Restriction map for
pF1KB8219
(Circular) (Six-base) MAPSORT of: pF1KB8219.seq Check: 9188 from: 1 to: 4064
pF1KB8219 4064 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 669 669
Size: 4064
AccI GT'mk_AC
Cuts at: 1102 1102
Size: 4064
Acc65I G'GTAC_C
Cuts at: 1080 1080
Size: 4064
AclI AA'CG_TT
Cuts at: 3904 3904
Size: 4064
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 731 1889 2321 3068 3336 731
Size: 1158 432 747 268 1459
Fragments arranged by size:
1459 1158 747 432 268
AflII C'TTAA_G
Cuts at: 151 151
Size: 4064
AflIII A'CryG_T
Cuts at: 407 1416 2520 3497 407
Size: 1009 1104 977 974
Fragments arranged by size:
1104 1009 977 974
AgeI A'CCGG_T
Cuts at: 2452 2452
Size: 4064
AlwNI CAG_nnn'CTG
Cuts at: 436 782 1369 2936 436
Size: 346 587 1567 1564
Fragments arranged by size:
1567 1564 587 346
ApaI G_GGCC'C
Cuts at: 503 503
Size: 4064
ApaLI G'TGCA_C
Cuts at: 2834 2834
Size: 4064
ApoI r'AATT_y
Cuts at: 1068 3417 3673 1068
Size: 2349 256 1459
Fragments arranged by size:
2349 1459 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4064
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
AvaI C'yCGr_G
Cuts at: 496 502 1084 496
Size: 6 582 3476
Fragments arranged by size:
3476 582 6
BamHI G'GATC_C
Cuts at: 1089 1089
Size: 4064
BanI G'GyrC_C
Cuts at: 308 426 448 556 1080 1494 1742 1777 308
Size: 118 22 108 524 414 248 35 2595
Fragments arranged by size:
2595 524 414 248 118 108 35 22
BanII G_rGCy'C
Cuts at: 217 503 1078 2108 217
Size: 286 575 1030 2173
Fragments arranged by size:
2173 1030 575 286
BbeI G_GCGC'C
Cuts at: 1498 1746 1498
Size: 248 3816
BbsI GAAGACnn'nnnn_
Cuts at: 125 514 125
Size: 389 3675
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4030
BciVI GTATCCnnnnn_n'
Cuts at: 1962 2723 4064 1962
Size: 761 1341 1962
Fragments arranged by size:
1962 1341 761
BclI T'GATC_A
Cuts at: 587 1584 587
Size: 997 3067
BglI GCCn_nnn'nGGC
Cuts at: 913 934 949 913
Size: 21 15 4028
Fragments arranged by size:
4028 21 15
BglII A'GATC_T
Cuts at: 1579 3616 1579
Size: 2037 2027
BlpI GC'TnA_GC
Cuts at: 1176 1176
Size: 4064
Bme1580I G_kGCm'C
Cuts at: 311 503 559 1689 1782 2838 311
Size: 192 56 1130 93 1056 1537
Fragments arranged by size:
1537 1130 1056 192 93 56
BmrI ACTGGGnnnn_n'
Cuts at: 1276 1357 1692 3836 1276
Size: 81 335 2144 1504
Fragments arranged by size:
2144 1504 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 528 2431 528
Size: 1903 2161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 534 617 775 352
Size: 182 83 158 3641
Fragments arranged by size:
3641 182 158 83
Bpu10I CC'TnA_GC
Cuts at: 995 995
Size: 4064
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1240 2119 2611 2909 1240
Size: 879 492 298 2395
Fragments arranged by size:
2395 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 499 1054 3768 499
Size: 555 2714 795
Fragments arranged by size:
2714 795 555
BsaAI yAC'GTr
Cuts at: 1417 1417
Size: 4064
BsaBI GATnn'nnATC
Cuts at: 6 176 1578 3615 6
Size: 170 1402 2037 455
Fragments arranged by size:
2037 1402 455 170
BsaHI Gr'CG_yC
Cuts at: 1495 1743 2445 3941 1495
Size: 248 702 1496 1618
Fragments arranged by size:
1618 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1253 1774 2452 2726 2873 3284 1253
Size: 521 678 274 147 411 2033
Fragments arranged by size:
2033 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 590 620 954 984 590
Size: 30 334 30 3670
Fragments arranged by size:
3670 334 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1036 1036
Size: 4064
BseYI C'CCAG_C
Cuts at: 324 990 1489 2247 2824 3320 324
Size: 666 499 758 577 496 1068
Fragments arranged by size:
1068 758 666 577 499 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 601
Size: 4064
BsiEI CG_ry'CG
Cuts at: 104 1606 1652 2860 104
Size: 1502 46 1208 1308
Fragments arranged by size:
1502 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1078 1856 2046 2838 1078
Size: 778 190 792 2304
Fragments arranged by size:
2304 792 778 190
BsmI GAATG_Cn'
Cuts at: 741 741
Size: 4064
BsmBI CGTCTCn'nnnn_
Cuts at: 862 862
Size: 4064
Bsp1286I G_dGCh'C
Cuts at: 217 311 503 559 1078 1689 1782 1856 2046
Size: 94 192 56 519 611 93 74 190
Cuts at: 2046 2108 2838 217
Size: 62 730 1443
Fragments arranged by size:
1443 730 611 519 192 190 94 93
74 62 56
BspEI T'CCGG_A
Cuts at: 3284 3284
Size: 4064
BspHI T'CATG_A
Cuts at: 3240 3240
Size: 4064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 669 1114 1328 1630 2011 669
Size: 445 214 302 381 2722
Fragments arranged by size:
2722 445 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2356 2410 3894 4059 50
Size: 2306 54 1484 165 55
Fragments arranged by size:
2306 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 452 774 1976 452
Size: 322 1202 2540
Fragments arranged by size:
2540 1202 322
BsrFI r'CCGG_y
Cuts at: 2062 2243 2452 2062
Size: 181 209 3674
Fragments arranged by size:
3674 209 181
BssSI C'ACGA_G
Cuts at: 2335 2693 2335
Size: 358 3706
BstAPI GCAn_nnn'nTGC
Cuts at: 1441 1441
Size: 4064
BstBI TT'CG_AA
Cuts at: 2425 2425
Size: 4064
BstXI CCAn_nnnn'nTGG
Cuts at: 490 490
Size: 4064
BstYI r'GATC_y
Cuts at: 1 233 1089 1556 1579 1914 2160 3172 3258
Size: 232 856 467 23 335 246 1012 86
Cuts at: 3258 3270 3616 1
Size: 12 346 449
Fragments arranged by size:
1012 856 467 449 346 335 246 232
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2014 2195 2358 2014
Size: 181 163 3720
Fragments arranged by size:
3720 181 163
BtsI GCAGTG_nn'
Cuts at: 1307 1307
Size: 4064
DraI TTT'AAA
Cuts at: 1063 1063
Size: 4064
DrdI GACnn_nn'nnGTC
Cuts at: 135 1770 2628 135
Size: 1635 858 1571
Fragments arranged by size:
1635 1571 858
EaeI y'GGCC_r
Cuts at: 416 1649 1823 2214 2241 3586 416
Size: 1233 174 391 27 1345 894
Fragments arranged by size:
1345 1233 894 391 174 27
EagI C'GGCC_G
Cuts at: 1649 1649
Size: 4064
EarI CTCTTCn'nnn_
Cuts at: 1007 2087 2297 1007
Size: 1080 210 2774
Fragments arranged by size:
2774 1080 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2272 2582 2728 3874 2272
Size: 310 146 1146 2462
Fragments arranged by size:
2462 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1076 1076
Size: 4064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 534 617 731 775 1889 2321 3068 3336
Size: 182 83 114 44 1114 432 747 268
Cuts at: 3336 352
Size: 1080
Fragments arranged by size:
1114 1080 747 432 268 182 114 83
44
EcoO109I rG'GnC_Cy
Cuts at: 387 1203 387
Size: 816 3248
EcoRI G'AATT_C
Cuts at: 1068 1068
Size: 4064
EcoRV GAT'ATC
Cuts at: 723 723
Size: 4064
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 531 927 2083 531
Size: 396 1156 2512
Fragments arranged by size:
2512 1156 396
FspI TGC'GCA
Cuts at: 292 1845 292
Size: 1553 2511
HaeII r_GCGC'y
Cuts at: 1498 1746 2768 3747 1498
Size: 248 1022 979 1815
Fragments arranged by size:
1815 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1034 1066 1906 1936 1938 1968 1034
Size: 32 840 30 2 30 3130
Fragments arranged by size:
3130 840 32 30 30 2
HincII GTy'rAC
Cuts at: 1103 1103
Size: 4064
KasI G'GCGC_C
Cuts at: 1494 1742 1494
Size: 248 3816
KpnI G_GTAC'C
Cuts at: 1084 1084
Size: 4064
MluI A'CGCG_T
Cuts at: 3497 3497
Size: 4064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 164 186 998 1007 2735 2919 164
Size: 22 812 9 1728 184 1309
Fragments arranged by size:
1728 1309 812 184 22 9
MscI TGG'CCA
Cuts at: 418 1825 418
Size: 1407 2657
MslI CAynn'nnrTG
Cuts at: 286 2053 2180 286
Size: 1767 127 2170
Fragments arranged by size:
2170 1767 127
MspA1I CmG'CkG
Cuts at: 433 585 967 1175 1489 1849 2862 3107 433
Size: 152 382 208 314 360 1013 245 1390
Fragments arranged by size:
1390 1013 382 360 314 245 208 152
NaeI GCC'GGC
Cuts at: 2245 2245
Size: 4064
NarI GG'CG_CC
Cuts at: 1495 1743 1495
Size: 248 3816
NgoMIV G'CCGG_C
Cuts at: 2243 2243
Size: 4064
NspI r_CATG'y
Cuts at: 243 411 639 1117 1617 2524 243
Size: 168 228 478 500 907 1783
Fragments arranged by size:
1783 907 500 478 228 168
PasI CC'CwG_GG
Cuts at: 113 113
Size: 4064
PciI A'CATG_T
Cuts at: 407 2520 407
Size: 2113 1951
PflMI CCAn_nnn'nTGG
Cuts at: 168 168
Size: 4064
PfoI T'CCnGG_A
Cuts at: 752 752
Size: 4064
PmeI GTTT'AAAC
Cuts at: 1063 1063
Size: 4064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3203 3235 3203
Size: 32 4032
PpuMI rG'GwC_Cy
Cuts at: 387 387
Size: 4064
PspOMI G'GGCC_C
Cuts at: 499 499
Size: 4064
PstI C_TGCA'G
Cuts at: 791 987 1111 1796 791
Size: 196 124 685 3059
Fragments arranged by size:
3059 685 196 124
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4064
PvuII CAG'CTG
Cuts at: 433 585 967 1489 1849 433
Size: 152 382 522 360 2648
Fragments arranged by size:
2648 522 382 360 152
RsrII CG'GwC_CG
Cuts at: 2259 2259
Size: 4064
SacI G_AGCT'C
Cuts at: 1078 1078
Size: 4064
SalI G'TCGA_C
Cuts at: 1101 1101
Size: 4064
SapI GCTCTTCn'nnn_
Cuts at: 2087 2297 2087
Size: 210 3854
SbfI CC_TGCA'GG
Cuts at: 1111 1111
Size: 4064
ScaI AGT'ACT
Cuts at: 3581 3581
Size: 4064
SfcI C'TryA_G
Cuts at: 33 787 983 1107 1792 2785 2976 33
Size: 754 196 124 685 993 191 1121
Fragments arranged by size:
1121 993 754 685 196 191 124
SfoI GGC'GCC
Cuts at: 1496 1744 1496
Size: 248 3816
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
SmaI CCC'GGG
Cuts at: 1086 1086
Size: 4064
SmlI C'TyrA_G
Cuts at: 151 1219 2134 2626 2888 151
Size: 1068 915 492 262 1327
Fragments arranged by size:
1327 1068 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4064
SphI G_CATG'C
Cuts at: 1117 1617 1117
Size: 500 3564
StuI AGG'CCT
Cuts at: 907 907
Size: 4064
StyI C'CwwG_G
Cuts at: 380 1007 1198 1552 380
Size: 627 191 354 2892
Fragments arranged by size:
2892 627 354 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1647 1780 2239 2448 1647
Size: 133 459 209 3263
Fragments arranged by size:
3263 459 209 133
TatI w'GTAC_w
Cuts at: 3579 3579
Size: 4064
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 590 622 954 986 3581 3613 590
Size: 32 332 32 2595 32 1041
Fragments arranged by size:
2595 1041 332 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1861 1861
Size: 4064
XbaI T'CTAG_A
Cuts at: 1095 1095
Size: 4064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1559 1559
Size: 4064
XmaI C'CCGG_G
Cuts at: 1084 1084
Size: 4064
XmnI GAAnn'nnTTC
Cuts at: 187 187
Size: 4064
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI BsiWI BsrGI BssHII
BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI