Restriction map for
pF1KB8203
(Circular) (Six-base) MAPSORT of: pF1KB8203.seq Check: 2905 from: 1 to: 4022
pF1KB8203 4022 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 896 896
Size: 4022
AccI GT'mk_AC
Cuts at: 1060 1060
Size: 4022
Acc65I G'GTAC_C
Cuts at: 1038 1038
Size: 4022
AclI AA'CG_TT
Cuts at: 3862 3862
Size: 4022
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 1847 2279 3026 3294 340
Size: 1507 432 747 268 1068
Fragments arranged by size:
1507 1068 747 432 268
AfeI AGC'GCT
Cuts at: 519 544 519
Size: 25 3997
AflIII A'CryG_T
Cuts at: 1374 2478 3455 1374
Size: 1104 977 1941
Fragments arranged by size:
1941 1104 977
AgeI A'CCGG_T
Cuts at: 2410 2410
Size: 4022
AhdI GACnn_n'nnGTC
Cuts at: 381 381
Size: 4022
AleI CACnn'nnGTG
Cuts at: 662 662
Size: 4022
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 932 964 932
Size: 32 3990
AlwNI CAG_nnn'CTG
Cuts at: 345 492 1327 2894 345
Size: 147 835 1567 1473
Fragments arranged by size:
1567 1473 835 147
ApaI G_GGCC'C
Cuts at: 693 693
Size: 4022
ApaLI G'TGCA_C
Cuts at: 2792 2792
Size: 4022
ApoI r'AATT_y
Cuts at: 1026 3375 3631 1026
Size: 2349 256 1417
Fragments arranged by size:
2349 1417 256
AscI GG'CGCG_CC
Cuts at: 360 360
Size: 4022
AseI AT'TA_AT
Cuts at: 20 20
Size: 4022
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4022
AvaI C'yCGr_G
Cuts at: 166 177 1042 166
Size: 11 865 3146
Fragments arranged by size:
3146 865 11
BamHI G'GATC_C
Cuts at: 1047 1047
Size: 4022
BanI G'GyrC_C
Cuts at: 1038 1452 1700 1735 1038
Size: 414 248 35 3325
Fragments arranged by size:
3325 414 248 35
BanII G_rGCy'C
Cuts at: 178 194 693 928 1036 2066 178
Size: 16 499 235 108 1030 2134
Fragments arranged by size:
2134 1030 499 235 108 16
BbeI G_GCGC'C
Cuts at: 1456 1704 1456
Size: 248 3774
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 190 224 858 892 190
Size: 34 634 34 3320
Fragments arranged by size:
3320 634 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1920 2681 4022 1920
Size: 761 1341 1920
Fragments arranged by size:
1920 1341 761
BclI T'GATC_A
Cuts at: 1542 1542
Size: 4022
BglI GCCn_nnn'nGGC
Cuts at: 697 697
Size: 4022
BglII A'GATC_T
Cuts at: 1537 3574 1537
Size: 2037 1985
BlpI GC'TnA_GC
Cuts at: 1134 1134
Size: 4022
Bme1580I G_kGCm'C
Cuts at: 693 1647 1740 2796 693
Size: 954 93 1056 1919
Fragments arranged by size:
1919 1056 954 93
BmgBI CAC'GTC
Cuts at: 684 684
Size: 4022
BmrI ACTGGGnnnn_n'
Cuts at: 842 1234 1315 1650 3794 842
Size: 392 81 335 2144 1070
Fragments arranged by size:
2144 1070 392 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 609 2389 609
Size: 1780 2242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 442 588 388
Size: 54 146 3822
Fragments arranged by size:
3822 146 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 1198 2077 2569 2867 178
Size: 1020 879 492 298 1333
Fragments arranged by size:
1333 1020 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3726 3726
Size: 4022
BsaAI yAC'GTr
Cuts at: 1375 1375
Size: 4022
BsaBI GATnn'nnATC
Cuts at: 6 1536 3573 6
Size: 1530 2037 455
Fragments arranged by size:
2037 1530 455
BsaHI Gr'CG_yC
Cuts at: 1453 1701 2403 3899 1453
Size: 248 702 1496 1576
Fragments arranged by size:
1576 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1211 1732 2410 2684 2831 3242 1211
Size: 521 678 274 147 411 1991
Fragments arranged by size:
1991 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 574 895 136
Size: 438 321 3263
Fragments arranged by size:
3263 438 321
BseYI C'CCAG_C
Cuts at: 144 514 692 1001 1447 2205 2782 3278 144
Size: 370 178 309 446 758 577 496 888
Fragments arranged by size:
888 758 577 496 446 370 309 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 574
Size: 4022
BsiEI CG_ry'CG
Cuts at: 104 258 867 1564 1610 2818 104
Size: 154 609 697 46 1208 1308
Fragments arranged by size:
1308 1208 697 609 154 46
BsiHKAI G_wGCw'C
Cuts at: 194 887 1036 1814 2004 2796 194
Size: 693 149 778 190 792 1420
Fragments arranged by size:
1420 792 778 693 190 149
BsmI GAATG_Cn'
Cuts at: 982 982
Size: 4022
Bsp1286I G_dGCh'C
Cuts at: 178 194 693 887 928 1036 1647 1740 1814
Size: 16 499 194 41 108 611 93 74
Cuts at: 1814 2004 2066 2796 178
Size: 190 62 730 1404
Fragments arranged by size:
1404 730 611 499 194 190 108 93
74 62 41 16
BspEI T'CCGG_A
Cuts at: 3242 3242
Size: 4022
BspHI T'CATG_A
Cuts at: 3198 3198
Size: 4022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 896 1072 1286 1588 1969 896
Size: 176 214 302 381 2949
Fragments arranged by size:
2949 381 302 214 176
BsrBI CCG'CTC
Cuts at: 50 564 2314 2368 3852 4017 50
Size: 514 1750 54 1484 165 55
Fragments arranged by size:
1750 1484 514 165 55 54
BsrDI GCAATG_nn'
Cuts at: 611 1934 611
Size: 1323 2699
BsrFI r'CCGG_y
Cuts at: 356 2020 2201 2410 356
Size: 1664 181 209 1968
Fragments arranged by size:
1968 1664 209 181
BssHII G'CGCG_C
Cuts at: 182 360 182
Size: 178 3844
BssSI C'ACGA_G
Cuts at: 334 658 2293 2651 334
Size: 324 1635 358 1705
Fragments arranged by size:
1705 1635 358 324
BstAPI GCAn_nnn'nTGC
Cuts at: 1399 1399
Size: 4022
BstBI TT'CG_AA
Cuts at: 525 2383 525
Size: 1858 2164
BstYI r'GATC_y
Cuts at: 1 1047 1514 1537 1872 2118 3130 3216 3228
Size: 1046 467 23 335 246 1012 86 12
Cuts at: 3228 3574 1
Size: 346 449
Fragments arranged by size:
1046 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 617 617
Size: 4022
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 432 1972 2153 2316 432
Size: 1540 181 163 2138
Fragments arranged by size:
2138 1540 181 163
BtsI GCAGTG_nn'
Cuts at: 418 1265 418
Size: 847 3175
DraI TTT'AAA
Cuts at: 1021 1021
Size: 4022
DrdI GACnn_nn'nnGTC
Cuts at: 682 1728 2586 682
Size: 1046 858 2118
Fragments arranged by size:
2118 1046 858
EaeI y'GGCC_r
Cuts at: 255 284 347 476 1607 1781 2172 2199 3544
Size: 29 63 129 1131 174 391 27 1345
Cuts at: 3544 255
Size: 733
Fragments arranged by size:
1345 1131 733 391 174 129 63 29
27
EagI C'GGCC_G
Cuts at: 255 1607 255
Size: 1352 2670
EarI CTCTTCn'nnn_
Cuts at: 166 527 635 763 2045 2255 166
Size: 361 108 128 1282 210 1933
Fragments arranged by size:
1933 1282 361 210 128 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 173 748 2230 2540 2686 3832 173
Size: 575 1482 310 146 1146 363
Fragments arranged by size:
1482 1146 575 363 310 146
EcoICRI GAG'CTC
Cuts at: 192 1034 192
Size: 842 3180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 388 442 588 1847 2279 3026 3294 340
Size: 48 54 146 1259 432 747 268 1068
Fragments arranged by size:
1259 1068 747 432 268 146 54 48
EcoNI CCTnn'n_nnAGG
Cuts at: 478 934 478
Size: 456 3566
EcoO109I rG'GnC_Cy
Cuts at: 342 1161 342
Size: 819 3203
EcoRI G'AATT_C
Cuts at: 1026 1026
Size: 4022
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 414 831 2041 414
Size: 417 1210 2395
Fragments arranged by size:
2395 1210 417
FspI TGC'GCA
Cuts at: 1803 1803
Size: 4022
HaeII r_GCGC'y
Cuts at: 152 521 546 1456 1704 2726 3705 152
Size: 369 25 910 248 1022 979 469
Fragments arranged by size:
1022 979 910 469 369 248 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 367 399 1864 1894 1896 1926 367
Size: 32 1465 30 2 30 2463
Fragments arranged by size:
2463 1465 32 30 30 2
HincII GTy'rAC
Cuts at: 1061 1061
Size: 4022
KasI G'GCGC_C
Cuts at: 1452 1700 1452
Size: 248 3774
KpnI G_GTAC'C
Cuts at: 1042 1042
Size: 4022
MluI A'CGCG_T
Cuts at: 3455 3455
Size: 4022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2693 2877 2693
Size: 184 3838
MscI TGG'CCA
Cuts at: 478 1783 478
Size: 1305 2717
MslI CAynn'nnrTG
Cuts at: 662 2011 2138 662
Size: 1349 127 2546
Fragments arranged by size:
2546 1349 127
MspA1I CmG'CkG
Cuts at: 279 420 489 619 1133 1447 1807 2820 3065
Size: 141 69 130 514 314 360 1013 245
Cuts at: 3065 279
Size: 1236
Fragments arranged by size:
1236 1013 514 360 314 245 141 130
69
NaeI GCC'GGC
Cuts at: 358 2203 358
Size: 1845 2177
NarI GG'CG_CC
Cuts at: 1453 1701 1453
Size: 248 3774
NgoMIV G'CCGG_C
Cuts at: 356 2201 356
Size: 1845 2177
NruI TCG'CGA
Cuts at: 532 532
Size: 4022
NspI r_CATG'y
Cuts at: 1075 1575 2482 1075
Size: 500 907 2615
Fragments arranged by size:
2615 907 500
PciI A'CATG_T
Cuts at: 2478 2478
Size: 4022
PmeI GTTT'AAAC
Cuts at: 1021 1021
Size: 4022
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3161 3193 3161
Size: 32 3990
PspOMI G'GGCC_C
Cuts at: 689 689
Size: 4022
PstI C_TGCA'G
Cuts at: 461 893 1069 1754 461
Size: 432 176 685 2729
Fragments arranged by size:
2729 685 432 176
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4022
PvuII CAG'CTG
Cuts at: 420 1447 1807 420
Size: 1027 360 2635
Fragments arranged by size:
2635 1027 360
RsrII CG'GwC_CG
Cuts at: 2217 2217
Size: 4022
SacI G_AGCT'C
Cuts at: 194 1036 194
Size: 842 3180
SacII CC_GC'GG
Cuts at: 620 620
Size: 4022
SalI G'TCGA_C
Cuts at: 1059 1059
Size: 4022
SapI GCTCTTCn'nnn_
Cuts at: 166 527 2045 2255 166
Size: 361 1518 210 1933
Fragments arranged by size:
1933 1518 361 210
SbfI CC_TGCA'GG
Cuts at: 1069 1069
Size: 4022
ScaI AGT'ACT
Cuts at: 3539 3539
Size: 4022
SfcI C'TryA_G
Cuts at: 33 457 889 1065 1750 2743 2934 33
Size: 424 432 176 685 993 191 1121
Fragments arranged by size:
1121 993 685 432 424 191 176
SfiI GGCCn_nnn'nGGCC
Cuts at: 697 697
Size: 4022
SfoI GGC'GCC
Cuts at: 1454 1702 1454
Size: 248 3774
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4022
SmaI CCC'GGG
Cuts at: 1044 1044
Size: 4022
SmlI C'TyrA_G
Cuts at: 193 1177 2092 2584 2846 193
Size: 984 915 492 262 1369
Fragments arranged by size:
1369 984 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4022
SphI G_CATG'C
Cuts at: 1075 1575 1075
Size: 500 3522
StuI AGG'CCT
Cuts at: 700 700
Size: 4022
StyI C'CwwG_G
Cuts at: 479 1156 1510 479
Size: 677 354 2991
Fragments arranged by size:
2991 677 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 993 1605 1738 2197 2406 993
Size: 612 133 459 209 2609
Fragments arranged by size:
2609 612 459 209 133
TatI w'GTAC_w
Cuts at: 725 3537 725
Size: 2812 1210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3539 3571 3539
Size: 32 3990
Tth111I GACn'n_nGTC
Cuts at: 1819 1819
Size: 4022
XbaI T'CTAG_A
Cuts at: 1053 1053
Size: 4022
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1517 1517
Size: 4022
XmaI C'CCGG_G
Cuts at: 1042 1042
Size: 4022
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NruI
NspI PciI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AlfI AvrII BaeI BbsI BbvCI BfrBI
BmtI Bpu10I BsaXI BsiWI BsmBI BsrGI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SanDI SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI