragments arranged by size:
1423 730 611 238 190 179 115 110
93 74 65 62 50 41 32 32
19
BspEI T'CCGG_A
Cuts at: 3284 3284
Size: 4064
BspHI T'CATG_A
Cuts at: 3240 3240
Size: 4064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 278 288 590 632 855 1114 1328 1630 2011
Size: 10 302 42 223 259 214 302 381
Cuts at: 2011 278
Size: 2331
Fragments arranged by size:
2331 381 302 302 259 223 214 42
10
BsrBI CCG'CTC
Cuts at: 50 227 777 798 2356 2410 3894 4059 50
Size: 177 550 21 1558 54 1484 165 55
Fragments arranged by size:
1558 1484 550 177 165 55 54 21
BsrDI GCAATG_nn'
Cuts at: 1976 1976
Size: 4064
BsrFI r'CCGG_y
Cuts at: 757 2062 2243 2452 757
Size: 1305 181 209 2369
Fragments arranged by size:
2369 1305 209 181
BssHII G'CGCG_C
Cuts at: 804 806 879 804
Size: 2 73 3989
Fragments arranged by size:
3989 73 2
BssSI C'ACGA_G
Cuts at: 2335 2693 2335
Size: 358 3706
BstAPI GCAn_nnn'nTGC
Cuts at: 534 1441 534
Size: 907 3157
BstBI TT'CG_AA
Cuts at: 2425 2425
Size: 4064
BstEII G'GTnAC_C
Cuts at: 327 327
Size: 4064
BstYI r'GATC_y
Cuts at: 1 1089 1556 1579 1914 2160 3172 3258 3270
Size: 1088 467 23 335 246 1012 86 12
Cuts at: 3270 3616 1
Size: 346 449
Fragments arranged by size:
1088 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 516 1019 516
Size: 503 3561
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 626 2014 2195 2358 626
Size: 1388 181 163 2332
Fragments arranged by size:
2332 1388 181 163
BtsI GCAGTG_nn'
Cuts at: 647 1307 647
Size: 660 3404
DraI TTT'AAA
Cuts at: 1063 1063
Size: 4064
DrdI GACnn_nn'nnGTC
Cuts at: 1770 2628 1770
Size: 858 3206
EaeI y'GGCC_r
Cuts at: 228 498 540 755 759 1649 1823 2214 2241
Size: 270 42 215 4 890 174 391 27
Cuts at: 2241 3586 228
Size: 1345 706
Fragments arranged by size:
1345 890 706 391 270 215 174 42
27 4
EagI C'GGCC_G
Cuts at: 498 1649 498
Size: 1151 2913
EarI CTCTTCn'nnn_
Cuts at: 2087 2297 2087
Size: 210 3854
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 433 886 2272 2582 2728 3874 433
Size: 453 1386 310 146 1146 623
Fragments arranged by size:
1386 1146 623 453 310 146
EcoICRI GAG'CTC
Cuts at: 1076 1076
Size: 4064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 910 1889 2321 3068 3336 415
Size: 495 979 432 747 268 1143
Fragments arranged by size:
1143 979 747 495 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 850 850
Size: 4064
EcoO109I rG'GnC_Cy
Cuts at: 656 706 707 1203 656
Size: 50 1 496 3517
Fragments arranged by size:
3517 496 50 1
EcoRI G'AATT_C
Cuts at: 1068 1068
Size: 4064
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 4064
FseI GG_CCGG'CC
Cuts at: 761 761
Size: 4064
FspI TGC'GCA
Cuts at: 586 1845 586
Size: 1259 2805
HaeII r_GCGC'y
Cuts at: 237 349 636 671 689 702 984 1034 1498
Size: 112 287 35 18 13 282 50 464
Cuts at: 1498 1746 2768 3747 237
Size: 248 1022 979 554
Fragments arranged by size:
1022 979 554 464 287 282 248 112
50 35 18 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1906 1936 1938 1968 1906
Size: 30 2 30 4002
Fragments arranged by size:
4002 30 30 2
HincII GTy'rAC
Cuts at: 1103 1103
Size: 4064
KasI G'GCGC_C
Cuts at: 667 1494 1742 667
Size: 827 248 2989
Fragments arranged by size:
2989 827 248
KpnI G_GTAC'C
Cuts at: 1084 1084
Size: 4064
MluI A'CGCG_T
Cuts at: 3497 3497
Size: 4064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2735 2919 2735
Size: 184 3880
MscI TGG'CCA
Cuts at: 1825 1825
Size: 4064
MslI CAynn'nnrTG
Cuts at: 2053 2180 2053
Size: 127 3937
MspA1I CmG'CkG
Cuts at: 518 1175 1489 1849 2862 3107 518
Size: 657 314 360 1013 245 1475
Fragments arranged by size:
1475 1013 657 360 314 245
NaeI GCC'GGC
Cuts at: 759 2245 759
Size: 1486 2578
NarI GG'CG_CC
Cuts at: 668 1495 1743 668
Size: 827 248 2989
Fragments arranged by size:
2989 827 248
NgoMIV G'CCGG_C
Cuts at: 757 2243 757
Size: 1486 2578
NotI GC'GGCC_GC
Cuts at: 498 498
Size: 4064
NspI r_CATG'y
Cuts at: 978 1117 1617 2524 978
Size: 139 500 907 2518
Fragments arranged by size:
2518 907 500 139
PciI A'CATG_T
Cuts at: 2520 2520
Size: 4064
PmeI GTTT'AAAC
Cuts at: 1063 1063
Size: 4064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3203 3235 3203
Size: 32 4032
PspOMI G'GGCC_C
Cuts at: 656 706 779 656
Size: 50 73 3941
Fragments arranged by size:
3941 73 50
PstI C_TGCA'G
Cuts at: 455 629 920 1111 1796 455
Size: 174 291 191 685 2723
Fragments arranged by size:
2723 685 291 191 174
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4064
PvuII CAG'CTG
Cuts at: 1489 1849 1489
Size: 360 3704
RsrII CG'GwC_CG
Cuts at: 2259 2259
Size: 4064
SacI G_AGCT'C
Cuts at: 1078 1078
Size: 4064
SacII CC_GC'GG
Cuts at: 519 519
Size: 4064
SalI G'TCGA_C
Cuts at: 1101 1101
Size: 4064
SapI GCTCTTCn'nnn_
Cuts at: 2087 2297 2087
Size: 210 3854
SbfI CC_TGCA'GG
Cuts at: 920 1111 920
Size: 191 3873
ScaI AGT'ACT
Cuts at: 3581 3581
Size: 4064
SfcI C'TryA_G
Cuts at: 33 451 625 769 916 1107 1792 2785 2976
Size: 418 174 144 147 191 685 993 191
Cuts at: 2976 33
Size: 1121
Fragments arranged by size:
1121 993 685 418 191 191 174 147
144
SfiI GGCCn_nnn'nGGCC
Cuts at: 934 934
Size: 4064
SfoI GGC'GCC
Cuts at: 669 1496 1744 669
Size: 827 248 2989
Fragments arranged by size:
2989 827 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
SmaI CCC'GGG
Cuts at: 1086 1086
Size: 4064
SmlI C'TyrA_G
Cuts at: 943 1219 2134 2626 2888 943
Size: 276 915 492 262 2119
Fragments arranged by size:
2119 915 492 276 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4064
SphI G_CATG'C
Cuts at: 1117 1617 1117
Size: 500 3564
StuI AGG'CCT
Cuts at: 379 787 928 379
Size: 408 141 3515
Fragments arranged by size:
3515 408 141
StyI C'CwwG_G
Cuts at: 161 443 1198 1552 161
Size: 282 755 354 2673
Fragments arranged by size:
2673 755 354 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 229 267 346 1647 1780 2239 2448 229
Size: 38 79 1301 133 459 209 1845
Fragments arranged by size:
1845 1301 459 209 133 79 38
TatI w'GTAC_w
Cuts at: 3579 3579
Size: 4064
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 478 510 3581 3613 478
Size: 32 3071 32 929
Fragments arranged by size:
3071 929 32 32
Tth111I GACn'n_nGTC
Cuts at: 288 1861 288
Size: 1573 2491
XbaI T'CTAG_A
Cuts at: 1095 1095
Size: 4064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1559 1559
Size: 4064
XmaI C'CCGG_G
Cuts at: 1084 1084
Size: 4064
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NotI NspI PciI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BmtI BsiWI BsmI BsrGI BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FspAI HindIII
HpaI MfeI NcoI NdeI NheI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SanDI SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI