Restriction map for
pF1KB8247
(Circular) (Six-base) MAPSORT of: pF1KB8247.seq Check: 1569 from: 1 to: 3884
pF1KB8247 3884 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 180 500 922 180
Size: 320 422 3142
Fragments arranged by size:
3142 422 320
Acc65I G'GTAC_C
Cuts at: 900 900
Size: 3884
AclI AA'CG_TT
Cuts at: 3724 3724
Size: 3884
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1709 2141 2888 3156 1709
Size: 432 747 268 2437
Fragments arranged by size:
2437 747 432 268
AfeI AGC'GCT
Cuts at: 325 325
Size: 3884
AflIII A'CryG_T
Cuts at: 1236 2340 3317 1236
Size: 1104 977 1803
Fragments arranged by size:
1803 1104 977
AgeI A'CCGG_T
Cuts at: 2272 2272
Size: 3884
AlwNI CAG_nnn'CTG
Cuts at: 1189 2756 1189
Size: 1567 2317
ApaI G_GGCC'C
Cuts at: 285 763 285
Size: 478 3406
ApaLI G'TGCA_C
Cuts at: 2654 2654
Size: 3884
ApoI r'AATT_y
Cuts at: 888 3237 3493 888
Size: 2349 256 1279
Fragments arranged by size:
2349 1279 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3884
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3884
AvaI C'yCGr_G
Cuts at: 376 904 376
Size: 528 3356
BamHI G'GATC_C
Cuts at: 695 909 695
Size: 214 3670
BanI G'GyrC_C
Cuts at: 900 1314 1562 1597 900
Size: 414 248 35 3187
Fragments arranged by size:
3187 414 248 35
BanII G_rGCy'C
Cuts at: 206 285 763 898 1928 206
Size: 79 478 135 1030 2162
Fragments arranged by size:
2162 1030 478 135 79
BbeI G_GCGC'C
Cuts at: 1318 1566 1318
Size: 248 3636
BbsI GAAGACnn'nnnn_
Cuts at: 812 812
Size: 3884
BciVI GTATCCnnnnn_n'
Cuts at: 1782 2543 3884 1782
Size: 761 1341 1782
Fragments arranged by size:
1782 1341 761
BclI T'GATC_A
Cuts at: 1404 1404
Size: 3884
BglII A'GATC_T
Cuts at: 1399 3436 1399
Size: 2037 1847
BlpI GC'TnA_GC
Cuts at: 996 996
Size: 3884
Bme1580I G_kGCm'C
Cuts at: 285 763 1509 1602 2658 285
Size: 478 746 93 1056 1511
Fragments arranged by size:
1511 1056 746 478 93
BmrI ACTGGGnnnn_n'
Cuts at: 508 1096 1177 1512 3656 508
Size: 588 81 335 2144 736
Fragments arranged by size:
2144 736 588 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 3884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 292 544 886 220
Size: 72 252 342 3218
Fragments arranged by size:
3218 342 252 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 106 169 1060 1939 2431 2729 106
Size: 63 891 879 492 298 1261
Fragments arranged by size:
1261 891 879 492 298 63
BsaI GGTCTCn'nnnn_
Cuts at: 3588 3588
Size: 3884
BsaAI yAC'GTr
Cuts at: 1237 1237
Size: 3884
BsaBI GATnn'nnATC
Cuts at: 6 1398 3435 6
Size: 1392 2037 455
Fragments arranged by size:
2037 1392 455
BsaHI Gr'CG_yC
Cuts at: 1315 1563 2265 3761 1315
Size: 248 702 1496 1438
Fragments arranged by size:
1496 1438 702 248
BsaWI w'CCGG_w
Cuts at: 1073 1594 2272 2546 2693 3104 1073
Size: 521 678 274 147 411 1853
Fragments arranged by size:
1853 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 298 328 298
Size: 30 3854
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 406 406
Size: 3884
BseYI C'CCAG_C
Cuts at: 174 335 643 1309 2067 2644 3140 174
Size: 161 308 666 758 577 496 918
Fragments arranged by size:
918 758 666 577 496 308 161
BsiEI CG_ry'CG
Cuts at: 104 1426 1472 2680 104
Size: 1322 46 1208 1308
Fragments arranged by size:
1322 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 206 898 1676 1866 2658 206
Size: 692 778 190 792 1432
Fragments arranged by size:
1432 792 778 692 190
Bsp1286I G_dGCh'C
Cuts at: 206 285 763 898 1509 1602 1676 1866 1928
Size: 79 478 135 611 93 74 190 62
Cuts at: 1928 2658 206
Size: 730 1432
Fragments arranged by size:
1432 730 611 478 190 135 93 79
74 62
BspEI T'CCGG_A
Cuts at: 3104 3104
Size: 3884
BspHI T'CATG_A
Cuts at: 3060 3060
Size: 3884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 934 1148 1450 1831 934
Size: 214 302 381 2987
Fragments arranged by size:
2987 381 302 214
BsrBI CCG'CTC
Cuts at: 50 468 2176 2230 3714 3879 50
Size: 418 1708 54 1484 165 55
Fragments arranged by size:
1708 1484 418 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1796 1796
Size: 3884
BsrFI r'CCGG_y
Cuts at: 1882 2063 2272 1882
Size: 181 209 3494
Fragments arranged by size:
3494 209 181
BssSI C'ACGA_G
Cuts at: 2155 2513 2155
Size: 358 3526
BstAPI GCAn_nnn'nTGC
Cuts at: 1261 1261
Size: 3884
BstBI TT'CG_AA
Cuts at: 2245 2245
Size: 3884
BstXI CCAn_nnnn'nTGG
Cuts at: 831 831
Size: 3884
BstYI r'GATC_y
Cuts at: 1 695 909 1376 1399 1734 1980 2992 3078
Size: 694 214 467 23 335 246 1012 86
Cuts at: 3078 3090 3436 1
Size: 12 346 449
Fragments arranged by size:
1012 694 467 449 346 335 246 214
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1834 2015 2178 1834
Size: 181 163 3540
Fragments arranged by size:
3540 181 163
BtsI GCAGTG_nn'
Cuts at: 1127 1127
Size: 3884
DraI TTT'AAA
Cuts at: 883 883
Size: 3884
DraIII CAC_nnn'GTG
Cuts at: 516 516
Size: 3884
DrdI GACnn_nn'nnGTC
Cuts at: 1590 2448 1590
Size: 858 3026
EaeI y'GGCC_r
Cuts at: 566 833 1469 1643 2034 2061 3406 566
Size: 267 636 174 391 27 1345 1044
Fragments arranged by size:
1345 1044 636 391 267 174 27
EagI C'GGCC_G
Cuts at: 1469 1469
Size: 3884
EarI CTCTTCn'nnn_
Cuts at: 164 424 706 1907 2117 164
Size: 260 282 1201 210 1931
Fragments arranged by size:
1931 1201 282 260 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2092 2402 2548 3694 2092
Size: 310 146 1146 2282
Fragments arranged by size:
2282 1146 310 146
EcoICRI GAG'CTC
Cuts at: 204 896 204
Size: 692 3192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 292 544 886 1709 2141 2888 3156 220
Size: 72 252 342 823 432 747 268 948
Fragments arranged by size:
948 823 747 432 342 268 252 72
EcoNI CCTnn'n_nnAGG
Cuts at: 301 538 568 301
Size: 237 30 3617
Fragments arranged by size:
3617 237 30
EcoO109I rG'GnC_Cy
Cuts at: 281 753 760 1023 281
Size: 472 7 263 3142
Fragments arranged by size:
3142 472 263 7
EcoRI G'AATT_C
Cuts at: 888 888
Size: 3884
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1903 1903
Size: 3884
FspI TGC'GCA
Cuts at: 1665 1665
Size: 3884
HaeII r_GCGC'y
Cuts at: 327 1318 1566 2588 3567 327
Size: 991 248 1022 979 644
Fragments arranged by size:
1022 991 979 644 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 298 330 737 769 1726 1756 1758 1788 298
Size: 32 407 32 957 30 2 30 2394
Fragments arranged by size:
2394 957 407 32 32 30 30 2
HincII GTy'rAC
Cuts at: 181 923 181
Size: 742 3142
KasI G'GCGC_C
Cuts at: 1314 1562 1314
Size: 248 3636
KpnI G_GTAC'C
Cuts at: 904 904
Size: 3884
MluI A'CGCG_T
Cuts at: 3317 3317
Size: 3884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 443 2555 2739 443
Size: 2112 184 1588
Fragments arranged by size:
2112 1588 184
MscI TGG'CCA
Cuts at: 568 835 1645 568
Size: 267 810 2807
Fragments arranged by size:
2807 810 267
MslI CAynn'nnrTG
Cuts at: 1873 2000 1873
Size: 127 3757
MspA1I CmG'CkG
Cuts at: 864 995 1309 1669 2682 2927 864
Size: 131 314 360 1013 245 1821
Fragments arranged by size:
1821 1013 360 314 245 131
NaeI GCC'GGC
Cuts at: 2065 2065
Size: 3884
NarI GG'CG_CC
Cuts at: 1315 1563 1315
Size: 248 3636
NgoMIV G'CCGG_C
Cuts at: 2063 2063
Size: 3884
NspI r_CATG'y
Cuts at: 937 1437 2344 937
Size: 500 907 2477
Fragments arranged by size:
2477 907 500
PciI A'CATG_T
Cuts at: 2340 2340
Size: 3884
PflMI CCAn_nnn'nTGG
Cuts at: 402 402
Size: 3884
PmeI GTTT'AAAC
Cuts at: 883 883
Size: 3884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3023 3055 3023
Size: 32 3852
PpuMI rG'GwC_Cy
Cuts at: 753 753
Size: 3884
PshAI GACnn'nnGTC
Cuts at: 496 496
Size: 3884
PspOMI G'GGCC_C
Cuts at: 281 759 281
Size: 478 3406
PstI C_TGCA'G
Cuts at: 931 1616 931
Size: 685 3199
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3884
PvuII CAG'CTG
Cuts at: 864 1309 1669 864
Size: 445 360 3079
Fragments arranged by size:
3079 445 360
RsrII CG'GwC_CG
Cuts at: 2079 2079
Size: 3884
SacI G_AGCT'C
Cuts at: 206 898 206
Size: 692 3192
SalI G'TCGA_C
Cuts at: 179 921 179
Size: 742 3142
SapI GCTCTTCn'nnn_
Cuts at: 164 1907 2117 164
Size: 1743 210 1931
Fragments arranged by size:
1931 1743 210
SbfI CC_TGCA'GG
Cuts at: 931 931
Size: 3884
ScaI AGT'ACT
Cuts at: 3401 3401
Size: 3884
SfcI C'TryA_G
Cuts at: 33 774 927 1612 2605 2796 33
Size: 741 153 685 993 191 1121
Fragments arranged by size:
1121 993 741 685 191 153
SfoI GGC'GCC
Cuts at: 1316 1564 1316
Size: 248 3636
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3884
SmaI CCC'GGG
Cuts at: 906 906
Size: 3884
SmlI C'TyrA_G
Cuts at: 121 184 1039 1954 2446 2708 121
Size: 63 855 915 492 262 1297
Fragments arranged by size:
1297 915 855 492 262 63
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3884
SphI G_CATG'C
Cuts at: 937 1437 937
Size: 500 3384
StuI AGG'CCT
Cuts at: 661 661
Size: 3884
StyI C'CwwG_G
Cuts at: 479 569 1018 1372 479
Size: 90 449 354 2991
Fragments arranged by size:
2991 449 354 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 527 635 729 1467 1600 2059 2268 527
Size: 108 94 738 133 459 209 2143
Fragments arranged by size:
2143 738 459 209 133 108 94
TatI w'GTAC_w
Cuts at: 3399 3399
Size: 3884
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 502 534 801 833 3401 3433 502
Size: 32 267 32 2568 32 953
Fragments arranged by size:
2568 953 267 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1681 1681
Size: 3884
XbaI T'CTAG_A
Cuts at: 915 915
Size: 3884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1379 1379
Size: 3884
XmaI C'CCGG_G
Cuts at: 904 904
Size: 3884
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I BtgI ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI