Restriction map for
pF1KB8243
(Circular) (Six-base) MAPSORT of: pF1KB8243.seq Check: 6821 from: 1 to: 3761
pF1KB8243 3761 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 799 799
Size: 3761
Acc65I G'GTAC_C
Cuts at: 777 777
Size: 3761
AclI AA'CG_TT
Cuts at: 3601 3601
Size: 3761
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 1586 2018 2765 3033 523
Size: 1063 432 747 268 1251
Fragments arranged by size:
1251 1063 747 432 268
AflIII A'CryG_T
Cuts at: 1113 2217 3194 1113
Size: 1104 977 1680
Fragments arranged by size:
1680 1104 977
AgeI A'CCGG_T
Cuts at: 2149 2149
Size: 3761
AlwNI CAG_nnn'CTG
Cuts at: 1066 2633 1066
Size: 1567 2194
ApaLI G'TGCA_C
Cuts at: 2531 2531
Size: 3761
ApoI r'AATT_y
Cuts at: 208 319 631 765 3114 3370 208
Size: 111 312 134 2349 256 599
Fragments arranged by size:
2349 599 312 256 134 111
AseI AT'TA_AT
Cuts at: 20 20
Size: 3761
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3761
AvaI C'yCGr_G
Cuts at: 781 781
Size: 3761
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 424 457 424
Size: 33 3728
BamHI G'GATC_C
Cuts at: 786 786
Size: 3761
BanI G'GyrC_C
Cuts at: 777 1191 1439 1474 777
Size: 414 248 35 3064
Fragments arranged by size:
3064 414 248 35
BanII G_rGCy'C
Cuts at: 128 775 1805 128
Size: 647 1030 2084
Fragments arranged by size:
2084 1030 647
BbeI G_GCGC'C
Cuts at: 1195 1443 1195
Size: 248 3513
BbsI GAAGACnn'nnnn_
Cuts at: 510 510
Size: 3761
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 538 572 538
Size: 34 3727
BciVI GTATCCnnnnn_n'
Cuts at: 1659 2420 3761 1659
Size: 761 1341 1659
Fragments arranged by size:
1659 1341 761
BclI T'GATC_A
Cuts at: 1281 1281
Size: 3761
BglI GCCn_nnn'nGGC
Cuts at: 417 417
Size: 3761
BglII A'GATC_T
Cuts at: 426 1276 3313 426
Size: 850 2037 874
Fragments arranged by size:
2037 874 850
BlpI GC'TnA_GC
Cuts at: 873 873
Size: 3761
Bme1580I G_kGCm'C
Cuts at: 1386 1479 2535 1386
Size: 93 1056 2612
Fragments arranged by size:
2612 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 351 973 1054 1389 3533 351
Size: 622 81 335 2144 579
Fragments arranged by size:
2144 622 579 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2128 2128
Size: 3761
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 937 1816 2308 2606 937
Size: 879 492 298 2092
Fragments arranged by size:
2092 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 611 3465 611
Size: 2854 907
BsaAI yAC'GTr
Cuts at: 1114 1114
Size: 3761
BsaBI GATnn'nnATC
Cuts at: 6 1275 3312 6
Size: 1269 2037 455
Fragments arranged by size:
2037 1269 455
BsaHI Gr'CG_yC
Cuts at: 1192 1440 2142 3638 1192
Size: 248 702 1496 1315
Fragments arranged by size:
1496 1315 702 248
BsaWI w'CCGG_w
Cuts at: 950 1471 2149 2423 2570 2981 950
Size: 521 678 274 147 411 1730
Fragments arranged by size:
1730 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 678 1186 1944 2521 3017 678
Size: 508 758 577 496 1422
Fragments arranged by size:
1422 758 577 508 496
BsiEI CG_ry'CG
Cuts at: 104 1303 1349 2557 104
Size: 1199 46 1208 1308
Fragments arranged by size:
1308 1208 1199 46
BsiHKAI G_wGCw'C
Cuts at: 704 775 1553 1743 2535 704
Size: 71 778 190 792 1930
Fragments arranged by size:
1930 792 778 190 71
Bsp1286I G_dGCh'C
Cuts at: 128 704 775 1386 1479 1553 1743 1805 2535
Size: 576 71 611 93 74 190 62 730
Cuts at: 2535 128
Size: 1354
Fragments arranged by size:
1354 730 611 576 190 93 74 71
62
BspEI T'CCGG_A
Cuts at: 2981 2981
Size: 3761
BspHI T'CATG_A
Cuts at: 2937 2937
Size: 3761
BspMI ACCTGCnnnn'nnnn_
Cuts at: 811 1025 1327 1708 811
Size: 214 302 381 2864
Fragments arranged by size:
2864 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2053 2107 3591 3756 50
Size: 2003 54 1484 165 55
Fragments arranged by size:
2003 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1673 1673
Size: 3761
BsrFI r'CCGG_y
Cuts at: 307 1759 1940 2149 307
Size: 1452 181 209 1919
Fragments arranged by size:
1919 1452 209 181
BssSI C'ACGA_G
Cuts at: 2032 2390 2032
Size: 358 3403
BstAPI GCAn_nnn'nTGC
Cuts at: 1138 1138
Size: 3761
BstBI TT'CG_AA
Cuts at: 2122 2122
Size: 3761
BstYI r'GATC_y
Cuts at: 1 426 786 1253 1276 1611 1857 2869 2955
Size: 425 360 467 23 335 246 1012 86
Cuts at: 2955 2967 3313 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 425 360 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3761
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1711 1892 2055 1711
Size: 181 163 3417
Fragments arranged by size:
3417 181 163
BtsI GCAGTG_nn'
Cuts at: 1004 1004
Size: 3761
DraI TTT'AAA
Cuts at: 760 760
Size: 3761
DrdI GACnn_nn'nnGTC
Cuts at: 1467 2325 1467
Size: 858 2903
EaeI y'GGCC_r
Cuts at: 309 1346 1520 1911 1938 3283 309
Size: 1037 174 391 27 1345 787
Fragments arranged by size:
1345 1037 787 391 174 27
EagI C'GGCC_G
Cuts at: 1346 1346
Size: 3761
EarI CTCTTCn'nnn_
Cuts at: 1784 1994 1784
Size: 210 3551
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1969 2279 2425 3571 1969
Size: 310 146 1146 2159
Fragments arranged by size:
2159 1146 310 146
EcoICRI GAG'CTC
Cuts at: 773 773
Size: 3761
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 1586 2018 2765 3033 523
Size: 1063 432 747 268 1251
Fragments arranged by size:
1251 1063 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 277 900 277
Size: 623 3138
EcoRI G'AATT_C
Cuts at: 765 765
Size: 3761
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1780 1780
Size: 3761
FspI TGC'GCA
Cuts at: 116 1542 116
Size: 1426 2335
HaeII r_GCGC'y
Cuts at: 1195 1443 2465 3444 1195
Size: 248 1022 979 1512
Fragments arranged by size:
1512 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 370 402 714 746 1603 1633 1635 1665 370
Size: 32 312 32 857 30 2 30 2466
Fragments arranged by size:
2466 857 312 32 32 30 30 2
HincII GTy'rAC
Cuts at: 800 800
Size: 3761
KasI G'GCGC_C
Cuts at: 1191 1439 1191
Size: 248 3513
KpnI G_GTAC'C
Cuts at: 781 781
Size: 3761
MluI A'CGCG_T
Cuts at: 3194 3194
Size: 3761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2432 2616 2432
Size: 184 3577
MscI TGG'CCA
Cuts at: 1522 1522
Size: 3761
MslI CAynn'nnrTG
Cuts at: 370 1750 1877 370
Size: 1380 127 2254
Fragments arranged by size:
2254 1380 127
MspA1I CmG'CkG
Cuts at: 872 1186 1546 2559 2804 872
Size: 314 360 1013 245 1829
Fragments arranged by size:
1829 1013 360 314 245
NaeI GCC'GGC
Cuts at: 309 1942 309
Size: 1633 2128
NarI GG'CG_CC
Cuts at: 1192 1440 1192
Size: 248 3513
NcoI C'CATG_G
Cuts at: 107 107
Size: 3761
NgoMIV G'CCGG_C
Cuts at: 307 1940 307
Size: 1633 2128
NspI r_CATG'y
Cuts at: 814 1314 2221 814
Size: 500 907 2354
Fragments arranged by size:
2354 907 500
PciI A'CATG_T
Cuts at: 2217 2217
Size: 3761
PfoI T'CCnGG_A
Cuts at: 734 734
Size: 3761
PmeI GTTT'AAAC
Cuts at: 760 760
Size: 3761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2900 2932 2900
Size: 32 3729
PpuMI rG'GwC_Cy
Cuts at: 277 277
Size: 3761
PstI C_TGCA'G
Cuts at: 808 1493 808
Size: 685 3076
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3761
PvuII CAG'CTG
Cuts at: 1186 1546 1186
Size: 360 3401
RsrII CG'GwC_CG
Cuts at: 1956 1956
Size: 3761
SacI G_AGCT'C
Cuts at: 775 775
Size: 3761
SalI G'TCGA_C
Cuts at: 798 798
Size: 3761
SapI GCTCTTCn'nnn_
Cuts at: 1784 1994 1784
Size: 210 3551
SbfI CC_TGCA'GG
Cuts at: 808 808
Size: 3761
ScaI AGT'ACT
Cuts at: 544 3278 544
Size: 2734 1027
SexAI A'CCwGG_T
Cuts at: 752 752
Size: 3761
SfcI C'TryA_G
Cuts at: 33 804 1489 2482 2673 33
Size: 771 685 993 191 1121
Fragments arranged by size:
1121 993 771 685 191
SfoI GGC'GCC
Cuts at: 1193 1441 1193
Size: 248 3513
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3761
SmaI CCC'GGG
Cuts at: 783 783
Size: 3761
SmlI C'TyrA_G
Cuts at: 916 1831 2323 2585 916
Size: 915 492 262 2092
Fragments arranged by size:
2092 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3761
SphI G_CATG'C
Cuts at: 814 1314 814
Size: 500 3261
StyI C'CwwG_G
Cuts at: 107 233 895 1249 107
Size: 126 662 354 2619
Fragments arranged by size:
2619 662 354 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 226 1344 1477 1936 2145 226
Size: 1118 133 459 209 1842
Fragments arranged by size:
1842 1118 459 209 133
TatI w'GTAC_w
Cuts at: 542 3276 542
Size: 2734 1027
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3278 3310 3278
Size: 32 3729
Tth111I GACn'n_nGTC
Cuts at: 1558 1558
Size: 3761
XbaI T'CTAG_A
Cuts at: 792 792
Size: 3761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1256 1256
Size: 3761
XmaI C'CCGG_G
Cuts at: 781 781
Size: 3761
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BfrBI BmgBI BmtI BpmI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI