Restriction map for
pF1KB8231
(Circular) (Six-base) MAPSORT of: pF1KB8231.seq Check: 8826 from: 1 to: 3746
pF1KB8231 3746 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 784 784
Size: 3746
Acc65I G'GTAC_C
Cuts at: 443 762 443
Size: 319 3427
AclI AA'CG_TT
Cuts at: 3586 3586
Size: 3746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 1571 2003 2750 3018 337
Size: 1234 432 747 268 1065
Fragments arranged by size:
1234 1065 747 432 268
AflIII A'CryG_T
Cuts at: 1098 2202 3179 1098
Size: 1104 977 1665
Fragments arranged by size:
1665 1104 977
AgeI A'CCGG_T
Cuts at: 2134 2134
Size: 3746
AlwNI CAG_nnn'CTG
Cuts at: 234 678 1051 2618 234
Size: 444 373 1567 1362
Fragments arranged by size:
1567 1362 444 373
ApaI G_GGCC'C
Cuts at: 319 319
Size: 3746
ApaLI G'TGCA_C
Cuts at: 2516 2516
Size: 3746
ApoI r'AATT_y
Cuts at: 750 3099 3355 750
Size: 2349 256 1141
Fragments arranged by size:
2349 1141 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3746
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
AvaI C'yCGr_G
Cuts at: 275 766 275
Size: 491 3255
BamHI G'GATC_C
Cuts at: 732 771 732
Size: 39 3707
BanI G'GyrC_C
Cuts at: 443 762 1176 1424 1459 443
Size: 319 414 248 35 2730
Fragments arranged by size:
2730 414 319 248 35
BanII G_rGCy'C
Cuts at: 319 342 760 1790 319
Size: 23 418 1030 2275
Fragments arranged by size:
2275 1030 418 23
BbeI G_GCGC'C
Cuts at: 1180 1428 1180
Size: 248 3498
BciVI GTATCCnnnnn_n'
Cuts at: 1644 2405 3746 1644
Size: 761 1341 1644
Fragments arranged by size:
1644 1341 761
BclI T'GATC_A
Cuts at: 1266 1266
Size: 3746
BglII A'GATC_T
Cuts at: 1261 3298 1261
Size: 2037 1709
BlpI GC'TnA_GC
Cuts at: 858 858
Size: 3746
Bme1580I G_kGCm'C
Cuts at: 319 1371 1464 2520 319
Size: 1052 93 1056 1545
Fragments arranged by size:
1545 1056 1052 93
BmrI ACTGGGnnnn_n'
Cuts at: 958 1039 1374 3518 958
Size: 81 335 2144 1186
Fragments arranged by size:
2144 1186 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 3746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 99 158 425 99
Size: 59 267 3420
Fragments arranged by size:
3420 267 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1801 2293 2591 922
Size: 879 492 298 2077
Fragments arranged by size:
2077 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 117 129 216 3450 117
Size: 12 87 3234 413
Fragments arranged by size:
3234 413 87 12
BsaAI yAC'GTr
Cuts at: 1099 1099
Size: 3746
BsaBI GATnn'nnATC
Cuts at: 6 731 1260 3297 6
Size: 725 529 2037 455
Fragments arranged by size:
2037 725 529 455
BsaHI Gr'CG_yC
Cuts at: 1177 1425 2127 3623 1177
Size: 248 702 1496 1300
Fragments arranged by size:
1496 1300 702 248
BsaWI w'CCGG_w
Cuts at: 561 935 1456 2134 2408 2555 2966 561
Size: 374 521 678 274 147 411 1341
Fragments arranged by size:
1341 678 521 411 374 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 641 660 641
Size: 19 3727
BseYI C'CCAG_C
Cuts at: 1171 1929 2506 3002 1171
Size: 758 577 496 1915
Fragments arranged by size:
1915 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1288 1334 2542 104
Size: 1184 46 1208 1308
Fragments arranged by size:
1308 1208 1184 46
BsiHKAI G_wGCw'C
Cuts at: 342 760 1538 1728 2520 342
Size: 418 778 190 792 1568
Fragments arranged by size:
1568 792 778 418 190
BsmBI CGTCTCn'nnnn_
Cuts at: 627 627
Size: 3746
Bsp1286I G_dGCh'C
Cuts at: 319 342 760 1371 1464 1538 1728 1790 2520
Size: 23 418 611 93 74 190 62 730
Cuts at: 2520 319
Size: 1545
Fragments arranged by size:
1545 730 611 418 190 93 74 62
23
BspEI T'CCGG_A
Cuts at: 561 2966 561
Size: 2405 1341
BspHI T'CATG_A
Cuts at: 342 2922 342
Size: 2580 1166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 796 1010 1312 1693 796
Size: 214 302 381 2849
Fragments arranged by size:
2849 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2038 2092 3576 3741 50
Size: 1988 54 1484 165 55
Fragments arranged by size:
1988 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 263 1658 263
Size: 1395 2351
BsrFI r'CCGG_y
Cuts at: 1744 1925 2134 1744
Size: 181 209 3356
Fragments arranged by size:
3356 209 181
BssSI C'ACGA_G
Cuts at: 2017 2375 2017
Size: 358 3388
BstAPI GCAn_nnn'nTGC
Cuts at: 1123 1123
Size: 3746
BstBI TT'CG_AA
Cuts at: 2107 2107
Size: 3746
BstXI CCAn_nnnn'nTGG
Cuts at: 390 390
Size: 3746
BstYI r'GATC_y
Cuts at: 1 732 771 1238 1261 1596 1842 2854 2940
Size: 731 39 467 23 335 246 1012 86
Cuts at: 2940 2952 3298 1
Size: 12 346 449
Fragments arranged by size:
1012 731 467 449 346 335 246 86
39 23 12
Bsu36I CC'TnA_GG
Cuts at: 419 419
Size: 3746
BtgI C'CryG_G
Cuts at: 107 107
Size: 3746
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1696 1877 2040 1696
Size: 181 163 3402
Fragments arranged by size:
3402 181 163
BtsI GCAGTG_nn'
Cuts at: 512 989 512
Size: 477 3269
DraI TTT'AAA
Cuts at: 745 745
Size: 3746
DrdI GACnn_nn'nnGTC
Cuts at: 1452 2310 1452
Size: 858 2888
EaeI y'GGCC_r
Cuts at: 1331 1505 1896 1923 3268 1331
Size: 174 391 27 1345 1809
Fragments arranged by size:
1809 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1331 1331
Size: 3746
EarI CTCTTCn'nnn_
Cuts at: 1769 1979 1769
Size: 210 3536
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1954 2264 2410 3556 1954
Size: 310 146 1146 2144
Fragments arranged by size:
2144 1146 310 146
EcoICRI GAG'CTC
Cuts at: 340 758 340
Size: 418 3328
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 99 158 337 425 1571 2003 2750 3018 99
Size: 59 179 88 1146 432 747 268 827
Fragments arranged by size:
1146 827 747 432 268 179 88 59
EcoO109I rG'GnC_Cy
Cuts at: 272 316 364 885 272
Size: 44 48 521 3133
Fragments arranged by size:
3133 521 48 44
EcoRI G'AATT_C
Cuts at: 750 750
Size: 3746
EcoRV GAT'ATC
Cuts at: 225 225
Size: 3746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1765 1765
Size: 3746
FspI TGC'GCA
Cuts at: 1527 1527
Size: 3746
HaeII r_GCGC'y
Cuts at: 1180 1428 2450 3429 1180
Size: 248 1022 979 1497
Fragments arranged by size:
1497 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 196 228 639 671 1588 1618 1620 1650 196
Size: 32 411 32 917 30 2 30 2292
Fragments arranged by size:
2292 917 411 32 32 30 30 2
HincII GTy'rAC
Cuts at: 401 785 401
Size: 384 3362
KasI G'GCGC_C
Cuts at: 1176 1424 1176
Size: 248 3498
KpnI G_GTAC'C
Cuts at: 447 766 447
Size: 319 3427
MfeI C'AATT_G
Cuts at: 256 256
Size: 3746
MluI A'CGCG_T
Cuts at: 3179 3179
Size: 3746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 453 2417 2601 453
Size: 1964 184 1598
Fragments arranged by size:
1964 1598 184
MscI TGG'CCA
Cuts at: 1507 1507
Size: 3746
MslI CAynn'nnrTG
Cuts at: 165 1735 1862 165
Size: 1570 127 2049
Fragments arranged by size:
2049 1570 127
MspA1I CmG'CkG
Cuts at: 857 1171 1531 2544 2789 857
Size: 314 360 1013 245 1814
Fragments arranged by size:
1814 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1927 1927
Size: 3746
NarI GG'CG_CC
Cuts at: 1177 1425 1177
Size: 248 3498
NcoI C'CATG_G
Cuts at: 107 107
Size: 3746
NgoMIV G'CCGG_C
Cuts at: 1925 1925
Size: 3746
NspI r_CATG'y
Cuts at: 799 1299 2206 799
Size: 500 907 2339
Fragments arranged by size:
2339 907 500
PciI A'CATG_T
Cuts at: 2202 2202
Size: 3746
PfoI T'CCnGG_A
Cuts at: 115 115
Size: 3746
PmeI GTTT'AAAC
Cuts at: 745 745
Size: 3746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2885 2917 2885
Size: 32 3714
PpuMI rG'GwC_Cy
Cuts at: 364 364
Size: 3746
PspOMI G'GGCC_C
Cuts at: 315 315
Size: 3746
PstI C_TGCA'G
Cuts at: 519 793 1478 519
Size: 274 685 2787
Fragments arranged by size:
2787 685 274
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3746
PvuII CAG'CTG
Cuts at: 1171 1531 1171
Size: 360 3386
RsrII CG'GwC_CG
Cuts at: 1941 1941
Size: 3746
SacI G_AGCT'C
Cuts at: 342 760 342
Size: 418 3328
SalI G'TCGA_C
Cuts at: 783 783
Size: 3746
SapI GCTCTTCn'nnn_
Cuts at: 1769 1979 1769
Size: 210 3536
SbfI CC_TGCA'GG
Cuts at: 793 793
Size: 3746
ScaI AGT'ACT
Cuts at: 3263 3263
Size: 3746
SfcI C'TryA_G
Cuts at: 33 515 789 1474 2467 2658 33
Size: 482 274 685 993 191 1121
Fragments arranged by size:
1121 993 685 482 274 191
SfoI GGC'GCC
Cuts at: 1178 1426 1178
Size: 248 3498
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
SmaI CCC'GGG
Cuts at: 768 768
Size: 3746
SmlI C'TyrA_G
Cuts at: 901 1816 2308 2570 901
Size: 915 492 262 2077
Fragments arranged by size:
2077 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3746
SphI G_CATG'C
Cuts at: 799 1299 799
Size: 500 3246
StyI C'CwwG_G
Cuts at: 107 880 1234 107
Size: 773 354 2619
Fragments arranged by size:
2619 773 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1329 1462 1921 2130 1329
Size: 133 459 209 2945
Fragments arranged by size:
2945 459 209 133
TatI w'GTAC_w
Cuts at: 3261 3261
Size: 3746
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 3714
Tth111I GACn'n_nGTC
Cuts at: 208 1543 208
Size: 1335 2411
XbaI T'CTAG_A
Cuts at: 495 777 495
Size: 282 3464
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 276 309 1241 276
Size: 33 932 2781
Fragments arranged by size:
2781 932 33
XmaI C'CCGG_G
Cuts at: 766 766
Size: 3746
XmnI GAAnn'nnTTC
Cuts at: 350 350
Size: 3746
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI