Restriction map for
pF1KB8221
(Circular) (Six-base) MAPSORT of: pF1KB8221.seq Check: 9816 from: 1 to: 3857
pF1KB8221 3857 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 895 895
Size: 3857
Acc65I G'GTAC_C
Cuts at: 873 873
Size: 3857
AclI AA'CG_TT
Cuts at: 3697 3697
Size: 3857
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1682 2114 2861 3129 1682
Size: 432 747 268 2410
Fragments arranged by size:
2410 747 432 268
AflIII A'CryG_T
Cuts at: 1209 2313 3290 1209
Size: 1104 977 1776
Fragments arranged by size:
1776 1104 977
AgeI A'CCGG_T
Cuts at: 2245 2245
Size: 3857
AhdI GACnn_n'nnGTC
Cuts at: 720 720
Size: 3857
AlwNI CAG_nnn'CTG
Cuts at: 427 733 1162 2729 427
Size: 306 429 1567 1555
Fragments arranged by size:
1567 1555 429 306
ApaI G_GGCC'C
Cuts at: 293 293
Size: 3857
ApaLI G'TGCA_C
Cuts at: 847 2627 847
Size: 1780 2077
ApoI r'AATT_y
Cuts at: 861 3210 3466 861
Size: 2349 256 1252
Fragments arranged by size:
2349 1252 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3857
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
AvaI C'yCGr_G
Cuts at: 286 877 286
Size: 591 3266
BamHI G'GATC_C
Cuts at: 120 882 120
Size: 762 3095
BanI G'GyrC_C
Cuts at: 130 142 181 280 873 1287 1535 1570 130
Size: 12 39 99 593 414 248 35 2417
Fragments arranged by size:
2417 593 414 248 99 39 35 12
BanII G_rGCy'C
Cuts at: 293 871 1901 293
Size: 578 1030 2249
Fragments arranged by size:
2249 1030 578
BbeI G_GCGC'C
Cuts at: 134 146 185 284 1291 1539 134
Size: 12 39 99 1007 248 2452
Fragments arranged by size:
2452 1007 248 99 39 12
BbsI GAAGACnn'nnnn_
Cuts at: 200 200
Size: 3857
BciVI GTATCCnnnnn_n'
Cuts at: 445 1755 2516 3857 445
Size: 1310 761 1341 445
Fragments arranged by size:
1341 1310 761 445
BclI T'GATC_A
Cuts at: 1377 1377
Size: 3857
BglI GCCn_nnn'nGGC
Cuts at: 214 214
Size: 3857
BglII A'GATC_T
Cuts at: 1372 3409 1372
Size: 2037 1820
BlpI GC'TnA_GC
Cuts at: 113 969 113
Size: 856 3001
Bme1580I G_kGCm'C
Cuts at: 293 851 1482 1575 2631 293
Size: 558 631 93 1056 1519
Fragments arranged by size:
1519 1056 631 558 93
BmgBI CAC'GTC
Cuts at: 633 633
Size: 3857
BmrI ACTGGGnnnn_n'
Cuts at: 1069 1150 1485 3629 1069
Size: 81 335 2144 1297
Fragments arranged by size:
2144 1297 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 3857
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1033 1912 2404 2702 814
Size: 219 879 492 298 1969
Fragments arranged by size:
1969 879 492 298 219
BsaI GGTCTCn'nnnn_
Cuts at: 3561 3561
Size: 3857
BsaAI yAC'GTr
Cuts at: 1210 1210
Size: 3857
BsaBI GATnn'nnATC
Cuts at: 6 1371 3408 6
Size: 1365 2037 455
Fragments arranged by size:
2037 1365 455
BsaHI Gr'CG_yC
Cuts at: 131 143 182 281 1288 1536 2238 3734 131
Size: 12 39 99 1007 248 702 1496 254
Fragments arranged by size:
1496 1007 702 254 248 99 39 12
BsaWI w'CCGG_w
Cuts at: 321 1046 1567 2245 2519 2666 3077 321
Size: 725 521 678 274 147 411 1101
Fragments arranged by size:
1101 725 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 162 293 1282 2040 2617 3113 162
Size: 131 989 758 577 496 906
Fragments arranged by size:
989 906 758 577 496 131
BsiEI CG_ry'CG
Cuts at: 104 1399 1445 2653 104
Size: 1295 46 1208 1308
Fragments arranged by size:
1308 1295 1208 46
BsiHKAI G_wGCw'C
Cuts at: 851 871 1649 1839 2631 851
Size: 20 778 190 792 2077
Fragments arranged by size:
2077 792 778 190 20
Bsp1286I G_dGCh'C
Cuts at: 293 851 871 1482 1575 1649 1839 1901 2631
Size: 558 20 611 93 74 190 62 730
Cuts at: 2631 293
Size: 1519
Fragments arranged by size:
1519 730 611 558 190 93 74 62
20
BspEI T'CCGG_A
Cuts at: 321 3077 321
Size: 2756 1101
BspHI T'CATG_A
Cuts at: 3033 3033
Size: 3857
BspMI ACCTGCnnnn'nnnn_
Cuts at: 907 1121 1423 1804 907
Size: 214 302 381 2960
Fragments arranged by size:
2960 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2149 2203 3687 3852 50
Size: 2099 54 1484 165 55
Fragments arranged by size:
2099 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1769 1769
Size: 3857
BsrFI r'CCGG_y
Cuts at: 139 178 184 1855 2036 2245 139
Size: 39 6 1671 181 209 1751
Fragments arranged by size:
1751 1671 209 181 39 6
BssSI C'ACGA_G
Cuts at: 2128 2486 2128
Size: 358 3499
BstAPI GCAn_nnn'nTGC
Cuts at: 1234 1234
Size: 3857
BstBI TT'CG_AA
Cuts at: 2218 2218
Size: 3857
BstYI r'GATC_y
Cuts at: 1 120 882 1349 1372 1707 1953 2965 3051
Size: 119 762 467 23 335 246 1012 86
Cuts at: 3051 3063 3409 1
Size: 12 346 449
Fragments arranged by size:
1012 762 467 449 346 335 246 119
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3857
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1807 1988 2151 1807
Size: 181 163 3513
Fragments arranged by size:
3513 181 163
BtsI GCAGTG_nn'
Cuts at: 1100 1100
Size: 3857
ClaI AT'CG_AT
Cuts at: 411 411
Size: 3857
DraI TTT'AAA
Cuts at: 856 856
Size: 3857
DraIII CAC_nnn'GTG
Cuts at: 737 737
Size: 3857
DrdI GACnn_nn'nnGTC
Cuts at: 1563 2421 1563
Size: 858 2999
EaeI y'GGCC_r
Cuts at: 176 1442 1616 2007 2034 3379 176
Size: 1266 174 391 27 1345 654
Fragments arranged by size:
1345 1266 654 391 174 27
EagI C'GGCC_G
Cuts at: 1442 1442
Size: 3857
EarI CTCTTCn'nnn_
Cuts at: 1880 2090 1880
Size: 210 3647
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2065 2375 2521 3667 2065
Size: 310 146 1146 2255
Fragments arranged by size:
2255 1146 310 146
EcoICRI GAG'CTC
Cuts at: 869 869
Size: 3857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1682 2114 2861 3129 1682
Size: 432 747 268 2410
Fragments arranged by size:
2410 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 679 832 679
Size: 153 3704
EcoO109I rG'GnC_Cy
Cuts at: 290 837 996 290
Size: 547 159 3151
Fragments arranged by size:
3151 547 159
EcoRI G'AATT_C
Cuts at: 861 861
Size: 3857
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1876 1876
Size: 3857
FspI TGC'GCA
Cuts at: 1638 1638
Size: 3857
HaeII r_GCGC'y
Cuts at: 134 146 185 284 1291 1539 2561 3540 134
Size: 12 39 99 1007 248 1022 979 451
Fragments arranged by size:
1022 1007 979 451 248 99 39 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 706 738 1699 1729 1731 1761 706
Size: 32 961 30 2 30 2802
Fragments arranged by size:
2802 961 32 30 30 2
HincII GTy'rAC
Cuts at: 896 896
Size: 3857
HindIII A'AGCT_T
Cuts at: 605 605
Size: 3857
KasI G'GCGC_C
Cuts at: 130 142 181 280 1287 1535 130
Size: 12 39 99 1007 248 2452
Fragments arranged by size:
2452 1007 248 99 39 12
KpnI G_GTAC'C
Cuts at: 877 877
Size: 3857
MluI A'CGCG_T
Cuts at: 3290 3290
Size: 3857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 400 2528 2712 400
Size: 2128 184 1545
Fragments arranged by size:
2128 1545 184
MscI TGG'CCA
Cuts at: 1618 1618
Size: 3857
MslI CAynn'nnrTG
Cuts at: 1846 1973 1846
Size: 127 3730
MspA1I CmG'CkG
Cuts at: 843 968 1282 1642 2655 2900 843
Size: 125 314 360 1013 245 1800
Fragments arranged by size:
1800 1013 360 314 245 125
NaeI GCC'GGC
Cuts at: 141 180 186 2038 141
Size: 39 6 1852 1960
Fragments arranged by size:
1960 1852 39 6
NarI GG'CG_CC
Cuts at: 131 143 182 281 1288 1536 131
Size: 12 39 99 1007 248 2452
Fragments arranged by size:
2452 1007 248 99 39 12
NcoI C'CATG_G
Cuts at: 107 107
Size: 3857
NgoMIV G'CCGG_C
Cuts at: 139 178 184 2036 139
Size: 39 6 1852 1960
Fragments arranged by size:
1960 1852 39 6
NspI r_CATG'y
Cuts at: 810 910 1410 2317 810
Size: 100 500 907 2350
Fragments arranged by size:
2350 907 500 100
PciI A'CATG_T
Cuts at: 2313 2313
Size: 3857
PfoI T'CCnGG_A
Cuts at: 269 269
Size: 3857
PmeI GTTT'AAAC
Cuts at: 856 856
Size: 3857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2996 3028 2996
Size: 32 3825
PspOMI G'GGCC_C
Cuts at: 289 289
Size: 3857
PstI C_TGCA'G
Cuts at: 904 1589 904
Size: 685 3172
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3857
PvuII CAG'CTG
Cuts at: 1282 1642 1282
Size: 360 3497
RsrII CG'GwC_CG
Cuts at: 2052 2052
Size: 3857
SacI G_AGCT'C
Cuts at: 871 871
Size: 3857
SalI G'TCGA_C
Cuts at: 894 894
Size: 3857
SapI GCTCTTCn'nnn_
Cuts at: 1880 2090 1880
Size: 210 3647
SbfI CC_TGCA'GG
Cuts at: 904 904
Size: 3857
ScaI AGT'ACT
Cuts at: 3374 3374
Size: 3857
SfcI C'TryA_G
Cuts at: 33 568 669 900 1585 2578 2769 33
Size: 535 101 231 685 993 191 1121
Fragments arranged by size:
1121 993 685 535 231 191 101
SfoI GGC'GCC
Cuts at: 132 144 183 282 1289 1537 132
Size: 12 39 99 1007 248 2452
Fragments arranged by size:
2452 1007 248 99 39 12
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
SgrAI Cr'CCGG_yG
Cuts at: 184 184
Size: 3857
SmaI CCC'GGG
Cuts at: 288 879 288
Size: 591 3266
SmlI C'TyrA_G
Cuts at: 829 1012 1927 2419 2681 829
Size: 183 915 492 262 2005
Fragments arranged by size:
2005 915 492 262 183
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3857
SphI G_CATG'C
Cuts at: 810 910 1410 810
Size: 100 500 3257
Fragments arranged by size:
3257 500 100
StuI AGG'CCT
Cuts at: 259 259
Size: 3857
StyI C'CwwG_G
Cuts at: 107 991 1345 107
Size: 884 354 2619
Fragments arranged by size:
2619 884 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1440 1573 2032 2241 1440
Size: 133 459 209 3056
Fragments arranged by size:
3056 459 209 133
TatI w'GTAC_w
Cuts at: 3372 3372
Size: 3857
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3374 3406 3374
Size: 32 3825
Tth111I GACn'n_nGTC
Cuts at: 1654 1654
Size: 3857
XbaI T'CTAG_A
Cuts at: 888 888
Size: 3857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 367 734 1352 114
Size: 253 367 618 2619
Fragments arranged by size:
2619 618 367 253
XmaI C'CCGG_G
Cuts at: 286 877 286
Size: 591 3266
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI BpmI Bpu10I
BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I CspCI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI