Restriction map for
pF1KB8270
(Circular) (Six-base) MAPSORT of: pF1KB8270.seq Check: 6365 from: 1 to: 3920
pF1KB8270 3920 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 235 235
Size: 3920
AatII G_ACGT'C
Cuts at: 821 821
Size: 3920
AccI GT'mk_AC
Cuts at: 958 958
Size: 3920
Acc65I G'GTAC_C
Cuts at: 936 936
Size: 3920
AclI AA'CG_TT
Cuts at: 3760 3760
Size: 3920
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 313 874 1745 2177 2924 3192 295
Size: 18 561 871 432 747 268 1023
Fragments arranged by size:
1023 871 747 561 432 268 18
AflIII A'CryG_T
Cuts at: 1272 2376 3353 1272
Size: 1104 977 1839
Fragments arranged by size:
1839 1104 977
AgeI A'CCGG_T
Cuts at: 656 2308 656
Size: 1652 2268
AlwNI CAG_nnn'CTG
Cuts at: 1225 2792 1225
Size: 1567 2353
ApaLI G'TGCA_C
Cuts at: 2690 2690
Size: 3920
ApoI r'AATT_y
Cuts at: 924 3273 3529 924
Size: 2349 256 1315
Fragments arranged by size:
2349 1315 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3920
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
AvaI C'yCGr_G
Cuts at: 379 811 940 379
Size: 432 129 3359
Fragments arranged by size:
3359 432 129
BamHI G'GATC_C
Cuts at: 945 945
Size: 3920
BanI G'GyrC_C
Cuts at: 151 263 667 936 1350 1598 1633 151
Size: 112 404 269 414 248 35 2438
Fragments arranged by size:
2438 414 404 269 248 112 35
BanII G_rGCy'C
Cuts at: 386 685 934 1964 386
Size: 299 249 1030 2342
Fragments arranged by size:
2342 1030 299 249
BbeI G_GCGC'C
Cuts at: 1354 1602 1354
Size: 248 3672
BbsI GAAGACnn'nnnn_
Cuts at: 177 206 363 177
Size: 29 157 3734
Fragments arranged by size:
3734 157 29
BbvCI CC'TCA_GC
Cuts at: 758 758
Size: 3920
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 564 598 564
Size: 34 3886
BciVI GTATCCnnnnn_n'
Cuts at: 1818 2579 3920 1818
Size: 761 1341 1818
Fragments arranged by size:
1818 1341 761
BclI T'GATC_A
Cuts at: 1440 1440
Size: 3920
BglI GCCn_nnn'nGGC
Cuts at: 112 169 112
Size: 57 3863
BglII A'GATC_T
Cuts at: 1435 3472 1435
Size: 2037 1883
BlpI GC'TnA_GC
Cuts at: 1032 1032
Size: 3920
Bme1580I G_kGCm'C
Cuts at: 154 266 394 1545 1638 2694 154
Size: 112 128 1151 93 1056 1380
Fragments arranged by size:
1380 1151 1056 128 112 93
BmrI ACTGGGnnnn_n'
Cuts at: 525 1132 1213 1548 3692 525
Size: 607 81 335 2144 753
Fragments arranged by size:
2144 753 607 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 3920
Bpu10I CC'TnA_GC
Cuts at: 758 758
Size: 3920
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1096 1975 2467 2765 1096
Size: 879 492 298 2251
Fragments arranged by size:
2251 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3624 3624
Size: 3920
BsaAI yAC'GTr
Cuts at: 1273 1273
Size: 3920
BsaBI GATnn'nnATC
Cuts at: 6 1434 3471 6
Size: 1428 2037 455
Fragments arranged by size:
2037 1428 455
BsaHI Gr'CG_yC
Cuts at: 818 836 1351 1599 2301 3797 818
Size: 18 515 248 702 1496 941
Fragments arranged by size:
1496 941 702 515 248 18
BsaWI w'CCGG_w
Cuts at: 656 1109 1630 2308 2582 2729 3140 656
Size: 453 521 678 274 147 411 1436
Fragments arranged by size:
1436 678 521 453 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 148 178 193 223 148
Size: 30 15 30 3845
Fragments arranged by size:
3845 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 211
Size: 3920
BseYI C'CCAG_C
Cuts at: 132 147 1345 2103 2680 3176 132
Size: 15 1198 758 577 496 876
Fragments arranged by size:
1198 876 758 577 496 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 830 830
Size: 3920
BsiEI CG_ry'CG
Cuts at: 104 1462 1508 2716 104
Size: 1358 46 1208 1308
Fragments arranged by size:
1358 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 934 1712 1902 2694 934
Size: 778 190 792 2160
Fragments arranged by size:
2160 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 460 460
Size: 3920
Bsp1286I G_dGCh'C
Cuts at: 154 266 386 394 685 934 1545 1638 1712
Size: 112 120 8 291 249 611 93 74
Cuts at: 1712 1902 1964 2694 154
Size: 190 62 730 1380
Fragments arranged by size:
1380 730 611 291 249 190 120 112
93 74 62 8
BspEI T'CCGG_A
Cuts at: 3140 3140
Size: 3920
BspHI T'CATG_A
Cuts at: 557 3096 557
Size: 2539 1381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 235 566 970 1184 1486 1867 235
Size: 331 404 214 302 381 2288
Fragments arranged by size:
2288 404 381 331 302 214
BsrBI CCG'CTC
Cuts at: 50 2212 2266 3750 3915 50
Size: 2162 54 1484 165 55
Fragments arranged by size:
2162 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1832 1832
Size: 3920
BsrFI r'CCGG_y
Cuts at: 175 602 656 838 1918 2099 2308 175
Size: 427 54 182 1080 181 209 1787
Fragments arranged by size:
1787 1080 427 209 182 181 54
BssSI C'ACGA_G
Cuts at: 2191 2549 2191
Size: 358 3562
BstAPI GCAn_nnn'nTGC
Cuts at: 1297 1297
Size: 3920
BstBI TT'CG_AA
Cuts at: 2281 2281
Size: 3920
BstEII G'GTnAC_C
Cuts at: 774 774
Size: 3920
BstYI r'GATC_y
Cuts at: 1 945 1412 1435 1770 2016 3028 3114 3126
Size: 944 467 23 335 246 1012 86 12
Cuts at: 3126 3472 1
Size: 346 449
Fragments arranged by size:
1012 944 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 156 386 411 107
Size: 49 230 25 3616
Fragments arranged by size:
3616 230 49 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1870 2051 2214 1870
Size: 181 163 3576
Fragments arranged by size:
3576 181 163
BtsI GCAGTG_nn'
Cuts at: 1163 1163
Size: 3920
DraI TTT'AAA
Cuts at: 919 919
Size: 3920
DrdI GACnn_nn'nnGTC
Cuts at: 1626 2484 1626
Size: 858 3062
EaeI y'GGCC_r
Cuts at: 312 395 470 648 725 1505 1679 2070 2097
Size: 83 75 178 77 780 174 391 27
Cuts at: 2097 3442 312
Size: 1345 790
Fragments arranged by size:
1345 790 780 391 178 174 83 77
75 27
EagI C'GGCC_G
Cuts at: 1505 1505
Size: 3920
EarI CTCTTCn'nnn_
Cuts at: 850 915 1943 2153 850
Size: 65 1028 210 2617
Fragments arranged by size:
2617 1028 210 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 886 2128 2438 2584 3730 886
Size: 1242 310 146 1146 1076
Fragments arranged by size:
1242 1146 1076 310 146
EcoICRI GAG'CTC
Cuts at: 932 932
Size: 3920
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 313 874 1745 2177 2924 3192 295
Size: 18 561 871 432 747 268 1023
Fragments arranged by size:
1023 871 747 561 432 268 18
EcoO109I rG'GnC_Cy
Cuts at: 552 1059 552
Size: 507 3413
EcoRI G'AATT_C
Cuts at: 924 924
Size: 3920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1939 1939
Size: 3920
FspI TGC'GCA
Cuts at: 715 1701 715
Size: 986 2934
HaeII r_GCGC'y
Cuts at: 248 804 1354 1602 2624 3603 248
Size: 556 550 248 1022 979 565
Fragments arranged by size:
1022 979 565 556 550 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 193 225 826 858 1762 1792 1794 1824 193
Size: 32 601 32 904 30 2 30 2289
Fragments arranged by size:
2289 904 601 32 32 30 30 2
HincII GTy'rAC
Cuts at: 959 959
Size: 3920
KasI G'GCGC_C
Cuts at: 1350 1598 1350
Size: 248 3672
KpnI G_GTAC'C
Cuts at: 940 940
Size: 3920
MluI A'CGCG_T
Cuts at: 3353 3353
Size: 3920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 710 2591 2775 710
Size: 1881 184 1855
Fragments arranged by size:
1881 1855 184
MscI TGG'CCA
Cuts at: 1681 1681
Size: 3920
MslI CAynn'nnrTG
Cuts at: 875 1909 2036 875
Size: 1034 127 2759
Fragments arranged by size:
2759 1034 127
MspA1I CmG'CkG
Cuts at: 269 863 1031 1345 1705 2718 2963 269
Size: 594 168 314 360 1013 245 1226
Fragments arranged by size:
1226 1013 594 360 314 245 168
NaeI GCC'GGC
Cuts at: 177 840 2101 177
Size: 663 1261 1996
Fragments arranged by size:
1996 1261 663
NarI GG'CG_CC
Cuts at: 1351 1599 1351
Size: 248 3672
NcoI C'CATG_G
Cuts at: 107 386 107
Size: 279 3641
NgoMIV G'CCGG_C
Cuts at: 175 838 2099 175
Size: 663 1261 1996
Fragments arranged by size:
1996 1261 663
NspI r_CATG'y
Cuts at: 973 1473 2380 973
Size: 500 907 2513
Fragments arranged by size:
2513 907 500
PciI A'CATG_T
Cuts at: 2376 2376
Size: 3920
PfoI T'CCnGG_A
Cuts at: 821 821
Size: 3920
PmeI GTTT'AAAC
Cuts at: 919 919
Size: 3920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 562 594 3059 3091 562
Size: 32 2465 32 1391
Fragments arranged by size:
2465 1391 32 32
PstI C_TGCA'G
Cuts at: 967 1652 967
Size: 685 3235
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3920
PvuII CAG'CTG
Cuts at: 1345 1705 1345
Size: 360 3560
RsrII CG'GwC_CG
Cuts at: 2115 2115
Size: 3920
SacI G_AGCT'C
Cuts at: 934 934
Size: 3920
SalI G'TCGA_C
Cuts at: 957 957
Size: 3920
SapI GCTCTTCn'nnn_
Cuts at: 850 1943 2153 850
Size: 1093 210 2617
Fragments arranged by size:
2617 1093 210
SbfI CC_TGCA'GG
Cuts at: 967 967
Size: 3920
ScaI AGT'ACT
Cuts at: 361 3437 361
Size: 3076 844
SfcI C'TryA_G
Cuts at: 33 963 1648 2641 2832 33
Size: 930 685 993 191 1121
Fragments arranged by size:
1121 993 930 685 191
SfoI GGC'GCC
Cuts at: 1352 1600 1352
Size: 248 3672
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
SmaI CCC'GGG
Cuts at: 942 942
Size: 3920
SmlI C'TyrA_G
Cuts at: 1075 1990 2482 2744 1075
Size: 915 492 262 2251
Fragments arranged by size:
2251 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3920
SphI G_CATG'C
Cuts at: 973 1473 973
Size: 500 3420
StyI C'CwwG_G
Cuts at: 107 204 386 677 1054 1408 107
Size: 97 182 291 377 354 2619
Fragments arranged by size:
2619 377 354 291 182 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 169 1503 1636 2095 2304 169
Size: 1334 133 459 209 1785
Fragments arranged by size:
1785 1334 459 209 133
TatI w'GTAC_w
Cuts at: 359 3435 359
Size: 3076 844
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 148 180 334 366 3437 3469 148
Size: 32 154 32 3071 32 599
Fragments arranged by size:
3071 599 154 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1717 1717
Size: 3920
XbaI T'CTAG_A
Cuts at: 951 951
Size: 3920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1415 1415
Size: 3920
XmaI C'CCGG_G
Cuts at: 940 940
Size: 3920
ZraI GAC'GTC
Cuts at: 819 819
Size: 3920
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BfrBI BmgBI BmtI BpmI BsiWI BsmI
BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI