Restriction map for
pF1KB8246
(Circular) (Six-base) MAPSORT of: pF1KB8246.seq Check: 4762 from: 1 to: 3848
pF1KB8246 3848 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 442 442
Size: 3848
AccI GT'mk_AC
Cuts at: 886 886
Size: 3848
Acc65I G'GTAC_C
Cuts at: 321 864 321
Size: 543 3305
AclI AA'CG_TT
Cuts at: 3688 3688
Size: 3848
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 504 552 1673 2105 2852 3120 504
Size: 48 1121 432 747 268 1232
Fragments arranged by size:
1232 1121 747 432 268 48
AflIII A'CryG_T
Cuts at: 257 1200 2304 3281 257
Size: 943 1104 977 824
Fragments arranged by size:
1104 977 943 824
AgeI A'CCGG_T
Cuts at: 2236 2236
Size: 3848
AlwNI CAG_nnn'CTG
Cuts at: 348 1153 2720 348
Size: 805 1567 1476
Fragments arranged by size:
1567 1476 805
ApaLI G'TGCA_C
Cuts at: 253 2618 253
Size: 2365 1483
ApoI r'AATT_y
Cuts at: 488 852 3201 3457 488
Size: 364 2349 256 879
Fragments arranged by size:
2349 879 364 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3848
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
AvaI C'yCGr_G
Cuts at: 868 868
Size: 3848
BamHI G'GATC_C
Cuts at: 873 873
Size: 3848
BanI G'GyrC_C
Cuts at: 321 864 1278 1526 1561 321
Size: 543 414 248 35 2608
Fragments arranged by size:
2608 543 414 248 35
BanII G_rGCy'C
Cuts at: 233 862 1892 233
Size: 629 1030 2189
Fragments arranged by size:
2189 1030 629
BbeI G_GCGC'C
Cuts at: 1282 1530 1282
Size: 248 3600
BbsI GAAGACnn'nnnn_
Cuts at: 510 558 617 762 510
Size: 48 59 145 3596
Fragments arranged by size:
3596 145 59 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 340 374 340
Size: 34 3814
BciVI GTATCCnnnnn_n'
Cuts at: 1746 2507 3848 1746
Size: 761 1341 1746
Fragments arranged by size:
1746 1341 761
BclI T'GATC_A
Cuts at: 1368 1368
Size: 3848
BglII A'GATC_T
Cuts at: 1363 3400 1363
Size: 2037 1811
BlpI GC'TnA_GC
Cuts at: 960 960
Size: 3848
Bme1580I G_kGCm'C
Cuts at: 257 1473 1566 2622 257
Size: 1216 93 1056 1483
Fragments arranged by size:
1483 1216 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1060 1141 1476 3620 1060
Size: 81 335 2144 1288
Fragments arranged by size:
2144 1288 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 426 645 2215 426
Size: 219 1570 2059
Fragments arranged by size:
2059 1570 219
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 1024 1903 2395 2693 490
Size: 534 879 492 298 1645
Fragments arranged by size:
1645 879 534 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 319 3552 319
Size: 3233 615
BsaAI yAC'GTr
Cuts at: 447 1201 447
Size: 754 3094
BsaBI GATnn'nnATC
Cuts at: 6 1362 3399 6
Size: 1356 2037 455
Fragments arranged by size:
2037 1356 455
BsaHI Gr'CG_yC
Cuts at: 1279 1527 2229 3725 1279
Size: 248 702 1496 1402
Fragments arranged by size:
1496 1402 702 248
BsaWI w'CCGG_w
Cuts at: 1037 1558 2236 2510 2657 3068 1037
Size: 521 678 274 147 411 1817
Fragments arranged by size:
1817 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 572 602 572
Size: 30 3818
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 415 415
Size: 3848
BseYI C'CCAG_C
Cuts at: 691 788 1273 2031 2608 3104 691
Size: 97 485 758 577 496 1435
Fragments arranged by size:
1435 758 577 496 485 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 403
Size: 3848
BsiEI CG_ry'CG
Cuts at: 104 191 1390 1436 2644 104
Size: 87 1199 46 1208 1308
Fragments arranged by size:
1308 1208 1199 87 46
BsiHKAI G_wGCw'C
Cuts at: 233 257 506 862 1640 1830 2622 233
Size: 24 249 356 778 190 792 1459
Fragments arranged by size:
1459 792 778 356 249 190 24
Bsp1286I G_dGCh'C
Cuts at: 233 257 506 862 1473 1566 1640 1830 1892
Size: 24 249 356 611 93 74 190 62
Cuts at: 1892 2622 233
Size: 730 1459
Fragments arranged by size:
1459 730 611 356 249 190 93 74
62 24
BspEI T'CCGG_A
Cuts at: 3068 3068
Size: 3848
BspHI T'CATG_A
Cuts at: 3024 3024
Size: 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 442 898 1112 1414 1795 442
Size: 456 214 302 381 2495
Fragments arranged by size:
2495 456 381 302 214
BsrBI CCG'CTC
Cuts at: 50 405 2140 2194 3678 3843 50
Size: 355 1735 54 1484 165 55
Fragments arranged by size:
1735 1484 355 165 55 54
BsrDI GCAATG_nn'
Cuts at: 429 444 800 1760 429
Size: 15 356 960 2517
Fragments arranged by size:
2517 960 356 15
BsrFI r'CCGG_y
Cuts at: 268 1846 2027 2236 268
Size: 1578 181 209 1880
Fragments arranged by size:
1880 1578 209 181
BssSI C'ACGA_G
Cuts at: 2119 2477 2119
Size: 358 3490
BstAPI GCAn_nnn'nTGC
Cuts at: 1225 1225
Size: 3848
BstBI TT'CG_AA
Cuts at: 2209 2209
Size: 3848
BstXI CCAn_nnnn'nTGG
Cuts at: 249 249
Size: 3848
BstYI r'GATC_y
Cuts at: 1 873 1340 1363 1698 1944 2956 3042 3054
Size: 872 467 23 335 246 1012 86 12
Cuts at: 3054 3400 1
Size: 346 449
Fragments arranged by size:
1012 872 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 248 107
Size: 141 3707
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1798 1979 2142 1798
Size: 181 163 3504
Fragments arranged by size:
3504 181 163
BtsI GCAGTG_nn'
Cuts at: 681 1091 681
Size: 410 3438
DraI TTT'AAA
Cuts at: 847 847
Size: 3848
DrdI GACnn_nn'nnGTC
Cuts at: 1554 2412 1554
Size: 858 2990
EaeI y'GGCC_r
Cuts at: 533 1433 1607 1998 2025 3370 533
Size: 900 174 391 27 1345 1011
Fragments arranged by size:
1345 1011 900 391 174 27
EagI C'GGCC_G
Cuts at: 1433 1433
Size: 3848
EarI CTCTTCn'nnn_
Cuts at: 638 1871 2081 638
Size: 1233 210 2405
Fragments arranged by size:
2405 1233 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2056 2366 2512 3658 2056
Size: 310 146 1146 2246
Fragments arranged by size:
2246 1146 310 146
EcoICRI GAG'CTC
Cuts at: 231 860 231
Size: 629 3219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 504 552 1673 2105 2852 3120 504
Size: 48 1121 432 747 268 1232
Fragments arranged by size:
1232 1121 747 432 268 48
EcoO109I rG'GnC_Cy
Cuts at: 987 987
Size: 3848
EcoRI G'AATT_C
Cuts at: 852 852
Size: 3848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 664 1867 664
Size: 1203 2645
FspI TGC'GCA
Cuts at: 1629 1629
Size: 3848
HaeII r_GCGC'y
Cuts at: 1282 1530 2552 3531 1282
Size: 248 1022 979 1599
Fragments arranged by size:
1599 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 610 642 1690 1720 1722 1752 610
Size: 32 1048 30 2 30 2706
Fragments arranged by size:
2706 1048 32 30 30 2
HincII GTy'rAC
Cuts at: 887 887
Size: 3848
KasI G'GCGC_C
Cuts at: 1278 1526 1278
Size: 248 3600
KpnI G_GTAC'C
Cuts at: 325 868 325
Size: 543 3305
MfeI C'AATT_G
Cuts at: 605 605
Size: 3848
MluI A'CGCG_T
Cuts at: 257 3281 257
Size: 3024 824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2519 2703 2519
Size: 184 3664
MscI TGG'CCA
Cuts at: 1609 1609
Size: 3848
MslI CAynn'nnrTG
Cuts at: 247 295 811 1837 1964 247
Size: 48 516 1026 127 2131
Fragments arranged by size:
2131 1026 516 127 48
MspA1I CmG'CkG
Cuts at: 959 1273 1633 2646 2891 959
Size: 314 360 1013 245 1916
Fragments arranged by size:
1916 1013 360 314 245
NaeI GCC'GGC
Cuts at: 270 2029 270
Size: 1759 2089
NarI GG'CG_CC
Cuts at: 1279 1527 1279
Size: 248 3600
NcoI C'CATG_G
Cuts at: 107 107
Size: 3848
NgoMIV G'CCGG_C
Cuts at: 268 2027 268
Size: 1759 2089
NspI r_CATG'y
Cuts at: 294 816 901 1401 2308 294
Size: 522 85 500 907 1834
Fragments arranged by size:
1834 907 522 500 85
PciI A'CATG_T
Cuts at: 2304 2304
Size: 3848
PmeI GTTT'AAAC
Cuts at: 847 847
Size: 3848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2987 3019 2987
Size: 32 3816
PstI C_TGCA'G
Cuts at: 895 1580 895
Size: 685 3163
PvuI CG_AT'CG
Cuts at: 104 191 104
Size: 87 3761
PvuII CAG'CTG
Cuts at: 1273 1633 1273
Size: 360 3488
RsrII CG'GwC_CG
Cuts at: 114 2043 114
Size: 1929 1919
SacI G_AGCT'C
Cuts at: 233 862 233
Size: 629 3219
SalI G'TCGA_C
Cuts at: 885 885
Size: 3848
SapI GCTCTTCn'nnn_
Cuts at: 1871 2081 1871
Size: 210 3638
SbfI CC_TGCA'GG
Cuts at: 895 895
Size: 3848
ScaI AGT'ACT
Cuts at: 3365 3365
Size: 3848
SexAI A'CCwGG_T
Cuts at: 539 539
Size: 3848
SfcI C'TryA_G
Cuts at: 33 210 891 1576 2569 2760 33
Size: 177 681 685 993 191 1121
Fragments arranged by size:
1121 993 685 681 191 177
SfoI GGC'GCC
Cuts at: 1280 1528 1280
Size: 248 3600
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
SmaI CCC'GGG
Cuts at: 870 870
Size: 3848
SmlI C'TyrA_G
Cuts at: 505 1003 1918 2410 2672 505
Size: 498 915 492 262 1681
Fragments arranged by size:
1681 915 498 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3848
SphI G_CATG'C
Cuts at: 816 901 1401 816
Size: 85 500 3263
Fragments arranged by size:
3263 500 85
StyI C'CwwG_G
Cuts at: 107 416 452 982 1336 107
Size: 309 36 530 354 2619
Fragments arranged by size:
2619 530 354 309 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 476 1431 1564 2023 2232 476
Size: 955 133 459 209 2092
Fragments arranged by size:
2092 955 459 209 133
TatI w'GTAC_w
Cuts at: 3363 3363
Size: 3848
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 572 604 3365 3397 572
Size: 32 2761 32 1023
Fragments arranged by size:
2761 1023 32 32
Tth111I GACn'n_nGTC
Cuts at: 1645 1645
Size: 3848
XbaI T'CTAG_A
Cuts at: 879 879
Size: 3848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1343 1343
Size: 3848
XmaI C'CCGG_G
Cuts at: 868 868
Size: 3848
XmnI GAAnn'nnTTC
Cuts at: 488 488
Size: 3848
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI