Restriction map for
pF1KB8254
(Circular) (Six-base) MAPSORT of: pF1KB8254.seq Check: 6201 from: 1 to: 3689
pF1KB8254 3689 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 727 727
Size: 3689
Acc65I G'GTAC_C
Cuts at: 705 705
Size: 3689
AclI AA'CG_TT
Cuts at: 3529 3529
Size: 3689
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 1514 1946 2693 2961 514
Size: 1000 432 747 268 1242
Fragments arranged by size:
1242 1000 747 432 268
AflIII A'CryG_T
Cuts at: 1041 2145 3122 1041
Size: 1104 977 1608
Fragments arranged by size:
1608 1104 977
AgeI A'CCGG_T
Cuts at: 255 2077 255
Size: 1822 1867
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 654 654
Size: 3689
AlwNI CAG_nnn'CTG
Cuts at: 994 2561 994
Size: 1567 2122
ApaLI G'TGCA_C
Cuts at: 2459 2459
Size: 3689
ApoI r'AATT_y
Cuts at: 693 3042 3298 693
Size: 2349 256 1084
Fragments arranged by size:
2349 1084 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3689
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3689
AvaI C'yCGr_G
Cuts at: 709 709
Size: 3689
BamHI G'GATC_C
Cuts at: 714 714
Size: 3689
BanI G'GyrC_C
Cuts at: 413 705 1119 1367 1402 413
Size: 292 414 248 35 2700
Fragments arranged by size:
2700 414 292 248 35
BanII G_rGCy'C
Cuts at: 703 1733 703
Size: 1030 2659
BbeI G_GCGC'C
Cuts at: 1123 1371 1123
Size: 248 3441
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3655
BciVI GTATCCnnnnn_n'
Cuts at: 270 410 1587 2348 3689 270
Size: 140 1177 761 1341 270
Fragments arranged by size:
1341 1177 761 270 140
BclI T'GATC_A
Cuts at: 167 1209 167
Size: 1042 2647
BfrBI ATG'CAT
Cuts at: 187 187
Size: 3689
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3689
BglII A'GATC_T
Cuts at: 1204 3241 1204
Size: 2037 1652
BlpI GC'TnA_GC
Cuts at: 801 801
Size: 3689
Bme1580I G_kGCm'C
Cuts at: 1314 1407 2463 1314
Size: 93 1056 2540
Fragments arranged by size:
2540 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 901 982 1317 3461 901
Size: 81 335 2144 1129
Fragments arranged by size:
2144 1129 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 652 2056 652
Size: 1404 2285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 592 215
Size: 377 3312
Bpu10I CC'TnA_GC
Cuts at: 545 545
Size: 3689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 865 1744 2236 2534 586
Size: 279 879 492 298 1741
Fragments arranged by size:
1741 879 492 298 279
BsaI GGTCTCn'nnnn_
Cuts at: 3393 3393
Size: 3689
BsaAI yAC'GTr
Cuts at: 1042 1042
Size: 3689
BsaBI GATnn'nnATC
Cuts at: 6 1203 3240 6
Size: 1197 2037 455
Fragments arranged by size:
2037 1197 455
BsaHI Gr'CG_yC
Cuts at: 1120 1368 2070 3566 1120
Size: 248 702 1496 1243
Fragments arranged by size:
1496 1243 702 248
BsaWI w'CCGG_w
Cuts at: 255 878 1399 2077 2351 2498 2909 255
Size: 623 521 678 274 147 411 1035
Fragments arranged by size:
1035 678 623 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1114 1872 2449 2945 1114
Size: 758 577 496 1858
Fragments arranged by size:
1858 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 485 1231 1277 2485 104
Size: 381 746 46 1208 1308
Fragments arranged by size:
1308 1208 746 381 46
BsiHKAI G_wGCw'C
Cuts at: 580 703 1481 1671 2463 580
Size: 123 778 190 792 1806
Fragments arranged by size:
1806 792 778 190 123
BsmBI CGTCTCn'nnnn_
Cuts at: 131 131
Size: 3689
Bsp1286I G_dGCh'C
Cuts at: 580 703 1314 1407 1481 1671 1733 2463 580
Size: 123 611 93 74 190 62 730 1806
Fragments arranged by size:
1806 730 611 190 123 93 74 62
BspEI T'CCGG_A
Cuts at: 2909 2909
Size: 3689
BspHI T'CATG_A
Cuts at: 2865 2865
Size: 3689
BspMI ACCTGCnnnn'nnnn_
Cuts at: 739 953 1255 1636 739
Size: 214 302 381 2792
Fragments arranged by size:
2792 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1981 2035 3519 3684 50
Size: 1931 54 1484 165 55
Fragments arranged by size:
1931 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1601 1601
Size: 3689
BsrFI r'CCGG_y
Cuts at: 255 1687 1868 2077 255
Size: 1432 181 209 1867
Fragments arranged by size:
1867 1432 209 181
BssSI C'ACGA_G
Cuts at: 1960 2318 1960
Size: 358 3331
BstAPI GCAn_nnn'nTGC
Cuts at: 1066 1066
Size: 3689
BstBI TT'CG_AA
Cuts at: 2050 2050
Size: 3689
BstXI CCAn_nnnn'nTGG
Cuts at: 240 240
Size: 3689
BstYI r'GATC_y
Cuts at: 1 714 1181 1204 1539 1785 2797 2883 2895
Size: 713 467 23 335 246 1012 86 12
Cuts at: 2895 3241 1
Size: 346 449
Fragments arranged by size:
1012 713 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 404 404
Size: 3689
BtgI C'CryG_G
Cuts at: 539 539
Size: 3689
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1639 1820 1983 1639
Size: 181 163 3345
Fragments arranged by size:
3345 181 163
BtsI GCAGTG_nn'
Cuts at: 932 932
Size: 3689
DraI TTT'AAA
Cuts at: 688 688
Size: 3689
DrdI GACnn_nn'nnGTC
Cuts at: 1395 2253 1395
Size: 858 2831
EaeI y'GGCC_r
Cuts at: 1274 1448 1839 1866 3211 1274
Size: 174 391 27 1345 1752
Fragments arranged by size:
1752 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1274 1274
Size: 3689
EarI CTCTTCn'nnn_
Cuts at: 652 658 1712 1922 652
Size: 6 1054 210 2419
Fragments arranged by size:
2419 1054 210 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1897 2207 2353 3499 1897
Size: 310 146 1146 2087
Fragments arranged by size:
2087 1146 310 146
EcoICRI GAG'CTC
Cuts at: 701 701
Size: 3689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 514 592 1514 1946 2693 2961 215
Size: 299 78 922 432 747 268 943
Fragments arranged by size:
943 922 747 432 299 268 78
EcoO109I rG'GnC_Cy
Cuts at: 828 828
Size: 3689
EcoRI G'AATT_C
Cuts at: 693 693
Size: 3689
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1708 1708
Size: 3689
FspI TGC'GCA
Cuts at: 1470 1470
Size: 3689
HaeII r_GCGC'y
Cuts at: 586 1123 1371 2393 3372 586
Size: 537 248 1022 979 903
Fragments arranged by size:
1022 979 903 537 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 188 220 1531 1561 1563 1593 188
Size: 32 1311 30 2 30 2284
Fragments arranged by size:
2284 1311 32 30 30 2
HincII GTy'rAC
Cuts at: 728 728
Size: 3689
KasI G'GCGC_C
Cuts at: 1119 1367 1119
Size: 248 3441
KpnI G_GTAC'C
Cuts at: 709 709
Size: 3689
MluI A'CGCG_T
Cuts at: 3122 3122
Size: 3689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2360 2544 2360
Size: 184 3505
MscI TGG'CCA
Cuts at: 1450 1450
Size: 3689
MslI CAynn'nnrTG
Cuts at: 442 1678 1805 442
Size: 1236 127 2326
Fragments arranged by size:
2326 1236 127
MspA1I CmG'CkG
Cuts at: 283 800 1114 1474 2487 2732 283
Size: 517 314 360 1013 245 1240
Fragments arranged by size:
1240 1013 517 360 314 245
NaeI GCC'GGC
Cuts at: 1870 1870
Size: 3689
NarI GG'CG_CC
Cuts at: 1120 1368 1120
Size: 248 3441
NcoI C'CATG_G
Cuts at: 539 539
Size: 3689
NgoMIV G'CCGG_C
Cuts at: 1868 1868
Size: 3689
NsiI A_TGCA'T
Cuts at: 189 189
Size: 3689
NspI r_CATG'y
Cuts at: 742 1242 2149 742
Size: 500 907 2282
Fragments arranged by size:
2282 907 500
PciI A'CATG_T
Cuts at: 2145 2145
Size: 3689
PfoI T'CCnGG_A
Cuts at: 192 192
Size: 3689
PmeI GTTT'AAAC
Cuts at: 688 688
Size: 3689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2828 2860 2828
Size: 32 3657
PstI C_TGCA'G
Cuts at: 736 1421 736
Size: 685 3004
PvuI CG_AT'CG
Cuts at: 104 485 104
Size: 381 3308
PvuII CAG'CTG
Cuts at: 283 1114 1474 283
Size: 831 360 2498
Fragments arranged by size:
2498 831 360
RsrII CG'GwC_CG
Cuts at: 1884 1884
Size: 3689
SacI G_AGCT'C
Cuts at: 703 703
Size: 3689
SalI G'TCGA_C
Cuts at: 726 726
Size: 3689
SapI GCTCTTCn'nnn_
Cuts at: 1712 1922 1712
Size: 210 3479
SbfI CC_TGCA'GG
Cuts at: 736 736
Size: 3689
ScaI AGT'ACT
Cuts at: 3206 3206
Size: 3689
SfcI C'TryA_G
Cuts at: 33 146 732 1417 2410 2601 33
Size: 113 586 685 993 191 1121
Fragments arranged by size:
1121 993 685 586 191 113
SfoI GGC'GCC
Cuts at: 1121 1369 1121
Size: 248 3441
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3689
SmaI CCC'GGG
Cuts at: 711 711
Size: 3689
SmlI C'TyrA_G
Cuts at: 601 844 1759 2251 2513 601
Size: 243 915 492 262 1777
Fragments arranged by size:
1777 915 492 262 243
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3689
SphI G_CATG'C
Cuts at: 742 1242 742
Size: 500 3189
StuI AGG'CCT
Cuts at: 599 599
Size: 3689
StyI C'CwwG_G
Cuts at: 539 823 1177 539
Size: 284 354 3051
Fragments arranged by size:
3051 354 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1272 1405 1864 2073 1272
Size: 133 459 209 2888
Fragments arranged by size:
2888 459 209 133
TatI w'GTAC_w
Cuts at: 3204 3204
Size: 3689
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 242 274 3206 3238 242
Size: 32 2932 32 693
Fragments arranged by size:
2932 693 32 32
Tth111I GACn'n_nGTC
Cuts at: 1486 1486
Size: 3689
XbaI T'CTAG_A
Cuts at: 720 720
Size: 3689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 123 441 1184 123
Size: 318 743 2628
Fragments arranged by size:
2628 743 318
XmaI C'CCGG_G
Cuts at: 709 709
Size: 3689
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BmgBI BmtI BsaXI
BseRI BsgI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI