Restriction map for
pF1KB8220
(Circular) (Six-base) MAPSORT of: pF1KB8220.seq Check: 1946 from: 1 to: 4409
pF1KB8220 4409 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AatII G_ACGT'C
Cuts at: 452 452
Size: 4409
AccI GT'mk_AC
Cuts at: 452 1447 452
Size: 995 3414
Acc65I G'GTAC_C
Cuts at: 875 1425 875
Size: 550 3859
AclI AA'CG_TT
Cuts at: 4249 4249
Size: 4409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 2666 3413 3681 2234
Size: 432 747 268 2962
Fragments arranged by size:
2962 747 432 268
AflIII A'CryG_T
Cuts at: 1761 2865 3842 1761
Size: 1104 977 2328
Fragments arranged by size:
2328 1104 977
AgeI A'CCGG_T
Cuts at: 2797 2797
Size: 4409
AhdI GACnn_n'nnGTC
Cuts at: 763 996 1296 763
Size: 233 300 3876
Fragments arranged by size:
3876 300 233
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 685 717 685
Size: 32 4377
AlwNI CAG_nnn'CTG
Cuts at: 549 907 1161 1714 3281 549
Size: 358 254 553 1567 1677
Fragments arranged by size:
1677 1567 553 358 254
ApaLI G'TGCA_C
Cuts at: 3179 3179
Size: 4409
ApoI r'AATT_y
Cuts at: 1413 3762 4018 1413
Size: 2349 256 1804
Fragments arranged by size:
2349 1804 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4409
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4409
AvaI C'yCGr_G
Cuts at: 251 302 1429 251
Size: 51 1127 3231
Fragments arranged by size:
3231 1127 51
BamHI G'GATC_C
Cuts at: 1434 1434
Size: 4409
BanI G'GyrC_C
Cuts at: 208 875 965 1425 1839 2087 2122 208
Size: 667 90 460 414 248 35 2495
Fragments arranged by size:
2495 667 460 414 248 90 35
BanII G_rGCy'C
Cuts at: 335 434 1423 2453 335
Size: 99 989 1030 2291
Fragments arranged by size:
2291 1030 989 99
BbeI G_GCGC'C
Cuts at: 969 1843 2091 969
Size: 874 248 3287
Fragments arranged by size:
3287 874 248
BbsI GAAGACnn'nnnn_
Cuts at: 1345 1345
Size: 4409
BciVI GTATCCnnnnn_n'
Cuts at: 2307 3068 4409 2307
Size: 761 1341 2307
Fragments arranged by size:
2307 1341 761
BclI T'GATC_A
Cuts at: 1929 1929
Size: 4409
BfrBI ATG'CAT
Cuts at: 858 858
Size: 4409
BglI GCCn_nnn'nGGC
Cuts at: 649 649
Size: 4409
BglII A'GATC_T
Cuts at: 1924 3961 1924
Size: 2037 2372
BlpI GC'TnA_GC
Cuts at: 404 461 1521 404
Size: 57 1060 3292
Fragments arranged by size:
3292 1060 57
Bme1580I G_kGCm'C
Cuts at: 279 2034 2127 3183 279
Size: 1755 93 1056 1505
Fragments arranged by size:
1755 1505 1056 93
BmgBI CAC'GTC
Cuts at: 783 783
Size: 4409
BmrI ACTGGGnnnn_n'
Cuts at: 1621 1702 2037 4181 1621
Size: 81 335 2144 1849
Fragments arranged by size:
2144 1849 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2776 2776
Size: 4409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 556 685 706 724 1015 1045 1120 538
Size: 18 129 21 18 291 30 75 3827
Fragments arranged by size:
3827 291 129 75 30 21 18 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 726 1105 1303 1585 2464 2956 3254 726
Size: 379 198 282 879 492 298 1881
Fragments arranged by size:
1881 879 492 379 298 282 198
BsaI GGTCTCn'nnnn_
Cuts at: 292 4113 292
Size: 3821 588
BsaAI yAC'GTr
Cuts at: 1059 1762 1059
Size: 703 3706
BsaBI GATnn'nnATC
Cuts at: 6 1923 3960 6
Size: 1917 2037 455
Fragments arranged by size:
2037 1917 455
BsaHI Gr'CG_yC
Cuts at: 449 844 966 1840 2088 2790 4286 449
Size: 395 122 874 248 702 1496 572
Fragments arranged by size:
1496 874 702 572 395 248 122
BsaWI w'CCGG_w
Cuts at: 698 1232 1598 2119 2797 3071 3218 3629 698
Size: 534 366 521 678 274 147 411 1478
Fragments arranged by size:
1478 678 534 521 411 366 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 685 715 685
Size: 30 4379
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 129 144 703 706 736 739 1063 1240 129
Size: 15 559 3 30 3 324 177 3298
Fragments arranged by size:
3298 559 324 177 30 15 3 3
BseYI C'CCAG_C
Cuts at: 271 1834 2592 3169 3665 271
Size: 1563 758 577 496 1015
Fragments arranged by size:
1563 1015 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1270 1270
Size: 4409
BsiEI CG_ry'CG
Cuts at: 104 1951 1997 3205 104
Size: 1847 46 1208 1308
Fragments arranged by size:
1847 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 335 1391 1423 2201 2391 3183 335
Size: 1056 32 778 190 792 1561
Fragments arranged by size:
1561 1056 792 778 190 32
BsmI GAATG_Cn'
Cuts at: 236 236
Size: 4409
BsmBI CGTCTCn'nnnn_
Cuts at: 111 156 111
Size: 45 4364
Bsp1286I G_dGCh'C
Cuts at: 279 335 434 1391 1423 2034 2127 2201 2391
Size: 56 99 957 32 611 93 74 190
Cuts at: 2391 2453 3183 279
Size: 62 730 1505
Fragments arranged by size:
1505 957 730 611 190 99 93 74
62 56 32
BspEI T'CCGG_A
Cuts at: 3629 3629
Size: 4409
BspHI T'CATG_A
Cuts at: 3585 3585
Size: 4409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 753 994 1169 1274 1459 1673 1975 2356 753
Size: 241 175 105 185 214 302 381 2806
Fragments arranged by size:
2806 381 302 241 214 185 175 105
BsrBI CCG'CTC
Cuts at: 50 143 315 881 1053 2701 2755 4239 4404
Size: 93 172 566 172 1648 54 1484 165
Cuts at: 4404 50
Size: 55
Fragments arranged by size:
1648 1484 566 172 172 165 93 55
54
BsrDI GCAATG_nn'
Cuts at: 845 2321 845
Size: 1476 2933
BsrFI r'CCGG_y
Cuts at: 962 2407 2588 2797 962
Size: 1445 181 209 2574
Fragments arranged by size:
2574 1445 209 181
BssSI C'ACGA_G
Cuts at: 718 2680 3038 718
Size: 1962 358 2089
Fragments arranged by size:
2089 1962 358
BstAPI GCAn_nnn'nTGC
Cuts at: 283 1786 283
Size: 1503 2906
BstBI TT'CG_AA
Cuts at: 2770 2770
Size: 4409
BstXI CCAn_nnnn'nTGG
Cuts at: 441 441
Size: 4409
BstYI r'GATC_y
Cuts at: 1 660 695 713 818 1434 1901 1924 2259
Size: 659 35 18 105 616 467 23 335
Cuts at: 2259 2505 3517 3603 3615 3961 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 659 616 467 449 346 335 246
105 86 35 23 18 12
Bsu36I CC'TnA_GG
Cuts at: 156 589 156
Size: 433 3976
BtgI C'CryG_G
Cuts at: 107 1343 107
Size: 1236 3173
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2359 2540 2703 2359
Size: 181 163 4065
Fragments arranged by size:
4065 181 163
BtsI GCAGTG_nn'
Cuts at: 1652 1652
Size: 4409
DraI TTT'AAA
Cuts at: 1408 1408
Size: 4409
DrdI GACnn_nn'nnGTC
Cuts at: 2115 2973 2115
Size: 858 3551
EaeI y'GGCC_r
Cuts at: 189 551 785 1070 1994 2168 2559 2586 3931
Size: 362 234 285 924 174 391 27 1345
Cuts at: 3931 189
Size: 667
Fragments arranged by size:
1345 924 667 391 362 285 234 174
27
EagI C'GGCC_G
Cuts at: 1994 1994
Size: 4409
EarI CTCTTCn'nnn_
Cuts at: 863 1100 2432 2642 863
Size: 237 1332 210 2630
Fragments arranged by size:
2630 1332 237 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 922 2617 2927 3073 4219 922
Size: 1695 310 146 1146 1112
Fragments arranged by size:
1695 1146 1112 310 146
EcoICRI GAG'CTC
Cuts at: 333 1421 333
Size: 1088 3321
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 556 685 706 724 1015 1045 1120 2234
Size: 18 129 21 18 291 30 75 1114
Cuts at: 2234 2666 3413 3681 538
Size: 432 747 268 1266
Fragments arranged by size:
1266 1114 747 432 291 268 129 75
30 21 18 18
EcoO109I rG'GnC_Cy
Cuts at: 177 202 546 1158 1548 177
Size: 25 344 612 390 3038
Fragments arranged by size:
3038 612 390 344 25
EcoRI G'AATT_C
Cuts at: 1413 1413
Size: 4409
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 387 2428 387
Size: 2041 2368
FspI TGC'GCA
Cuts at: 2190 2190
Size: 4409
HaeII r_GCGC'y
Cuts at: 397 512 969 1090 1843 2091 3113 4092 397
Size: 115 457 121 753 248 1022 979 714
Fragments arranged by size:
1022 979 753 714 457 248 121 115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 519 551 749 781 982 1014 1282 1314 2251
Size: 32 198 32 201 32 268 32 937
Cuts at: 2251 2281 2283 2313 519
Size: 30 2 30 2615
Fragments arranged by size:
2615 937 268 201 198 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1448 1448
Size: 4409
KasI G'GCGC_C
Cuts at: 965 1839 2087 965
Size: 874 248 3287
Fragments arranged by size:
3287 874 248
KpnI G_GTAC'C
Cuts at: 879 1429 879
Size: 550 3859
MluI A'CGCG_T
Cuts at: 3842 3842
Size: 4409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1286 3080 3264 1286
Size: 1794 184 2431
Fragments arranged by size:
2431 1794 184
MscI TGG'CCA
Cuts at: 553 787 2170 553
Size: 234 1383 2792
Fragments arranged by size:
2792 1383 234
MslI CAynn'nnrTG
Cuts at: 1246 1333 1394 2398 2525 1246
Size: 87 61 1004 127 3130
Fragments arranged by size:
3130 1004 127 87 61
MspA1I CmG'CkG
Cuts at: 133 417 750 1520 1834 2194 3207 3452 133
Size: 284 333 770 314 360 1013 245 1090
Fragments arranged by size:
1090 1013 770 360 333 314 284 245
NaeI GCC'GGC
Cuts at: 2590 2590
Size: 4409
NarI GG'CG_CC
Cuts at: 966 1840 2088 966
Size: 874 248 3287
Fragments arranged by size:
3287 874 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4409
NgoMIV G'CCGG_C
Cuts at: 2588 2588
Size: 4409
NsiI A_TGCA'T
Cuts at: 860 860
Size: 4409
NspI r_CATG'y
Cuts at: 858 1462 1962 2869 858
Size: 604 500 907 2398
Fragments arranged by size:
2398 907 604 500
PciI A'CATG_T
Cuts at: 2865 2865
Size: 4409
PfoI T'CCnGG_A
Cuts at: 374 572 740 374
Size: 198 168 4043
Fragments arranged by size:
4043 198 168
PmeI GTTT'AAAC
Cuts at: 1408 1408
Size: 4409
PmlI CAC'GTG
Cuts at: 1059 1059
Size: 4409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 685 717 3548 3580 685
Size: 32 2831 32 1514
Fragments arranged by size:
2831 1514 32 32
PpuMI rG'GwC_Cy
Cuts at: 202 546 1158 202
Size: 344 612 3453
Fragments arranged by size:
3453 612 344
PstI C_TGCA'G
Cuts at: 1271 1456 2141 1271
Size: 185 685 3539
Fragments arranged by size:
3539 685 185
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4409
PvuII CAG'CTG
Cuts at: 417 750 1834 2194 417
Size: 333 1084 360 2632
Fragments arranged by size:
2632 1084 360 333
RsrII CG'GwC_CG
Cuts at: 2604 2604
Size: 4409
SacI G_AGCT'C
Cuts at: 335 1423 335
Size: 1088 3321
SalI G'TCGA_C
Cuts at: 1446 1446
Size: 4409
SapI GCTCTTCn'nnn_
Cuts at: 2432 2642 2432
Size: 210 4199
SbfI CC_TGCA'GG
Cuts at: 1456 1456
Size: 4409
ScaI AGT'ACT
Cuts at: 3926 3926
Size: 4409
SfcI C'TryA_G
Cuts at: 33 621 1203 1267 1452 2137 3130 3321 33
Size: 588 582 64 185 685 993 191 1121
Fragments arranged by size:
1121 993 685 588 582 191 185 64
SfoI GGC'GCC
Cuts at: 967 1841 2089 967
Size: 874 248 3287
Fragments arranged by size:
3287 874 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4409
SmaI CCC'GGG
Cuts at: 253 304 1431 253
Size: 51 1127 3231
Fragments arranged by size:
3231 1127 51
SmlI C'TyrA_G
Cuts at: 705 1120 1318 1564 2479 2971 3233 705
Size: 415 198 246 915 492 262 1881
Fragments arranged by size:
1881 915 492 415 262 246 198
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4409
SphI G_CATG'C
Cuts at: 1462 1962 1462
Size: 500 3909
StyI C'CwwG_G
Cuts at: 107 425 1543 1897 107
Size: 318 1118 354 2619
Fragments arranged by size:
2619 1118 354 318
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1992 2125 2584 2793 1992
Size: 133 459 209 3608
Fragments arranged by size:
3608 459 209 133
TatI w'GTAC_w
Cuts at: 3924 3924
Size: 4409
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3926 3958 3926
Size: 32 4377
Tth111I GACn'n_nGTC
Cuts at: 2206 2206
Size: 4409
XbaI T'CTAG_A
Cuts at: 1440 1440
Size: 4409
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 591 1904 591
Size: 1313 3096
XmaI C'CCGG_G
Cuts at: 251 302 1429 251
Size: 51 1127 3231
Fragments arranged by size:
3231 1127 51
ZraI GAC'GTC
Cuts at: 450 450
Size: 4409
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PfoI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BmtI Bpu10I BsiWI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PasI PflMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI