Restriction map for
pF1KB8213
(Circular) (Six-base) MAPSORT of: pF1KB8213.seq Check: 7381 from: 1 to: 3575
pF1KB8213 3575 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 613 613
Size: 3575
Acc65I G'GTAC_C
Cuts at: 591 591
Size: 3575
AclI AA'CG_TT
Cuts at: 3415 3415
Size: 3575
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 248 1400 1832 2579 2847 154
Size: 94 1152 432 747 268 882
Fragments arranged by size:
1152 882 747 432 268 94
AflIII A'CryG_T
Cuts at: 927 2031 3008 927
Size: 1104 977 1494
Fragments arranged by size:
1494 1104 977
AgeI A'CCGG_T
Cuts at: 1963 1963
Size: 3575
AleI CACnn'nnGTG
Cuts at: 460 460
Size: 3575
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 233 265 233
Size: 32 3543
AlwNI CAG_nnn'CTG
Cuts at: 880 2447 880
Size: 1567 2008
ApaLI G'TGCA_C
Cuts at: 2345 2345
Size: 3575
ApoI r'AATT_y
Cuts at: 579 2928 3184 579
Size: 2349 256 970
Fragments arranged by size:
2349 970 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3575
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3575
AvaI C'yCGr_G
Cuts at: 595 595
Size: 3575
BamHI G'GATC_C
Cuts at: 600 600
Size: 3575
BanI G'GyrC_C
Cuts at: 591 1005 1253 1288 591
Size: 414 248 35 2878
Fragments arranged by size:
2878 414 248 35
BanII G_rGCy'C
Cuts at: 589 1619 589
Size: 1030 2545
BbeI G_GCGC'C
Cuts at: 1009 1257 1009
Size: 248 3327
BciVI GTATCCnnnnn_n'
Cuts at: 1473 2234 3575 1473
Size: 761 1341 1473
Fragments arranged by size:
1473 1341 761
BclI T'GATC_A
Cuts at: 1095 1095
Size: 3575
BglII A'GATC_T
Cuts at: 1090 3127 1090
Size: 2037 1538
BlpI GC'TnA_GC
Cuts at: 687 687
Size: 3575
Bme1580I G_kGCm'C
Cuts at: 1200 1293 2349 1200
Size: 93 1056 2426
Fragments arranged by size:
2426 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 142 787 868 1203 3347 142
Size: 645 81 335 2144 370
Fragments arranged by size:
2144 645 370 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1942 1942
Size: 3575
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 1630 2122 2420 751
Size: 879 492 298 1906
Fragments arranged by size:
1906 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 428 3279 428
Size: 2851 724
BsaAI yAC'GTr
Cuts at: 928 928
Size: 3575
BsaBI GATnn'nnATC
Cuts at: 6 1089 3126 6
Size: 1083 2037 455
Fragments arranged by size:
2037 1083 455
BsaHI Gr'CG_yC
Cuts at: 1006 1254 1956 3452 1006
Size: 248 702 1496 1129
Fragments arranged by size:
1496 1129 702 248
BsaWI w'CCGG_w
Cuts at: 764 1285 1963 2237 2384 2795 764
Size: 521 678 274 147 411 1544
Fragments arranged by size:
1544 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1000 1758 2335 2831 1000
Size: 758 577 496 1744
Fragments arranged by size:
1744 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 308 172
Size: 136 3439
BsiEI CG_ry'CG
Cuts at: 104 1117 1163 2371 104
Size: 1013 46 1208 1308
Fragments arranged by size:
1308 1208 1013 46
BsiHKAI G_wGCw'C
Cuts at: 589 1367 1557 2349 589
Size: 778 190 792 1815
Fragments arranged by size:
1815 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 589 1200 1293 1367 1557 1619 2349 589
Size: 611 93 74 190 62 730 1815
Fragments arranged by size:
1815 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2795 2795
Size: 3575
BspHI T'CATG_A
Cuts at: 252 2751 252
Size: 2499 1076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 280 625 839 1141 1522 280
Size: 345 214 302 381 2333
Fragments arranged by size:
2333 381 345 302 214
BsrBI CCG'CTC
Cuts at: 50 1867 1921 3405 3570 50
Size: 1817 54 1484 165 55
Fragments arranged by size:
1817 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 372 438 1487 372
Size: 66 1049 2460
Fragments arranged by size:
2460 1049 66
BsrFI r'CCGG_y
Cuts at: 1573 1754 1963 1573
Size: 181 209 3185
Fragments arranged by size:
3185 209 181
BssSI C'ACGA_G
Cuts at: 1846 2204 1846
Size: 358 3217
BstAPI GCAn_nnn'nTGC
Cuts at: 952 952
Size: 3575
BstBI TT'CG_AA
Cuts at: 1936 1936
Size: 3575
BstYI r'GATC_y
Cuts at: 1 600 1067 1090 1425 1671 2683 2769 2781
Size: 599 467 23 335 246 1012 86 12
Cuts at: 2781 3127 1
Size: 346 449
Fragments arranged by size:
1012 599 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3575
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1525 1706 1869 1525
Size: 181 163 3231
Fragments arranged by size:
3231 181 163
BtsI GCAGTG_nn'
Cuts at: 818 818
Size: 3575
DraI TTT'AAA
Cuts at: 574 574
Size: 3575
DrdI GACnn_nn'nnGTC
Cuts at: 1281 2139 1281
Size: 858 2717
EaeI y'GGCC_r
Cuts at: 392 450 1160 1334 1725 1752 3097 392
Size: 58 710 174 391 27 1345 870
Fragments arranged by size:
1345 870 710 391 174 58 27
EagI C'GGCC_G
Cuts at: 1160 1160
Size: 3575
EarI CTCTTCn'nnn_
Cuts at: 335 1598 1808 335
Size: 1263 210 2102
Fragments arranged by size:
2102 1263 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1783 2093 2239 3385 1783
Size: 310 146 1146 1973
Fragments arranged by size:
1973 1146 310 146
EcoICRI GAG'CTC
Cuts at: 587 587
Size: 3575
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 248 1400 1832 2579 2847 154
Size: 94 1152 432 747 268 882
Fragments arranged by size:
1152 882 747 432 268 94
EcoO109I rG'GnC_Cy
Cuts at: 292 714 292
Size: 422 3153
EcoRI G'AATT_C
Cuts at: 579 579
Size: 3575
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1594 1594
Size: 3575
FspI TGC'GCA
Cuts at: 1356 1356
Size: 3575
HaeII r_GCGC'y
Cuts at: 1009 1257 2279 3258 1009
Size: 248 1022 979 1326
Fragments arranged by size:
1326 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1417 1447 1449 1479 1417
Size: 30 2 30 3513
Fragments arranged by size:
3513 30 30 2
HincII GTy'rAC
Cuts at: 614 614
Size: 3575
KasI G'GCGC_C
Cuts at: 1005 1253 1005
Size: 248 3327
KpnI G_GTAC'C
Cuts at: 595 595
Size: 3575
MluI A'CGCG_T
Cuts at: 3008 3008
Size: 3575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 333 2246 2430 333
Size: 1913 184 1478
Fragments arranged by size:
1913 1478 184
MscI TGG'CCA
Cuts at: 1336 1336
Size: 3575
MslI CAynn'nnrTG
Cuts at: 251 460 1564 1691 251
Size: 209 1104 127 2135
Fragments arranged by size:
2135 1104 209 127
MspA1I CmG'CkG
Cuts at: 686 1000 1360 2373 2618 686
Size: 314 360 1013 245 1643
Fragments arranged by size:
1643 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1756 1756
Size: 3575
NarI GG'CG_CC
Cuts at: 1006 1254 1006
Size: 248 3327
NcoI C'CATG_G
Cuts at: 107 107
Size: 3575
NgoMIV G'CCGG_C
Cuts at: 1754 1754
Size: 3575
NspI r_CATG'y
Cuts at: 352 628 1128 2035 352
Size: 276 500 907 1892
Fragments arranged by size:
1892 907 500 276
PciI A'CATG_T
Cuts at: 2031 2031
Size: 3575
PmeI GTTT'AAAC
Cuts at: 574 574
Size: 3575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2714 2746 2714
Size: 32 3543
PpuMI rG'GwC_Cy
Cuts at: 292 292
Size: 3575
PstI C_TGCA'G
Cuts at: 622 1307 622
Size: 685 2890
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3575
PvuII CAG'CTG
Cuts at: 1000 1360 1000
Size: 360 3215
RsrII CG'GwC_CG
Cuts at: 1770 1770
Size: 3575
SacI G_AGCT'C
Cuts at: 589 589
Size: 3575
SalI G'TCGA_C
Cuts at: 612 612
Size: 3575
SapI GCTCTTCn'nnn_
Cuts at: 1598 1808 1598
Size: 210 3365
SbfI CC_TGCA'GG
Cuts at: 622 622
Size: 3575
ScaI AGT'ACT
Cuts at: 3092 3092
Size: 3575
SfcI C'TryA_G
Cuts at: 33 618 1303 2296 2487 33
Size: 585 685 993 191 1121
Fragments arranged by size:
1121 993 685 585 191
SfoI GGC'GCC
Cuts at: 1007 1255 1007
Size: 248 3327
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3575
SmaI CCC'GGG
Cuts at: 597 597
Size: 3575
SmlI C'TyrA_G
Cuts at: 730 1645 2137 2399 730
Size: 915 492 262 1906
Fragments arranged by size:
1906 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3575
SphI G_CATG'C
Cuts at: 628 1128 628
Size: 500 3075
StuI AGG'CCT
Cuts at: 233 233
Size: 3575
StyI C'CwwG_G
Cuts at: 107 709 1063 107
Size: 602 354 2619
Fragments arranged by size:
2619 602 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1158 1291 1750 1959 1158
Size: 133 459 209 2774
Fragments arranged by size:
2774 459 209 133
TatI w'GTAC_w
Cuts at: 511 3090 511
Size: 2579 996
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 449 481 3092 3124 449
Size: 32 2611 32 900
Fragments arranged by size:
2611 900 32 32
Tth111I GACn'n_nGTC
Cuts at: 1372 1372
Size: 3575
XbaI T'CTAG_A
Cuts at: 606 606
Size: 3575
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 200 445 1070 200
Size: 245 625 2705
Fragments arranged by size:
2705 625 245
XmaI C'CCGG_G
Cuts at: 595 595
Size: 3575
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI