Restriction map for
pF1KB8202
(Circular) (Six-base) MAPSORT of: pF1KB8202.seq Check: 8376 from: 1 to: 3695
pF1KB8202 3695 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 733 733
Size: 3695
Acc65I G'GTAC_C
Cuts at: 711 711
Size: 3695
AclI AA'CG_TT
Cuts at: 3535 3535
Size: 3695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1520 1952 2699 2967 1520
Size: 432 747 268 2248
Fragments arranged by size:
2248 747 432 268
AflIII A'CryG_T
Cuts at: 1047 2151 3128 1047
Size: 1104 977 1614
Fragments arranged by size:
1614 1104 977
AgeI A'CCGG_T
Cuts at: 2083 2083
Size: 3695
AlwNI CAG_nnn'CTG
Cuts at: 286 1000 2567 286
Size: 714 1567 1414
Fragments arranged by size:
1567 1414 714
ApaLI G'TGCA_C
Cuts at: 2465 2465
Size: 3695
ApoI r'AATT_y
Cuts at: 114 391 699 3048 3304 114
Size: 277 308 2349 256 505
Fragments arranged by size:
2349 505 308 277 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3695
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
AvaI C'yCGr_G
Cuts at: 715 715
Size: 3695
BamHI G'GATC_C
Cuts at: 663 720 663
Size: 57 3638
BanI G'GyrC_C
Cuts at: 711 1125 1373 1408 711
Size: 414 248 35 2998
Fragments arranged by size:
2998 414 248 35
BanII G_rGCy'C
Cuts at: 138 709 1739 138
Size: 571 1030 2094
Fragments arranged by size:
2094 1030 571
BbeI G_GCGC'C
Cuts at: 1129 1377 1129
Size: 248 3447
BciVI GTATCCnnnnn_n'
Cuts at: 1593 2354 3695 1593
Size: 761 1341 1593
Fragments arranged by size:
1593 1341 761
BclI T'GATC_A
Cuts at: 356 1215 356
Size: 859 2836
BfrBI ATG'CAT
Cuts at: 606 606
Size: 3695
BglII A'GATC_T
Cuts at: 1210 3247 1210
Size: 2037 1658
BlpI GC'TnA_GC
Cuts at: 807 807
Size: 3695
Bme1580I G_kGCm'C
Cuts at: 1320 1413 2469 1320
Size: 93 1056 2546
Fragments arranged by size:
2546 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 680 907 988 1323 3467 680
Size: 227 81 335 2144 908
Fragments arranged by size:
2144 908 335 227 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2062 2062
Size: 3695
Bpu10I CC'TnA_GC
Cuts at: 137 137
Size: 3695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 871 1750 2242 2540 871
Size: 879 492 298 2026
Fragments arranged by size:
2026 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3399 3399
Size: 3695
BsaAI yAC'GTr
Cuts at: 1048 1048
Size: 3695
BsaBI GATnn'nnATC
Cuts at: 6 1209 3246 6
Size: 1203 2037 455
Fragments arranged by size:
2037 1203 455
BsaHI Gr'CG_yC
Cuts at: 1126 1374 2076 3572 1126
Size: 248 702 1496 1249
Fragments arranged by size:
1496 1249 702 248
BsaWI w'CCGG_w
Cuts at: 884 1405 2083 2357 2504 2915 884
Size: 521 678 274 147 411 1664
Fragments arranged by size:
1664 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 246 276 246
Size: 30 3665
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 251 251
Size: 3695
BseYI C'CCAG_C
Cuts at: 1120 1878 2455 2951 1120
Size: 758 577 496 1864
Fragments arranged by size:
1864 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1237 1283 2491 104
Size: 1133 46 1208 1308
Fragments arranged by size:
1308 1208 1133 46
BsiHKAI G_wGCw'C
Cuts at: 709 1487 1677 2469 709
Size: 778 190 792 1935
Fragments arranged by size:
1935 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 138 709 1320 1413 1487 1677 1739 2469 138
Size: 571 611 93 74 190 62 730 1364
Fragments arranged by size:
1364 730 611 571 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2915 2915
Size: 3695
BspHI T'CATG_A
Cuts at: 308 2871 308
Size: 2563 1132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 449 745 959 1261 1642 449
Size: 296 214 302 381 2502
Fragments arranged by size:
2502 381 302 296 214
BsrBI CCG'CTC
Cuts at: 50 491 1987 2041 3525 3690 50
Size: 441 1496 54 1484 165 55
Fragments arranged by size:
1496 1484 441 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1607 1607
Size: 3695
BsrFI r'CCGG_y
Cuts at: 556 1693 1874 2083 556
Size: 1137 181 209 2168
Fragments arranged by size:
2168 1137 209 181
BssSI C'ACGA_G
Cuts at: 1966 2324 1966
Size: 358 3337
BstAPI GCAn_nnn'nTGC
Cuts at: 1072 1072
Size: 3695
BstBI TT'CG_AA
Cuts at: 2056 2056
Size: 3695
BstYI r'GATC_y
Cuts at: 1 663 720 1187 1210 1545 1791 2803 2889
Size: 662 57 467 23 335 246 1012 86
Cuts at: 2889 2901 3247 1
Size: 12 346 449
Fragments arranged by size:
1012 662 467 449 346 335 246 86
57 23 12
BtgI C'CryG_G
Cuts at: 107 248 254 107
Size: 141 6 3548
Fragments arranged by size:
3548 141 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1645 1826 1989 1645
Size: 181 163 3351
Fragments arranged by size:
3351 181 163
BtsI GCAGTG_nn'
Cuts at: 598 938 598
Size: 340 3355
DraI TTT'AAA
Cuts at: 694 694
Size: 3695
DrdI GACnn_nn'nnGTC
Cuts at: 1401 2259 1401
Size: 858 2837
EaeI y'GGCC_r
Cuts at: 251 554 1280 1454 1845 1872 3217 251
Size: 303 726 174 391 27 1345 729
Fragments arranged by size:
1345 729 726 391 303 174 27
EagI C'GGCC_G
Cuts at: 1280 1280
Size: 3695
EarI CTCTTCn'nnn_
Cuts at: 1718 1928 1718
Size: 210 3485
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 553 1903 2213 2359 3505 553
Size: 1350 310 146 1146 743
Fragments arranged by size:
1350 1146 743 310 146
EcoICRI GAG'CTC
Cuts at: 707 707
Size: 3695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1520 1952 2699 2967 1520
Size: 432 747 268 2248
Fragments arranged by size:
2248 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 539 539
Size: 3695
EcoO109I rG'GnC_Cy
Cuts at: 223 834 223
Size: 611 3084
EcoRI G'AATT_C
Cuts at: 699 699
Size: 3695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1714 1714
Size: 3695
FspI TGC'GCA
Cuts at: 1476 1476
Size: 3695
HaeII r_GCGC'y
Cuts at: 1129 1377 2399 3378 1129
Size: 248 1022 979 1446
Fragments arranged by size:
1446 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 441 473 1537 1567 1569 1599 441
Size: 32 1064 30 2 30 2537
Fragments arranged by size:
2537 1064 32 30 30 2
HincII GTy'rAC
Cuts at: 734 734
Size: 3695
KasI G'GCGC_C
Cuts at: 1125 1373 1125
Size: 248 3447
KpnI G_GTAC'C
Cuts at: 715 715
Size: 3695
MluI A'CGCG_T
Cuts at: 3128 3128
Size: 3695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2366 2550 2366
Size: 184 3511
MscI TGG'CCA
Cuts at: 1456 1456
Size: 3695
MslI CAynn'nnrTG
Cuts at: 1684 1811 1684
Size: 127 3568
MspA1I CmG'CkG
Cuts at: 250 806 1120 1480 2493 2738 250
Size: 556 314 360 1013 245 1207
Fragments arranged by size:
1207 1013 556 360 314 245
NaeI GCC'GGC
Cuts at: 558 1876 558
Size: 1318 2377
NarI GG'CG_CC
Cuts at: 1126 1374 1126
Size: 248 3447
NcoI C'CATG_G
Cuts at: 107 107
Size: 3695
NgoMIV G'CCGG_C
Cuts at: 556 1874 556
Size: 1318 2377
NsiI A_TGCA'T
Cuts at: 608 608
Size: 3695
NspI r_CATG'y
Cuts at: 748 1248 2155 748
Size: 500 907 2288
Fragments arranged by size:
2288 907 500
PciI A'CATG_T
Cuts at: 2151 2151
Size: 3695
PmeI GTTT'AAAC
Cuts at: 694 694
Size: 3695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2834 2866 2834
Size: 32 3663
PpuMI rG'GwC_Cy
Cuts at: 223 223
Size: 3695
PstI C_TGCA'G
Cuts at: 742 1427 742
Size: 685 3010
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3695
PvuII CAG'CTG
Cuts at: 1120 1480 1120
Size: 360 3335
RsrII CG'GwC_CG
Cuts at: 1890 1890
Size: 3695
SacI G_AGCT'C
Cuts at: 709 709
Size: 3695
SacII CC_GC'GG
Cuts at: 251 251
Size: 3695
SalI G'TCGA_C
Cuts at: 732 732
Size: 3695
SanDI GG'GwC_CC
Cuts at: 223 223
Size: 3695
SapI GCTCTTCn'nnn_
Cuts at: 1718 1928 1718
Size: 210 3485
SbfI CC_TGCA'GG
Cuts at: 742 742
Size: 3695
ScaI AGT'ACT
Cuts at: 3212 3212
Size: 3695
SfcI C'TryA_G
Cuts at: 33 738 1423 2416 2607 33
Size: 705 685 993 191 1121
Fragments arranged by size:
1121 993 705 685 191
SfoI GGC'GCC
Cuts at: 1127 1375 1127
Size: 248 3447
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
SmaI CCC'GGG
Cuts at: 717 717
Size: 3695
SmlI C'TyrA_G
Cuts at: 850 1765 2257 2519 850
Size: 915 492 262 2026
Fragments arranged by size:
2026 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3695
SphI G_CATG'C
Cuts at: 748 1248 748
Size: 500 3195
StyI C'CwwG_G
Cuts at: 107 829 1183 107
Size: 722 354 2619
Fragments arranged by size:
2619 722 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1278 1411 1870 2079 1278
Size: 133 459 209 2894
Fragments arranged by size:
2894 459 209 133
TatI w'GTAC_w
Cuts at: 3210 3210
Size: 3695
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 246 278 3212 3244 246
Size: 32 2934 32 697
Fragments arranged by size:
2934 697 32 32
Tth111I GACn'n_nGTC
Cuts at: 1492 1492
Size: 3695
XbaI T'CTAG_A
Cuts at: 726 726
Size: 3695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1190 1190
Size: 3695
XmaI C'CCGG_G
Cuts at: 715 715
Size: 3695
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmgBI BmtI BpmI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI